Protective Effects of Fucoidan Isolated from <i>Sargassum fusiform</i> on AAPH-induced Antioxidant Response in Zebrafish Model

WANG Shengnan; FU Xiaoting; XU Jiachao; GAO Xin

doi:10.13386/j.issn1002-0306.2020120007

Volume 42 Issue 18

Sep. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(18): 356-365. > DOI: 10.13386/j.issn1002-0306.2020120007

WANG Shengnan, FU Xiaoting, XU Jiachao, et al. Protective Effects of Fucoidan Isolated from Sargassum fusiform on AAPH-induced Antioxidant Response in Zebrafish Model[J]. Science and Technology of Food Industry, 2021, 42(18): 356−365. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120007.

Citation:

PDF (5020 KB)

Protective Effects of Fucoidan Isolated from Sargassum fusiform on AAPH-induced Antioxidant Response in Zebrafish Model

College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

More Information

Received Date: December 01, 2020
Available Online: July 19, 2021

Graphical Abstract

Abstract

Abstract

Objective: A 2,2-azobis(2-methylpropylimidazole) dihydrochloride(AAPH)-stimulated zebrafish model was studied and used for investigating the antioxidant activities of a fucoidan(SFPS) isolated from Sargassum fusiform(S. fusiform). Methods: SFPS extracted from S. fusiform and the chemical composition of SFPS were determined. The scavenging capacities of SFPS on 1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt(ABTS) radicals were determined to evaluate its antioxidant activity in vitro. Then the AAPH-induced oxidative stress model of zebrafish was studied and optimized. Three phenotypic indexes(survival rate, yolk sac size and heartbeat rate) as well as levels of cell death and reactive oxygen species(ROS) were used to evaluate the antioxidant activity of SFPS in vivo. Results: The SFPS contained 75.30%±1.77% carbohydrates content, 21.39%±1.07% sulfate content, 1.78%±0.19% protein content and 1.47%±0.02% phenolic content. Compared with the crude polysaccharide(SFPSC), lower IC₅₀ values of SFPS for DPPH and ABTS were determined to be 0.59 and 0.69 mg/mL, respectively. Besides, SFPS was nontoxic to zebrafish embryos in the range of 50~200 μg/mL and dose-dependently protect zebrafishfrom AAPH induced oxidative damage. At the optimal concentration of 200 μg/mL, the survival rate of zebrafish embryos was significantly increased(100%, P<0.05), the heartbeat rate and yolk sac size were significantly reduced(101.37% and 111.80%, respectively, P<0.05), and the inhibition rate of AAPH-induced cell death and ROS production in zebrafish were up to 70.55% and 50.68%, respectively. Conclusion: SFPS would have strong antioxidant activity in vitro, and had superior antioxidant ability and oxidative damage repair ability in vivo, thus indicated that SFPS as a natural antioxidant would have a wide application prospect in the field of health food and cosmetics.
- fucoidan,
- Sargassum fusiform,
- zebrafish,
- oxidative stress,
- 2,2-azobis(2-methylpropylimidazole) dihydrochloride

FullText(HTML)

References (42)

