WEI Yuping, ZHAO Yan, ZHANG Li, et al. Optimization of Ultrasonic-assisted Extraction of Phenylethanoside from Cistanche tubulosa and Its Antioxidant Activities[J]. Science and Technology of Food Industry, 2022, 43(13): 148−155. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090248.
Citation: WEI Yuping, ZHAO Yan, ZHANG Li, et al. Optimization of Ultrasonic-assisted Extraction of Phenylethanoside from Cistanche tubulosa and Its Antioxidant Activities[J]. Science and Technology of Food Industry, 2022, 43(13): 148−155. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090248.

Optimization of Ultrasonic-assisted Extraction of Phenylethanoside from Cistanche tubulosa and Its Antioxidant Activities

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  • Received Date: September 22, 2021
  • Available Online: May 17, 2022
  • In order to optimize the ultrasonic-assisted extraction process of phenylethanol glycosides from Cistanche tubulosa (Ct) in Xinjiang, box-behnken design was used to optimize the extraction conditions based on single factor experiments. Optimal conditions were the temperature of 40 ℃, the solid-liquid ratio of 1:41.7 g/mL and the ethanol concentration of 51%. The extraction yield under the optimal conditions was (14.36±0.12) mg/g. The extracts were mainly composed of echinacoside and verbascoside, the contents of which were (12.96±0.33) mg/g and (1.19±0.09) mg/g, respectively. The phenylethanoside extracts showed strong scavenging ability to hydroxyl radical and DPPH free radical, and the semi-inhibitory concentration (IC50) values were 60.196 mg/mL and 0.875 mg/mL, respectively. This study provides basic data for the extraction of phenylethanoside from Cistanche tubulosa in Xinjiang.
  • [1]
    屠鹏飞, 刘全儒. 管花肉苁蓉[J]. 生物学通报,2019,54(12):33. [TU F, LIU Q R. Cistanche tubulosa (Schenk) Wight[J]. Biology Bulletin,2019,54(12):33.

    TU F, LIU Q R. Cistanche tubulosa( Schenk) Wight[J]. Biology Bulletin, 2019, 54(12): 33.
    [2]
    路颖慧, 皮雯敏, 张悦, 等. 肉苁蓉和管花肉苁蓉的比较研究及市场现状分析[J]. 中国现代中药,2019,21(8):999−1005. [LU Y H, PI W M, ZHANG Y. et al. Comparative study and analysis of market status of Cistanche deserticoLa Y. C. Ma and Cistanche tubulosa (Schenk) Wigh[J]. China Modern Chinese Medicine,2019,21(8):999−1005.

    LU Y H, PI W M, ZHANG Y. et al. Comparative study and analysis of market status of Cistanche deserticoLa Y. C. Ma and Cistanche tubulosa(Schenk) Wigh[J]. China Modern Chinese Medicine, 2019, 21(8): 999-1005.
    [3]
    CAI R, YANG M, SHI Y, et al. Antifatigue activity of phenylethanoid-rich extract from Cistanche deserticola[J]. Phytotherapy Research,2010,24(2):313−315. doi: 10.1002/ptr.2927
    [4]
    JIANG Z, WANG J, LI X, et al. Echinacoside and Cistanche tubulosa R. wight ameliorate bisphenol a-induced testicular and sperm damage in rats through gonad axis regulated steroidogenic enzymes[J]. Journal of Ethnopharmacology,2016,193:321−328. doi: 10.1016/j.jep.2016.07.033
    [5]
    LI X, GUO C, YANG H, et al. Echinacoside ameliorates D-galactosamine plus lipopolysaccharide-induced acute liver injury in mice via inhibition of apoptosis and inflammation[J]. Scandinavian Journal of Gastroenterology,2014,49(8):993−1000. doi: 10.3109/00365521.2014.913190
    [6]
    LI J, LI J, AIPIRE A, et al. Phenylethanoid glycosides from Cistanche tubulosa inhibits the growth of B16-F10 cells both in vitro and in vivo by induction of apoptosis via mitochondria-dependent pathway[J]. Journal of Cancer,2016,7(13):1877−1887. doi: 10.7150/jca.15512
    [7]
    JIANG W, CHUNHUA M, SHUMIN W. Effects of acteoside on lipopolysaccharide-induced inflammation in acute lung injury via regulation of NF-κB pathway in vivo and in vitro[J]. Toxicology and Applied Pharmacology,2015,285(2):128−135. doi: 10.1016/j.taap.2015.04.004
    [8]
    ZHOU L, FENG Y, JIN Y, et al. Verbascoside promotes apoptosis by regulating HIPK2-p53 signaling in human colorectal cancer[J]. BMC Cancer,2014,14(1):747. doi: 10.1186/1471-2407-14-747
    [9]
    GAO L, PENG X, HOU S, et al. Memory enhancement of acteoside (Verbascoside) in a senescent mice model induced by combination of D-gal and AlCl3[J]. Phytotherapy Research,2015,29(8):1131−1136. doi: 10.1002/ptr.5357
    [10]
    MA Z, YANG Z, LU D, et al. Determination of boactive components of Cistanche deserticola (Roucongrong) by high-performance liquid chromatography with diode array and mass spectrometry detectors[J]. Analytical Letters,2014,47:2783−2794. doi: 10.1080/00032719.2014.924012
    [11]
    JIANG Y, TU P. Analysis of chemical constituents in Cistanche species[J]. Journal of Chromatography A,2009,1216(11):1970−1979. doi: 10.1016/j.chroma.2008.07.031
    [12]
    郭元亨. 荒漠肉苁蓉资源的综合利用[D] 北京: 中国科学院大学(中国科学院过程工程研究所), 2017.

