Mechanism of Cistanches Herba in Treatment of Parkinson’s Disease Based on Network Pharmacology and Molecular Docking

XU Zhaohui; ZOU Yuan; CHEN Ying; LIANG Biao; ZHAO Qing

doi:10.13386/j.issn1002-0306.2021100009

Volume 43 Issue 9

Apr. 2022

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Science and Technology of Food Industry > 2022 > 43(9): 13-22. > DOI: 10.13386/j.issn1002-0306.2021100009

XU Zhaohui, ZOU Yuan, CHEN Ying, et al. Mechanism of Cistanches Herba in Treatment of Parkinson’s Disease Based on Network Pharmacology and Molecular Docking[J]. Science and Technology of Food Industry, 2022, 43(9): 13−22. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021100009.

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Mechanism of Cistanches Herba in Treatment of Parkinson’s Disease Based on Network Pharmacology and Molecular Docking

1.
Shanghai University of Tranditional Chinese Medicine, Shanghai 201203, China
2.
Shanghai YangZhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), Tongji University School of Medicine, Shanghai 201619, China

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Received Date: October 10, 2021
Available Online: March 01, 2022

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Abstract

Abstract

The study aimed to explore the mechanism of Cistanches Herba in treatment of Parkinson’s disease based on network pharmacology and molecular docking. The active components in Cistanches Herba were selected as candidate compounds by the TCMSP. And the target information of Cistanches Herba was obtained by PubChem and sea database. The target information of Parkinson’s disease was obtained by GeneCard database and the common target gene information was obtained by comparing traditional Chinese medicine and disease targets. PPI network was constructed by means of STRING. The key target genes were enriched by GO and KEGG pathway in DAVID 6.8 database. The Cytoscape software was applied to construct the network diagram of traditional Chinese medicine-chemical compound-target. The gene targets in the network were sorted according to the degree value, the first five key targets were selected. Finally, the five key targets were in connection with the effective active components of Cistanches Herba by using AutoDock Vina tool. In the end, the results showed that 6 active components were screened from Cistanches Herba beta-sitosterol, arachidonate, suchilactone, Yangambin, quercetin, Marckine. 308 prediction targets were obtained by intersection, which were analyzed by PPI. 38 key target genes were filtered out, and the key target genes were enriched by GO and KEGG pathway. Finally, it was found that GO rich BP includes intracellular signal transduction; CC analysis mainly included protein complex, mitochondrion; MF results mainly included ATP binding, protein complex binding. KEGG pathway enrichment mainly included PI3K/Akt, miRNAs, HIF-1, TNF, FOXO signal pathways, etc. The first five key targets were MAPK8, ESR1, SRC, JAK2 and AKT1. The results of molecular docking showed that the six active components of Cistanches Herba had good binding activity with five key targets. This study found that Cistanches Herba could play a multi-component, multi-channel and multi-target synergistic role in the treatment of Parkinson's disease by protecting mitochondrial function, regulating protein activity, regulating autophagy and inhibiting neuroinflammatory response.
- Cistanches Herba,
- Parkinson's disease,
- network pharmacology,
- molecular docking

