Optimization of Extraction Process of Flavonoids from <i>Fagopyrum esculentum </i>Moench Leaves and Its Antioxidant Properties

Yuedong SONG; Xiaoqing CHEN; Yumin ZHANG; Yanfang ZHANG; Zhiyi WANG; Fei WANG

doi:10.13386/j.issn1002-0306.2020060122

Volume 42 Issue 7

Mar. 2021

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Science and Technology of Food Industry > 2021 > 42(7): 180-187. > DOI: 10.13386/j.issn1002-0306.2020060122

SONG Yuedong, CHEN Xiaoqing, ZHANG Yumin, et al. Optimization of Extraction Process of Flavonoids from Fagopyrum esculentum Moench Leaves and Its Antioxidant Properties[J]. Science and Technology of Food Industry, 2021, 42(7): 180−187. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060122.

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Optimization of Extraction Process of Flavonoids from Fagopyrum esculentum Moench Leaves and Its Antioxidant Properties

School of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo 255049, China

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Received Date: June 09, 2020
Available Online: January 26, 2021

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Abstract

In order to study the extraction process and antioxidant properties of flavonoid in Fagopyrum esculentum Moench leaves. The ultrasonic-assisted extraction process of flavonoids from Fagopyrum esculentum Moench leaves was optimized by response surface method, the contents of flavonoids was analyzed by high performance liquid chromatography (HPLC), and the antioxidant activity was explored.The results showed that when the ultrasonic frequency was 45 kHz, the ultrasonic power was 100 W, the liquid -solid ratio was 30:1(mL:g), the ultrasonic time was 22 min, the ultrasonic temperature was 28 ℃, and the volume fraction of ethanol was 51%, the yield of flavonoids in Fagopyrum esculentum Moench leaves was 80.311mg/g. The relative error was 1.35% compared with the predicted value of 81.414 mg/g, which proved that the theoretical predicted value of the model well fitted with the actual value. The result of HPLC showed that rutin and quercetin were the main components of flavonoid in Fagopyrum esculentum Moench leaves, and their contents were 66.5% and 13.9%, respectively. The antioxidant activity test showed that the half maximal inhibitory concentration (IC₅₀) of the flavonoids to DPPH·, ABTS⁺· and ·OH was 0.012, 0.044, 0.344 mg/mL, respectively, indicated that the flavonoids had strong antioxidant capacity. This experiment provided a theoretical basis for the comprehensive utilization of Fagopyrum esculentum Moench leaves.
- Fagopyrum esculentum Moench leaves,
- flavonoid,
- response surface optimization,
- antioxidation activity