    GUO Y H, CAO L L, ZHAO B, et al. Repairing effect of phenylethanol glycoside in Cistanche deserticola on alcohol-induced chronic liver injury[D]. Beijing: University of Chinese Academy of Sciences (Institute of Process Engineering, Chinese Academy of Sciences), 2017.
    [13]
    吴海虹, 玄国东, 刘春泉, 等. 肉苁蓉苯乙醇苷的纯化及其抗氧化活性研究[J]. 食品科学,2008(6):190−193. [WU H H, XUAN G D, LIU CH Q, et al. Purification and antioxidant activity of phenylethanol glycosides from Cistanche[J]. Food Science,2008(6):190−193. doi: 10.3321/j.issn:1002-6630.2008.06.038

    WU H H, XUAN G D, LIU CH Q, et al. Purification and antioxidant activity of phenylethanol glycosides from Cistanche[J]. Food Science, 2008(6): 190-193. doi: 10.3321/j.issn:1002-6630.2008.06.038
    [14]
    WANG L, DING H, YU H, et al. Cistanches Herba: Chemical constituents and pharmacological effects[J]. Chinese Herbal Medicines,2015,7(2):135−142. doi: 10.1016/S1674-6384(15)60017-X
    [15]
    杨建华, 胡君萍, 热娜·卡斯木, 等. 不同加工方法对肉苁蓉饮片苯乙醇苷类成分的影响[J]. 中药材,2010,33(5):691−693. [YANG J H, HU J P, RINA Kasmu, et al. Effect of different processing methods on the phenylethanol glycosides of Cistanche tablets[J]. Chinese Materia Medica,2010,33(5):691−693.

    YANG J H, HU J P, RINA Kasmu, et al. Effect of different processing methods on the phenylethanol glycosides of Cistanche tablets[J]. Chinese Materia Medica, 2010, 33(5): 691-693.
    [16]
    ZHU M, LU C, LI W. Transient exposure to echinacoside is sufficient to activate Trk signaling and protect neuronal cells from rotenone[J]. Journal of Neurochemistry,2013,124(4):571−580. doi: 10.1111/jnc.12103
    [17]
    郭元亨, 曹丽丽, 赵兵, 等. 荒漠肉苁蓉苯乙醇苷对酒精诱导的慢性肝损伤的修复作用[J]. 食品科学,2018,39(13):176−183. [GUO Y H, CAO L L, ZHAO B, et al. Reparative effects of phenylethanol glycosides from Cistanche deserticola on alcohol-induced chronic liver injury[J]. Food Science,2018,39(13):176−183. doi: 10.7506/spkx1002-6630-201813026

    GUO Y H, CAO L L, ZHAO B, et al. Reparative effects of phenylethanol glycosides from Cistanche deserticola on alcohol-induced chronic liver injury [J]. Food Science, 2018, 39(13): 176-183. doi: 10.7506/spkx1002-6630-201813026
    [18]
    CHEN H, JING F C, LI C L, et al. Echinacoside prevents the striatal extracellular levels of monoamine neurotransmitters from diminution in 6-hydroxydopamine lesion rats[J]. Journal of Ethnopharmacology,2007,114(3):285−289. doi: 10.1016/j.jep.2007.07.035
    [19]
    孔征, 毛乐静, 霍仕霞, 等. Box-Behnken响应面法优化管花肉苁蓉中毛蕊花糖苷的提取工艺研究[J]. 中国药房,2019,30(14):1970−1974. [KONG Z, MAO L J, HUO S X, et al. Optimization of the extraction process of mullein in Cistanche tubulosa by box-behnken response surface method[J]. Chinese Pharmacy,2019,30(14):1970−1974.

