Stability and Lipid- lowering Effect of the Complex of <i>Agrocybe Cylindracea</i> Polysaccharides and Pomegranate Peel Polyphenols

QU Wenxin; LIU Rong

doi:10.13386/j.issn1002-0306.2024070036

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QU Wenxin, LIU Rong. Stability and Lipid- lowering Effect of the Complex of Agrocybe Cylindracea Polysaccharides and Pomegranate Peel Polyphenols[J]. Science and Technology of Food Industry, 2025, 46(10): 1−9. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024070036.

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Stability and Lipid- lowering Effect of the Complex of Agrocybe Cylindracea Polysaccharides and Pomegranate Peel Polyphenols

QU Wenxin^1,,
LIU Rong^{1, 2, ,}

1.
College of Life Science Northeast Forestry University, Harbin 150040, China
2.
Key Laboratory of Forest Food Resources utilization of Heilongjiang Province, Harbin 150040, China

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Received Date: July 02, 2024
Available Online: March 18, 2025

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Abstract

Objective: To examine the structure and potential synergistic lipid-lowering effects of the complex of pomegranate peel polyphenols(PPP) and Agrocybe cylindracea polysaccharide(ACP) extracts, as well as the stability of the mixture. Methods: Based on the previous experiments, extracts of Agrocybe cylindracea polysaccharides and pomegranate peel polyphenols were obtained, subjected to quantitative analysis, and non-covalent interaction was used to generate the complexes. The adsorption capacities of the single components and their complexes on saturated fats, unsaturated fats, cholesterol, and pancreatic lipase were assessed using the Chou-Talalay Combined Index (CI) in order to determine whether the two have synergistic lipid-lowering effects. The complexes ACP-PPP were also subjected to structural characterization and stability tests. Results: The primary contacts between the ACP-PPP complexes were revealed by spectroscopic investigations to be hydrogen bonding interactions; no additional double bonds were created during the complex building process. The complex has greater solubility and thermal stability. It was advantageous to preserve the stability of the complex under the conditions of light avoidance, low temperature, and mild acidity. The complex dissociated to varying degrees under varied parameters of light, temperature, pH, storage settings, and digesting system. When ACP was combined with PPP, the amount of saturated fat that was adsorbed was 13±0.14 g/g, the amount of cholesterol that was adsorbed was 74.8±1.23 mg/g, and at a mass concentration of 1.2 mg/mL, the inhibition rate of pancreatic lipase was 57.21±1.32%. Following the complexation of ACP and PPP, the CI value of each assessment index was less than 1, including a good synergistic lipid-lowering ability. Conclusion: Agrocybe cylindracea polysaccharide and pomegranate peel polyphenols have good stability and synergistic hypolipidemic effect after compounding.

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References

[1]	GILES L A. Hyperlipidemia prevention and management utilizing lifestyle changes[J]. Journal of Midwifery & Women's Health,2024,69(3):361−369.
[2]	MURAKAMI, HIRAZAWA C, MIZUTANI T, et al. Edible red seaweed hypnea asiatica ameliorates high-fat diet induced metabolic diseases in mice[J]. Journal of Medicinal Food,2023,26(11):799−808. doi: 10.1089/jmf.2023.K.0010
[3]	ABDUL T, BUKHARI S M A, HERRERA E C, et al. Lipid lowering therapy:an era beyond statins[J]. Current Problems in Cardiology,2022,47(12):101342. doi: 10.1016/j.cpcardiol.2022.101342
[4]	PINAL I, CASAL M, MAMMEN A L. Statins:pros and cons[J]. Medicina Clínica (English Edition),2018,150(10):398−402.
