Screening and Identification of Yeasts Strains of Degradable Organic Acids in <i>Lonicera edulis</i>

LU Siyan; ZENG Xiangyu; WANG Xinyuan; MIAO Xiwen; WEN Liankui; HE Yang

doi:10.13386/j.issn1002-0306.2021020025

Volume 42 Issue 20

Oct. 2021

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Science and Technology of Food Industry > 2021 > 42(20): 126-133. > DOI: 10.13386/j.issn1002-0306.2021020025

LU Siyan, ZENG Xiangyu, WANG Xinyuan, et al. Screening and Identification of Yeasts Strains of Degradable Organic Acids in Lonicera edulis[J]. Science and Technology of Food Industry, 2021, 42(20): 126−133. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020025.

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Screening and Identification of Yeasts Strains of Degradable Organic Acids in Lonicera edulis

School of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China

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Received Date: February 04, 2021
Available Online: August 18, 2021

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Abstract

Microorganisms were isolated and purified from the fresh fruit of Changbai Mountain. The yeasts strains of degradable organic acids were screened and obtained. The acid reducing characteristics and tolerance were studied, and the biological identification was carried out. The results showed that five strains with acid reducing effect were selected from the fruits of Lonicera edulis. The acid reducing rates of B5 for malic acid, citric acid and tartaric acid were 42.60%±0.85%, 18.28%±0.80% and 13.09%±0.61% respectively. The strain B5 was identified as Zygosasaccharomyces bisporus by morphological observation, physiological and biochemical tests and 26S rDNA identification. The tolerance concentrations of strain B5 to glucose and SO₂ were 250 g/L and 400 mg/L, respectively, and its alcohol tolerance was 18% vol. The results of this experiment can provide a reference for the biological deacidification of fruit wine products.
- Lonicera edulis,
- degradation,
- organic acid,
- yeast,
- screening,
- identification

