CHEN Cheng. Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-Glucosidase[J]. Science and Technology of Food Industry, 2022, 43(7): 248−254. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090146.
Citation: CHEN Cheng. Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-Glucosidase[J]. Science and Technology of Food Industry, 2022, 43(7): 248−254. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090146.

Optimization of Extraction Process of Schisandra chinensis Polysaccharide and Analysis of Its Inhibitory Activity Against α-Glucosidase

More Information
  • Received Date: September 09, 2021
  • Available Online: February 10, 2022
  • The effect of ultrasonic power, extraction temperature, compound enzyme dosage and extraction time on the yield of polysaccharide from Schisandra chinensis were investigated by single factor experiment. The ultrasonic assisted complex enzyme extraction of polysaccharides from Schisandra chinensis was optimized by orthogonal experiment based on the results of single factor experiment. The inhibitory activity of Schisandra chinensis polysaccharide on α-glucosidase was analyzed by enzyme inhibition and fluorescence spectroscopy. The results showed that the optimum extraction parameters to achieve the highest polysaccharide yield (8.12%±0.10%) from Schisandra chinensis by ultrasonic-assisted extraction of complex enzyme were obtained under a ultrasonic power of 200 W, extraction temperature of 30 ℃, enzyme dosage of 0.20% and extraction time of 40 min. The IC50 of Schisandra chinensis polysaccharide on α-glucosidase inhibition was 27.15 μg/mL, and α-glucosidase activity was inhibited by a mixture of competitive and non competitive. Meanwhile, Schisandra chinensis polysaccharide could cause fluorescence quenching of α-glucosidase. The results provide a theoretical basis for the further development of Schisandra chinensis.
  • [1]
    薛俊礼, 吕洋, 杨艳艳, 等. 微波辅助复合酶法提取北五味子多糖的工艺研究[J]. 河南工业大学学报(自然科学版),2020,41(6):61−67. [XUE J L, LV Y, YANG Y Y, et al. Study on extraction technology of Schisandra chinensis Baill. polysaccharide using microwave-assisted complex enzymatic method[J]. Journal of Henan University of Technology(Natural Science Edition),2020,41(6):61−67.
    [2]
    许梦然, 王迦琦, 高婧雯, 等. 北五味子多糖提取工艺优化及其对LPS刺激巨噬细胞线粒体膜电位的保护作用[J]. 食品工业科技,2020,41(20):33−40. [XU M R, WANG J Q, GAO J W, et al. Optimization of extraction technology of Schisandra chinensis polysaccharide and its protective effect on LPS induced mitochondrial membrane potential in macrophages[J]. Science and Technology of Food Industry,2020,41(20):33−40.
    [3]
    QU H M, LIU S J, ZHANG C Y. Antitumor and antiangiogenic activity of Schisandra chinensis polysaccharide in a renal cell carcinoma model[J]. International Journal of Biological Macromolecules,2014,66:52−56. doi: 10.1016/j.ijbiomac.2014.02.025
    [4]
    王珊珊, 李泰雅, 谷舞, 等. 北五味子多糖热水浸提工艺优化及体外抗氧化性能研究[J]. 粮油食品科技,2017,25(1):64−69. [WANG S S, LI T Y, GU W, et al. Optimization of hot water extraction process of Schisandrae chinensis polysaccharide and its in vitro antioxidation property study[J]. Science and Technology of Cereals, Oils and Foods,2017,25(1):64−69. doi: 10.3969/j.issn.1007-7561.2017.01.016
    [5]
    曹丹, 孙于寒, 彭浩, 等. 均匀设计法优化灰树花多糖超声波辅助提取工艺及其抗氧化活性分析[J]. 食品研究与开发,2021,42(5):113−118. [CAO D, SUN Y H, PENG H, et al. Optimization of ultrasonic-assisted extraction process of polysaccharides from Grifola frondosa by uniform design and its antioxidant activity analysis[J]. Food Research and Development,2021,42(5):113−118.
    [6]
    LI S, HAN D, ROW K H. Optimization of enzymatic extraction of polysaccharides from some marine algae by response surface methodology[J]. Korean Journal of Chemical Engineering,2012,29(5):650−656. doi: 10.1007/s11814-011-0221-3
    [7]
    尹秀莲, 游庆红. 超声辅助复合酶法提取桑黄多糖[J]. 食品与机械,2011,27(4):58−60. [YI X L, YOU Q H. Ultrasonic assisted enzymatic extraction of polysaccharides from Phellinus igniarius[J]. Food & Machinery,2011,27(4):58−60. doi: 10.3969/j.issn.1003-5788.2011.04.015
    [8]
    李栋, 薛瑞婷. 响应面耦合遗传算法优化超声辅助复合酶提取红枣多糖工艺[J]. 食品研究与开发,2021,42(6):96−103. [LI D, XUE R T. Optimization of ultrasonic-assisted complex enzyme extraction polysaccharides from jujube by response surface methodology coupled with genetic algorithm[J]. Food Research and Development,2021,42(6):96−103.
    [9]
