Study on activity changes of α- amylase inhibitor from white kidney beans during processing and digestion
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摘要: 本文研究了白芸豆α-淀粉酶抑制剂(α-AI)在加工和消化过程中的活性变化。通过探究α-AI浓度、热、酸、碱以及模拟胃肠道环境对α-AI活性的影响来反映α-AI在加工和消化过程中的活性变化。α-AI对α-淀粉酶的抑制作用在较低蛋白浓度(<6.00 mg/m L)下呈剂量依赖关系,在较高蛋白浓度(>6.00 mg/m L)下随蛋白浓度的增大而略有增强。低于60℃的热处理,α-AI活性无显著变化(p>0.05),高于80℃时α-AI活性严重损失甚至丧失,70℃时α-AI活性有所损失,随着时间的延长活性损失增大;酸碱处理(p H3.010.0)会显著影响α-AI活性(p<0.05),但其对α-AI活性造成的损失会随时间的延长而减小。α-AI活性在消化过程中显著降低(p<0.05),其活性损失主要在胃内;低蛋白浓度(<8.00 mg/m L)的α-AI在体内几乎失活,高蛋白浓度(>10.00 mg/m L)的α-AI可以较好地发挥生理作用。Abstract: Activity changes of α- amylase inhibitor( α- AI) from white kidney beans during processing and digestion were investigated,which were determined after α- AI of different concentrations were treated by heat,acid,alkali and simulating gastrointestinal environment. When α- AI was at lower concentrations( < 6.00 mg / m L),a dose-dependent relationship between the concentration and the activity of α- AI was observed,while α- AI activity increased slightly with the concentration when α- AI was at higher concentrations( > 6.00 mg / m L).Heat treatment under 60 ℃ had no significant influence on α- AI activity( p > 0.05).Heat treatment over 80 ℃ greatly damaged or even destroied α- AI. Heat treatment at 70 ℃ caused an activity loss which increased with the extension of time.Acid and alkali treatment( p H3.0 ~ 10.0) significantly affected the activity of α- AI( p < 0.05),the loss of the activity was decreased with the extension of time.During digestion,α- AI activity suffered from an enormous loss and stomach accounted for the majority of the loss. Low concentration( < 8.00 mg / m L) of α- AI was nearly inactivated. Nonetheless,high concentration( > 10.00 mg / m L) of α- AI could better perform its physiological functions.
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Keywords:
- α-amylase inhibitor /
- food processing /
- activity change
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