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[1]	迟丽华, 郑永春. 长白山野生蓝靛果的发展前景展望[J]. 吉林农业,2013(10):1. [Chi L H, Zheng Y C. Prospect of wildLonicera edulis in Changbai Mountain[J]. Jilin Agriculture,2013(10):1.
[2]	Bubola M, Đordano P, Karin K G, et al. Effects of fruit zone leaf removal on the concentrations of phenolic and organic acids in Istrian Malvasia grape juice and wine[J]. Food Technology & Biotechnology,2011,50(2):159−166.
[3]	李淑芹, 李延冰, 姜福臣, 等. 野生植物——蓝靛果营养成分研究[J]. 东北农业大学学报,1994(4):401−404. [Li S Q, Li Y B, Jiang F C, et al. Study on nutritional components of wild plantLonicera edulis[J]. Journal of Northeast Agricultural University,1994(4):401−404.
[4]	文连奎, 赵薇, 张微, 等. 果酒降酸技术研究进展[J]. 食品科学,2010,31(11):325−328. [Wen L K, Zhao W, Zhang W, et al. Research progress on acid reducing technology of fruit wine[J]. Food Science,2010,31(11):325−328.
[5]	姜欢笑, 郭建华, 时小棠, 等. 果酒中有机酸成分分析及降酸技术研究现状[J]. 现代食品,2020(9):10−14. [Jiang H X, Guo J H, Shi X T, et al. Analysis of organic acids in fruit wine and research status of acid reducing technology[J]. Modern Food,2020(9):10−14.
[6]	Vilela-Moura A, Schuller D, Mendes-Faia A, et al. The impact of acetate metabolism on yeast fermentative performance and wine quality: Reduction of volatile acidity of grape musts and wines[J]. Appl Microbiol Biotechnol,2011,89(2):271−280. doi: 10.1007/s00253-010-2898-3
[7]	杨创举, 何新, 朱丽霞. 慕萨莱思降糖、降酸优良酵母菌的筛选[J]. 食品与发酵科技,2020,56(3):22−30. [Yang C J, He X, Zhu L X. Screening of excellent Saccharomyces cerevisiae for hypoglycemic and acid reducing effect of mousareth[J]. Food and Fermentation Technology,2020,56(3):22−30.
[8]	宫祥博, 延海莹, 田迎樱, 等. 红树莓发酵果酒及其抗氧化活性[J]. 食品工业,2020,41(12):236−240. [Gong X B, Yan H Y, Tian Y Y, et al. Fermented red raspberry wine and its antioxidant activity[J]. Food Industry,2020,41(12):236−240.
[9]	Yokotsuka K, Nakanishi K, Seki T, et al. Studies on the production of kiwifruit wine: iii. quality of wines produced from fruits with various maturities[J]. Journal of the Brewing Society of Japan,2003,98(11):798−809. doi: 10.6013/jbrewsocjapan1988.98.798
[10]	Silva S, Ramon-Portugal F, Andrade P, et al. Malic acid consumption by dry immobilized cells of Schizosaccharomyces pombe[J]. American Journal of Enology & Viticulture,2003,54(1):50−55.
[11]	仇小妹, 王英, 董明盛, 等. 优良降酸酵母菌的筛选及发酵性能[J]. 食品科学,2014,35(5):160−164. [Qiu X M, Wang Y, Dong M S, et al. Screening and fermentation performance of excellent acid reducing yeast[J]. Food Science,2014,35(5):160−164. doi: 10.7506/spkx1002-6630-201405032
[12]	孙广仁, 张启昌, 董凤英, 等. 蓝靛果酵母发酵特性的研究[J]. 食品科学,2010,31(23):305−309. [Sun G R, Zhang Q C, Dong F Y, et al. Study on fermentation characteristics of Lonicera edulis[J]. Food Science,2010,31(23):305−309.
[13]	郝爱玲, 冯莉, 秦义, 等. 降解柠檬酸酵母菌的筛选及其发酵性能研究[J]. 中国食品学报,2018,18(11):72−80. [Hao A L, Feng L, Qin Y, et al. Screening of citric acid degrading yeast and its fermentation performance[J]. Chinese Journal of Food Science,2018,18(11):72−80.
[14]	胡小露. 降解柠檬酸酵母菌的筛选及在蓝莓酒中的应用[D]. 合肥: 安徽农业大学, 2012. Hu X L. Screening of citric acid degrading yeast and its application in blueberry wine[D]. Hefei: Anhui Agricultural University, 2012.
[15]	黄鹭强. 降酸酵母菌株的构建及其在枇杷酒酿造中的应用研究[D]. 福州: 福建农林大学, 2013. Huang L Q. Construction of acid reducing yeast strain and its application in loquat wine brewing[D]. Fuzhou: Fujian agriculture and Forestry University, 2013.
[16]	杨华, 刘亚娜, 郭德军. 红豆越橘酿酒优良降酸酵母的筛选及分离鉴定[J]. 食品科学,2016,37(9):175−180. [Yang H, Liu Y N, Guo D J. Screening and identification of excellent acid reducing yeast for red bean and blueberry brewing[J]. Food Science,2016,37(9):175−180. doi: 10.7506/spkx1002-6630-201609033
[17]	Ahmed M, Eldin, Z K, Nermeen H. Isolation and genetic identification of yeast producing biosurfactants, evaluated by different screening methods[J]. Microchemical Journal,2019,146:309−314. doi: 10.1016/j.microc.2019.01.020