    XUE H, TAN J, ZHU X, et al. Counter-current fractionation-assisted and bioassay-guided separation of active compounds from cranberry and their interaction with α-glucosidase[J]. LWT-Food Science and Technology,2021,145:111374. doi: 10.1016/j.lwt.2021.111374
    [10]
    韩芬霞, 范新景, 耿升, 等. 异甘草素抑制α-葡萄糖苷酶的分子机制[J]. 食品科学,2019,40(15):37−42. [HAN F X, FAN X J, GENG S, et al. Inhibitory mechanism of isoliquiritigenin against α-glucosidase[J]. Food Science,2019,40(15):37−42. doi: 10.7506/spkx1002-6630-20180830-337
    [11]
    ZENG L, ZHANG G, LIAO Y, et al. Inhibitory mechanism of morin on α-glucosidase and its anti-glycation properties[J]. Food & Function,2016,7(9):3953−396.
    [12]
    谭晓虹, 王治宝, 卫娇娇, 等. 正交试验优化南五味子多糖的超声提取工艺[J]. 神经药理学报,2012,2(6):36−39. [TAN X H, WANG Z B, WEI J J, et al. Optimization of ultrasonic extraction technology of polysaccharide from Schisandra chinensis by orthogonal test[J]. Acta Neuropharmacologica,2012,2(6):36−39.
    [13]
    ZHANG X, BAN Q, WANG X, et al. Green and efficient Peg-based ultrasonic-assisted extraction of polysaccharides from tree peony pods and the evaluation of their antioxidant activity in vitro[J]. Biomed Research International,2018,2018:1−7.
    [14]
    CHEN G, KAN J. Ultrasound-assisted extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Chestnut rose(Rosa roxburghii Tratt) fruit[J]. Journal of Food Science & Technology,2018,55(3):1−10.
    [15]
    BABAMORADI N, YOUSEFI S, ZIARATI P. Optimization of ultrasound-assisted extraction of functional polysaccharides from common mullein(Verbascum thapsus L.) flowers[J]. Journal of Food Process Engineering,2018,41(7):12851.
    [16]
    李刚刚, 于思源, 孙静, 等. 响应面法优化蕨麻多糖超声辅助提取工艺研究[J]. 现代食品,2021,4(1):98−101. [LI G G, YU S Y, SUN J, et al. Response surface methodology to optimize the ultrasonic-assisted extraction process of polysaccharides from Potentilla anserina[J]. Modern Food,2021,4(1):98−101.
    [17]
    ZHONG K, QIANG W. Optimization of ultrasonic extraction of polysaccharides from dried longan pulp using response surface methodology[J]. Carbohydrate Polymers,2010,80(1):19−25. doi: 10.1016/j.carbpol.2009.10.066
    [18]
    吴玉婷, 张慧玉, 刘雅昕, 等. 超声波酶辅助法提取绿萝多糖工艺的研究[J]. 轻工科技,2021,37(4):21−23. [WU Y T, ZHANG H Y, LIU Y X, et al. Study on ultrasonic enzyme assisted extraction of polysaccharide from green pineapple[J]. Journal of Light Industry,2021,37(4):21−23.
    [19]
    LI Y, CHEN X, YAN Y, et al. Optimal extraction technology of polysaccharides from red kindey bean using ultrasonic assistant with enzyme[J]. Transactions of the Chinese Society of Agricultural Engineering,2015,31(15):293−301.
    [20]
    刘日斌, 张宇鹏, 马崇坚, 等. 超声波辅助酶法优化黄精多糖提取工艺的研究[J]. 食品研究与开发,2021,42(7):141−146. [LIU R B, ZHANG Y P, MA C J, et al. Ultrasonic-assisted enzymatic optimization of extraction process of polysaccharides from Polygonatum sibiricum[J]. Food Research and Development,2021,42(7):141−146.
    [21]
    王杉杉, 马韵升, 姚刚, 等. 超声波辅助复合酶法提取枸杞多糖工艺研究[J]. 中国酿造,2015,34(7):134−137. [WANG S S, MA Y S, YAO G, et al. Ultrasound-assisted compound enzyme extraction technology of polysaccharides from Lycium barbarum[J]. China Brewing,2015,34(7):134−137. doi: 10.11882/j.issn.0254-5071.2015.07.032
    [22]
    赵强, 李昭, 杨劝生, 等. 红茂草多糖提取工艺优化及对小鼠免疫功能的影响[J]. 中国酿造,2020,39(8):193−197. [ZHAO Q, LI Z, YANG Q S, et al. Optimization of extraction technology of Dicranostigma leptodum polysaccharides and their effects on immune function of mice[J]. China Brewing,2020,39(8):193−197. doi: 10.11882/j.issn.0254-5071.2020.08.036
    [23]
    LIU Y, GONG G, ZHANG J, et al. Response surface optimization of ultrasound-assisted enzymatic extraction polysaccharides from Lycium barbarum[J]. Carbohydrate Polymers,2014,10:278−284.
    [24]
    王昭珺, 刘晓风, 徐也. 兰州百合芯多糖的提取工艺及其体外抗氧化活性[J]. 现代食品科技,2020,36(9):219−227. [WANG Z J, LIU X F, XU Y. Extraction process of polysaccharides from Lanzhou lily cores and evaluation of their antioxidant activity in vitro[J]. Modern Food Science & Technology,2020,36(9):219−227.
    [25]
    张世奇, 杨娟, 惠永海, 等. 响应面法优化超声波辅助提取辣木叶多糖工艺[J]. 食品研究与开发,2020,41(16):69−74. [ZHANG S Q, YANG J, HUI Y H, et al. Optimization of ultrasonic assisted extraction of polysaccharide from Moringa oleifera leaves by response surface methodology[J]. Food Research and Development,2020,41(16):69−74. doi: 10.12161/j.issn.1005-6521.2020.16.011
    [26]
    冯艳波. 阿拉尔骏枣多糖的提取及其生物活性研究[D]. 阿拉尔: 塔里木大学, 2014.

