Effect to Intestinal Microbiota Regulation and Laxative Function to <i>Lactobacillus</i> <i>paracasei</i>

ZUO Yifan; XIAO Meng; SU Xiangping; ZOU Kun; LIU Lingyan; TAN Yali; LI Xiao; YANG Jing; ZHENG Zejiang

doi:10.13386/j.issn1002-0306.2023090175

Volume 45 Issue 21

Oct. 2024

Turn off MathJax

Article Contents

Abstract

References

Supplements

Science and Technology of Food Industry > 2024 > 45(21): 329-337. > DOI: 10.13386/j.issn1002-0306.2023090175

ZUO Yifan, XIAO Meng, SU Xiangping, et al. Effect to Intestinal Microbiota Regulation and Laxative Function to Lactobacillus paracasei[J]. Science and Technology of Food Industry, 2024, 45(21): 329−337. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023090175.

Citation:

PDF (1839 KB)

Effect to Intestinal Microbiota Regulation and Laxative Function to Lactobacillus paracasei

ZUO Yifan^1,,
XIAO Meng¹,
SU Xiangping^{1, 2, 3, ,},
ZOU Kun^{1, 2},
LIU Lingyan⁴,
TAN Yali⁴,
LI Xiao⁴,
YANG Jing⁵,
ZHENG Zejiang¹

1.
Hubei Key Laboratory of Natural Products Research and Development, College of Biological & Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, China
2.
Hubei Biological Enzyme Engineering Technology Research Center, China Three Gorges University, Yichang 443002, China
3.
Hangzhou Zhongce Vocational School Qiantang, Hangzhou 310020, China
4.
Angel Biotechnology Co., Ltd., Yichang 443003, China
5.
Third-grade Pharmacological Laboratory on Traditional Chinese Medicine, State Administration of Traditional Chinese Medicine, China Three Gorges University; College of Medicine and Health Sciences, China Three Gorges University, Yichang 443002, China

More Information

Received Date: September 17, 2023
Available Online: August 26, 2024

Graphical Abstract

Abstract

Abstract

The effects of five strains of Lactobacillus paracasei from Tibet on constipation and gut microbiota of mice were studied. One hundred and sixty male Kunming mice were randomly divided into two groups: A and B. Each group was randomly divided into a blank subgroup, a model subgroup, five experimental strain (including T1-5, T1-7, T1-9, T1-d, and 5B-1) subgroup and a control strain 6108 subgroup for the laxative function test, with 10 mice in each subgroup. Among them, 80 mice in group A were used to measure the small intestine propulsion rate of mice, and 80 mice in group B were used to measure defecation status. Strains T1-5, T1-9, and 5B-1 with good performance in laxative function test were then selected to detect the regulation of intestinal microbiota and total 24 male BALB/c mice were selected for this experiment, and were divided into a blank subgroup, a T1-5 subgroup, T1-9 subgroup, and 5B-1 subgroup, with 6 mice in each subgroup. The experimental results of the laxative function test showed that: Compared with the model group, the small intestine propulsion rate of mice in T1-9 and 5B-1 groups was significantly higher than that of the model group (P<0.01). The first red stool time was significantly shortened in the T1-9 and 5B-1 groups (P<0.01). The number of fecal particles in the T1-9 group, 5B-1 group, and control strain 6108 group increased significantly at 6 h (P<0.05). The wet weight of feces in the T1-9 and 6108 groups increased significantly (P<0.01, P<0.05). The experimental results of the microbial community sequencing test showed that: compared with the blank group, the abundance of probiotics in the three strain groups T1-5, T1-9, and 5B-1 Lactobacillaceae and Rikenellaceae was significantly increased, including Prevotellaceae in the 5B-1 group. So, the conclusion are as follows: strains T1-5, T1-9 and 5B-1 can promote intestinal peristalsis, regulate the structure of intestinal microbiota, and alleviate constipation in mice.
- Lactobacillus paracasei,
- constipation,
- laxative function,
- intestinal microbiota,
- 16S rDNA

FullText(HTML)

References (33)