References

[1]	倪立颖, 邹娅雪, 付晓婷, 等. 利用LPS诱导胚胎期斑马鱼炎症模型研究羊栖菜多酚抗炎机制[J]. 食品工业科技,2019,40(21):279−285. [Ni Liying, Zou Yaxue, Fu Xiaoting, et al. Anti-inflammatory mechanism of phenolic compounds from Sargassum fusiforme by LPS-induced zebrafish embryo model[J]. Science and Technology of Food Industry,2019,40(21):279−285.
[2]	Zhang R, Zhang X, Tang Y, et al. Composition, isolation, purification and biological activities of Sargassum fusiforme polysaccharides: A review[J]. Carbohydrate Polymers,2020,228:115381. doi: 10.1016/j.carbpol.2019.115381
[3]	Draget K I, Taylor C. Chemical, physical and biological properties of alginates and their biomedical implications[J]. Food Hydrocolloids,2011,25(2):251−256.
[4]	Yu W, Maochen X, Qi C, et al. Biological activities of fucoidan and the factors mediating its therapeutic effects: A review of recent studies[J]. Marine Drugs,2019,3(17):183.
[5]	Chen L, Chen P, Jian L, et al. Sargassum fusiforme polysaccharide SFP-F2 activates the NF-κB signaling pathway via CD14/IKK and P38 Axes in RAW264.7 Cells[J]. Marine Drugs,2018,16(8):264.
[6]	Wang L, Oh J Y, Jayawardena T U, et al. Anti-inflammatory and anti-melanogenesis activities of sulfated polysaccharides isolated from Hizikia fusiforme: Short communication[J]. International Journal of Biological Macromolecules,2020,142:542−550.
[7]	Cheng Y, Sibusiso L, Hou L, et al. Sargassum fusiforme fucoidan modifies the gut microbiota during alleviation of streptozotocin-induced hyperglycemia in mice[J]. International Journal of Biological Macromolecules,2019:131.
[8]	赵子慧, 徐曼, 刘阿梅, 等. 羊栖菜多糖通过激活JNK/Nrf2/ARE信号通路延缓小鼠衰老进程作用研究[J]. 中草药,2018,49(23):5600−5609. [Zhao Zihui, Xu Man, Liu Amei, et al. Effect of Sargassum fusiforme polysaccharides on activating JNK/Nrf2/ARE signaling pathway and slowing down aging process[J]. Chinese Traditional and Herbal Drugs,2018,49(23):5600−5609. doi: 10.7501/j.issn.0253-2670.2018.23.018
[9]	Li Y, Chen B, Wu W, et al. Antioxidant and antimicrobial evaluation of carboxymethylated and hydroxamated degraded polysaccharides from Sargassum fusiforme[J]. International Journal of Biological Macromolecules,2018,118:1550−1557. doi: 10.1016/j.ijbiomac.2018.06.196
[10]	Wang L, Oh J Y, Yang H W, et al. Protective effect of sulfated polysaccharides from a celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafsh[J]. Marine Life Science & Technology,2019(1):104−111.
[11]	吴娟, 欧志荣, 李昭蓉, 等. 稀酸提取羊栖菜多糖的结构及其抗氧化特性研究[J]. 福建农业学报,2019,34(7):842−851. [Wu Juan, Ou Zhirong, Zhao Mouming. Structure and antioxidant activity of polysaccharides extracted from Sargassum fusiforme[J]. Fujian Journal of Agricultural Sciences,2019,34(7):842−851.
[12]	吴利敏, 夏盛隆, 申苏建,等. L02脂肪变模型中氧化应激的发生及羊栖菜多糖的干预作用[J]. 中国现代医生,2017,34(55):17−23. [Wu Limin, Xia Shenglong, Shen sujian, et al. The occurrence of oxidative stress in L02 fatty model and the intervention of Sargassum fusiform polysaccharide[J]. China Modern Doctor,2017,34(55):17−23.
[13]	Wang W, Lu J, Wang C, et al. Effects of Sargassum fusiforme polysaccharides on antioxidant activities and intestinal functions in mice[J]. International Journal of Biological Macromolecules,2013,58:127−132. doi: 10.1016/j.ijbiomac.2013.03.062
