Optimization of the Extraction Process and <i>in Vitro</i> Antioxidant and Hypolipidemic Activities of Polysaccharides from <i>Sophora flavescens</i>

ZHAO Xiaoliang; MOU He; ZHANG Jing; ZHANG Weijie

doi:10.13386/j.issn1002-0306.2023120305

Volume 45 Issue 13

Jun. 2024

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Science and Technology of Food Industry > 2024 > 45(13): 212-220. > DOI: 10.13386/j.issn1002-0306.2023120305

ZHAO Xiaoliang, MOU He, ZHANG Jing, et al. Optimization of the Extraction Process and in Vitro Antioxidant and Hypolipidemic Activities of Polysaccharides from Sophora flavescens[J]. Science and Technology of Food Industry, 2024, 45(13): 212−220. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120305.

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Optimization of the Extraction Process and in Vitro Antioxidant and Hypolipidemic Activities of Polysaccharides from Sophora flavescens

ZHAO Xiaoliang^{1, 2, ,},
MOU He¹,
ZHANG Jing^{1, 2},
ZHANG Weijie^{1, 2, ,}

1.
College of Life Science and Engineering, Lanzhou University of Science and Technology, Lanzhou 730050, China
2.
Key Laboratory of Screening, Evaluation and Deep Processing of Chinese and Tibetan Medicines in Gansu Province, Lanzhou 730050, China

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Received Date: December 26, 2023
Available Online: May 06, 2024

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Abstract

Abstract

Objective: To increase the yield of Sophora flavescens polysaccharides and elucidate their antioxidant and hypolipidemic properties, this study optimised the polysaccharide extraction process through orthogonal experiments, followed by evaluation of their antioxidant and hypolipidemic activities in vitro. Methods: Using polysaccharide yield as the primary metric, this study performed orthogonal experiments to optimise the extraction process, building on preliminary one-way experiments. This study investigated the effect of extraction temperature, number of extractions, material-liquid ratio and extraction time on the polysaccharide yield of Sophora flavescens. Furthermore, this study evaluated the in vitro antioxidant activity of the polysaccharides by investigating their scavenging potential against DPPH radicals, hydroxyl radicals and superoxide anion radicals. In addition, the in vitro hypolipidemic activity of the polysaccharides was evaluated by their binding affinity to sodium cholate (SC), sodium taurocholate (STC), sodium glycylcholate (SGC) and mixed bile salts (MBS). Results: The optimal conditions for the extraction of Sophora flavescens polysaccharide were determined as follows: a temperature of 85 ℃, a material-liquid ratio of 1:35 (g:mL), and an extraction duration of 3.5 hours, yielding an average polysaccharide content of 3.07%±0.07%. The scavenging efficiencies of Sophora flavescens polysaccharides against DPPH radicals, hydroxyl radicals, and superoxide anion radicals at a concentration of 5 mg/mL were measured at 60.1%±3.6%, 39.6%±3.1% and 45.1%±4.1%, respectively. Furthermore, the binding affinities of Sophora flavescens polysaccharides to SC, STC, SGC and MBS at a concentration of 10 mg/mL were determined to be 34.4%±0.75%, 45.4%±0.15%, 19.7%±0.94% and 37.7%±0.64%, respectively. Conclusion: Sophora flavescens polysaccharide exhibits notable in vitro antioxidant and hypolipidemic activities. This investigation furnishes a foundational understanding for the potential development and exploitation of Sophora flavescens polysaccharide.
- Sophora flavescens Ait.,
- polysaccharide,
- process optimized,
- antioxidant activity,
- hypolipidemic activity

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