Purification and Characterization of Buckwheat Bee Pollen Polysaccharide and Changes of Its Antioxidant Activity during <i>in Vitro</i> Digestion

WANG Hua; MENG Jingjing; MA Yunxiao; ZHANG Zhiwei; YU Lili; ZHANG Jixing; ZHOU Wenxi

doi:10.13386/j.issn1002-0306.2023110040

Volume 45 Issue 19

Sep. 2024

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Science and Technology of Food Industry > 2024 > 45(19): 58-65. > DOI: 10.13386/j.issn1002-0306.2023110040

WANG Hua, MENG Jingjing, MA Yunxiao, et al. Purification and Characterization of Buckwheat Bee Pollen Polysaccharide and Changes of Its Antioxidant Activity during in Vitro Digestion[J]. Science and Technology of Food Industry, 2024, 45(19): 58−65. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023110040.

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Purification and Characterization of Buckwheat Bee Pollen Polysaccharide and Changes of Its Antioxidant Activity during in Vitro Digestion

WANG Hua^{1, 2,},
MENG Jingjing¹,
MA Yunxiao¹,
ZHANG Zhiwei¹,
YU Lili¹,
ZHANG Jixing^{1, 2},
ZHOU Wenxi^{2, 3, ,}

1.
College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China
2.
Buckwheat Industry Engineering Research Center of Inner Mongolia Minzu University, Tongliao 028000, China
3.
Tongliao Institute of Agriculture and Animal Husbandry Science, Tongliao 028015, China

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Received Date: November 07, 2023
Available Online: July 31, 2024

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Abstract

Abstract

This paper aimed to investigate the antioxidant activity of buckwheat bee pollen polysaccharides (BWPP) during the in vitro gastrointestinal digestion. The crude polysaccharides were purified using DEAE-52 cellulose column and their antioxidant activity during in vitro simulated gastric and intestinal digestion was studied. The results showed that after purification by DEAE-52 cellulose column chromatography, the highest total sugar content component BWPP-1 was obtained, which was mainly composed of Glc, Xyl, Ara, etc. The infrared spectrum showed that BWPP-1 had obvious polysaccharide characteristic absorption peaks. During the simulated in vitro gastrointestinal digestion, the ABTS⁺· scavenging rates of BWPP and BWPP-1 reached their maximum values at 60 minutes of simulated gastric juice digestion, which were 86.39%±1.28% and 90.38%±2.78%, respectively. There was a significant difference compared to other digestion times (P<0.05). The DPPH· clearance rates reached their maximum values at 60 minutes, which were 86.86%±0.11% and 89.58%±1.67%, respectively, and the difference between the two was not significant (P>0.05). When simulating intestinal digestion, the maximum clearance rates of ABTS⁺· by BWPP and BWPP-1 reached 88.67%±0.40% and 91.82%±2.77% at 60 minutes, and the difference between the two was not significant (P>0.05). The DPPH· scavenging rates reached their peak values at 240 minutes of 57.11%±0.06% and 65.67%±3.67%, with significant difference (P<0.05). During the digestion process, the ability to reduce iron ions was relatively weak. Based on these findings, it can be concluded that BWPP and BWPP-1 have good ABTS⁺· and DPPH· scavenging abilities, and the DPPH· scavenging ability during gastric digestion is slightly higher than that during intestinal digestion, while the ABTS⁺· scavenging ability during intestinal digestion is slightly higher than that during gastric digestion. This study provides a theoretical basis for the development of functional products based on buckwheat bee pollen.
- buckwheat bee pollen,
- polysaccharide,
- purification,
- in vitro simulated digestion,
- antioxidant activity