[14]	Wang L, Oh J Y, Kim H S, et al. Protective effect of polysaccharides from celluclast-assisted extract of Hizikiafusiforme against hydrogen peroxide-induced oxidative stress in vitro in Vero cells and in vivo in zebrafish[J]. International Journal of Biological Macromolecules,2018,112:483−489. doi: 10.1016/j.ijbiomac.2018.01.212
[15]	董乐, 董笑瀛, 王芳, 等. 羊栖菜硫酸多糖的超声辅助提取工艺优化及抗氧化活性研究[J]. 食品工业科技,2015,36(12):265−269. [Dong Le, Dong Xiaoying, Wang Fang, et al. Optimization of extraction and antioxidative activity in vitro ofsulfated polysaccharides from Sargassum fusiforme(Hary) Setch[J]. Science and Technology of Food Industry,2015,36(12):265−269.
[16]	Weihua J, Wenjing Z, Jing W, et al. A study of neuroprotective and antioxidant activities of heteropolysaccharides from six Sargassum species[J]. International Journal of Biological Macromolecules,2014,67:336−342. doi: 10.1016/j.ijbiomac.2014.03.031
[17]	Kim E, Kang M, Lee J, et al. Protective effect of marine brown algal polyphenols against oxidative stressed zebrafish with high glucose[J]. RSC Advances,2015,5:25738−25746. doi: 10.1039/C5RA00338E
[18]	Lee S, Ko C, Jee Y, et al. Anti-inflammatory effect of fucoidan extracted from Ecklonia cava in zebrafish model[J]. Carbohydrate Polymers,2013,92(1):84−89. doi: 10.1016/j.carbpol.2012.09.066
[19]	Lieschke G J, Currie P D. Animal models of human disease: Zebrafish swim into view[J]. Nature Reviews Genetics,2007,8(5):353−367. doi: 10.1038/nrg2091
[20]	Schoonheim P J, Chatzopoulou A, Schaaf M J M. The zebrafish as an in vivo model system for glucocorticoid resistance[J]. Steroids,2010,75(12):918−925. doi: 10.1016/j.steroids.2010.05.010
[21]	Eisen J S. Zebrafish make a big splash[J]. Cell,1996,87(6):969−977. doi: 10.1016/S0092-8674(00)81792-4
[22]	Wang L, Oh J Y, Hwang J, et al. In vitro and in vivo antioxidant activities of polysaccharides isolated from celluclast-assisted extract of an edible brown seaweed, Sargassum fulvellum[J]. Antioxidants,2019,8(10):493. doi: 10.3390/antiox8100493
[23]	Kang M, Cha S H, Wijesinghe W A J P, et al. Protective effect of marine algae phlorotannins against AAPH-induced oxidative stress in zebrafish embryo[J]. Food Chemistry,2013,138(2−3):950−955. doi: 10.1016/j.foodchem.2012.11.005
[24]	Ni Liying, Wang Lei, Fu Xiaoting, et al. In vitro and in vivo anti-inflammatory activities of a fucose-rich fucoidan isolated from Saccharina japonica[J]. International Journal of Biological Macromolecules,2020,156:717−729. doi: 10.1016/j.ijbiomac.2020.04.012
[25]	李雅静. 两品系羊栖菜(Sargassum fusiforme)的营养品质及活性成分研究[D]. 青岛: 中国海洋大学, 2018. Li Yajing. Study on nutritional quality and active components of two strands of Sargassum fusiforme[D]. Qingdao: Ocean University of China, 2018.
[26]	Dubois M, Gilles K A, Hamilton J K, et al. Colorimetric method for determination of sugars and related substances[J]. American Chemical Society,1956,3(28):350−356.
[27]	Kawai Y, Seno N, Anno K. A modified method for chondrosulfatase assay[J]. Analytical Biochemistry,1969(32):314−321.
[28]	Winters A L, Minchin F R. Modification of the lowry assay to measure proteins and phenols in covalently bound complexes[J]. Analytical Biochemistry,2005,346(1):43−48. doi: 10.1016/j.ab.2005.07.041
[29]	Chandler S F, Dodds J H. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of Solanum laciniatum[J]. Plant Cell Reports,1983,2(4):205−208. doi: 10.1007/BF00270105
