Extraction, Purification and Antioxidant Activity of Polysaccharide from White Lilacs

WANG Tianyi; ZHANG Qingfen; HUANG Leilei; LIU Jiayi; YANG Fengjian

doi:10.13386/j.issn1002-0306.2023090036

Volume 45 Issue 15

Jul. 2024

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Science and Technology of Food Industry > 2024 > 45(15): 233-243. > DOI: 10.13386/j.issn1002-0306.2023090036

WANG Tianyi, ZHANG Qingfen, HUANG Leilei, et al. Extraction, Purification and Antioxidant Activity of Polysaccharide from White Lilacs[J]. Science and Technology of Food Industry, 2024, 45(15): 233−243. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090036.

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Extraction, Purification and Antioxidant Activity of Polysaccharide from White Lilacs

WANG Tianyi^{1, 2, 3, 4, 5,},
ZHANG Qingfen^{1, 2, 3, 4, 5},
HUANG Leilei^{1, 2, 3, 4, 5},
LIU Jiayi^{1, 2, 3, 4, 5},
YANG Fengjian^{1, 2, 3, 4, 5, ,}

1.
Key Laboratory of Forest Plant Ecology of the Ministry of Education, Northeast Forestry University, Harbin 150040, China
2.
School of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China
3.
Engineering Center of the Ministry of Forestry Biologics Education, Northeast Forestry University, Harbin 150040, China
4.
Key Laboratory of Ecological Utilization of Forest Source Active Substances in Heilongjiang Province, Harbin 150040, China
5.
National and Local Joint Engineering Laboratory for Ecological Utilization of Biological Resources, Northeast Forestry University, Harbin 150040, China

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Received Date: September 05, 2023
Available Online: June 04, 2024

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Abstract

Abstract

Enzyme-assisted ultrasonic extraction was employed to extract polysaccharides from white lilacs. The effects of various experimental conditions on the yield of polysaccharides from white lilacs were systematically analyzed using the response surface methodology (RSM). The crude extract was purified through DEAE-52 cellulose column and Sephadex G-100 gel column chromatography. High-performance gel permeation chromatography (HPGPC), ion chromatography (IC), ultraviolet spectrum analysis, Fourier infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were utilized for characterization of the polysaccharide structure and its antioxidant activity in vitro was investigated. The results demonstrated that the optimal extraction process for polysaccharides from white lilacs involved ultrasonic power 150 W, ultrasonic time 40 min, ultrasonic temperature 40 ℃, enzyme dosage 2.2%, solid-liquid ratio 1:40 g/mL. Under these conditions, the yield of polysaccharides was 3.03%±0.09%. The polysaccharides were purified using a DEAE-52 cellulose column, and the main component SP-c was collected. Subsequently, SP-c-1 was obtained through a Sephadex G-100 gel column. The monosaccharide composition and molar ratio of SP-c-1 were galacturonic acid, arabinose, galactose, rhamnose, glucose, glucuronic acid, xylose=18.39:11.13:8.96:2.61:1:0.83:0.57, respectively. The weight-average molecular weight (Mw) of SP-c-1 was 14069 Da, and the numerical mean molecular weight (Mn) was 13637 Da. SP-c-1 had the characteristic absorption peak of polysaccharide and contained D-grape pyranose configuration. The half-inhibitory concentration (IC₅₀) of SP-c-1 on DPPH and ABTS⁺ free radicals was 0.87 and 1.355 mg/mL, respectively. Overall results indicate that SP-c-1 exhibits significant antioxidant activity.
- white lilacs,
- polysaccharide,
- structural characterization,
- enzyme-assisted ultrasonic extraction,
- antioxidant activity

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