[18]	陶森, 王雅玲, 叶林, 等. 腌鱼中耐盐酵母菌的分离鉴定及其生理生化特性[J]. 食品工业科技,2019,40(2):136−141, 147. [Tao S, Wang Y L, Ye L, et al. Isolation and identification of salt tolerant yeasts from salted fish and their physiological and biochemical characteristics[J]. Science and Technology of Food Industry,2019,40(2):136−141, 147.
[19]	Styczynska A K. In the search for novel wine yeast with deacidification activity[J]. Fermentation Technology,2012,1(2):2.
[20]	丁玉萍, 陶硕, 马金凤, 刘宇涵, 郭丹. 降酸酵母菌Yds-10最佳培养基的筛选[J]. 佳木斯大学学报(自然科学版),2018,36(6):937−940. [Ding Y P, Tao S, Ma J F, et al. Liu Yuhan, Guo Dan. Screening of the optimal medium for acidogenic yeast yds-10[J]. Journal of Jiamusi University (Natural Science Edition),2018,36(6):937−940.
[21]	Coelho E M, Padilha C V D S, Miskinis G A, et al. Simultaneous analysis of sugars and organic acids in wine and grape juices by HPLC: Method validation and characterization of products from northeast Brazil[J]. Journal of Food Composition and Analysis,2018,66:160−167. doi: 10.1016/j.jfca.2017.12.017
[22]	Scherer R, Rybka A C P, Ballus C A, et al. Validation of a HPLC method for simultaneous determination of main organic acids in fruits and juices[J]. Food Chemistry,2012,135(1):150−154. doi: 10.1016/j.foodchem.2012.03.111
[23]	Hutzler M, Koob J, Riedl R, et al. 5-Yeast identification and characterization[J]. Food Science,2015:65−104.
[24]	Belini V L, Suhr H, Wiedemann P. Online monitoring of the morphology of an industrial sugarcane biofuel yeast strain via in situ microscopy[J]. Journal of Microbiological Methods,2020,175:105973. doi: 10.1016/j.mimet.2020.105973
[25]	Velázquez E, Cruz-Sánchez J M, Rivas-Palá T, et al. YeastIdent-Food/ProleFood, a new system for the identification of food yeasts based on physiological and biochemical tests[J]. Food Microbiology,2001,18:637−646. doi: 10.1006/fmic.2001.0436
[26]	温新宇. 拮抗酵母菌的筛选、鉴定及其制剂对枇杷贮藏保鲜的影响[D]. 镇江: 江苏大学, 2016. Wen X Y. Screening and identification of Antagonistic Yeast and effects of its preparation on loquat storage[D]. Zhenjiang: Jiangsu University, 2016.
[27]	陈誉文, 洪梦楠, 李婧. 冰酒中的毕赤属和汉逊属酵母菌的筛选及其在葡萄酒酿造中的应用[J]. 食品科技,2021,46(1):8−14. [Chen Y W, Hong M N, Li J. Screening of Pichia and Hansenula yeasts in ice wine and their application in wine making[J]. Food Science and Technology,2021,46(1):8−14.
[28]	李迪, 李静媛. 蓝莓酒降酸酵母筛选鉴定及能力测定[J]. 酿酒科技,2017(7):46−51. [Li D, Li J Y. Screening, identification and capability determination of acid reducing yeast for blueberry wine[J]. Brewing Technology,2017(7):46−51.
[29]	张铎, 毛勇, 毛健, 等. 降柠檬酸菌株的筛选及鉴定[J]. 食品与机械,2017,33(5):3−7, 13. [Zhang D, Mao Y, Mao J, et al. Screening and identification of citric acid reducing strains[J]. Food and Machinery,2017,33(5):3−7, 13.
[30]	赵玉平, 王春霞, 杜连祥, 伍松陵. 酵母菌发酵降低山楂汁有机酸含量的研究[J]. 食品工业科技,2003(10):71−73. [Zhao Y P, Wang C X, Du L X, et al. Study on reducing organic acid content of hawthorn juice by yeast fermentation[J]. Food Industry Science and Technology,2003(10):71−73. doi: 10.3969/j.issn.1002-0306.2003.10.021
[31]	陈思睿, 唐琳琳, 冯建文, 等. 高效降解柠檬酸酵母菌的筛选鉴定及其在红树莓果汁中降酸特性[J]. 食品科学,2020,41(22):133−139. [Chen S R, Tang L L, Feng J W, et al. Screening and identification of citric acid degrading yeast and its acid reducing characteristics in red raspberry juice[J]. Food Science,2020,41(22):133−139. doi: 10.7506/spkx1002-6630-20191008-024
[32]	陈静, 程晓雨, 潘明, 等. 中国果酒生产技术研究现状及其产业未来发展趋势[J]. 食品工业科技,2017,38(2):383−389. [Chen J, Cheng X Y, Pan M, et al. Research status and future development trend of fruit wine production technology in China[J]. Food Industry Science and Technology,2017,38(2):383−389.
[33]	刘延岭, 邓林, 陶瑞霄. 降解苹果酸酵母菌的筛选及鉴定[J]. 食品科技,2020,45(3):8−12. [Liu Y L, Deng L, Tao R X. Screening and identification of malic acid degrading yeast[J]. Food Science and Technology,2020,45(3):8−12.
[34]	Cecilia O A, Melisa G F, Eugenia R. M, et al. Inheritance of winemaking stress factors tolerance in Saccharomyces uvarum/S. eubayanus × S. cerevisiae artificial hybrids[J]. International Journal of Food Microbiology, 2020, 320, 108500.

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