    KONG ZH, MAO L J, HUO SH X, et al. Optimization of the extraction process of mullein in Cistanche tubulosa by Box-Behnken response surface method[J]. Chinese Pharmacy, 2019, 30(14): 1970-1974.
    [20]
    FU Z F, HAN L F, ZHANG P, et al. Cistanche polysaccharides enhance echinacoside absorptionin vivo and affect the gut microbiota[J]. International Journal of Biological Macromolecules,2020,149:732−740. doi: 10.1016/j.ijbiomac.2020.01.216
    [21]
    JIN W, ZHOU T, LI G. Recent advances of modern sample preparation techniques for traditional Chinese medicines.[J]. Journal of Chromatography A,2019,1606:460377−460377. doi: 10.1016/j.chroma.2019.460377
    [22]
    WONG H SH, CHEN J H, LEONG P K, et al. Cistanches Herba reduces the weight gain in high fat diet-induced obese mice possibly through mitochondrial uncoupling[J]. Journal of Functional Foods,2014,10:292−304. doi: 10.1016/j.jff.2014.06.019
    [23]
    王毓杰. 管花肉苁蓉中苯乙醇苷的提取和大孔树脂纯化工艺研究[J]. 中草药,2014,45(16):2344−2348. [WANG Y J. Study on the extraction and macropore resin purification process of phenylethanol glycosides from Cistanche tubulosa[J]. Chinese Herbal Medicine,2014,45(16):2344−2348. doi: 10.7501/j.issn.0253-2670.2014.16.012

    WANG Y J. Study on the extraction and macropore resin purification process of phenylethanol glycosides from Cistanche tubulosa[J]. Chinese Herbal Medicine, 2014, 45(16): 2344-2348. doi: 10.7501/j.issn.0253-2670.2014.16.012
    [24]
    许欢欢, 胡君萍, 吴姗姗, 等. 肉苁蓉苯乙醇总苷传递体的制备及体外透皮实验[J]. 中国现代中药,2016,18(10):1322−1326. [XU H H, HU J P, WU S S, et al. Preparation and in vitro transdermal experiments of total glycoside delivery bodies of Cistanche phenylethanol[J]. China Modern Traditional Chinese Medicine,2016,18(10):1322−1326.

    XU H H, HU J P, WU S S, et al. Preparation and in vitro transdermal experiments of total glycoside delivery bodies of Cistanche phenylethanol[J]. China Modern Traditional Chinese Medicine, 2016, 18(10): 1322-1326.
    [25]
    刘伯言. 肉苁蓉苯乙醇苷的提取、纯化和抗氧化活性研究[D]. 北京: 北京林业大学, 2014.

    LIU B Y. Extraction, purification and antioxidant activity of phenylethanol glycosides from Cistanche[D]. Beijing: Beijing Forestry University, 2014.
    [26]
    丁慧玲, 许丹丹, 金天佑, 等. 超声-微波回流提取新疆管花肉苁蓉苯乙醇苷的工艺研究[J]. 食品工业科技,2016,37(12):301−305. [DING H L, XU D D, JIN T Y, et al. Process study of ultrasonic-microwave reflux extraction of phenylethanol glycosides from Cistanche tubulosa in Xinjiang[J]. Food Industry Science and Technology,2016,37(12):301−305.