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References

[1]	FEDERICA P, SILVIA G, MARCAZZAN G L, et al. Buckwheat honeys:Screening of composition and properties[J]. Food Chemistry,2013,141:2802−2811. doi: 10.1016/j.foodchem.2013.05.102
[2]	FERREIRA I C F R, HELENO S A, REIS F S, et al. Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities[J]. Phytochemistry,2015,114:38−55. doi: 10.1016/j.phytochem.2014.10.011
[3]	任明. 复合多糖抗辐射和抗肿瘤作用及其机制研究[D]. 长春:吉林大学, 2014. [REN Ming. Anti-tumor and anti-radiation effects of the compound polysaccharide and their mechanisms[D]. Changchun:Jilin University, 2014.] REN Ming. Anti-tumor and anti-radiation effects of the compound polysaccharide and their mechanisms[D]. Changchun: Jilin University, 2014.
[4]	LI Xinzhi, GONG Haiquan, YANG Siwen, et al. Pectic bee pollen polysaccharide from Rosa rugosa alleviates diet-induced hepatic steatosis and insulin resistance via induction of AMPK/mTOR-mediated autophagy[J]. Molecules,2017,22(5):699−712. doi: 10.3390/molecules22050699
[5]	李娟. 荞麦蜂花粉多糖对肠道菌群的影响[D]. 南昌:南昌大学, 2020. [LI Juan. Effects of polysaccharide from Fagopyrum esculentum Moench bee pollen on instinal microlaora[D]. Nanchang:Nanchang University, 2020.] LI Juan. Effects of polysaccharide from Fagopyrum esculentum Moench bee pollen on instinal microlaora[D]. Nanchang: Nanchang University, 2020.
[6]	王胤康. 青钱柳活性成分降血糖作用研究[D]. 武汉:华中科技大学, 2016. [WANG Yinkang. Study on the effects and mechanisms of Cyclocarya paliurus (Batal.) Iljinskaja leaves extracts on hyperglycemia[D]. Wuhan:Huazhong University of Science and Technology, 2016.] WANG Yinkang. Study on the effects and mechanisms of Cyclocarya paliurus （Batal.） Iljinskaja leaves extracts on hyperglycemia[D]. Wuhan: Huazhong University of Science and Technology, 2016.
[7]	林春榕, 张翠香, 左绍远, 等. 荞麦蜂花粉多糖对糖尿病大鼠血糖、血脂的影响[J]. 亚太传统医药,2013,9(3):7−9. [LIN Chunrong, ZHANG Cuixiang, ZUO Shaoyuan, et al. Effect of polysaccharide from buckwheat bee pollen on blood glucose and lipids in experimental diabetic rats[J]. Asia-pacific Traditional Medicine,2013,9(3):7−9.] doi: 10.3969/j.issn.1673-2197.2013.03.004 LIN Chunrong, ZHANG Cuixiang, ZUO Shaoyuan, et al. Effect of polysaccharide from buckwheat bee pollen on blood glucose and lipids in experimental diabetic rats[J]. Asia-pacific Traditional Medicine, 2013, 9（3）: 7−9. doi: 10.3969/j.issn.1673-2197.2013.03.004
[8]	钱明辉, 达热卓玛, 徐德平. 荞麦蜂花粉多糖A的分离及其降血糖活性[J]. 食品与生物技术学报,2019,38(6):80−85. [QIAN Minghui, DARE Dolma, XU Deping. Separation of polysaccharide A from buckwheat bee pollen and its hypoglycemic activity[J]. Journal of Food Science and Biotechnology,2019,38(6):80−85.] doi: 10.3969/j.issn.1673-1689.2019.06.011 QIAN Minghui, DARE Dolma, XU Deping. Separation of polysaccharide A from buckwheat bee pollen and its hypoglycemic activity[J]. Journal of Food Science and Biotechnology, 2019, 38（6）: 80−85. doi: 10.3969/j.issn.1673-1689.2019.06.011
