Studies on Isolation, Purification and Gastric Mucosal Protective Activity <i>in Vitro</i> of Laoxianghuang Polysaccharide

YANG Dan; CAI Shu; PENG Chenghai; CHEN Shuxi; ZHOU Aimei

doi:10.13386/j.issn1002-0306.2023010008

Volume 44 Issue 19

Oct. 2023

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Science and Technology of Food Industry > 2023 > 44(19): 440-448. > DOI: 10.13386/j.issn1002-0306.2023010008

YANG Dan, CAI Shu, PENG Chenghai, et al. Studies on Isolation, Purification and Gastric Mucosal Protective Activity in Vitro of Laoxianghuang Polysaccharide[J]. Science and Technology of Food Industry, 2023, 44(19): 440−448. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023010008.

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Studies on Isolation, Purification and Gastric Mucosal Protective Activity in Vitro of Laoxianghuang Polysaccharide

1.
Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China
2.
Guangdong Zhancui Food Co., Ltd., Chaozhou 515634, China
3.
Huanong (Chaozhou) Food Research Institute Co., Ltd., Chaozhou 521021, China

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Received Date: January 03, 2023
Available Online: August 04, 2023

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Abstract

Abstract

In this study, Laoxianghuang polysaccharide was used as the experimental material to examine the protective effects on stomach mucosa. The polysaccharide was first extracted by hot water and then purified by Sevag methods deproteinization, DEAE-52 cellulose anion-exchange chromatography column, and CL-6B agarose gel column. The ethanol-damaged human gastric mucosal epithelial cells (GES-1) model and the GES-1 scratch injury model were used to evaluate gastric mucosal protective activity of the polysaccharide in vitro. Results showed that six fractions of Laoxianghuang polysaccharide were obtained, including PFCP, PFCP-1, PFCP-2, PFCP-3, PFCP-2-1, and PFCP-2-2. After ethanol damaged to GES-1, each fraction of Laoxianghuang polysaccharide improved cellular activity, increased the activity of superoxide dismutase (SOD), catalase (CAT), and inhibited malondialdehyde (MDA) content, exhibiting good antioxidant capacity to reduce mucosal damage. Among them, PFCP-2-1 (50 μg/mL) had the best effect on improving SOD enzyme activity and reducing MDA content, and PFCP-2-1 (5 μg/mL) had the best effect on improving CAT enzyme activity, which was significant compared with the model group (P<0.05) and better than the teprenone-positive drug group. It was demonstrated that Laoxianghuang polysaccharide all fractions accelerated gastric epithelial cell growth, promoted cell migration to the damaged area, and restored gastric mucosal epithelial cell integrity in the GES-1 scratch injury assay. Positive drugs had the most effective scratch repair rate, followed by PFCP-2-1（50 μg/mL）, but there was no significant difference between them (P>0.05). In conclusion, Laoxianghuang polysaccharide PFCP-2-1 may exert gastric mucosal protective activity by enhancing cellular antioxidant capacity as well as promoting the proliferation and migration of GES-1.
- Laoxianghuang,
- polysaccharide,
- human gastric mucosal epithelial cell (GES-1 cell),
- gastric mucosal protection