    MA Y B. Study on extraction and bioactivity of polysaccharide from Alaerjun jujube[D]. Alar: Tarim University, 2014.
    [27]
    XIAO X, JI H, GUI J, et al. Ultrasonic wave extraction of polysaccharide from fruit of Schisandra chinensis and its antioxygenic property to oil[J]. Journal of Northeast Forestry University,2009,37(4):34−36.
    [28]
    ZHANG X, WANG J, XIN Y, et al. Optimization of the extraction process of polysaccharide from Schisandra chinensis(Turcz.) baill[J]. Journal of Physics: Conference Series,2019,1176(6):62032.
    [29]
    冯学珍, 覃慧逢, 冯书珍. 网地藻多糖对α-葡萄糖苷酶体外抑制作用的研究[J]. 食品研究与开发,2020,41(3):35−40. [FENG X Z, QIN H F, FENG S Z, et al. Study on inhibition kinetics of polysaccharide from Dictyota dichotoma on α-glucosidase[J]. Food Research and Development,2020,41(3):35−40.
  • Cited by

    Periodical cited type(3)

    1. 黄青,刘爱荣,张立娟. 顶空气相色谱法测定氢气纳米气泡水中的总氢含量. 环境工程技术学报. 2024(04): 1105-1111 .
    2. 刘慧琴,谢良波,边晖,陈时远,刘淑贞. 顶空气相色谱-荧光技术测定燃料油中硫化氢. 油气田环境保护. 2024(05): 56-61 .
    3. 陈微,王新程,陈远,秦树存. 腹腔注射富氢生理盐水促进高脂血症金黄地鼠胆固醇逆向转运和血脂水平降低. 中国动脉硬化杂志. 2024(12): 1041-1050 .

    Other cited types(4)

Catalog

    Article Metrics

    Article views (308) PDF downloads (30) Cited by(7)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return