Optimization of Extraction Process of Polysaccharide from Black Corn Kernel by Response Surface Method and Analysis of Its Antioxidant Activity

WANG Yan; DUAN Xuewei; ZHANG Minjun; YANG Huiwen; LIU Bing; YOU Tianhui

doi:10.13386/j.issn1002-0306.2023020118

Volume 44 Issue 22

Nov. 2023

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Science and Technology of Food Industry > 2023 > 44(22): 191-200. > DOI: 10.13386/j.issn1002-0306.2023020118

WANG Yan, DUAN Xuewei, ZHANG Minjun, et al. Optimization of Extraction Process of Polysaccharide from Black Corn Kernel by Response Surface Method and Analysis of Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2023, 44(22): 191−200. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023020118.

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Optimization of Extraction Process of Polysaccharide from Black Corn Kernel by Response Surface Method and Analysis of Its Antioxidant Activity

1.
School of Nursing, Guangdong Pharmaceutical University, Guangzhou 510006, China
2.
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China

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Received Date: February 13, 2023
Available Online: September 12, 2023

Graphical Abstract

Abstract

Abstract

In order to explore the optimum extraction process of polysaccharide and antioxidant activity in vitro in black corn kernel. In this study, black corn kernel was used as raw material, ultrasonic-assisted extraction was applied to extract polysaccharides from black corn kernel. To explore the effects of ultrasonic power, solid-liquid ratio, extraction time, temperature and frequency on the yield of polysaccharide. The extraction process of polysaccharide from black corn kernel was optimized by response surface methodology. Besides, the antioxidant activity of the polysaccharide was investigated by measuring its scavenging ability on DPPH·, ABTS⁺·, and ·OH. The results showed that the extraction yield of polysaccharide from black corn kernel could reach up to 41.09%±0.59%, in these conditions: The solid-liquid ratio was 1:20 g/mL, the extraction temperature was 74 ℃, the extraction time was 60 min and the extraction frequency was 3 times. The IC₅₀ values of scavenging rates on DPPH·, ABTS⁺· and ·OH were 1.959, 1.529 and 0.3554 mg/mL, respectively. Moreover, the scavenging rates showed a certain dose-effect relationship with the sample concentration, indicating that the polysaccharide had a strong antioxidant activity, thus providing a theoretical basis for further research and utilization.
- black corn kernel,
- polysaccharide,
- ultrasonic,
- optimization of extraction process,
- antioxidant activity

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