Dynamic Changes and Structural Analysis of the Bacterial Community in Paocai Brine

LI Jingchao; DENG Wei; SHE Rong; YANG Xiaoyan; XIAO Wen

doi:10.13386/j.issn1002-0306.2021120200

Volume 43 Issue 20

Oct. 2022

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Science and Technology of Food Industry > 2022 > 43(20): 144-151. > DOI: 10.13386/j.issn1002-0306.2021120200

LI Jingchao, DENG Wei, SHE Rong, et al. Dynamic Changes and Structural Analysis of the Bacterial Community in Paocai Brine[J]. Science and Technology of Food Industry, 2022, 43(20): 144−151. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021120200.

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Dynamic Changes and Structural Analysis of the Bacterial Community in Paocai Brine

LI Jingchao^1,,
DENG Wei¹,
SHE Rong^{1, 2, 3},
YANG Xiaoyan^{1, 2, 3, ,},
XIAO Wen^{1, 2, 3}

1.
Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali 671003, China
2.
Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China, Dali 671003, China
3.
The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region from Dali University, Dali 671003, China

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Received Date: December 19, 2021
Available Online: July 28, 2022

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Abstract

Abstract

Bacteria are the key group of micro-organisms during the fermentation of Paocai. This study applied the high-throughput sequencing technology to trace the dynamics of Paocai brine bacterial community structures at constant temperature (25 ℃) under airtight microcosm condition. The results showed that there were 10 phyla and 205 genera had been detected within 30 days after the experiment setup. Among them, there were 12 dominant genera which accounted for 94.7%~97.8% of the total OTUs. The sub-dominant were 13 genera and accounted for 2.1%~3.3%. The rare genera were 180 genera and accounted for 0.08%~2.7%. During the 1~7 days after the experiment setup, the community was relatively stable (phase A). From the 8~20 days, the sub-dominant and rare genera were continuously changing (phase B), and on the 8th day, the communities of sub-dominant and rare genera were acutely fluctuated. From 22~30 days, all of the genera changed obviously (phase C). Meanwhile, the Chao1, Ace indices were increasing during 1~30 days, in addition, the Shannon and Simpson indices reached the thresholds during phase C. Therefore, phase A and B, phase B and C had the similar communities, but not the phase A and C, while the pH during the three phases changed correspondingly. The results also showed that at 25 ℃, the dynamics of sub-dominant and rare genera were the key factor which caused the dynamic of the bacterial communities in Paocai brine during the 30 days experiment. In addition, the accumulated effect had been detected, the pH and the bacterial communities havd mutual influences. The optimal preservation time for the Paocai brine was one week under airtight condition at 25 ℃. The functional effects of rare genera from Paocai brine need pay more attentions on.
- Paocai brine,
- high-throughput sequencing,
- food function,
- rare taxa,
- community structure

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References (31)