References

[1]	LACY B E, MEARIN F, CHANG L, et al. Bowel disorders[J]. Gastroenterology,2016,150(6):1393−1407. doi: 10.1053/j.gastro.2016.02.031
[2]	WERTH B. Epidemiology of constipation in adults:Why estimates of prevalence differ[J]. Journal of Epidemiological Research,2019,5(1):37−49. doi: 10.5430/jer.v5n1p37
[3]	张升彦, 张磊, 朱良如, 等. 基于肠道菌群的慢性便秘治疗进展[J]. 临床消化病杂志,2022,34(2):149−152. [ZHANG Shengyan, ZHANG Lei, ZHU Liangru, et al. Progress in the treatment of chronic constipation based on gut microbiota[J]. Chinese Journal of Clinical Gastroenterology,2022,34(2):149−152.] ZHANG Shengyan, ZHANG Lei, ZHU Liangru, et al. Progress in the treatment of chronic constipation based on gut microbiota[J]. Chinese Journal of Clinical Gastroenterology, 2022, 34（2）: 149−152.
[4]	于淑俊, 张连中, 刘振权. 复合益生菌粉对功能性便秘小鼠润肠通便的作用[J]. 中国微生态学杂志,2023,35(2):142−151. [YU Shujun, ZHANG Lianzhong, LIU Zhenquan. The effect of compound probiotic powder on moistening the intestines and relieving constipation in mice with functional constipation[J]. Chinese Journal of Microbiology,2023,35(2):142−151.] YU Shujun, ZHANG Lianzhong, LIU Zhenquan. The effect of compound probiotic powder on moistening the intestines and relieving constipation in mice with functional constipation[J]. Chinese Journal of Microbiology, 2023, 35（2）: 142−151.
[5]	张俊, 赵保堂, 杨富民. 副干酪乳杆菌的益生特性及其应用研究进展[J]. 包装与食品机械,2019,37(4):47−52. [ZHANG Jun, ZHAO Baotang, YANG Fumin. Research progress on the probiotic characteristics and application of Lactobacillus paracasei[J]. Packaging and Food Machinery,2019,37(4):47−52.] ZHANG Jun, ZHAO Baotang, YANG Fumin. Research progress on the probiotic characteristics and application of Lactobacillus paracasei[J]. Packaging and Food Machinery, 2019, 37（4）: 47−52.
[6]	和明涓, 鲍悦悦, 倪敏. 干酪乳杆菌治疗功能性便秘的研究进展[J]. 临床医学研究与实践,2023,8(23):191−194. [HE Mingjuan, BAO Yueyue, NI Min, et al. Research progress on the treatment of functional constipation with Lactobacillus casei[J]. Clinical Medical Research and Practice,2023,8(23):191−194.] HE Mingjuan, BAO Yueyue, NI Min, et al. Research progress on the treatment of functional constipation with Lactobacillus casei[J]. Clinical Medical Research and Practice, 2023, 8（23）: 191−194.
[7]	LU Y, ZHANG J, ZHANG Z, et al. Konjac glucomannan with probiotics acts as a combination laxative to relieve constipation in mice by increasing short-chain fatty acid metabolism and 5-hydroxytryptamine hormone release[J]. Nutrition,2021,84(1):1−13.
[8]	陈建国, 李周勇, 李桂花, 等. 热灭活副干酪乳杆菌Lc19对小鼠便秘的缓解作用研究[J]. 中国奶牛,2019(9):12−15. [CHEN Jianguo, LI Zhouyong, LI Guihua, et al. Study on the relieving effect of heat-killed Lactobacillus paracasei Lc19 on constipated mice[J]. China Dairy Cattle,2019(9):12−15.] CHEN Jianguo, LI Zhouyong, LI Guihua, et al. Study on the relieving effect of heat-killed Lactobacillus paracasei Lc19 on constipated mice[J]. China Dairy Cattle, 2019（9）: 12−15.
[9]	曹永强, 张健, 郑喆, 等. 副干酪乳杆菌N1115发酵乳对小鼠便秘的改善作用[J]. 食品科学,2018,39(1):185−191. [CAO Yongqiang, ZHANG Jian, ZHENG Zhe, et al. Improvement effect of fermented milk from Lactobacillus paracasei N1115 on constipation in mice[J]. Food Science,2018,39(1):185−191.] CAO Yongqiang, ZHANG Jian, ZHENG Zhe, et al. Improvement effect of fermented milk from Lactobacillus paracasei N1115 on constipation in mice[J]. Food Science, 2018, 39（1）: 185−191.
