The Improvement of <i>Lactobacillus plantarum</i> Fermented Milk in Characterization and Anti-<i>Bacillus cereus</i> Activity by Galactooligosacchari

LIU Le; ZOU Kaixiang; SHAO Kaisheng; YU Hui; TAO Xueying; WEI Hua; ZHANG Zhihong

doi:10.13386/j.issn1002-0306.2021110013

Volume 43 Issue 15

Jul. 2022

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Science and Technology of Food Industry > 2022 > 43(15): 139-147. > DOI: 10.13386/j.issn1002-0306.2021110013

LIU Le, ZOU Kaixiang, SHAO Kaisheng, et al. The Improvement of Lactobacillus plantarum Fermented Milk in Characterization and Anti-Bacillus cereus Activity by Galactooligosacchari[J]. Science and Technology of Food Industry, 2022, 43(15): 139−147. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110013.

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The Improvement of Lactobacillus plantarum Fermented Milk in Characterization and Anti-Bacillus cereus Activity by Galactooligosacchari

LIU Le^1,,
ZOU Kaixiang¹,
SHAO Kaisheng¹,
YU Hui²,
TAO Xueying¹,
WEI Hua^{1, 3},
ZHANG Zhihong^{1, 3, ,}

1.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
2.
Jiangxi Provincial Center for Disease Control and Prevention, Nanchang 330029, China
3.
Jiangxi-OAI Joint Research Institute, Nanchang Univerisity, Nanchang 330047, China

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Received Date: November 02, 2021
Available Online: June 02, 2022

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Abstract

Abstract

In order to test the effect of galactooligosaccharide (GOS) on the characterization and antibacterial activity of milk fermented by Lactobacillus plantarum, the main factors affecting the fermented milk were investigated and the most suitable fermentation conditions for improving the characterization of fermented milk were obtained with response surface analysis. The enterotoxigenic Bacillus cereus HN001 was used as an indicator to study the effect of GOS addition on the antibacterial activity of L. plantarum ZDY2013 fermented milk. The results showed that L. plantarum could use GOS for metabolism in vitro and inhibit the growth of B. cereus effectively. The proper addition of GOS in milk could increase the number of viable bacteria, reduce the pH and enhance the water holding capacity of L. plantarum fermented milk. The result from response surface analysis was found that the best fermentation conditions for fermented milk were 2.0% inoculums of L. plantarum, 1.0% GOS, fermented at 42 ℃ for 24 h. Additionally, GOS addition in fermented milk could effectively control the concentration of enterotoxigenic B. cereus HN001 to below 10⁶ CFU/mL. The results of this study provide the foundation for the application of GOS and L. plantarum ZDY2013 in fermented milk.
- galactooligosaccharides,
- Lactobacillus plantarum,
- response surface analysis,
- fermented milk,
- antibacterial activity,
- Bacillus cereus

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References (29)

References

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