Purification and Component Analysis of Total Flavonoids from Sea Buckthorn Peel Residue and Its Anti-Pancreatic Lipase

SUN Jiali; JIANG Junfeng; MOU Xiaojuan; ZONG Zehui; YAN Siyu; JIALALIDING·Remuzha; BAO Xiaowei

doi:10.13386/j.issn1002-0306.2023110077

Volume 45 Issue 19

Sep. 2024

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Science and Technology of Food Industry > 2024 > 45(19): 206-215. > DOI: 10.13386/j.issn1002-0306.2023110077

SUN Jiali, JIANG Junfeng, MOU Xiaojuan, et al. Purification and Component Analysis of Total Flavonoids from Sea Buckthorn Peel Residue and Its Anti-Pancreatic Lipase[J]. Science and Technology of Food Industry, 2024, 45(19): 206−215. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110077.

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Purification and Component Analysis of Total Flavonoids from Sea Buckthorn Peel Residue and Its Anti-Pancreatic Lipase

1.
School of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China
2.
Department of Grain, Oil and Food Engineering, Xinjiang Institute of Technology and Economics,Urumqi 830013, China

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Received Date: November 08, 2023
Available Online: September 10, 2024

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Abstract

Abstract

In order to clarify the inhibition of total flavonoids purified extract (TFSE) of sea buckthorn peel residue on pancreatic lipase, this research optimized the purification condition of total flavonoids crude extract (TFCE) of sea buckthorn peel residue using AB-8 macroporous resin by means of static and dynamic adsorption and desorption experiment. Ultra-performance liquid chromatography-high resolution mass spectrometry (UPLC-Q-MS) was used to analyze the component of TFSE. In addition, the inhibitory effect and types of TFSE on pancreatic lipase were explored through the method of nitrophenyl butyrate combined with enzymatic reaction kinetics. The results showed that the optimal purification conditions for AB-8 macroporous resin were a sample concentration of 2.2 mg/mL, a sample pH of 5.0, a sample volume of 140 mL, and a sample flow rate of 1.0 mL/min. The volume fraction of ethanol was 75% with the eluent being 120 mL and the elution flow rate being 1.5 mL/min. After separation and purification under these conditions, the purity of TFSE increased from 9.786% to 50.204%. The total of 39 phenolic compounds were identified from TFSE with flavonoids like rutin, catechins, and quercetin-3-O-glucoside being the main compounds. TFSE had a strong inhibition on pancreatic lipase with the IC₅₀ being 12.46 mg/mL. In addition, the inhibition type was determined to be non-competitive inhibition by using double reciprocal plot method of Lineweaver-Burk with an inhibition constant K_i being 10.21 mg/mL. TFSE has better inhibitory activity on pancreatic lipase, which is of great significance for improving the added value of sea buckthorn peel residue products.

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