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[1]	中华医学会神经病学分会帕金森病及运动障碍学组, 中国医师协会神经内科医师分会帕金森病及运动障碍学组. 中国帕金森病治疗指南(第四版)[J]. 中华神经科杂志,2020,53(12):973−986. [Chinese Society of Parkinson's disease and Movement Disorders, Parkinson’s Disease and Movement Disorder Section of Neurologist Branch of Chinese Medical Doctor Association. Chinese guidelines for the treatment of Parkinson's disease (fourth edition)[J]. Chin J Neurol,2020,53(12):973−986. doi: 10.3760/cma.j.cn113694-20200331-00233
[2]	LI G, MA J, CUI S, et al. Parkinson's disease in China: A forty-year growing track of bedside work[J]. Translational Neurodegeneration,2019,8(1):22. doi: 10.1186/s40035-019-0162-z
[3]	DENG M, ZHAO J Y, TU P F, et al. Echinacoside rescues the SHSY5Y neuronal cells from TNFα-induced apoptosis[J]. European Journal of Pharmacology,2004,505(1-3):11−18. doi: 10.1016/j.ejphar.2004.09.059
[4]	毕萃萃, 刘银路, 魏芬芬, 等. 肉苁蓉的主要化学成分及生物活性研究进展[J]. 药物评价研究,2019,42(9):1896−1900. [BI C C, LIU Y L, WEI F F, et al. Research progress on main chemical constituents and biological activities of Cistanche deserticola[J]. Drug Evaluation Research,2019,42(9):1896−1900.
[5]	陆艳, 张亚杰, 阮杰, 等. 肉苁蓉颗粒剂对帕金森病大鼠模型黑质纹状体多巴胺能神经元的保护作用研究[J]. 中华中医药学刊,2016,34(12):2927−2931. [LU Y, ZHANG Y J, RUAN J, et al. Nigrostriatal dopaminergic protection of Cistanche Granule on rat model of Parkinson's disease[J]. Chinese Archives of Traditional Chinese Medicine,2016,34(12):2927−2931.
[6]	尹若熙, 李刚, 俞腾飞, 等. 肉苁蓉多糖对东莨菪碱所致学习记忆障碍模型小鼠在突触可塑性方面的保护作用[J]. 中国药理学通报,2014(6):801−806,807. [YIN R X, LI G, YU T F, et al. Effect of polysaccharide from Cistanche deserticola on learning and memory deficits induced by scopolamine under improving synaptic plasticity in mice[J]. Chinese Pharmacological Bulletin,2014(6):801−806,807.
[7]	周志锦. 肉苁蓉的研究进展[J]. 中医药信息,1995,12(5):28−30. [ZHOU Z J. Research progress of Cistanche deserticola[J]. Information of Traditional Chinese Medicine,1995,12(5):28−30.
[8]	LIU H, WANG J, ZHOU W, et al. Systems approaches and polypharmacology for drug discovery from herbal medicines: An example using licorice[J]. Journal of Ethnopharmacology,2013,146(3):773−793. doi: 10.1016/j.jep.2013.02.004
[9]	GRAY M T, WOULFE J M. Striatal blood-brain barrier permeability in Parkinson’s disease[J]. J Cereb Blood Flow Metab,2015,35(5):747−750. doi: 10.1038/jcbfm.2015.32
[10]	陈文娜, 朱爱松, 丛培玮, 等. 基于网络药理学的二陈汤治疗冠心病分子机制研究[J]. 中草药,2019(2):441−448. [CHEN W N, ZHU A S, CONG P W, et al. Molecular mechanism of Erchen decoction in treatment of coronary heart disease based on network pharmacology[J]. Chinese Traditional and Herbal Drugs,2019(2):441−448. doi: 10.7501/j.issn.0253-2670.2019.02.025
[11]	TROTT O, OLSON A J. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading[J]. Journal of Computational Chemistry,2010,31(2):455−461.
[12]	龚普阳, 郭瑜婕, 李晓朋, 等. 基于网络药理学与分子对接技术的金花清感颗粒防治新型冠状病毒肺炎的潜在药效物质研究[J]. 中草药,2020,51(7):1685−1693. [GONG P Y, GUO Y J, LI X P, et al. Exploring active compounds of Jinhua Qinggan Granules for prevention of COVID-19 based on network pharmacology and molecular docking[J]. Chinese Traditional and Herbal Drugs,2020,51(7):1685−1693. doi: 10.7501/j.issn.0253-2670.2020.07.002
[13]	郑晓珂, 刘媛媛, 冯卫生, 等. 天然苯乙醇苷类化合物研究进展[J]. 中国新药杂志,2011,20(3):230−234. [ZHENG X K, LIU Y Y, FENG W S, et al. Development in research of natural phenylethanoid glycosides[J]. Chinese Journal of New Drugs,2011,20(3):230−234.
