Preparation of Selenium-enriched <i>Morchella</i> Polysaccharide and Its Antitumor Activity <i>in Vitro</i>

LI Beibei; WANG Huiqin; SUN Yizheng

doi:10.13386/j.issn1002-0306.2023080337

Volume 45 Issue 15

Jul. 2024

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Science and Technology of Food Industry > 2024 > 45(15): 213-222. > DOI: 10.13386/j.issn1002-0306.2023080337

LI Beibei, WANG Huiqin, SUN Yizheng. Preparation of Selenium-enriched Morchella Polysaccharide and Its Antitumor Activity in Vitro[J]. Science and Technology of Food Industry, 2024, 45(15): 213−222. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023080337.

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Preparation of Selenium-enriched Morchella Polysaccharide and Its Antitumor Activity in Vitro

Zhengzhou Shuqing Medical College, Zhengzhou 450064, China

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Received Date: September 03, 2023
Available Online: June 03, 2024

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Abstract

Abstract

This study aimed to optimize the extraction and purification processes for selenium-enriched Morchella esculenta (L.) Pers. (also known as Morchella) polysaccharides (Se-MPS) and to evaluate their anti-tumor activities and mechanisms. The extraction process was optimized using single-factor and response surface methodology. The crude polysaccharides were purified through column chromatography and their chemical composition was analyzed. The anti-tumor activity of Se-MPS was evaluated using HepG2 cells through a series of assays, including CCK-8, colony formation, scratching, and Transwell assays. The potential anti-tumor mechanisms of Se-MPS were assessed using RT-qPCR, Western blot, and immunofluorescence methods. Experimental results from single-factor and response surface methodology revealed that the optimal extraction conditions for selenium-enriched Morchella crude polysaccharides were: An extraction time of 2.5 h, a liquid-to-material ratio of 45:1 (mL/g), and an extraction temperature of 82 ℃, yielding a crude polysaccharides yield of 7.95%. After purification, the total sugar content of Se-MPS reached 95.86%, with a selenium concentration of 76.07±0.32 mg/g. Furthermore, Se-MPS significantly reduced colony formation efficiency, cell migration rate, and the number of invasive cells in HepG2 cells (P<0.05). Meanwhile, Se-MPS inhibited the PI3K/Akt signaling pathway, down-regulated mRNA expression of glucose transporter 2 (GLUT2), and reduced glucose uptake in cells. The results indicated that optimizing extraction time, liquid-to-material ratio, and extraction temperature could improve the yield of selenium-enriched Morchella crude polysaccharides. Additionally, Se-MPS exhibited strong anti-tumor activity by regulating the PI3K/Akt pathway. This study provides evidence of the anti-tumor activities of Se-MPS and offers insights for the development of related functional foods.
- selenium,
- Morchella polysaccharide,
- chemical composition,
- response surface optimization,
- anti-tumor activity

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