References

[1]	陈功, 夏有书, 张其圣, 等. 从中国泡菜看四川泡菜及泡菜坛[J]. 中国酿造,2010,29(8):5−8. [CHEN G, XIA Y S, ZHANG Q S, et al. Discussion of Sichuan pickles and pickle jars from the view of Chinese pickles[J]. China Brewing,2010,29(8):5−8. doi: 10.3969/j.issn.0254-5071.2010.08.002
[2]	JUNG K O, PARK K Y, BULLERMAN L B. Anticancer effects of leek kimchi on human cancer cells[J]. Preventive Nutrition & Food Science,2002,7(7):250−254.
[3]	凌洁玉, 龚文秀, 包梦莹, 等. 泡菜中乳酸菌的分离鉴定及其抗氧化能力的比较研究[J]. 中国调味品,2015,40(7):32−36. [LING J Y, GONG W X, BAO M Y, et al. Comparative study on isolation and identification of lactic acid bacteria from pickle and their antioxidant activity[J]. China Condiment,2015,40(7):32−36. doi: 10.3969/j.issn.1000-9973.2015.07.006
[4]	DING W, SHI C, CHEN M, et al. Screening for lactic acid bacteria in traditional fermented Tibetan yak milk and evaluating their probiotic and cholesterol lowering potentials in rats fed a high-cholesterol diet[J]. Journal of Functional Foods,2017,32:324−332.
[5]	KIM E K, AN S Y, LEE M S, et al. Fermented kimchi reduces body weight and improves metabolic parameters in overweight and obese patients[J]. Nutrition Research,2011,31(6):436−443.
[6]	张锡茹, 关慧, 邢少华, 等. 泡菜微生物演替与风味物质变化的研究进展[J/OL]. 食品科学, 2021: 1−17. http://kns.cnki.net/kcms/detail/11.2206.TS.20210308.1728.022.html. ZHANG X R, GUAN H, XING S H, et al. Advances in research on microbial succession and flavor of Paocai [J/OL]. Food Science, 2021: 1−17. http://kns.cnki.net/kcms/detail/11.2206.TS.20210308.1728.022.html.
[7]	李恒, 陈功, 伍亚龙, 等. 高通量测序方法研究传统四川泡菜母水中微生物群落的动态变化[J]. 食品科学,2018,39(24):8. [LI H, CHEN G, WU Y L, et al. Analysis of microbial community dynamics of traditional Sichuan paocai brine by high-throughput sequencing[J]. Food Science,2018,39(24):8. doi: 10.7506/spkx1002-6630-201824020
[8]	LIANG H, ZHANG A, WU Z, et al. Microbial community characteristics in industrial matured Chinese Paocai, a fermented vegetable food, from different factories[J]. Food Science & Technology Research,2016,22(5):595−604.
[9]	张其圣, 陈功, 申文熹, 等. 中国泡菜乳酸菌群落结构动态变化研究进展[J]. 食品与发酵科技,2016,52(6):1−8. [ZHANG Q S, CHEN G, SHEN W X, et al. Review of the diversity and dynamics of lactic acid bacteria in Chinese Paocai[J]. Food and Fermentation Science & Technology,2016,52(6):1−8.
[10]	徐丹萍, 蒲彪, 刘书亮, 等. 不同发酵方式的泡菜挥发性成分分析[J]. 食品科学,2015,36(16):94−100. [XU D P, PU B, LIU S L, et al. Analysis of volatile components in pickles fermented with different starter cultures[J]. Food Science,2015,36(16):94−100. doi: 10.7506/spkx1002-6630-201516017
[11]	卢沿钢, 董全. 中、日、韩三国泡菜加工工艺的对比[J]. 食品与发酵科技,2011,47(4):5−9. [LU Y G, DONG Q. The comparison of Chinese pickle, Japanese pickle and kimchi processing technology[J]. Food and Fermentation Technology,2011,47(4):5−9. doi: 10.3969/j.issn.1674-506X.2011.04.002
[12]	李文婷, 车振明. 泡菜中亚硝酸盐安全性研究新进展[J]. 中国调味品,2011,36(7):1−3, 17. [LI W T, CHE Z M. Overview the new progresses of nitrite safety in pickles[J]. China Condiment,2011,36(7):1−3, 17. doi: 10.3969/j.issn.1000-9973.2011.07.001
[13]	张杰, 赵志峰, 王佐军, 等. 四川泡菜菌相构成、风味与质量安全性研究进展[J]. 中国调味品,2021,46(10):178−182,197. [ZHANG J, ZHAO Z F, WANG Z J, et al. Research progress on microflora composition, flavor and quality safety of Sichuan pickles[J]. China Condiment,2021,46(10):178−182,197. doi: 10.3969/j.issn.1000-9973.2021.10.035
[14]	李书华, 蒲彪, 陈封政. 泡菜的功能及防腐研究进展[J]. 中国酿造,2005,24(4):6−8. [LI S H, PU B, CHEN F Z. Research progress of function and shelf~life of pickles[J]. China Brewing,2005,24(4):6−8. doi: 10.3969/j.issn.0254-5071.2005.04.002