[10]	王晓成, 张明, 陈善斌, 等. 副干酪乳杆菌L9对小鼠肠道短链脂肪酸含量的影响[J]. 食品科学,2017,38(13):238−243. [WANG Xiaocheng, ZHANG Ming, CHEN Shanbin, et al. The effect of Lactobacillus paracasei L9 on the content of intestinal short chain fatty acids in healthy mice[J]. Food Science,2017,38(13):238−243.] WANG Xiaocheng, ZHANG Ming, CHEN Shanbin, et al. The effect of Lactobacillus paracasei L9 on the content of intestinal short chain fatty acids in healthy mice[J]. Food Science, 2017, 38（13）: 238−243.
[11]	周晓丹, 刘爱萍, 张明, 等. 副干酪乳杆菌LC-01对便秘小鼠的通便作用[J]. 乳业科学与技术,2012,35(5):7−11. [ZHOU Xiaodan, LIU Aiping, ZHANG Ming, et al. The laxative effect of Lactobacillus paracasei LC-01 in constipated mice[J]. Journal of Dairy Science and Technology,2012,35(5):7−11.] ZHOU Xiaodan, LIU Aiping, ZHANG Ming, et al. The laxative effect of Lactobacillus paracasei LC-01 in constipated mice[J]. Journal of Dairy Science and Technology, 2012, 35（5）: 7−11.
[12]	BOSNEA L A, KOPSAHELIS N, KOKKALI V, et al. Production of a novel probiotic yogurt by incorporation of L. casei enriched fresh apple pieces, dried raisins and wheat grains[J]. Food and Bioproducts Processing,2017,102:62−71. doi: 10.1016/j.fbp.2016.11.010
[13]	田丰伟, 杨震南, 丁历伟, 等. 副干酪乳杆菌LC01对人体肠道菌群的调节作用[J]. 中国食品学报,2018,18(10):24−30. [TIAN Fengwei, YANG Zhennan, DING Liwei, et al. The regulatory effect of Lactobacillus paracasei LC01 on human intestinal microbiota[J]. Chinese Journal of Food Science,2018,18(10):24−30.] TIAN Fengwei, YANG Zhennan, DING Liwei, et al. The regulatory effect of Lactobacillus paracasei LC01 on human intestinal microbiota[J]. Chinese Journal of Food Science, 2018, 18（10）: 24−30.
[14]	陆文伟, 胡文兵, 曹文金, 等. 干酪乳杆菌LC01对小鼠肠道菌群和肠道转运调节作用的剂量效应[J]. 食品科学,2019,40(15):211−217. [LU Wenwei, HU Wenbing, CAO Wenjin, et al. Dose effect of Lactobacillus casei LC01 on intestinal microbiota and intestinal transport regulation in mice[J]. Food Science,2019,40(15):211−217.] LU Wenwei, HU Wenbing, CAO Wenjin, et al. Dose effect of Lactobacillus casei LC01 on intestinal microbiota and intestinal transport regulation in mice[J]. Food Science, 2019, 40（15）: 211−217.
[15]	LIU T H, TSAI T Y, PAN T M. The anti-periodontitis effects of ethanol extract prepared using Lactobacillus paracasei subsp. paracasei NTU 101[J]. Nutrients,2018,10(4):472. doi: 10.3390/nu10040472
[16]	HUANG S Y, CHEN L H, WANG M F, et al. Lactobacillus paracasei PS23 delays progression of age-related cognitive decline in senescence accelerated mouse prone 8 (SAMP8) mice[J]. Nutrients,2018,10(7):894. doi: 10.3390/nu10070894
[17]	胡艳杰, 李卡, 杨家印, 等. 肝移植患者术后益生菌强化的早期肠内营养使用的最佳证据总结[J]. 中国普外基础与临床杂志,2020,27(1):63−68. [HU Yanjie, LI Ka, YANG Jiayin, et al. Summary of best evidence for early enteral nutrition use enhanced with probiotics in liver transplant patients after surgery[J]. Chinese Journal of Bases and Clinics in General Surgery,2020,27(1):63−68.] HU Yanjie, LI Ka, YANG Jiayin, et al. Summary of best evidence for early enteral nutrition use enhanced with probiotics in liver transplant patients after surgery[J]. Chinese Journal of Bases and Clinics in General Surgery, 2020, 27（1）: 63−68.
