Screening of Uric Acid-lowering Food and Medicinal Materials Based on Inhibiting Xanthine Oxidase Activity and Zebrafish Hyperuricemia Model

ZHANG Yingyu; PUBU Duoji; LU Cong; WANG Fengzhong

doi:10.13386/j.issn1002-0306.2020080220

Volume 42 Issue 12

Jun. 2021

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Science and Technology of Food Industry > 2021 > 42(12): 334-339. > DOI: 10.13386/j.issn1002-0306.2020080220

ZHANG Yingyu, PUBU Duoji , LU Cong, et al. Screening of Uric Acid-lowering Food and Medicinal Materials Based on Inhibiting Xanthine Oxidase Activity and Zebrafish Hyperuricemia Model[J]. Science and Technology of Food Industry, 2021, 42(12): 334−339. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080220.

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Screening of Uric Acid-lowering Food and Medicinal Materials Based on Inhibiting Xanthine Oxidase Activity and Zebrafish Hyperuricemia Model

ZHANG Yingyu^{1, 2,},
PUBU Duoji¹,
LU Cong²,
WANG Fengzhong^{1, 2, ,}

1.
Institute of Food Science and Technology, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China
2.
Institute of Food Science and Technology CAAS, Beijing 100193, China

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Received Date: August 23, 2020
Available Online: April 19, 2021

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Abstract

Abstract

Through the combination of in vitro (the xanthine oxidase inhibitory activity) and in vivo (a zebrafish hyperuricemia model) methods, the uric acid-lowering activity of 15 edible herbs crude ethanol extracts was screened. In vitro experiment set up blank group, enzyme reaction group, inhibitor group and control group. The absorbance at 295 nm with microplate reader was measured. In vivo experiment 5 days post fertilization zebrafish larvae with normal development were randomly divided into blank group, model group (200 μmol/L PO+10 μmol/L XSS), edible herbs group (200 μmol/L PO+10 μmol/L XSS+extracts of different concentrations) and positive control group (200 μmol/L PO+10 μmol/L XSS+APL 2 mmol/L). All groups were cultivated at 28.5 ℃ for 24 h to determine the uric acid contents and further verify the uric acid-lowering activity. In vitro experiment showed that 15 ethanol extracts were inhibitory, with 8 having greater than 50% inhibitory rate at 400 μm/mL. The most active one was the ethanol extracts of the Rhodiola crenulate, which was followed closely by Lagotis brevituba, Poeania suffruticosa, Patrinia scabiosaefolia, Phellodendron chinense, Scindapsus aureus, Cassia tora and Coreopsis tinctorial. In vivo experiment showed that the uric acid level of all these eight extract groups were significantly lower than the model group (P<0.05 or P<0.01).
- uric acid-lowering activity,
- xanthine oxidase,
- zebrafish hyperuricemia model,
- activity screening,
- edible herbs

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