[9]	李娟, 邓泽元, 范亚苇. 荞麦蜂花粉多糖对人体粪便菌群代谢的影响[J]. 中国食品学报,2021,21(5):133−139. [LI Juan, DENG Zeyuan, FAN Yawei. Effects of polysaccharide from Fagopyrum esculentum Moench bee pollen on metabolism of human fecal flora[J]. Journal of Chinese Institute of Food Science and Technology,2021,21(5):133−139.] LI Juan, DENG Zeyuan, FAN Yawei. Effects of polysaccharide from Fagopyrum esculentum Moench bee pollen on metabolism of human fecal flora[J]. Journal of Chinese Institute of Food Science and Technology, 2021, 21（5）: 133−139.
[10]	张萍, 陈燕, 王晓玲. 荞麦花粉多糖的提取工艺及抗氧化性能研究[J]. 食品科技,2011,36(7):169−173. [ZHANG Ping, CHEN Yan, WANG Xiaoling. The extracting technology and antioxidation of polysaccharides from buckwheat bee pollen[J]. Food Science and Technology,2011,36(7):169−173.] ZHANG Ping, CHEN Yan, WANG Xiaoling. The extracting technology and antioxidation of polysaccharides from buckwheat bee pollen[J]. Food Science and Technology, 2011, 36（7）: 169−173.
[11]	SHI Lei. Bioactivities, isolation and purification methods of polysaccharides from natural products:A review[J]. International Journal of Biological Macromolecules,2016,92:37−48. doi: 10.1016/j.ijbiomac.2016.06.100
[12]	丛大利. 荞麦蜂花粉多糖的分离纯化研究[D]. 长春:东北师范大学, 2011. [CONG Dali. Isolation, purification and structure analysis of polysaccharides from bee pollen of Fagopyrum esculentum Moench[D]. Changchun:Northeast Normal University, 2011.] CONG Dali. Isolation, purification and structure analysis of polysaccharides from bee pollen of Fagopyrum esculentum Moench[D]. Changchun: Northeast Normal University, 2011.
[13]	李娟, 钟平娟, 万仁口, 等. 荞麦蜂花粉多糖的分离纯化及结构鉴定[J]. 食品工业科技,2020,41(12):35−40. [LI Juan, ZHONG Pingjuan, WAN Renkou, et al. Isolation, purification and structure identification of polysaccharides from Fagopyrum esculentum Moench bee pollen[J]. Science and Technology of Food Industry,2020,41(12):35−40.] LI Juan, ZHONG Pingjuan, WAN Renkou, et al. Isolation, purification and structure identification of polysaccharides from Fagopyrum esculentum Moench bee pollen[J]. Science and Technology of Food Industry, 2020, 41（12）: 35−40.
[14]	中华人民共和国卫生部. SN/T 4260-2015出口植物源食品中粗多糖的测定苯酚-硫酸法[S]. 北京:中国标准出版社, 2016. [Ministry of Health. SN/T 4260-2015 Determination of crude polysaccharides in plant source foods for export-Phehol-sulfuric acid colorimetry[S]. Beijing:China Standards Publishing House, 2016.] Ministry of Health. SN/T 4260-2015 Determination of crude polysaccharides in plant source foods for export-Phehol-sulfuric acid colorimetry[S]. Beijing: China Standards Publishing House, 2016.
[15]	温文娟, 刘珊, 黄远丽, 等. 苯酚硫酸法与蒽酮硫酸法测定香菇多糖含量比较[J]. 现代食品,2020(21):177−179. [WEN Wenjuan, LIU Shan, HUANG Yuanli, et al. Comparison of phenol sulfuric and anthrone sulfuric method for determination of lentinan[J]. Modern Food,2020(21):177−179.] WEN Wenjuan, LIU Shan, HUANG Yuanli, et al. Comparison of phenol sulfuric and anthrone sulfuric method for determination of lentinan[J]. Modern Food, 2020（21）: 177−179.
[16]	李书倩, 辛广, 张博, 等. 红蘑多糖的纤维柱层析分离纯化[J]. 食品科技,2012,37(12):170−173. [LI Shuqian, XIN Guang, ZHANG Bo, et al. Separation and purification of Gomphidius rutilus polysaccharides by column chromatography[J]. Food Science and Technology,2012,37(12):170−173.] LI Shuqian, XIN Guang, ZHANG Bo, et al. Separation and purification of Gomphidius rutilus polysaccharides by column chromatography[J]. Food Science and Technology, 2012, 37（12）: 170−173.