[30]	Tierney M S, Smyth T J, Rai D K, et al. Enrichment of polyphenol contents and antioxidant activities of Irish brown macroalgae using food-friendly techniques based on polarity and molecular size[J]. Food Chemistry,2013,139(1−4):753−761. doi: 10.1016/j.foodchem.2013.01.019
[31]	Li Y, Fu X, Duan D, et al. Extraction and identification of phlorotannins from the brown alga, Sargassum fusiforme(Harvey) setchell[J]. Marine Drugs,2017,15(2):49. doi: 10.3390/md15020049
[32]	Frattaruolo L, Carullo G, Brindisi M, et al. Antioxidant and anti-inflammatory activities of flavanones from Glycyrrhiza glabra L. (licorice) leaf phytocomplexes: Identification of licoflavanone as a modulator of nf-kb/mapk pathway[J]. Antioxidants,2019,8:186. doi: 10.3390/antiox8060186
[33]	邹娅雪, 付晓婷, 段德麟, 等. 利用斑马鱼模型研究琼胶寡糖抗氧化机制[J]. 食品工业科技,2019(4):286−298. [Zou Yaxue, Fu Xiaoting, Duan Deilin, et al. Antioxidant activities of agaro-oligosaccharides in AAPH-induced zebrafish model[J]. Science and Technology of Food Industry,2019(4):286−298.
[34]	Zou Y, Fu X, Liu N, et al. The synergistic anti-inflammatory activities of agaro-oligosaccharides with different degrees of polymerization[J]. Journal of Applied Phycology,2019,31(4):2547−2558. doi: 10.1007/s10811-019-1740-2
[35]	Na Yi-rang, Seok Seung-hyeok, Bae Min-won, et al. Protective effects of vitamin E against 3, 3', 4, 4', 5-pentachlorobiphenyl (PCB126) induced toxicity in zebrafish embryos[J]. Ecotoxicology and Environmental Safety,2009,72(3):714−719. doi: 10.1016/j.ecoenv.2008.09.015
[36]	Tae-Young C, Jin-Hwa K, Han K D, et al. Zebrafish as a new model for phenotype-based screening of melanogenic regulatory compounds[J]. Pigment cell Research,2007,20(2):120−127. doi: 10.1111/j.1600-0749.2007.00365.x
[37]	Jacobsen C, Sørensen, Ann-Dorit M, et al. Source, extraction, characterization, and applications of novel antioxidants from seaweed[J]. Annual Review of Food Science and Technology,2019,10(1).
[38]	Dion M Z, Wang Y J, Bregante D, et al. The use of a 2,2'-azobis(2-amidinopropane) dihydrochloride stress model as an indicator of oxidation susceptibility for monoclonal antibodies[J]. Journal of Pharmaceutical Ences,2018,107(2):550−558.
[39]	Betigeri S, Thakur A, Raghavan K. Use of 2, 2′-azobis(2-amidinopropane) dihydrochloride as a reagent tool for evaluation of oxidative stability of drugs[J]. Pharmaceutical Research,2005,22(2):310−317. doi: 10.1007/s11095-004-1199-x
[40]	陈汝家, 朱俊靖, 周盛梅, 等. 斑马鱼模型在药物毒性与安全性评价中的应用[J]. 毒理学杂志,2012,26(3):224−228. [Chen Rujia, Zhu Junjing, Zhou Shengmei. Application of zebrafish model in drug toxicity and safety evaluation[J]. Journal of Toxical,2012,26(3):224−228.
[41]	Phull A, Majid M, Haq I, et al. In vitro and in vivo evaluation of anti-arthritic, antioxidant efficacy of fucoidan from Undaria pinnatifida (Harvey) Suringar[J]. International Journal of Biological Macromolecules,2017,97:468−480. doi: 10.1016/j.ijbiomac.2017.01.051
[42]	Lee W, Kang N, Kim E, et al. Radioprotective effects of a polysaccharide purified from Lactobacillus plantarum-fermented Ishigeokamurae against oxidative stress caused by gamma ray-irradiation in zebrafish in vivo model[J]. Journal of Functional Foods,2017,28:83−89. doi: 10.1016/j.jff.2016.11.004

