Effect of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> Bi66 on Alleviating Constipation and Regulating Gut Microbiota

CHEN Zhixian; PENG Ning; ZHANG Yan

doi:10.13386/j.issn1002-0306.2023090193

Volume 46 Issue 7

Mar. 2025

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Science and Technology of Food Industry > 2025 > 46(7): 308-314. > DOI: 10.13386/j.issn1002-0306.2023090193

CHEN Zhixian, PENG Ning, ZHANG Yan. Effect of Bifidobacterium animalis subsp. lactis Bi66 on Alleviating Constipation and Regulating Gut Microbiota[J]. Science and Technology of Food Industry, 2025, 46(7): 308−314. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090193.

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Effect of Bifidobacterium animalis subsp. lactis Bi66 on Alleviating Constipation and Regulating Gut Microbiota

CHEN Zhixian^{1, 2, 3,},
PENG Ning^{1, 2, 3},
ZHANG Yan^{1, 2, 3, ,}

1.
National Key Laboratory of Agricultural Microbiology, Yichang 443003, China
2.
The Hubei Provincial Key Laboratory of Yeast Function, Yichang 443003, China
3.
Yichang Engineering and Technology Research Center of Nutrition and Health Food, Yichang 443003, China

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Received Date: September 19, 2023
Available Online: February 07, 2025

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Abstract

Abstract

Objective: The objective of this study was to evaluate the efficacy of Bifidobacterium animalis subsp. lactis Bi66 in alleviating constipation in mice and exploring its mechanism, thereby providing a high-quality strain for native culture resources. Methods: Male Balb/c mice were randomly divided into the control group, the constipation model group, the Bi66 group, and the BB12 group. Probiotics were orally administered to the mice for 14 days followed by loperamide administration to induce constipation. Fecal indexes, intestinal propulsive rate, and serum gastrointestinal regulatory peptides of mice were measured, and gut microbiota was analyzed by 16S rRNA gene sequencing. Result: Bifidobacterium animalis subsp. lactis Bi66 significantly increased the number of feces particles, fecal wet weight, and fecal water content (P<0.05). It also significantly reduced the time of first black stool (P<0.05) and improved the intestinal propulsive rate in constipated mice. Moreover, Bi66 significantly improved motilin (P<0.05), gastrin (P<0.01), and decreased vasoactive intestinal peptide and endothelin levels (P<0.05). The analysis of gut microbiota revealed that the administration of Bifidobacterium animalis subsp. lactis Bi66 enhanced both the abundance and diversity of intestinal flora in constipated mice. Specifically, Bi66 exhibited a remarkable reduction in Firmicutes while promoting an increase in Bacteroidetes in constipated mice. At the genus level, the relative abundances of Alistipes, Roseburia, Ligilactobacillus, Blautia, unclassified-Muribaculaceae, and Alloprevotella showed significant increase (P<0.05, P<0.01). Conclusion: Bifidobacterium animalis subsp. lactis Bi66 alleviated constipation in mice by regulating gastrointestinal regulatory peptides and gut microbiota.
- Bifidobacterium animalis subsp. lactis Bi66,
- constipation,
- gut microbiota,
- gastrointestinal regulatory peptides

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References (31)

References

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