[14]	赵卿, 张鹏, 白宇, 等. 松果菊苷通过调控GFRα1/AKT信号通路抑制MPP⁺诱导的多巴胺能细胞SH-SY5Y凋亡[J]. 中成药,2016(6):1225−1231. [ZHAO Q, ZHANG P, BAI Y, et al. Echinacoside inhibits apoptosis of SH-SY5Y cell through GFRα1/AKT pathway[J]. Chinese Traditional Patent Medicine,2016(6):1225−1231.
[15]	ZHAO Q, YANG X Y, CAI D F, et al. Echinacoside protects against MPP(+)-induced neuronal apoptosis via ROS/ATF3/CHOP pathway regulation[J]. Neuroscience Bulletin,2016,32(4):349−362. doi: 10.1007/s12264-016-0047-4
[16]	ZHAO Q, GAO J, LI W, et al. Neurotrophic and neurorescue effects of echinacoside in the subacute MPTP mouse model of Parkinson's disease[J]. Brain research,2010,1346:224−236. doi: 10.1016/j.brainres.2010.05.018
[17]	CHENG Y F, CHEN Y P, LI J, et al. Dietary β-sitosterol improves growth performance, meat quality, antioxidant status, and mitochondrial biogenesis of breast muscle in broilers[J]. Animals an Open Access Journal from Mdpi,2019,9(3):71.
[18]	SUBRAMANIAM S R, CHESSELET M F. Mitochondrial dysfunction and oxidative stress in Parkinson's disease[J]. Progress in Neurobiology,2013,106-107(3):17−32.
[19]	WANG Z J, LIANG C L, LI G M, et al. Neuroprotective effects of arachidonic acid against oxidative stress on rat hippocampal slices[J]. Chemico-Biological Interactions,2006,163(3):207−217. doi: 10.1016/j.cbi.2006.08.005
[20]	王德杰, 郑傲. 槲皮素对D-半乳糖致衰老小鼠模型学习记忆及脑内炎症的影响[J]. 中华老年多器官疾病杂志,2018,17(9):691−695. [WANG D J, ZHENG A. Effects of quercetin on learning and memory and cerebral inflammation in the D-galactose-induced aging mice[J]. Chin J Mult Organ Dis Elderly,2018,17(9):691−695. doi: 10.11915/j.issn.1671-5403.2018.09.158
[21]	WANG Y H, YU H T, PU X P, et al. Myricitrin alleviates methylglyoxal-induced mitochondrial dysfunction and AGEs/RAGE/NF-κB pathway activation in SH-SY5Y cells[J]. Journal of Molecular Neuroscience:MN,2014,53(4):562−570. doi: 10.1007/s12031-013-0222-2
[22]	BOYINA H K, GEETHAKHRISHNAN S L, PANUGANTI S, et al. In silico and in vivo studies on quercetin as potential anti-Parkinson agent[J]. Advances in Experimental Medicine and Biology,2020,1195:1−11.
[23]	LUO Y, HOFFER A, HOFFER B, et al. Mitochondria: A therapeutic target for Parkinson’s disease?[J]. International Journal of Molecular Sciences,2015,16(9):20704−20730. doi: 10.3390/ijms160920704
[24]	GANGULY G, CHAKRABARTI S, CHATTERJEE U, et al. Proteinopathy, oxidative stress and mitochondrial dysfunction: Cross talk in Alzheimer’s disease and Parkinson's disease[J]. Drug Design, Development and Therapy,2017,2017(11):797−810.
[25]	TIMMONS S, COAKLEY M F, MOLONEY A M, et al. Akt signal transduction dysfunction in Parkinson's disease[J]. Neuroscience Letters,2009,467(1):30−35. doi: 10.1016/j.neulet.2009.09.055
[26]	林瑶, 杨莎莎, 刘婷, 等. 肉苁蓉对帕金森病模型大鼠PI3K、Akt、Bcl-2、Bax蛋白表达的影响[J]. 江西中医药大学学报,2019,31(1):83−86. [LIN Y, YANG S S, LIU T, et al. Effects of HerbaCistanche on the protein expression of PI3K, Akt, Bcl-2 and Bax in rats model of Parkinson's disease[J]. Journal of Jiangxi University of TCM,2019,31(1):83−86.
[27]	YANG P H, ZHU J X, HUANG Y D, et al. Human basic fibroblast growth factor inhibits Tau phosphorylation via the PI3K/Akt-GSK3β signaling pathway in a 6-Hydroxydopamine-induced model of Parkinson's disease[J]. Neurodegenerative Diseases,2016,16(5-6):357−369. doi: 10.1159/000445871
[28]	QIAO C M, SUN M F, JIA X B, et al. Sodium butyrate causes α-synuclein degradation by an Atg5-dependent and PI3K/Akt/mTOR-related autophagy pathway[J]. Experimental Cell Research,2019,387(1):111772.