[5]	WANG H, NG T B, LIU Q. Isolation of a new heterodimeric lectin with mitogenic activity from fruiting bodies of the mushroom agrocybe cylindracea[J]. Life Sciences,2002,70(8):877−885. doi: 10.1016/S0024-3205(01)01463-1
[6]	LIU J J, CHEN S K, WANG X, et al. Changes of the physicochemical properties and structural characteristics of alkali-extracted polysaccharides from agrocybe cylindracea across the growth process[J]. Journal of Agricultural and Food Chemistry,2024,72(22):12810−12821. doi: 10.1021/acs.jafc.4c02218
[7]	LIU M, JING H J, ZHANG J J, et al. Optimization of mycelia selenium polysaccharide extraction from agrocybe cylindracea sl-02 and assessment of their antioxidant and anti-ageing activities[J]. Plos One,2016,11(8):e0160799. doi: 10.1371/journal.pone.0160799
[8]	高雅倩, 马诗经, 林丽, 等. 茶树菇多糖提取工艺优化及抗氧化活性研究[J]. 现代食品,2022,28(1):200−204. [GAO Y Q, MA S J, LIN L, et al. Optimisation of the extraction process and antioxidant activity of polysaccharides from tea tree mushroom[J]. Modern Food,2022,28(1):200−204.] GAO Y Q, MA S J, LIN L, et al. Optimisation of the extraction process and antioxidant activity of polysaccharides from tea tree mushroom[J]. Modern Food, 2022, 28（1）: 200−204.
[9]	徐峻, 方绍海, 辛英姬, 等. 应用茶树菇(As-1)多糖评价小鼠高脂模型的建立[J]. 安徽农业科学,2010,38(29):16248−16249. [XU J, FANG S H, XIN Y J, et al. Application of tea tree mushroom (as-1) polysaccharide to evaluate the establishment of a mouse hyperlipidemic model[J]. Journal of Anhui Agricultural Sciences,2010,38(29):16248−16249.] XU J, FANG S H, XIN Y J, et al. Application of tea tree mushroom （as-1） polysaccharide to evaluate the establishment of a mouse hyperlipidemic model[J]. Journal of Anhui Agricultural Sciences, 2010, 38（29）: 16248−16249.
[10]	SINGH J, KAUR H P, VERMA A, et al. Pomegranate peel phytochemistry, pharmacological properties, methods of extraction, and its application:a comprehensive review[J]. ACS omega,2023,8(39):35452−35469. doi: 10.1021/acsomega.3c02586
[11]	闫晓越, 王子琦, 王桂林, 等. 石榴皮多酚提取研究进展[J]. 山东化工,2022,51(24):79−80,83. [YAN X Y, WANG Z Q, WANG G L, et al. Progress of pomegranate peel polyphenol extraction[J]. Shandong Chemical Industry,2022,51(24):79−80,83.] YAN X Y, WANG Z Q, WANG G L, et al. Progress of pomegranate peel polyphenol extraction[J]. Shandong Chemical Industry, 2022, 51（24）: 79−80,83.
[12]	ZHAO W, LI J, HE X Y, et al. In vitro steatosis hepatic cell model to compare the lipid-lowering effects of pomegranate peel polyphenols with several other plant polyphenols as well as its related cholesterol efflux mechanisms[J]. Toxicology Reports,2014,1:945−954. doi: 10.1016/j.toxrep.2014.10.013
[13]	殷伟伟, 张松. 食药用真菌降血脂作用的研究及应用[J]. 菌物研究,2006,4(4):5. [YIN W W, ZHANG S. Study and application of hypolipidemic effect of edible and medicinal fungi[J]. Journal of Fungal Research,2006,4(4):5.] YIN W W, ZHANG S. Study and application of hypolipidemic effect of edible and medicinal fungi[J]. Journal of Fungal Research, 2006, 4（4）: 5.
[14]	王素敏, 徐欢欢, 黄业伟, 等. 茶多酚的降脂作用及其机制研究进展[J]. 食品研究与开发,2016,37(10):219−224. [WANG S M, XU H H, HUANG W Y, et al. Progress in the study of lipid-lowering effect of tea polyphenols and its mechanism[J]. Food Research and Development,2016,37(10):219−224.] WANG S M, XU H H, HUANG W Y, et al. Progress in the study of lipid-lowering effect of tea polyphenols and its mechanism[J]. Food Research and Development, 2016, 37（10）: 219−224.
