Optimization of Flash Extraction Process and Antioxidant Activity of American Ginseng Flower Polysaccharides

KANG Zhongyu; ZHAO Daqing; YAO Jiajing; ZHAO Liming; HUANG Baotai; LIU Li; QI Bin

doi:10.13386/j.issn1002-0306.2023050278

Volume 45 Issue 7

Mar. 2024

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(7): 184-190. > DOI: 10.13386/j.issn1002-0306.2023050278

KANG Zhongyu, ZHAO Daqing, YAO Jiajing, et al. Optimization of Flash Extraction Process and Antioxidant Activity of American Ginseng Flower Polysaccharides[J]. Science and Technology of Food Industry, 2024, 45(7): 184−190. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050278.

Citation:

PDF (2444 KB)

Optimization of Flash Extraction Process and Antioxidant Activity of American Ginseng Flower Polysaccharides

1.
Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
2.
School of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China

More Information

Received Date: May 25, 2023
Available Online: January 23, 2024

Graphical Abstract

Abstract

Abstract

Objective: To study the optimal process conditions of flash extraction and antioxidant activity of American ginseng flower polysaccharides (AGFPs). Methods: American ginseng flower (AGF) as the raw material, the effects of extraction voltage, liquid-material ratio, and extraction time on the yield of AGFPs were explored. The flash extraction process of AGFPs was improved by response surface methodology. The scavenging effect on DPPH and hydroxy free radicals and total reduction capacity of AGFPs were determined to assess the antioxidant activity of AGFPs. Results: The optimal extraction conditions were determined as 130 V of extraction voltage, 30:1 mL/g of liquid-material ratio, and 100 s of extraction time, resulting in an AGFPs yield of 11.12%±0.23%, which agreed with the model prediction. The AGFPs exhibited significant scavenging effects on DPPH and hydroxyl radicals, with IC₅₀ values of 1.34 mg/mL and 1.42 mg/mL, respectively, and had a certain reducing power. Conclusion: These results suggested that flash extraction was an efficient and rapid method for obtaining AGFPs from AGF, and that AGFPs had promising antioxidant potential for further applications. This study can provide a theoretical basis for the development and application of AGF.
- American ginseng flower polysaccharides,
- flash extraction,
- process optimization,
- antioxidant activity,
- response surface methodology

FullText(HTML)

References (27)