[17]	XU Xiaolan, ZHENG Yanzhen. Identification of cattail pollen, pine pollen and bee pollen by Fourier transform infrared spectroscopy and two-dimensional correlation infrared spectroscopy[J]. Journal of Molecular Structure,2018,1167:78−81. doi: 10.1016/j.molstruc.2018.04.076
[18]	成显波. 柱前衍生化高效液相色谱法测定多糖水解液中葡萄糖含量[J]. 轻工科技,2021,37(9):3−4,14. [CHENG Xianbo. Determination of glucose in polysaccharide hydrolysate by pre-column derivatization high performance liquid chromatography[J]. Journal of Food Science and Technology,2021,37(9):3−4,14.] CHENG Xianbo. Determination of glucose in polysaccharide hydrolysate by pre-column derivatization high performance liquid chromatography[J]. Journal of Food Science and Technology, 2021, 37（9）: 3−4,14.
[19]	李珊珊. 六种蜂花粉果胶的结构分析及抑制神经氨酸酶的活性研究[D]. 长春:东北师范大学, 2013. [LI Shanshan. Structure analysis of pectic polysaccharides from six types of bee pollen and their inhibitory activities on neuraminidase[D]. Changchun:Northeast Normal University, 2013.] LI Shanshan. Structure analysis of pectic polysaccharides from six types of bee pollen and their inhibitory activities on neuraminidase[D]. Changchun: Northeast Normal University, 2013.
[20]	CELEP E, CHAREHSAZ M, AKYUZ S, et al. Effect of in vitro gastrointestinal digestion on the bioavailability of phenolic components and the antioxidant potentials of some Turkish fruit wines[J]. Food Research International,2015,78:209−215. doi: 10.1016/j.foodres.2015.10.009
[21]	GUEDES A C, AMARO H M, GIÃO M S, et al. Optimization of ABTS radical cation assay specifically for determination of antioxidant capacity of intracellular extracts of microalgae and cyanobacteria[J]. Food Chemistry,2013,138(1):638−643. doi: 10.1016/j.foodchem.2012.09.106
[22]	林秀. 杏鲍菇多糖提取、分离纯化及抗氧化活性的研究[D]. 福州:福建农林大学, 2014. [LIN Xiu. Study on extraction, purification and antioxidative activity of polysaccharides from Pleurotus eryngii[D]. Fuzhou:Fujian Agriculture and Forestry University, 2014.] LIN Xiu. Study on extraction, purification and antioxidative activity of polysaccharides from Pleurotus eryngii[D]. Fuzhou: Fujian Agriculture and Forestry University, 2014.
[23]	CHEN Ya, XU Miao, WANG Xinyi, et al. Preparation of wood ear medicinal mushroom, Auricularia auricula-judae (Agaricomycetes), melanin and its antioxidant properties:Evaluation in vitro and in vivo[J]. International Journal of Medicinal Mushrooms,2021,23(5):41−53. doi: 10.1615/IntJMedMushrooms.2021038136
[24]	李奉. 荞麦蜂花粉中化学成分研究[D]. 西安:西北大学, 2018. [LI Feng. Chemical constituents from F. esculentum bee pollen[D]. Xi'an:Northwestern University, 2018.] LI Feng. Chemical constituents from F. esculentum bee pollen[D]. Xi'an: Northwestern University, 2018.