[15]	ZHU J F, ZHANG D X, TANG H Y, et al. Structure relationship of non–covalent interactions between phenolic acids and arabinan–rich pectic polysaccharides from rapeseed meal[J]. International Journal of Biological Macromolecules,2018,120:2597−2603. doi: 10.1016/j.ijbiomac.2018.09.036
[16]	KE C X , LI L. Modification mechanism of soybean protein isolate-soluble soy polysaccharide complex by egcg through covalent and non-covalent interaction:structural, interfacial, and functional properties[J]. Food Chemistry, 2024, 448:139033.
[17]	崔灵敏, 谢笔钧, 孙智达. 果胶与莲原花青素复合物理化性质及体外抑菌活性研究[J]. 食品工业科技,2020,41(16):60−66,80. [CUI L M, XIE B J, SUN Z D. Physicochemical properties and in vitro antibacterial activity of pectin complexed with lotus vulgaris anthocyanin[J]. Science and Technology of Food Industry,2020,41(16):60−66,80.] CUI L M, XIE B J, SUN Z D. Physicochemical properties and in vitro antibacterial activity of pectin complexed with lotus vulgaris anthocyanin[J]. Science and Technology of Food Industry, 2020, 41（16）: 60−66,80.
[18]	LI Z D, XIAO W H, XIE J H, et al. Isolation, characterization and antioxidant activity of yam polysaccharides[J]. Foods,2022,11(6):800. doi: 10.3390/foods11060800
[19]	ZHAO X, FU Z, YAO M H, et al. Mulberry leaf polysaccharide ameliorates insulin resistance and a dipose deposition associated gut microbiota and lipid metabolites in high fat diet induced obese mice[J]. Food Science & Nutrition,2022,10(2):617−630.
[20]	胡晓倩, 唐洪华, 程安阳. 茶树菇多糖提取及其抗氧化性能的研究[J]. 湖北农业科学,2011,50(21):4465−4468. [HU X Q, TANG H H, CHENG A Y. Extraction of polysaccharides from tea tree mushroom and its antioxidant properties[J]. Hubei Agricultural Sciences,2011,50(21):4465−4468.] doi: 10.3969/j.issn.0439-8114.2011.21.047 HU X Q, TANG H H, CHENG A Y. Extraction of polysaccharides from tea tree mushroom and its antioxidant properties[J]. Hubei Agricultural Sciences, 2011, 50（21）: 4465−4468. doi: 10.3969/j.issn.0439-8114.2011.21.047
[21]	左栖枫, 张新明, 邓舒畅, 等. S-8大孔树脂纯化会理石榴皮中多酚的工艺研究[J]. 轻工科技,2021,37(02):15−18. [ZUO Q F, ZHANG X M, DENG S C, et al. Process study on the purification of polyphenols from pomegranate peel in huili with s-8 macroporous resin[J]. Light Industrial Science and Technology,2021,37(02):15−18.] ZUO Q F, ZHANG X M, DENG S C, et al. Process study on the purification of polyphenols from pomegranate peel in huili with s-8 macroporous resin[J]. Light Industrial Science and Technology, 2021, 37（02）: 15−18.
[22]	LI S, LI J S, ZHU Z Z, et al. Soluble dietary fiber and polyphenol complex in lotus root:preparation, interaction and identification[J]. Food Chemistry,2020,314:126219. doi: 10.1016/j.foodchem.2020.126219
[23]	陈燕云, 平华, 高媛, 等. 黍稷麸皮多糖分离纯化、结构表征及抑菌活性分析[J]. 食品工业科技,2024,45(10):1−7. [CHEN Y Y, P H, G Y, et al. Isolation and purification of polysaccharides from millet bran, structural characterisation and analysis of antibacterial activities[J]. Science and Technology of Food Industry,2024,45(10):1−7.] CHEN Y Y, P H, G Y, et al. Isolation and purification of polysaccharides from millet bran, structural characterisation and analysis of antibacterial activities[J]. Science and Technology of Food Industry, 2024, 45（10）: 1−7.