References

[1]	国家药典委员会. 中华人民共和国药典-一部[M]. 北京:中国医药科技出版社, 2020:136. [Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China-Ⅰ[M]. Beijing:China Medical Science Press, 2020:136.] Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China-Ⅰ[M]. Beijing: China Medical Science Press, 2020: 136.
[2]	张正海, 雷慧霞, 钱佳奇, 等. 西洋参的引种简史[J]. 人参研究,2020,32(2):59−62. [ZHANG Zhenghai, LEI Huixia, QIAN Jiaqi, et al. Introduction history of Panax quinquefolium L J]. Ginseng Research,2020,32(2):59−62.
[3]	李春莲, 万楚军, 龚雪, 等. 西洋参种质资源及品种选育技术研究进展[J]. 西北农业学报,2021,30(3):321−332. [LI Chunlian, WAN Chujun, GONG Xue, et al. Advance of research in germplasm resources and breeding techiniques of american ginseng[J]. Acta Agriculturae Boreali-occidentalis Sinica,2021,30(3):321−332.] doi: 10.7606/j.issn.1004-1389.2021.03.001 LI Chunlian, WAN Chujun, GONG Xue, et al. Advance of research in germplasm resources and breeding techiniques of american ginseng[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2021, 30（3）: 321−332. doi: 10.7606/j.issn.1004-1389.2021.03.001
[4]	刘昌达. 西洋参花蕾化学成分的研究[D]. 沈阳:沈阳药科大学, 2008. [LIU Changda. Research on the chemical constituents of flower buds of Panax quinquefolium L D]. Shenyang:Shenyang Pharmaceutical University, 2008.
[5]	孟祥颖, 李向高, 张宏, 等. 国产西洋参花蕾中挥发油的分离与鉴定[J]. 分析化学,2001(5):542−545. [MENG Xiangying, LI Xianggao, ZHANG Hong, et al. Analysis of the volatile oils in the flowerbuds of Panax quinquefolium L doi: 10.3321/j.issn:0253-3820.2001.05.011 J]. Chinese Journal of Analytical Chemistry,2001(5):542−545. doi: 10.3321/j.issn:0253-3820.2001.05.011
[6]	许宁. 西洋参花主要化学成分的研究[D]. 长春:长春中医药大学, 2020. [XU Ning. Study on the primary chemical constituents of the fiower buds of Panax quinquefolium L D]. Changchun:Changchun University of Chinese Medicine, 2020.
[7]	NAKAMURA S, SUGIMOTO S, MATSUDA H, et al. Medicinal flowers. XVII. New dammarane-type triterpene glycosides from flower buds of American ginseng, Panax quinquefolium L.[J]. Chemical & Pharmaceutical Bulletin,2007,55(9):1342−1348.
[8]	SRITULARAK B, MORINAGA O, YUAN C, et al. Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies[J]. Journal of Natural Medicines,2009,63(3):360−363. doi: 10.1007/s11418-009-0332-x
[9]	刘雪莹, 赵雨, 刘莉, 等. 西洋参花多糖的提取和体外免疫调节作用的研究[J]. 食品工业,2018,39(1):23−25. [LIU Xueying, ZHAO Yu, LIU Li, et al. The extraction of polysaccharides from american ginseng flower and immunomodulation activities in vitro[J]. The Food Industry,2018,39(1):23−25.] LIU Xueying, ZHAO Yu, LIU Li, et al. The extraction of polysaccharides from american ginseng flower and immunomodulation activities in vitro[J]. The Food Industry, 2018, 39（1）: 23−25.
[10]	刘俊文, 徐美利, 徐冰, 等. 西洋参不同部位皂苷成分研究[J]. 天津中医药,2019,36(7):715−718. [LIU Junwen, XU Meili, XU Bing, et al. Study on saponin components in different parts of Panax quinquefolium L J]. Tianjin Journal of Traditional Chinese Medicine,2019,36(7):715−718.
[11]	YANG X S, WANG L J, DUNG C, et al. Optimization of ultrasonic-assisted extraction process of polysaccharides from American ginseng and evaluation of its immunostimulating activity[J]. Journal of Integrative Agriculture,2014,13(12):2807−2815. doi: 10.1016/S2095-3119(14)60785-1
[12]	陈军辉. 西洋参活性成分及其指纹图谱研究[D]. 南昌:南昌大学, 2005. [CHEN Junhui. Studies on the active chemical compositions of Panax quinquefolium L. and their fingerprint[D]. Nanchang:Nanchang University, 2005.] CHEN Junhui. Studies on the active chemical compositions of Panax quinquefolium L. and their fingerprint[D]. Nanchang: Nanchang University, 2005.
[13]	吴宪玲, 于晓红, 刘涛, 等. 响应面法优化西洋参多糖的酶解辅助提取工艺[J]. 食品工业,2016,37(1):118−122. [WU Xianling, YU Xiaohong, LIU Tao, et al. Optimization of enzymatic extraction of polysaccharides from Panax quinquefolium by response surface methodology[J]. The Food Industry,2016,37(1):118−122.] WU Xianling, YU Xiaohong, LIU Tao, et al. Optimization of enzymatic extraction of polysaccharides from Panax quinquefolium by response surface methodology[J]. The Food Industry, 2016, 37（1）: 118−122.
[14]	YU X N, YANG X S, CUI B, et al. Antioxidant and immunoregulatory activity of alkali-extractable polysaccharides from North American ginseng[J]. International Journal of Biological Macromolecules,2014,65:357−361. doi: 10.1016/j.ijbiomac.2014.01.046
[15]	崔胜文, 罗双群. 霍山石斛多糖的闪式提取工艺优化及降血糖作用研究[J]. 食品安全质量检测学报,2023,14(4):305−313. [CUI Shengwen, LUO Shuangqun. Optimization of homogenate extraction and hypoglycemic effects of polysaccharides from Dendrobium huoshanense[J]. Journal of Food Safety & Quality,2023,14(4):305−313.] doi: 10.3969/j.issn.2095-0381.2023.4.spaqzljcjs202304038 CUI Shengwen, LUO Shuangqun. Optimization of homogenate extraction and hypoglycemic effects of polysaccharides from Dendrobium huoshanense[J]. Journal of Food Safety & Quality, 2023, 14（4）: 305−313. doi: 10.3969/j.issn.2095-0381.2023.4.spaqzljcjs202304038
