Study on Mutation Breeding of High-Yield Bacteriocin <i>Lactobacillus plantarum</i> by Heavy Ion Beam Irradiation

MA Heping; PENG Zhangpu; ZHANG Wenqi; LIU Caiyun; WANG Jie; WANG Shuyang; SHAO Jianning

doi:10.13386/j.issn1002-0306.2021030234

Volume 42 Issue 15

Jul. 2021

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Science and Technology of Food Industry > 2021 > 42(15): 139-143. > DOI: 10.13386/j.issn1002-0306.2021030234

MA Heping, PENG Zhangpu, ZHANG Wenqi, et al. Study on Mutation Breeding of High-Yield Bacteriocin Lactobacillus plantarum by Heavy Ion Beam Irradiation[J]. Science and Technology of Food Industry, 2021, 42(15): 139−143. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030234.

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Study on Mutation Breeding of High-Yield Bacteriocin Lactobacillus plantarum by Heavy Ion Beam Irradiation

1.
Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province, Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, China
2.
Gansu Academy of Sciences, Lanzhou 730000, China
3.
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China

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Received Date: March 18, 2021
Available Online: May 31, 2021

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Abstract

Abstract

The heavy ion beam of ¹²C⁶⁺ was used to irradiate the original strain Lactobacillus plantarum Lp1 of bacteriocin-producing. The lethality and mutation rate of theoriginal strain Lp1 at different irradiation doses were analyzed and compared, and the mutant strains with high bacteriocin production were screened by double layer agar diffusion method. At the irradiation dose of 300 Gy, the lethality rate and positive mutation rate of the original strain Lp1 were 86.3% and 28.28%, respectively.In the 300 Gy irradiation bacterial suspension, mutant strains Lp092 and Lp085 with high bacteriocin production were isolated and screened, the diameters of inhibition zones of Escherichia coli as the indicator bacteria were increased by 20.64%, 19.36%, the diameters of inhibition zones of staphylococcus aureus as the indicator bacteria were increased by 17.16%, 14.14% than the original strain Lp1, respectively.After 5 times successive generations, 2 strains Lp092 and Lp085 have good genetic stability for bacteriocin production.
- Lactobacillus plantarum,
- bacteriocin,
- heavy ion beam,
- irradiation,
- mutation breeding

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References

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