[24]	羡荣华, 蒲铎文, 樊梓鸾, 等. 老山芹多糖的分离纯化、结构表征及体外降糖活性研究[J]. 食品与发酵工业,2023,49(18):113−118,124. [XIAN R H, PU D W, FAN Z L, et al. Isolation and purification, structural characterisation and in vitro hypoglycemic activity of polysaccharides from cynodon grandiflorus[J]. Food and Fermentation Industries,2023,49(18):113−118,124.] XIAN R H, PU D W, FAN Z L, et al. Isolation and purification, structural characterisation and in vitro hypoglycemic activity of polysaccharides from cynodon grandiflorus[J]. Food and Fermentation Industries, 2023, 49（18）: 113−118,124.
[25]	邢慧珍, 张玉梅, 刘会平, 等. 淡竹叶多糖的制备、热稳定性及其抗氧化活性[J]. 食品研究与开发,2023,44(19):86−96. [XING H Z, ZHANG Y M, LIU H P, et al. Preparation, thermal stability and antioxidant activity of polysaccharides from tempranillo leaves[J]. Food Research and Development,2023,44(19):86−96.] XING H Z, ZHANG Y M, LIU H P, et al. Preparation, thermal stability and antioxidant activity of polysaccharides from tempranillo leaves[J]. Food Research and Development, 2023, 44（19）: 86−96.
[26]	马婷, 曲航, 杨海龙. 提取方法对羊栖菜多糖理化性质及体外生物活性的影响[J]. 食品安全质量检测学报,2023,14(15):259−268. [MA T, QU H, YANG H L. Influence of extraction methods on the physicochemical properties and in vitro bioactivity of polysaccharides from lamb's quarters[J]. Journal of Food Safety & Quality,2023,14(15):259−268.] MA T, QU H, YANG H L. Influence of extraction methods on the physicochemical properties and in vitro bioactivity of polysaccharides from lamb's quarters[J]. Journal of Food Safety & Quality, 2023, 14（15）: 259−268.
[27]	刘秋兰, 邹晓琴, 易阳, 等. 莲藕多糖和阿魏酸的非共价相互作用及其复合物特性[J]. 中国食品学报,2023,23(7):26−36. [LIU Q L, ZOU X Q, YI Y, et al. Non-covalent interactions between lotus root polysaccharides and ferulic acid and their complex properties[J]. Journal of Chinese Institute of Food Science and Technology,2023,23(7):26−36.] LIU Q L, ZOU X Q, YI Y, et al. Non-covalent interactions between lotus root polysaccharides and ferulic acid and their complex properties[J]. Journal of Chinese Institute of Food Science and Technology, 2023, 23（7）: 26−36.
[28]	苏宁, 刘志明, 王海英. 桑叶多酚提取工艺优化及复配物比较研究[J]. 食品科技,2024,49(5):178−186. [SU N, LIU Z M, WANG H Y. Optimisation of mulberry leaf polyphenol extraction process and comparative study of compounds[J]. Food Science and Technology,2024,49(5):178−186.] SU N, LIU Z M, WANG H Y. Optimisation of mulberry leaf polyphenol extraction process and comparative study of compounds[J]. Food Science and Technology, 2024, 49（5）: 178−186.
