Determination of 47 Pesticide Residues in Tea by QuEChERS Method Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

LIU Huawen; DENG Meiqing; CHEN Beiyan

doi:10.13386/j.issn1002-0306.2023050213

Volume 45 Issue 9

Apr. 2024

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Science and Technology of Food Industry > 2024 > 45(9): 255-264. > DOI: 10.13386/j.issn1002-0306.2023050213

LIU Huawen, DENG Meiqing, CHEN Beiyan. Determination of 47 Pesticide Residues in Tea by QuEChERS Method Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2024, 45(9): 255−264. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050213.

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Determination of 47 Pesticide Residues in Tea by QuEChERS Method Combined with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
Guangxi-Asean Food Inspection Center, Nanning 530029, China
2.
School of Public Health, Guangxi Medical University, Nanning 530022, China
3.
Nanning Health School, Nanning 530409, China

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Received Date: May 17, 2023
Available Online: March 02, 2024

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Abstract

Abstract

An analysis method for simultaneous determination of 47 kinds of pesticide residues in tea was established by QuEChERS method combined with ultra performance liquid chromatography-tandem mass spectrometry. The samples were extracted by acetonitrile containing 1% acetic acid, and purified by QuEChERS method. The 47 pesticides were determined by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with an electrospray ion source (ESI±) in positive and negative ion switching multiple reaction monitoring (MRM), and quantified by matrix-matched external standard method. Method validation revealed satisfactory linearities with correlation coefficients (r) between 0.9971 and 0.9996, and limits of detection were 0.001~0.006 mg/kg while the limits of quantitation were 0.002~0.020 mg/kg, the average recoveries at three spiked levels were in the range of 73.4%~114.3%, with relative standard deviations (n=6) of 0.3%~19.9%. The method is sensitive, accurate, stable, and can meet the requirements for determination of 47 pesticide residues in tea.
- QuEChERS,
- tea,
- pesticide residues,
- ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

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References

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