    DING H L, XU D D, JIN T Y, et al. Process study of ultrasonic-microwave reflux extraction of phenylethanol glycosides from Cistanche tubulosa in Xinjiang[J]. Food Industry Science and Technology, 2016, 37(12): 301-305.
    [27]
    WANG Z, ZHOU F, QUAN Y. Antioxidant and immunological activity in vitro of polysaccharides from Phellinus nigricans mycelia[J]. International Journal of Biological Macromolecules,2014,64:139−143. doi: 10.1016/j.ijbiomac.2013.11.038
    [28]
    BABA S A, MALIK S A. Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume[J]. Journal of Taibah University for Science,2018,9(4):449−454.
    [29]
    ZHOU S, WU X, HUANG Y, et al. Microwave-assisted aqueous two-phase extraction of alkaloids from Radix Sophorae Tonkinensis with an ethanol/Na2HPO4 system: Process optimization, composition identification and quantification analysis[J]. Industrial Crops and Products,2018,122:316−328. doi: 10.1016/j.indcrop.2018.06.004
    [30]
    王丽楠, 陈君, 杨美华, 等. 不同初加工温度对肉苁蓉有效成分含量的影响[J]. 中国药房,2007(21):1620−1623. [WANG L N, CHEN J, YANG M H, et al. Effect of different processing temperatures on the content of active ingredients of Cistanche cistanche[J]. Chinese Pharmacy,2007(21):1620−1623. doi: 10.3969/j.issn.1001-0408.2007.21.008

    WANG L N, CHEN J, YANG M H, et al. Effect of different processing temperatures on the content of active ingredients of Cistanche cistanche[J]. Chinese Pharmacy, 2007(21): 1620 - 1623. doi: 10.3969/j.issn.1001-0408.2007.21.008
    [31]
    刘丽莎, 张清, 游林, 等. 管花肉苁蓉苯乙醇苷水溶媒提取技术研究[J]. 食品科技,2019,44(4):225−229. [LIU L S, ZHANG Q, YOU L, et. Study on the extraction technology of phenylethanol glycosides of Cistanche tubulosa by aqueous solvents[J]. Food Science and Technology,2019,44(4):225−229.

    LIU L SH, ZHANG Q, YOU L, et. Study on the extraction technology of phenylethanol glycosides of Cistanche tubulosa by aqueous solvents[J]. Food Science and Technology, 2019, 44(4): 225-229.
    [32]
    宋思圆, 苏平, 王丽娟, 等. 响应面试验优化超声提取黄秋葵花果胶多糖工艺及其体外抗氧化活性[J]. 食品科学,2017,38(2):283−289. [SONG S Y, SU P, WANG L J, et al. Optimization of ultrasonic-assisted extraction by response surface methodology and ntioxidant activities of pectic polysaccharide from okra flowers[J]. Food Science,2017,38(2):283−289. doi: 10.7506/spkx1002-6630-201702044

    SONG S Y, SU P, WANG L J, et al. Optimization of ultrasonic-assisted extraction by response surface methodology and ntioxidant activities of pectic polysaccharide from okra flowers [J]. Food Science, 2017, 38(2): 283-289. doi: 10.7506/spkx1002-6630-201702044
    [33]
    AHMAD-QASEM M H, CÁNOVAS J, BARRAJÓN-CATALÁN E, et al. Kinetic and compositional study of phenolic extraction from olive leaves (var. Serrana) by using power ultrasound[J]. Innovative Food Science & Emerging Technologies,2013,17:120−129.
    [34]
    LIU B, OUYANG J, YUAN X, et al. Adsorption properties and preparative separation of phenylethanoid glycosides from Cistanche deserticola by use of macroporous resins[J]. Journal of Chromatography B,2013,937:84−90. doi: 10.1016/j.jchromb.2013.08.018
    [35]
    蔡鸿, 鲍忠, 姜勇, 等. 不同产地管花肉苁蓉中有效成分的定量分析[J]. 中草药,2007(3):452−455. [CAI H, BAO ZH, JIANG Y, et al. Qualification of active constituents in Cistanche tubulosa from various habitats[J]. Chinese Traditional and Herbal Drugs,2007(3):452−455. doi: 10.3321/j.issn:0253-2670.2007.03.048

    CAI H, BAO ZH, JIANG Y, et al. Qualification of active constituents in Cistanche tubulosa from various habitats [J]. Chinese Traditional and Herbal Drugs, 2007(3): 452 - 455. doi: 10.3321/j.issn:0253-2670.2007.03.048
    [36]
    宫晓庆. 管花肉苁蓉苯乙醇总苷提纯工艺及其应用研究[D]. 北京: 北京石油化工学院, 2020.

    Gong X Q. Study on the purification process of total glycosides of Cistanche tubulosa phenylethanol and its application[D]. Beijing: Beijing Institute of Petrochemical Technology, 2020.
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