[29]	何袅袅, 蔡树芸, 阎光宇, 等. 铜藻多酚的提取工艺及其稳定性和抑制酪氨酸酶活性[J]. 食品研究与开发,2024,45(9):104−110. [HE N N, CAI S Y, YAN G Y, et al. Extraction process of polyphenols from copper algae and their stability and tyrosinase inhibitory activity[J]. Food Research and Development,2024,45(9):104−110.] doi: 10.12161/j.issn.1005-6521.2024.09.015 HE N N, CAI S Y, YAN G Y, et al. Extraction process of polyphenols from copper algae and their stability and tyrosinase inhibitory activity[J]. Food Research and Development, 2024, 45（9）: 104−110. doi: 10.12161/j.issn.1005-6521.2024.09.015
[30]	穆谈航, 叶嘉, 杨明建, 等. 石榴皮多酚提取工艺优化及其贮藏稳定性研究[J]. 食品工业科技,2021,42(11):142−146. [MU T H, YE J, YANG M J, et al. Optimisation of pomegranate peel polyphenol extraction process and its storage stability studies[J]. Science and Technology of Food Industry,2021,42(11):142−146.] MU T H, YE J, YANG M J, et al. Optimisation of pomegranate peel polyphenol extraction process and its storage stability studies[J]. Science and Technology of Food Industry, 2021, 42（11）: 142−146.
[31]	雷丹, 李军胜, 李书艺, 等. 莲藕可溶性膳食纤维与多酚复合物的稳定性及脂肪吸附活性研究[J]. 中国食品学报,2022,22(2):31−39. [LEI D, LI S J, LI S Y, et al. Studies on the stability and fat adsorption activity of soluble dietary fibre and polyphenol complexes from lotus roots[J]. Journal of Chinese Institute of Food Science and Technology,2022,22(2):31−39.] LEI D, LI S J, LI S Y, et al. Studies on the stability and fat adsorption activity of soluble dietary fibre and polyphenol complexes from lotus roots[J]. Journal of Chinese Institute of Food Science and Technology, 2022, 22（2）: 31−39.
[32]	LI X G, ZHANG F Y, JIANG C X, et al. Structural analysis, in vitro antioxidant and lipid-lowering activities of purified tremella fuciformis polysaccharide fractions[J]. Process Biochemistry,2023,133:99−108. doi: 10.1016/j.procbio.2023.06.005
[33]	王威振, 杨盼盼, 遆永瑞, 等. 龙牙百合多糖的超声辅助提取及其抗氧化、降血脂活性分析[J]. 食品工业科技,2023,44(18):251−257. [WANG W Z, YANG P P, TI Y R, et al. Ultrasound-assisted extraction of polysaccharides from longya lilies and analysis of their antioxidant and hypolipidemic activities[J]. Science and Technology of Food Industry,2023,44(18):251−257.] WANG W Z, YANG P P, TI Y R, et al. Ultrasound-assisted extraction of polysaccharides from longya lilies and analysis of their antioxidant and hypolipidemic activities[J]. Science and Technology of Food Industry, 2023, 44（18）: 251−257.
[34]	曹艳妮, 徐坤, 邱思, 等. 刺梨、苹果和脐橙复配物的抗氧化能力[J]. 食品研究与开发,2023,44(7):60−66. [CAO Y N, XU K, QIU S, et al. Antioxidant capacity of prickly pear, apple and navel orange complexes[J]. Food Research and Development,2023,44(7):60−66.] doi: 10.12161/j.issn.1005-6521.2023.07.009 CAO Y N, XU K, QIU S, et al. Antioxidant capacity of prickly pear, apple and navel orange complexes[J]. Food Research and Development, 2023, 44（7）: 60−66. doi: 10.12161/j.issn.1005-6521.2023.07.009
[35]	WANG J Y, LIU W, CHEN Z Q, et al. Physicochemical characterization of the oolong tea polysaccharides with high molecular weight and their synergistic effects in combination with polyphenols on hepatocellular carcinoma[J]. Biomedicine & Pharmacotherapy,2017,90:160−170.
