Study on Microbial Diversity in Milk of Yak and Cattle-Yak in Qinghai-Tibet Plateau Based on High-throughput Sequencing Technology

Jing MA; Linlin ZHANG; Shatuo CHAI; Xun WANG; Zhanhong CUI; Lu SUN; Shujie LIU

doi:10.13386/j.issn1002-0306.2020080045

Volume 42 Issue 9

Apr. 2021

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Science and Technology of Food Industry > 2021 > 42(9): 122-128. > DOI: 10.13386/j.issn1002-0306.2020080045

MA Jing, ZHANG Linlin, CHAI Shatuo, et al. Study on Microbial Diversity in Milk of Yak and Cattle-Yak in Qinghai-Tibet Plateau Based on High-throughput Sequencing Technology[J]. Science and Technology of Food Industry, 2021, 42(9): 122−128. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080045.

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Study on Microbial Diversity in Milk of Yak and Cattle-Yak in Qinghai-Tibet Plateau Based on High-throughput Sequencing Technology

Jing MA^{1, 2, 3,},
Linlin ZHANG^{1, 2, 3},
Shatuo CHAI^{1, 2, 3},
Xun WANG^{1, 2, 3},
Zhanhong CUI^{1, 2, 3},
Lu SUN^{1, 2, 3, ,},
Shujie LIU^{1, 2, 3, ,}

1.
Qinghai Academy of Science and Veterinary Medicine of Qinghai University, Xining 810016, China
2.
Qinghai Plateau Yak Research Center, Xining 810016, China
3.
Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Xining 810016, China

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Received Date: August 15, 2020
Available Online: March 15, 2021

Graphical Abstract

Abstract

Abstract

This study aimed to explore the composition and diversity of microorganisms in raw milk of yak and cattle-yak. The 16S rDNA V4 region of those two kinds of raw milk was sequencing by high-throughput technology and comparative analysis of bioinformatics.The results showed that a total of 5516 OTUs were obtained with 97% similarity level.The comparison of microbial phylum levels shows that Proteobacteria, Firmicutes and Actinobacteria were the common dominant bacteria phylum for yak milk and cattle-yak milk.The relative abundances of the three Microbes in the yak milk were 29.80%, 35.99% and 8.41%, respectively. The relative abundances of the three microbes in the cattle-yakmilk were 45.36%, 25.79% and 7.39%, respectively. At the genus level, the dominant genus of yak milk was unidentified-Cyanobacteria, and the relative abundance was 9.32%. The dominant genus of cattle-yak milk was Bradyrhizobium, which accounts for 11.53%. At the species level, Bradyrhizobium elkanii and Kosakonia oryzae were the dominant strains in milk of yak and cattle-yak. The two strains accounted for 2.42% and 3.36% in yak milk, respectively.And the two types of bacteria account for 11.53% and 5.53% of the milk in the cattle-yak, respectively. Alpha diversity analysis shows that there are significant differences between different milk richness estimates (P<0.05).And the richness and diversity ofcattle-yak milk was higher.
- yak milk,
- yak-cattlle milk,
- microbial diversity,
- 16S rDNA

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References (31)

References

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