Study on Optimization of Ultrasonic-Assisted Extraction Technology of Total Flavonoids from <i>Kadsura coccinea</i> Flowers and Its Antioxidant Activity

LI Yajun; YI Que; YANG Junheng; DENG Xiaomei; LIANG Zhonghou

doi:10.13386/j.issn1002-0306.2020090267

Volume 42 Issue 13

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(13): 179-183. > DOI: 10.13386/j.issn1002-0306.2020090267

LI Yajun, YI Que, YANG Junheng, et al. Study on Optimization of Ultrasonic-Assisted Extraction Technology of Total Flavonoids from Kadsura coccinea Flowers and Its Antioxidant Activity [J]. Science and Technology of Food Industry, 2021, 42(13): 179−183. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090267.

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Study on Optimization of Ultrasonic-Assisted Extraction Technology of Total Flavonoids from Kadsura coccinea Flowers and Its Antioxidant Activity

1.
College of Medicine & Technology, Hunan Polytechnic of Environment and Biology, Hengyang 421005, China
2.
Landscape College, Hunan Polytechnic of Environment and Biology, Hengyang 421005, China

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Received Date: September 26, 2020
Available Online: May 10, 2021

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Abstract

Abstract

Objective: To optimize the extraction technology and study the antioxidant activity of total flavonoids from the Kadsura coccinea flowers. Methods: The optimum extraction process of total flavonoids from the Kadsura coccinea flowers was optimized by single factor test (ethanol concentration, ultrasonic time, solid-liquid ratio, ultrasonic temperature) and orthogonal experiment. The scavenging ability of total flavonoids on ABTS and DPPH free radicals was evaluated. Results: Full opening period(June), ultrasonic time 45 min, solid-liquid ratio 1:30 mg/mL, ultrasonic temperature 60 ℃, ethanol concentration 85% were the optimal extraction conditions, the extraction quantity of optimum extraction condition was 19.25 mg/g. When the concentration of total flavonoids was 0.8 mg/mL, the scavenging rate on DPPH free radical was 82.1%, it was 87.9% of that of vitamin C. When the concentration of total flavonoids was 0.4 m, the scavenging ability on ABTS free radical was similar to that of vitamin C. The IC₅₀of total flavonoids from the Kadsura coccinea flowers against DPPH free radical and ABTS radical were 0.13, 0.046 mg/mL, respectively.Conclusion: The extraction method is feasible and the extraction conditions are reliable, the total flavonoids of Kadsura coccinea flowers can be used as a source of natural antioxidants.
- Kadsura coccinea flowers,
- total flavonoids,
- process optimization,
- ultrasonic-assisted extraction,
- antioxidant activity

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