[16]	冉丽梅, 樊旭, 赵鹤鹏, 等. 闪式提取沙棘总黄酮工艺及其抗氧化活性研究[J]. 粮食与油脂,2023,36(1):110−114. [RAN Limei, FAN Xu, ZHAO Hepeng, et al. Study on the flash extraction technology and antioxidant activity of total flavonoids from Hippophae rhamnoides L doi: 10.3969/j.issn.1008-9578.2023.01.026 J]. Cereals & Oils,2023,36(1):110−114. doi: 10.3969/j.issn.1008-9578.2023.01.026
[17]	蔡永萍, 李阳杰. 薏苡仁多糖闪式提取工艺优化及其生物活性[J]. 食品研究与开发,2023,44(9):164−170. [CAI Yongping, LI Yangjie. Optimization of flash extraction process of polysaccharides in Coix lachryma seed and their bioactivity[J]. Food Research and Development,2023,44(9):164−170.] doi: 10.12161/j.issn.1005-6521.2023.09.023 CAI Yongping, LI Yangjie. Optimization of flash extraction process of polysaccharides in Coix lachryma seed and their bioactivity[J]. Food Research and Development, 2023, 44（9）: 164−170. doi: 10.12161/j.issn.1005-6521.2023.09.023
[18]	ZHU Y, WU M, LI X, et al. Flash extraction, characterization, and immunoenhancement activity of polysaccharide from Hippophae rhamnoides Linn[J]. Chemistry & Biodiversity,2023,20(3):e202200776.
[19]	GHOSH R, BRYANT D L, ARIVETT B A, et al. An acidic polysaccharide (AGC3) isolated from North American ginseng ( Panax quinquefolius) suspension culture as a potential immunomodulatory nutraceutical[J]. Current Research in Food Science,2020,3:207−216. doi: 10.1016/j.crfs.2020.07.002
[20]	郑艳. 西洋参多糖的分离纯化及结构研究[D]. 长春:东北师范大学, 2015. [ZHENG Yan. Purification and structure analysis of the polysaccharides from Panax quinquefolius[D]. Changchun:Northeast Normal University, 2015.] ZHENG Yan. Purification and structure analysis of the polysaccharides from Panax quinquefolius[D]. Changchun: Northeast Normal University, 2015.
[21]	SHEN S G, JIA S R, WU Y K, et al. Effect of culture conditions on the physicochemical properties and antioxidant activities of polysaccharides from Nostoc flagelliforme[J]. Carbohydrate Polymers,2018,198:426−433. doi: 10.1016/j.carbpol.2018.06.111
[22]	王海东, 韩荣欣, 张红印, 等. Osborne法分级提取五味子蛋白及抗氧化活性比较[J]. 食品工业科技,2021,42(23):59−65. [WANG Haidong, HAN Rongxin, ZHANG Hongyin, et al. Extraction of Schisandra chinensis protein by Osborne method and its antioxidant activity[J]. Science and Technology of Food Industry,2021,42(23):59−65.] WANG Haidong, HAN Rongxin, ZHANG Hongyin, et al. Extraction of Schisandra chinensis protein by Osborne method and its antioxidant activity[J]. Science and Technology of Food Industry, 2021, 42（23）: 59−65.
[23]	杨小梅, 苏林静, 黎小椿, 等. 荸荠皮皂苷的体外抗氧化活性研究[J]. 食品科技,2020,45(5):240−245. [YANG Xiaomei, SU Linjing, LI Xiaochun, et al. Research on antioxidative activity of chinese water chestnut peel saponins in vitro[J]. Food Science and Technology,2020,45(5):240−245.] YANG Xiaomei, SU Linjing, LI Xiaochun, et al. Research on antioxidative activity of chinese water chestnut peel saponins in vitro[J]. Food Science and Technology, 2020, 45（5）: 240−245.
[24]	东方, 王鑫, 王伟伟, 等. 芡实多糖闪式提取工艺优化及抗氧化活性研究[J]. 食品科技,2020,45(12):174−181. [DONG Fang, WANG Xin, WANG Weiwei, et al. Extraction optimization process by flash technology and antioxidant activity of Euryale polysaccharides[J]. Food Science and Technology,2020,45(12):174−181.] DONG Fang, WANG Xin, WANG Weiwei, et al. Extraction optimization process by flash technology and antioxidant activity of Euryale polysaccharides[J]. Food Science and Technology, 2020, 45（12）: 174−181.
[25]	MAZAREI F, JOOYANDEH H, NOSHAD M, et al. Polysaccharide of caper ( Capparis spinosa L.) leaf:Extraction optimization, antioxidant potential and antimicrobial activity[J]. International Journal of Biological Macromolecules,2017,95:224−231. doi: 10.1016/j.ijbiomac.2016.11.049
[26]	陈艳, 王杰, 李慧, 等. 黄精多糖的闪式提取及对乳酸菌发酵特性的影响[J]. 食品工业,2017,38(6):161−166. [CHEN Yan, WANG Jie, LI Hui, et al. Extraction of polysaccharide from polygonatum and its effects on fermentation characteristics of lactic acid bacteria[J]. The Food Industry,2017,38(6):161−166.] CHEN Yan, WANG Jie, LI Hui, et al. Extraction of polysaccharide from polygonatum and its effects on fermentation characteristics of lactic acid bacteria[J]. The Food Industry, 2017, 38（6）: 161−166.
[27]	曾俊, 西洋参多糖的提取分离、结构表征及抗氧化活性研究[D]. 哈尔滨:哈尔滨工业大学, 2021. [ZENG Jun, Extraction, isolation, structure characterization and antioxidant activity of polysaccharides derived from Panax quinquefolius[D]. Harbin:Harbin Institute of Technology, 2021.] ZENG Jun, Extraction, isolation, structure characterization and antioxidant activity of polysaccharides derived from Panax quinquefolius[D]. Harbin: Harbin Institute of Technology, 2021.