[36]	Košťálová Z, Hromádková Z. Structural characterisation of polysaccharides from roasted hazelnut skins[J]. Food Chemistry,2019,286:179−184. doi: 10.1016/j.foodchem.2019.01.203
[37]	FAN H R, YAO X, CHEN Z J, et al. Interaction of high amylose corn starch with polyphenols:Modulating the stability of polyphenols with different structure against thermal processing[J]. Food Chemistry,2024,437:137708. doi: 10.1016/j.foodchem.2023.137708
[38]	BUCHWEITZ M, CARLE R, KAMMERER D R. Bathochromic and stabilising effects of sugar beet pectin and an isolated pectin fraction on anthocyanins exhibiting pyrogallol and catechol moieties[J]. Food Chemistry,2012,135(4):3010−3019. doi: 10.1016/j.foodchem.2012.06.101
[39]	ZHANG X J, KONG X R, HAO Y, et al. Chemical structure and inhibition on α-glucosidase of polysaccharide with alkaline-extracted from glycyrrhiza inflata residue[J]. International Journal of Biological Macromolecules,2020,147:1125−1135. doi: 10.1016/j.ijbiomac.2019.10.081
[40]	邹晓琴, 刘秋兰, 黄菲, 等. 莲藕多糖-绿原酸复合体制备及特性评价[J]. 现代食品科技,2023,39(7):184−193. [ZOU X Q, LIU Q L, HUANG F, et al. Preparation and characterisation of lotus root polysaccharide-chlorogenic acid complexes[J]. Modern Food Science and Technology,2023,39(7):184−193.] ZOU X Q, LIU Q L, HUANG F, et al. Preparation and characterisation of lotus root polysaccharide-chlorogenic acid complexes[J]. Modern Food Science and Technology, 2023, 39（7）: 184−193.
[41]	张阳洋, 范金波, 麻奥, 等. 分子模拟结合荧光光谱法探究BSA-多酚复合物形成机理及对多酚的保护作用[J]. 中国食品学报,2021,21(8):298−308. [ZHANG Y Y, FAN J B, MA A, et al. Molecular modelling combined with fluorescence spectroscopy to investigate the formation mechanism of bsa-polyphenol complexes and their protective effects on polyphenols[J]. Journal of Chinese Institute of Food Science and Technology,2021,21(8):298−308.] ZHANG Y Y, FAN J B, MA A, et al. Molecular modelling combined with fluorescence spectroscopy to investigate the formation mechanism of bsa-polyphenol complexes and their protective effects on polyphenols[J]. Journal of Chinese Institute of Food Science and Technology, 2021, 21（8）: 298−308.
[42]	干建松. 荸荠皮多酚的纯化与抗氧化性、稳定性研究[J]. 食品研究与开发,2022,43(17):27−33. [GAN J S. Purification and antioxidant properties and stability of polyphenols from water chestnut skin[J]. Food Research and Development,2022,43(17):27−33.] doi: 10.12161/j.issn.1005-6521.2022.17.004 GAN J S. Purification and antioxidant properties and stability of polyphenols from water chestnut skin[J]. Food Research and Development, 2022, 43（17）: 27−33. doi: 10.12161/j.issn.1005-6521.2022.17.004
[43]	SALAZAR G L, VILLALBA R, BERNAL M J, et al. Effect of storage conditions on the stability of polyphenols of apple and strawberry purees produced at industrial scale by different processing techniques[J]. Journal of Agricultural and Food Chemistry,2023,71(5):2541−2553. doi: 10.1021/acs.jafc.2c07828
[44]	魏奇, 翁馨, 吴艳钦, 等. 食用菌多糖降脂活性及其作用机制的研究进展[J]. 食品科技,2021,46(9):190−194. [WEI Q, WENG X, WU Y Q, et al. Research progress on lipid-lowering activity of edible mushroom polysaccharides and their mechanism of action[J]. Food Science and Technology,2021,46(9):190−194.] WEI Q, WENG X, WU Y Q, et al. Research progress on lipid-lowering activity of edible mushroom polysaccharides and their mechanism of action[J]. Food Science and Technology, 2021, 46（9）: 190−194.