[25]	陈路燕, 左绍远. 蜂花粉多糖的提取与分离纯化及影响[J]. 蜜蜂杂志,2017,37(12):4−7. [CHEN Luyan, ZUO Shaoyuan. Extraction and purification of bee pollen polysaccharide and influencing factors[J]. Journal of Bee(Monthly),2017,37(12):4−7.] doi: 10.3969/j.issn.1003-9139.2017.12.004 CHEN Luyan, ZUO Shaoyuan. Extraction and purification of bee pollen polysaccharide and influencing factors[J]. Journal of Bee（Monthly）, 2017, 37（12）: 4−7. doi: 10.3969/j.issn.1003-9139.2017.12.004
[26]	张巧, 陈春喜, 吴天保, 等. 复合酶酶解对大果山楂出汁率及营养品质的影响[J]. 轻工科技, 2021, 37(4):33−35,46. [ZHANG Qiao, CHEN Chunxi, WU Tianbao, et al. Effect of complex enzymatic hydrolysis on juice yield and nutritional quality of Crataegus pinnatifida[J]. Journal of Food Science and Technology 2021, 37(4):33–35,46.] ZHANG Qiao, CHEN Chunxi, WU Tianbao, et al. Effect of complex enzymatic hydrolysis on juice yield and nutritional quality of Crataegus pinnatifida[J]. Journal of Food Science and Technology 2021, 37（4）: 33–35,46.
[27]	陈树俊, 李佳益, 王翠连, 等. 黄梨渣多糖的的提取、分离纯化和结构鉴定[J]. 食品科学,2018,39(20):289−297. [CHEN Shujun, LI Jiayi, WANG Cuilian, et al. Extraction, purification and structural characterization of polysaccharides from pear pomace[J]. Food Science,2018,39(20):289−297.] CHEN Shujun, LI Jiayi, WANG Cuilian, et al. Extraction, purification and structural characterization of polysaccharides from pear pomace[J]. Food Science, 2018, 39（20）: 289−297.
[28]	ANA M A, LAURA T, JOSÉ B, et al Determination of free amino acids in bee pollen by liquid chromatography with fluorescence detection[J]. Food Analytical Methods, 2022, 15(8):2172–2180.
[29]	贾红倩, 刘嵬, 梁立, 等. 红曲霉菌胞外多米糖的分离纯化、结构鉴定及抗氧化活性测定[J]. 食品工业科技,2017,38(12):92−96. [JIA Hongqian, LIU Wei, LIANG Li, et al. Isolation and purification, structure identification and antioxidant activity of Monascus purpureus exopolysaccharide[J]. Science and Technology of Food Industry,2017,38(12):92−96.] JIA Hongqian, LIU Wei, LIANG Li, et al. Isolation and purification, structure identification and antioxidant activity of Monascus purpureus exopolysaccharide[J]. Science and Technology of Food Industry, 2017, 38（12）: 92−96.
[30]	KAC U M, CAPEK P, SACUBJIVÁ V, et al. FT-IR study of plant cell wall model compounds:Pectic polysaccharides and hemicelluloses[J]. Carbohydrate Polymers,2000,43(2):195−203. doi: 10.1016/S0144-8617(00)00151-X
[31]	KERSTIN M J, CAROLIN Б, DIETER T, et al. Simulated digestion of Vitis vinifera seeds powder polyphenolic content and antioxidant properties[J]. Journal of Agricultural and Food Chemistry,2006,54(13):4839−4848. doi: 10.1021/jf052989k
[32]	米佳, 杨雪莲, 禄璐, 等. 枸杞蜂花粉多糖超声波提取工艺优化及抗氧化活性分析[J]. 食品科学技术学报,2020,38(1):97−103. [MI Jia, YANG Xuejia, LU Lu, et al. Ultrasound-assisted extraction optimization and analysis of antioxidant activities of polysaccharides from bee pollen of wolfberry[J]. Journal of Food Science and Technology,2020,38(1):97−103.] doi: 10.3969/j.issn.2095-6002.2020.01.013 MI Jia, YANG Xuejia, LU Lu, et al. Ultrasound-assisted extraction optimization and analysis of antioxidant activities of polysaccharides from bee pollen of wolfberry[J]. Journal of Food Science and Technology, 2020, 38（1）: 97−103. doi: 10.3969/j.issn.2095-6002.2020.01.013