[1]	WU Li, ZHANG Yuan, TANG Chunhong, YOU Huan, CHANG Haijun, ZHANG Shuai. Effect of Whole Wheat Noodles on Biochemical Indexes and Histopathology of Type 2 Diabetic Rats[J]. Science and Technology of Food Industry, 2025, 46(3): 386-393. DOI: 10.13386/j.issn1002-0306.2024030144
[2]	ZHOU Xiran, JIAO Gena, ZENG Ji, LI Bohan, SHEN Lu. Effect of Yujing Jiangtang Recipe on Blood Glucose, Lipid and Serum Oxidative Stress Related Indexes in Type 2 diabetes Rats[J]. Science and Technology of Food Industry, 2023, 44(19): 433-439. DOI: 10.13386/j.issn1002-0306.2022120224
[3]	LU Yajun, LIU Ying, WANG Yi, HUANG Wen. Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP[J]. Science and Technology of Food Industry, 2023, 44(12): 397-404. DOI: 10.13386/j.issn1002-0306.2022070173
[4]	NI Jun, YUAN Cailian, SHE Rong, ZHAO Chengfa, LI Lijuan, YANG Xu, YANG Xiaoyan. Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells[J]. Science and Technology of Food Industry, 2023, 44(5): 60-66. DOI: 10.13386/j.issn1002-0306.2022050222
[5]	ZHAO Yuezhu, JIN Xin, ZHANG Yunan, LI Jingshuang, YU Yang. Protective Effect of Aloe Polysaccharide on Oxidative Stress Injury of HepG2 Cells Induced by D-galactose[J]. Science and Technology of Food Industry, 2023, 44(1): 405-412. DOI: 10.13386/j.issn1002-0306.2022040037
[6]	DING Lina, WANG Zhibin, WANG Yao, NING Jie, ZHANG Xiandang, DING Wenyu. Research Progress on the Utilization of Vitamin C Against Type 2 Diabetes[J]. Science and Technology of Food Industry, 2021, 42(15): 372-376. DOI: 10.13386/j.issn1002-0306.2020070147
[7]	LIU Yinlu, YANG Litao, BI Cuicui, WEI Fenfen, ZHANG Bo. Protective Effect of Nostoc sphaeroids Kütz on Oxidative Stress in Hyperlipidemic Mice[J]. Science and Technology of Food Industry, 2021, 42(14): 320-327. DOI: 10.13386/j.issn1002-0306.2020090140
[8]	ZOU Ya-xue, FU Xiao-ting, Duan De-lin, XU Jia-chao, GAO Xin, Wang Xue-liang. Antioxidant Activities of Agaro-oligosaccharides in AAPH-induced Zebrafish Model[J]. Science and Technology of Food Industry, 2019, 40(4): 286-291,298. DOI: 10.13386/j.issn1002-0306.2019.04.048
[9]	MENG Xiao, JIANG Li-shi, CHEN Yan, LIU Shu-kun. Effect of different test substances from Lactobacillus plantarum SCS2 on blood glucose and oxidative stress in mice[J]. Science and Technology of Food Industry, 2018, 39(8): 267-271. DOI: 10.13386/j.issn1002-0306.2018.08.049
[10]	LI Qing, LIU Jian- lei, JING Hao. Comparison of ovotransferrin and lactoferrin in oxidative stability[J]. Science and Technology of Food Industry, 2016, (05): 91-97. DOI: 10.13386/j.issn1002-0306.2016.05.010

Supplements (0)

Cited By

Cited by

Periodical cited type(5)

1.	陈酉，王素娟，张飞宇，杨林林，乔璐，董诚明，董宁. 基于斑马鱼模型的丹参花中酚酮类成分美白及抗氧化效果探究. 时珍国医国药. 2024(08): 1868-1871 .
2.	何维彬，高亮，艾玉廷，周建富，龚行楚，杨振中，蒋剑平. 羊栖菜化学成分及药理活性研究进展. 中草药. 2023(07): 2327-2337 .
3.	冯敏，幸宏伟，尤琳烽，刘小娟. 洛神花花青素提取工艺及抗氧化活性研究. 重庆工商大学学报(自然科学版). 2023(06): 1-7 .
4.	匡婷，方宗穆，张名位，张瑞芬，池建伟，黄菲，贾栩超，董丽红，马勤，邓梅，马永轩，郝娟，赵东. 羊栖菜腥味物质分析及其脱除方法比较. 现代食品科技. 2022(11): 285-297 .
5.	梁美娜，吴明江，陈胜，苏来金. 羊栖菜多糖的提取纯化及功能活性研究进展. 食品安全质量检测学报. 2021(21): 8305-8312 .