[18]	朴善英, 徐小达. 肠道益生菌辅治对非酒精性脂肪性肝硬化患者糖脂代谢、氧化损伤和肝功能的影响[J]. 中国医学工程,2019,27(10):102−104. [PIAO Shanying, XU Xiaoda. The effect of gut probiotic adjuvant therapy on glucose and lipid metabolism, oxidative damage, and liver function in patients with non-alcoholic fatty liver cirrhosis[J]. China Medical Engineering,2019,27(10):102−104.] PIAO Shanying, XU Xiaoda. The effect of gut probiotic adjuvant therapy on glucose and lipid metabolism, oxidative damage, and liver function in patients with non-alcoholic fatty liver cirrhosis[J]. China Medical Engineering, 2019, 27（10）: 102−104.
[19]	李萍, 张丽, 黄柳琴. 糜烂性胃炎幽门螺杆菌阳性患者益生菌联合四联疗法治疗后胃肠道微生态变化观察[J]. 现代消化及介入诊疗,2019,24(9):961−964,968. [LI Ping, ZHANG Li, HUANG Liuqin. Observation of changes in gastrointestinal microbiota after probiotic combined with quadruple therapy in Helicobacter pylori positive patients with erosive gastritis[J]. Modern Digestion & Intervention,2019,24(9):961−964,968.] LI Ping, ZHANG Li, HUANG Liuqin. Observation of changes in gastrointestinal microbiota after probiotic combined with quadruple therapy in Helicobacter pylori positive patients with erosive gastritis[J]. Modern Digestion & Intervention, 2019, 24（9）: 961−964,968.
[20]	NES I F, JOHNSBORG O. Exploration of antimicrobial potential in LAB by genomics[J]. Current Opinion in Biotechnology,2004,15(2):100−104. doi: 10.1016/j.copbio.2004.02.001
[21]	应再军. 术前补充益生菌对肠道手术患者术后肠道菌群及肠黏膜屏障功能的影响[J]. 中国微生态学杂志,2019,31(9):1052−1055. [[YING Zaijun. The effect of preoperative supplementation of probiotics on postoperative gut microbiota and intestinal mucosal barrier function in patients undergoing intestinal surgery[J]. Chinese Journal of Microbiology,2019,31(9):1052−1055.] [YING Zaijun. The effect of preoperative supplementation of probiotics on postoperative gut microbiota and intestinal mucosal barrier function in patients undergoing intestinal surgery[J]. Chinese Journal of Microbiology, 2019, 31（9）: 1052−1055.
[22]	王晓光, 石振东, 王国江. 益生菌联合益生元治疗老年慢性功能性便秘临床分析[J]. 中国临床新医学,2014,7(12):1150−1152. [WANG Xiaoguang, SHI Zhendong, WANG Guojiang. Clinical analysis of probiotics combined with prebiotics in the treatment of elderly chronic functional constipation[J]. Chinese Journal of New Clinical Medicine,2014,7(12):1150−1152.] WANG Xiaoguang, SHI Zhendong, WANG Guojiang. Clinical analysis of probiotics combined with prebiotics in the treatment of elderly chronic functional constipation[J]. Chinese Journal of New Clinical Medicine, 2014, 7（12）: 1150−1152.
[23]	SÁNCHEZ E, NIETO J C, VIDAL S, et al. P0088:Fermented milk containing Lactobacillus paracasei subsp. paracasei CNCM I-1518 reduces bacterial translocation in rats treated with carbon tetrachloride[J]. Scientific Reports,2017,7:45712. doi: 10.1038/srep45712
[24]	CHIANG S S, LIAO J W, PAN T M. Effect of bioactive compounds in Lactobacilli-fermented soy skim milk on femoral bone microstructure of aging mice[J]. J Sci Food Agric,2012,92(2):328−335. doi: 10.1002/jsfa.4579