[29]	PINO E, AMAMOTO R, ZHENG L, et al. FOXO3 determines the accumulation of α-synuclein and controls the fate of dopaminergic neurons in the substantia nigra[J]. Human Molecular Genetics,2014,23(6):1435−1452. doi: 10.1093/hmg/ddt530
[30]	BRIGGS C E, WANG Y, KONG B, et al. Midbrain dopamine neurons in Parkinson's disease exhibit a dysregulated miRNA and target-gene network[J]. Brain Research,2015,1618:111−121. doi: 10.1016/j.brainres.2015.05.021
[31]	DOXAKIS E. Post-transcriptional regulation of alpha-Synuclein expression by mir-7 and mir-153[J]. Journal of Biological Chemistry,2010,285(17):12726−12734. doi: 10.1074/jbc.M109.086827
[32]	HU Y B, ZHANG Y F, WANG H, et al. MiR-425 deficiency promotes necroptosis and dopaminergic neurodegeneration in Parkinson's disease[J]. Cell Death & Disease,2019,10(8):589.
[33]	SCHUMACKER P T. Hypoxia-inducible factor-1 (HIF-1)[J]. Critical Care Medicine,2005,33(5):423−425.
[34]	WU Y C, LI X Q, XIE W J, et al. Neuroprotection of deferoxamine on rotenone-induced injury via accumulation of HIF-1α and induction of autophagy in SH-SY5Y cells[J]. Neurochemistry International,2010,57(3):198−205. doi: 10.1016/j.neuint.2010.05.008
[35]	FENG Y, LIU T, LI X Q, et al. Neuroprotection by orexin-A via HIF-1α induction in a cellular model of Parkinson's disease[J]. Neuroscience Letters,2014,579:35−40. doi: 10.1016/j.neulet.2014.07.014
[36]	ALAM Q, ALAM M Z, MUSHTAQ G, et al. Inflammatory process in Alzheimer’s and Parkinson’s diseases: Central role of cytokines[J]. Current Pharmaceutical Design,2016,22(5):541−548. doi: 10.2174/1381612822666151125000300
[37]	YANG L, MAO K, YU H, et al. Neuroinflammatory responses and Parkinson’ disease: Pathogenic mechanisms and therapeutic targets[J]. Journal of Neuroimmune Pharmacology,2020,15(4):830−837. doi: 10.1007/s11481-020-09926-7
[38]	LIANG Y, CHEN C, XIA B, et al. Neuroprotective effect of echinacoside in subacute mouse model of Parkinson's disease[J]. BioMed Research International,2019,2019:1−8.
[39]	LIU J, LIU W, LU Y, et al. Piperlongumine restores the balance of autophagy and apoptosis by increasing BCL2 phosphorylation in rotenone-induced Parkinson disease models[J]. Autophagy,2018,14(5):845−861. doi: 10.1080/15548627.2017.1390636
[40]	CHAKRABARTI M, HAQUE A, BANIK N L, et al. Estrogen receptor agonists for attenuation of neuroinflammation and neurodegeneration[J]. Brain Research Bulletin,2014,109:22−31. doi: 10.1016/j.brainresbull.2014.09.004
[41]	LABANDEIRA-GARCIA J L, RODRIGUEZ-PEREZ A I, VALENZUELA R, et al. Menopause and Parkinson's disease. Interaction between estrogens and brain renin-angiotensin system in dopaminergic degeneration[J]. Frontiers in Neuroendocrinology,2016,43:44−59. doi: 10.1016/j.yfrne.2016.09.003
[42]	YANG H, WANG L, ZANG C, et al. Src inhibition attenuates neuroinflammation and protects dopaminergic neurons in Parkinson’s disease models[J]. Frontiers in Neuroscience,2020,14:45. doi: 10.3389/fnins.2020.00045
[43]	RAN C, WESTERLUND M, ANVRET A, et al. Genetic studies of the protein kinase AKT1 in Parkinson's disease[J]. Neuroscience Letters,2011,501(1):41−44. doi: 10.1016/j.neulet.2011.06.038
[44]	AHMAD F, NIDADAVOLU P, DURGADOSS L, et al. Critical cysteines in Akt1 regulate its activity and proteasomal degradation: Implications for neurodegenerative diseases[J]. Free Radical Biology & Medicine,2014,74:118−128.

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Mechanism of Cistanches Herba in Treatment of Parkinson’s Disease Based on Network Pharmacology and Molecular Docking