[45]	施慧, 张玉, 陈磊, 等. 龙眼壳/核膳食纤维对油脂、胆固醇和胆酸钠的吸附作用[J]. 食品工业,2015,36(3):103−107. [SHI H, ZHANG Y, CHEN L, et al. Adsorption of lipids, cholesterol and sodium bile acids by longan shell/kernel dietary fibre[J]. The Food Industry,2015,36(3):103−107.] SHI H, ZHANG Y, CHEN L, et al. Adsorption of lipids, cholesterol and sodium bile acids by longan shell/kernel dietary fibre[J]. The Food Industry, 2015, 36（3）: 103−107.
[46]	钟希琼, 胡文娥, 林丽超. 膳食纤维对油脂、胆固醇、胆酸钠和亚硝酸根离子吸附作用的研究[J]. 食品工业科技,2010,31(5):134−136. [ZHONG X Q, HU W E, LIN L C. Studies on the adsorption of lipids, cholesterol, sodium cholate and nitrite ions by dietary fibre[J]. Science and Technology of Food Industry,2010,31(5):134−136.] ZHONG X Q, HU W E, LIN L C. Studies on the adsorption of lipids, cholesterol, sodium cholate and nitrite ions by dietary fibre[J]. Science and Technology of Food Industry, 2010, 31（5）: 134−136.
[47]	YIN D F, ZHONG Y D, LIU H, et al. Lipid metabolism regulation by dietary polysaccharides with different structural properties[J]. International Journal of Biological Macromolecules, 2024:132253.
[48]	SUN H R, FAN R Q, FANG R, et al. Dynamics changes in metabolites and pancreatic lipase inhibitory ability of instant dark tea during liquid-state fermentation by aspergillus niger[J]. Food Chemistry,2024,448:139136. doi: 10.1016/j.foodchem.2024.139136

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[5]	LIN Shan, ZHAO Ping, FU Yunna, LIU Bing, FENG Peiyao, LI Haozhe, XIA Xingxing. Optimization of Liquid Fermentation Medium Formulation of Morchella eohespera Exopolysaccharide and Its Hypoglycemic Activity in Vitro[J]. Science and Technology of Food Industry, 2022, 43(20): 196-203. DOI: 10.13386/j.issn1002-0306.2021120191
[6]	GUAN Lijun, LI Jialei, WANG Kunlun, GAO Yang, YAN Song, CHEN Kaixin, JI Nina, LI Bo, WANG Jiayou, LU Shuwen. Effects of Lactic Acid Bacteria Fermentation on the Active Components, Antioxidant Activity and Enzymes on Hypoglycemic Activity in Vitro of Acanthopanax senticosus Leaves[J]. Science and Technology of Food Industry, 2022, 43(7): 155-162. DOI: 10.13386/j.issn1002-0306.2021080265
[7]	WANG Meng-ya, ZHAO Zhe-zhen, XUE Jiao, HU Jin-rong, ZHANG Jing-sheng, LIU Ping. Hypoglycemic Activity of Purified Polysaccharides from Inonotus obliquus in Vitro[J]. Science and Technology of Food Industry, 2020, 41(10): 316-320,326. DOI: 10.13386/j.issn1002-0306.2020.10.053
[8]	LUO Jia-yuan, SUN Kai-feng, BAO Yi-hong. Optimization of Enzymatic Biotransformation Process of Polysaccharides from Auricularia auricula and Its Hypoglycemic Performance in Vitro[J]. Science and Technology of Food Industry, 2019, 40(21): 203-209,230. DOI: 10.13386/j.issn1002-0306.2019.21.033
[9]	FENG Yan-bo, BAO Yi-hong. Effect of ultrafine grinding on hypoglycemic and hypolipidemic functional properties in vitro of dietary fiber from pine residue[J]. Science and Technology of Food Industry, 2016, (23): 342-346. DOI: 10.13386/j.issn1002-0306.2016.23.056
[10]	YE Qiong-xian, LIU Jing, MIAO Ai-qing, WANG Dong-mei. Study on antioxidant and in vitro hypoglycemia activities of dark tea prepared from Camellia sinensis var. Baiye Dancong[J]. Science and Technology of Food Industry, 2014, (16): 153-157. DOI: 10.13386/j.issn1002-0306.2014.16.026