[25]	YILMAZ I, DOLAR M E, OZPINAR H. Effect of administering kefir on the changes in fecal microbiota and symptoms of inflammatory bowel disease:A randomized controlled trial[J]. The Turkish Journal of Gastroenterology,2019,30(3):242−253.
[26]	ESLAMI M, YOUSEFI B, KOKHAEI P, et al. Are probiotics useful for therapy of Helicobacter pylori diseases?[J]. Comparative Immunology, Microbiology and Infectious Diseases,2019,64:1−30. doi: 10.1016/j.cimid.2019.01.022
[27]	LIU C J, TANG X D, YU J, et al. Gut microbiota alterations from different Lactobacillus probiotic-fermented yoghurt treatments in slow-transit constipation[J]. Journal of Functional Foods,2017,38:110−118. doi: 10.1016/j.jff.2017.08.037
[28]	GOMI A, YAMAJI K, WATANABE O, et al. Bifidobacterium bifidum YIT 10347 fermented milk exerts beneficial effects on gastrointestinal discomfort and symptoms in healthy adults:A double-blind, randomized, placebo-controlled study[J]. Journal of Dairy Science,2018,101(6):4830−4841. doi: 10.3168/jds.2017-13803
[29]	BARROS C P, GUIMARES J T, ESMERINO E A, et al. Paraprobiotics and postbiotics:Concepts and potential applications in dairy products[J]. Current Opinion in Food Science,2020,32:1−8. doi: 10.1016/j.cofs.2019.12.003
[30]	王帅静, 李啸, 刘玲彦, 等. 西藏牦牛粪和乳源中益生菌的筛选与鉴定[J]. 中国酿造,2021,40(7):43−48. [WANG Shuaijing, LI Xiao, LIU Lingyan, et al. Screening and identification of probiotics in Tibetan yak manure and milk source[J]. Chinese Brewing,2021,40(7):43−48.] WANG Shuaijing, LI Xiao, LIU Lingyan, et al. Screening and identification of probiotics in Tibetan yak manure and milk source[J]. Chinese Brewing, 2021, 40（7）: 43−48.
[31]	韩明明, 詹炜, 黄福勇, 等. 砂滤模式下马口鱼消化道细菌的组成、分类与相关性[J]. 浙江农业学报,2023,35(10):2286−2298. [HAN Mingming, ZHAN Wei, HUANG Fuyong, et al. Composition, classification, and correlation of bacteria in the digestive tract of zebrafish under sand filtration mode[J]. Acta Agriculturae Zhejiangensis,2023,35(10):2286−2298.] HAN Mingming, ZHAN Wei, HUANG Fuyong, et al. Composition, classification, and correlation of bacteria in the digestive tract of zebrafish under sand filtration mode[J]. Acta Agriculturae Zhejiangensis, 2023, 35（10）: 2286−2298.
[32]	COX L M, YAMANISHI S, SOHN J, et al. Altering the intestinal microbiota during a critical developmental window has lasting metabolic consequences[J]. Cell,2014,158(4):705−721. doi: 10.1016/j.cell.2014.05.052
[33]	CUEVAS-SIERRA A, RIEZU-BOJ J I, GURUCEAGA E, et al. Sex-specific associations between gut Prevotellaceae and host genetics on adiposity[J]. Microorganisms,2020,8(6):938. doi: 10.3390/microorganisms8060938

Supplements (1)

Supplements
Other Related Supplements

Cited By

Cited by

Periodical cited type(2)

1.	赵利维，王刚，李玉玲，刘昆昂. 黑木耳Y9液体发酵产物冻干粉成分及生物活性分析. 中国食用菌. 2024(03): 72-77 .
2.	刘聪，王吉力特，孙义，苏日古格，马捷，张凡，郭淑文. 蜜瓜籽不同溶剂提取物活性成分及抗氧化能力分析. 中国瓜菜. 2024(11): 101-106 .

Other cited types(0)

Get Citation

PDF

XML

Article Metrics

Article views (93) PDF downloads (34) Cited by(2)

Science and Technology of Food Industry

Effect to Intestinal Microbiota Regulation and Laxative Function to Lactobacillus paracasei

Abstract

References

Supplements

Other Related Supplements

Cited by

Periodical cited type(2)

Other cited types(0)

Catalog

Article Metrics

Export File

Citation

Format

Content