Application of Recombinase Polymerase Amplification in Detection of Foodborne Pathogen

QIN Xue; FU Shiqian; YANG Xinyan; YANG Tao; GAO Pingpin; DAI Xiaofei; MIAO Chao; MAN Chaoxin; JIANG Yujun

doi:10.13386/j.issn1002-0306.2020100136

Volume 42 Issue 20

Oct. 2021

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Science and Technology of Food Industry > 2021 > 42(20): 449-455. > DOI: 10.13386/j.issn1002-0306.2020100136

QIN Xue, FU Shiqian, YANG Xinyan, et al. Application of Recombinase Polymerase Amplification in Detection of Foodborne Pathogen[J]. Science and Technology of Food Industry, 2021, 42(20): 449−455. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100136.

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Application of Recombinase Polymerase Amplification in Detection of Foodborne Pathogen

Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China

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Received Date: October 19, 2020
Available Online: August 21, 2021

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Abstract

Abstract

Food safety is a major concern for human. Various detection methods for foodborne pathogenic bacteria have become the focus of studies nowadays. Although polymerase chain reaction (PCR), as the most wildly used detection methods for pathogen, has overcome the shortcomings of time-consuming traditional method, the need for accurate temperature control greatly limits its application in field testing. Recombinase polymerase amplification (RPA), as a new isothermal amplification method, has developed rapidly in recent ten years. This method has broken up the barrier of PCR, getting rid of thermal cycle and expensive instruments, which is more suitable for insufficient resources on-site detection. In this manuscript, the mechanism of RPA and the probe design method are provided. Besides, the application of RPA in detecting foodborne pathogenic bacteria is reviewed. The hot topics of RPA development and the prospect of RPA technology development in the future are summarized.
- recombinase polymerase amplification,
- foodborne pathogen,
- rapid detection,
- food safety

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References

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