[15]	刘苏萌, 王丽娟, 何培新. 泡菜中具有胆固醇降解功能益生菌的筛选研究[J]. 粮食与油脂,2016,29(1):72−74. [LIU S M, WANG L J, HE P X. Research on screening of cholesterol reducing probiotics from pickle vegetable[J]. Cereals & Oils,2016,29(1):72−74. doi: 10.3969/j.issn.1008-9578.2016.01.020
[16]	姚粟, 王鹏辉, 白飞荣, 等. 中国传统发酵食品用微生物菌种名单研究（第二版）[J/OL]. 食品与发酵工业, 2021: 1−30. https://doi.org/10.13995/j.cnki.11~1802/ts.029332. YAO L, WANG P H, BAI F R, et al. Research on the inventory of microbial food cultures in Chinese traditional fermented foods (2nd edition)[J/OL]. Food and Fermentation Industries, 2021: 1−30. https://doi.org/10.13995/j.cnki.11~1802/ts.029332.
[17]	RAPPÉ M S, GIOVANNONI S J. The uncultured microbial majority[J]. Annual Reviews in Microbiology,2003,57(1):369−394.
[18]	佟婷婷, 田丰伟, 王刚, 等. 基于宏基因组分析四川泡菜母水作引子的泡菜发酵过程中细菌多样性变化[J]. 食品工业科技,2015,36(21):173−177. [TONG T T, TIAN F W, WANG G. Metagenomic analysis of bacterial diversity changes during vegetables fermentation using Sichuan pickle water as starter[J]. Science and Technology of Food Industry,2015,36(21):173−177.
[19]	邓维琴, 伍亚龙, 陈功, 等. 多代发酵泡菜母水微生物多样性及其理化指标动态研究[J]. 食品与发酵工业,2018,44(8):49−56. [DENG W Q, WU Y L, CHEN G, et al. Study on microbial diversity and physiochemical index of multigenerational fermented Paocai brine[J]. Food and Fermentation Industries,2018,44(8):49−56.
[20]	CALLAHAN B J, MCMURDIE P J, ROSEN M J, et al. DADA2: High-resolution sample inference from Illumina amplicon data[J]. Nature Methods,2016,13(7):581−583.
[21]	BOKULICH N A, KAEHLER B D, RAM R J, et al. Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2's q2-feature-classifier plugin[J]. Microbiome,2018,6(1):1−17.
[22]	侯建伟, 邢存芳, 邓晓梅, 等. pH对花椒根区土壤细菌群落结构的影响[J]. 西北农林科技大学学报(自然科学版),2020,48(5):8. [HOU J W, XING C F, DENG X M, et al. Effect of pH on soil bacterial community structure in root zone of prickly ash[J]. Journal of Northwest A& F University (Natural Science Edition),2020,48(5):8.
[23]	陈功, 张其圣, 李恒, 等. 中国泡菜发酵态相对稳定性的研究及应用[J]. 食品与发酵科技,2020,56(1):54−63. [CHEN G, ZHANG Q S, LI S, et al. Research and application of the stable fermentation stage of Chinese paocai[J]. Food and Fermentation Sciences & Technology,2020,56(1):54−63. doi: 10.3969/j.issn.1674-506X.2020.01.011
[24]	JIAO S, LU Y. Soil pH and temperature regulate assembly processes of abundant and rare bacterial communities in agricultural ecosystems[J]. Environmental Microbiology,2020,22(3):1052−1065.
[25]	PESTER M, BITTNER N, DEEVONG P, et al. A ‘rare biosphere’ microorganism contributes to sulfate reduction in a peatland[J]. The ISME Journal,2010,4(12):1591−1602.
[26]	SAURET C, SÉVERIN T, VÉTION G, et al. ‘Rare biosphere’ bacteria as key phenanthrene degraders in coastal seawaters[J]. Environmental Pollution,2014,194(11):246−253.
[27]	ELSHAHED M S, YOUSSEF N H, SPAIN A M, et al. Novelty and uniqueness patterns of rare members of the soil biosphere[J]. Applied and Environmental Microbiology,2008,74(17):5422−5428.
[28]	ZHOU Q, ZHANG X, HE R, et al. The The composition and assembly of bacterial communities across the rhizosphere and phyllosphere compartments of Phragmites australis[J]. Diversity,2019,11(6):98.
[29]	LYNCH M, NEUFELD J D. Ecology and exploration of the rare biosphere[J]. Nature Reviews Microbiology,2015,13(4):217−229.
[30]	YACHI, SHIGEO, LOREAU, et al. Biodiversity and ecosystem productivity in a fluctuating environment: The insurance hypothesis[J]. Proceedings of the National Academy of Sciences of the United States of America,1999,96(4):1463−1468.
[31]	JONES S E, KARL L D. Dormancy contributes to the maintenance of microbial diversity[J]. Proceedings of the National Academy of Sciences,2010,107(13):5881−5886.

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