Supplements (1)

Supplements
Other Related Supplements

Cited By

Cited by

Periodical cited type(6)

1.	张琪婧，耿振甲，李敏，王俊秋，钱思颖，师聪. 覆盆子总黄酮的酶辅助闪式提取及其抗氧化、降血糖和降血脂活性分析. 粮食与油脂. 2025(01): 115-120+126 .
2.	田良良，殷志如，曹光昭，夏裕发，张晶晶，杨洪军. 基于代谢组学和生物信息学的西洋参三七丹参颗粒抗衰老作用及分子机制研究. 中国中药杂志. 2024(07): 1932-1946 .
3.	况慧云，孙萍东，林金元，杨立年，王慧，戴惠学，胡颖雄，郑洪建. 玉米须多糖的理化性质和体外生物活性研究. 上海农业学报. 2024(02): 24-30 .
4.	黎楚楚，徐霖，林长松. 响应面优化黄姜花根多糖提取工艺及其抗氧化活性分析. 饲料研究. 2024(11): 109-113 .
5.	苗琳，曲秋颖，段媛媛，衣少鹏，徐钰菲，王坤宁，宋政建. 西洋参多糖分离纯化及其生物活性研究进展. 中国果菜. 2024(10): 31-35 .
6.	徐清华，王国明，谢丽娟，宋莹莹，郭畅冰，冯惠柳，李健豪，于晶，徐芳菲. 西洋参花多糖的提取工艺优化及其含量测定. 数理医药学杂志. 2024(11): 806-814 .