Optimization of Polysaccharides Extraction from the Peels of <i>Akebia trifoliate</i> and Evaluation of Its <i>in Vitro</i> Antioxidant and Hypoglycemic Activity

LIU Yongling; LIU Xianglian; TAO Xin; YANG Wei; XU Su

doi:10.13386/j.issn1002-0306.2024010091

Volume 45 Issue 19

Sep. 2024

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Science and Technology of Food Industry > 2024 > 45(19): 17-24. > DOI: 10.13386/j.issn1002-0306.2024010091

LIU Yongling, LIU Xianglian, TAO Xin, et al. Optimization of Polysaccharides Extraction from the Peels of Akebia trifoliate and Evaluation of Its in Vitro Antioxidant and Hypoglycemic Activity[J]. Science and Technology of Food Industry, 2024, 45(19): 17−24. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024010091.

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Optimization of Polysaccharides Extraction from the Peels of Akebia trifoliate and Evaluation of Its in Vitro Antioxidant and Hypoglycemic Activity

LIU Yongling^1,,
LIU Xianglian¹,
TAO Xin¹,
YANG Wei¹,
XU Su^{1, 2, ,}

1.
College of Food Science and Engineering, Guiyang University, Guiyang 550005, China
2.
Guizhou Engineering Research Center for Characteristic Flavor Perception and Quality Control of Drug-Food Homologous Resources, Guiyang 550005, China

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Received Date: January 09, 2024
Available Online: July 29, 2024

Graphical Abstract

Abstract

Abstract

The aim of this research was to optimize the polysaccharides extraction process from the peels of Akebia trifoliate, and evaluate its in vitro antioxidant and hypoglycemic activity. The ultrasonic-assisted extraction process of polysaccharides from the peels of Akebia trifoliate was optimized using single-factor combined with orthogonal experiment. The antioxidant activity of polysaccharides from the peels of Akebia trifoliate was investigated by using DPPH radical scavenging rate and total reducing ability as the indicators. The hypoglycemic activity of polysaccharides from the peels of Akebia trifoliate was explored using α-glucosidase and α-amylase inhibition rate as the indicators. The results demonstrated that the optimal ultrasonic-assisted extraction process of polysaccharides from the peels of Akebia trifoliate was ultrasound power 280 W, ultrasound time 60 min, extraction temperature 50 ℃, solid-liquid ratio 1:40 g/mL, respectively. Under the optimal process condition, the extraction rate of polysaccharides from the peels of Akebia trifoliate was 5.91%±0.20%. Within the measured concentration range, polysaccharides from the peels of Akebia trifoliate had the preferable DPPH radical scavenging and reducing ability. In addition, the IC₅₀ values of polysaccharides from the peels of Akebia trifoliate and V_C for scavenging DPPH free radicals were 0.329 and 0.077 mg/mL, separately. The IC₅₀ values of polysaccharides from the peels of Akebia trifoliate for inhibiting α-glucosidase and α-amylase activity were 0.386 and 0.307 mg/mL, respectively. This research proved that the polysaccharides from the peels of Akebia trifoliate extracted by this optimal process had better in vitro antioxidant and hypoglycemic activity.
- Akebia trifoliate peels,
- polysaccharides,
- extraction process,
- antioxidant,
- hypoglycemic

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