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[1]	唐宇, 邵继荣, 周美亮. 中国荞麦属植物分类学的修订[J]. 植物遗传资源学报,2019,20(3):646−653.
[2]	宋越冬, 王明超, 韩晓静, 等. 超声辅助提取荞麦叶绿原酸及体外降糖活性研究[J]. 食品科技,2020,45(7):242−249.
[3]	Lan-Sook Lee, Eun-Ji Choi, Chang-Hee, et al. Contribution of flavonoids to the antioxidant properties of common and tartary buckwheat[J]. Journal of Cereal Science,2016,68(3):181−186.
[4]	章洁琼, 邹军, 卢扬, 等. 不同荞麦品种主要功能成分分析及评价[J]. 种子,2020,39(2):107−112, 117.
[5]	Zhang W N, Zhu Y Y, Liu Q Q, et al. Identification and quantification of polyphenols in hull, bran and endosperm of common buckwheat (Fagopyrum esculentum) seeds[J]. Journal of Functional Foods,2017,38(11):363−369.
[6]	Tomotake H, Kayashita J, Kato N. Hypolipidemic activity of common (Fagopyum esculentum Moench) and tartary (Fagopyum esculentum Gaertn.) buckwheat[J]. Jonrnal of the Science of Food & Agriculture,2015,95(10):1963−1967.
[7]	林红梅, 韩淑英, 栾新段, 等. 甜荞麦种子提取物镇痛抗炎作用的实验研究[J]. 华北煤炭医学院学报,2003(3):290−291.
[8]	Nguyen Ngoc Thanh Tien, Le Ngoc Dang Trinh, Naoto Inoue, et al. Nutritional composition, bioactive compounds, and diabetic enzyme inhibition capacity of three varieties of buckwheat in Japan[J]. Cereal Chemistry,2018,95(5):615−624. doi: 10.1002/cche.10069
[9]	Mariotti M, Andreuccetti V, Nuvoloni R, et al. Rutin and quercetin content in the forage of common buckwheat as affected by maturity and conservation method[J]. Grassland Science,2017,63(3):169−176. doi: 10.1111/grs.12160
[10]	符献琼, 张慧, 李承业, 等. 荞麦叶的营养成分及其营养特性[J]. 新疆农业科学,1994(3):105−108.
[11]	孙艳, 崔旭盛, 刘静, 等. 酸枣叶黄酮的提取工艺优化及其抗秀丽隐杆线虫氧化损伤活性[J]. 食品工业科技,2020,41(8):143−150.
[12]	王树宁, 宋照军, 黄滢洁, 等. 响应面法优化超声波辅助提取侧柏叶总黄酮工艺[J]. 食品研究与开发,2020,41(9):88−93. doi: 10.12161/j.issn.1005-6521.2020.09.014
[13]	Wang Y Y, Duan X, Ren G Y, et al. Comparative study on the flavonoids extraction rate and antioxidant activity of onions treated by three different drying methods[J]. Drying Technology,2018,24(11):245−252.
[14]	Wang Y Q, Gao Y J, Ding H, et al. Subcritical ethanol extraction of flavonoids from Moringa oleifera leaf and evaluation of antioxidant activity[J]. Food Chemistry,2017,218:152−158. doi: 10.1016/j.foodchem.2016.09.058
[15]	Li J K, Wu C C, Li F, et al. Optimization of ultrasound-assisted water extraction of flavonoids from Psidium guajava leaves by response surface analysis[J]. Preparative Biochemistry and Biotechnology,2019,49(1):21−29. doi: 10.1080/10826068.2018.1466158
[16]	徐树来, 王丽, 任红波, 等. 蒲公英黄酮的提取工艺优化及主要成分浅析[J]. 食品工业科技,2020,41(19):172−178.
[17]	魏永生, 王永宁, 石玉平, 等. 分光光度法测定总黄酮含量的实验条件研究[J]. 青海大学学报(自然科学版),2003,21(3):61−63. doi: 10.3969/j.issn.1006-8996.2003.03.019
[18]	Song H F,Zhang Q B, Zhang A S, et al. In vitro antioxidant activity of polysaccharides extracted from Bryopsis plumosa[J]. Carbohydrate Polymers,2010,80(4):1057−1061. doi: 10.1016/j.carbpol.2010.01.024
[19]	] Re R, Pellegrini N, Proteggente A, et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay[J]. Free Radical Biology and Medicine,1999,26(9):1231−1237.
[20]	董迪迪, 王鸿飞, 周增群, 等. 杨梅籽油抗氧化活性及其调节血脂作用的研究[J]. 中国粮油学报,2014,29(5):53−57.
[21]	Mukhopadhyay D, Dasgupta P, Roy D S, et al. A sensitive in vitro spectrophotometric hydrogen peroxide scavenging assay using 1, 10-phenanthroline[J]. Free Radicals and Antioxidants,2016,6(1):124−132. doi: 10.5530/fra.2016.1.15
[22]	宋伟. 银杏叶超声萃取机制及提取条件的研究[D]. 哈尔滨: 哈尔滨工业大学, 2009.
[23]	徐艳丽, 赵文英, 孙彦妮, 等. 响应面优化苦参种子黄酮超声提取工艺[J]. 粮食与油脂,2019,32(2):87−90. doi: 10.3969/j.issn.1008-9578.2019.02.025
[24]	陈建福. 响应面优化黄槿叶总黄酮提取工艺及其抗氧化活性[J]. 食品研究与开发,2019,40(14):85−91.
[25]	禄璐, 米佳, 罗青, 等. 枸杞总黄酮提取工艺优化及其体外抗氧化活性分析[J]. 食品工业科技,2019,40(24):165−171.
[26]	秦晶晶, 钱慧琴, 赵媛, 等. 柿叶总黄酮提取工艺优化及其抗氧化活性[J]. 食品工业科技,2020,41(13):32−38, 45.
[27]	田明杰, 谭宏渊, 叶帆宇, 等. 福白菊总黄酮的微波辅助提取工艺优化及其抗氧化活性研究[J]. 中国酿造,2020,39(1):170−174. doi: 10.11882/j.issn.0254-5071.2020.01.033
[28]	孙艳华, 刘永彬. 乙醇预处理法提取荞麦茎叶中黄酮类化合物的研究[J]. 天津医科大学学报,2006(3):375−377, 387. doi: 10.3969/j.issn.1006-8147.2006.03.005
[29]	单科开, 王鸿飞, 许凤, 等. 苦菜总黄酮超声波辅助提取及抗氧化能力研究[J]. 核农学报,2019,33(9):1755−1764. doi: 10.11869/j.issn.100-8551.2019.09.1755

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