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References

[1]	刘亚文, 刘红, 吴静. 胃黏膜保护机制与药物研究进展[J]. 临床药物治疗杂志,2018,16(10):8−12. [LIU Yawen, LIU Hong, WU Jing. Progress in gastric mucosa protectant[J]. Clinic Medication Journal,2018,16(10):8−12. LIU Yawen, LIU Hong, WU Jing. Progress in gastric mucosa protectant[J]. Clinic Medication Journal, 2018, 16(10): 8-12.
[2]	YANG Y, YIN B, LV L, et al. Gastroprotective effect of aucubin against ethanol-induced gastric mucosal injury in mice[J]. Life Sciences,2017,189:44−51. doi: 10.1016/j.lfs.2017.09.016
[3]	WU T, LIANG J, AI J, et al. Mulberry ethanol extract and rutin protect alcohol-damaged GES-1 cells by inhibiting the MAPK pathway[J]. Molecules,2022,27(13):4266. doi: 10.3390/molecules27134266
[4]	XU C, DING C, ZHOU N, et al. A polysaccharide from Aloe vera L. var. chinensis (Haw.) Berger prevents damage to human gastric epithelial cells in vitro and to rat gastric mucosa in vivo[J]. Journal of Functional Foods,2016,24:501−512. doi: 10.1016/j.jff.2016.04.035
[5]	WANG R, WU Y, ZHU Y, et al. ANKRD22 is a novel therapeutic target for gastric mucosal injury[J]. Biomedicine & Pharmacotherapy,2022,147:112649.
[6]	WANG X, YIN J, ZHAO M, et al. Gastroprotective activity of polysaccharide from Hericium erinaceus against ethanol-induced gastric mucosal lesion and pylorus ligation-induced gastric ulcer, and its antioxidant activities[J]. Carbohydrate Polymers,2018,186:100−109. doi: 10.1016/j.carbpol.2018.01.004
[7]	薛志远. 红芪多糖的结构表征及抗胃溃疡活性研究[D]. 兰州: 兰州大学, 2022 XUE Zhiyuan. Structural characterization of polysaccharides from radix hedysari andits anti-gastric ulcer activity[D]. Lanzhou: Lanzhou University, 2022.
[8]	丁丽婷, 潘世杰, 胡婕伦, 等. 燕麦多糖对小鼠急性胃黏膜损伤的保护作用[J]. 食品科学,2022,43(9):150−157. [DING Liting, PAN Shijie, HU Jielun, et al. Protective effect of oat polysaccharides against acute gastric mucosal injury in mice[J]. Food Science,2022,43(9):150−157. DING Liting, PAN Shijie, HU Jielun, et al. Protective effect of oat polysaccharides against acute gastric mucosal injury in mice[J]. Food Science, 2022, 43(9): 150-157.
[9]	LIAN Y Z, LIN I, YANG Y, et al. Gastroprotective effect of Lycium barbarum polysaccharides and C-phycocyanin in rats with ethanol-induced gastric ulcer[J]. International Journal of Biological Macromolecules,2020,165:1519−1528. doi: 10.1016/j.ijbiomac.2020.10.037
[10]	阳丹, 陈小爱, 杨玉洁, 等. 基于电子鼻、HS-GC-IMS、HS-SPME-GC-MS技术联用分析不同发酵年份老香黄挥发性成分差异[J]. 现代食品科技,2022,38(11):313−323. [YANG Dan, CHEN Xiaoai, YANG Yujie, et al. The Application of E-nose, HS-GC-IMS, and HS-SPME-GC-MS to differentiate the volatile composition of Lao Xianghuang fermented for different years[J]. Modern Food Science and Technology,2022,38(11):313−323. doi: 10.13982/j.mfst.1673-9078.2022.11.1406 YANG Dan, CHEN Xiaoai, YANG Yujie, et al. The Application of E-nose, HS-GC-IMS, and HS-SPME-GC-MS to differentiate the volatile composition of Lao Xianghuang fermented for different years[J]. Modern Food Science and Technology,2022,38(11):313-323. doi: 10.13982/j.mfst.1673-9078.2022.11.1406
[11]	郭舒臣, 郑玉忠, 郭瑞, 等. 不同年份老香黄定量分析及其化学模式识别研究[J]. 分析测试学报,2021,40(1):10−18. [GUO Shuchen, ZHENG Yuzhong, GUO Rui, et al. Quantitative analysis and chemical pattern recognition of Lao-Xiang-Huang preserved in different years[J]. Journal of Instrumental Analysis,2021,40(1):10−18. GUO Shuchen, ZHENG Yuzhong, GUO Rui, et al. Quantitative analysis and chemical pattern recognition of Lao-Xiang-Huang preserved in different years[J]. Journal of Instrumental Analysis, 2021, 40(1): 10-18.
[12]	林婉玲, 刘亚群, 刘谋泉, 等. 不同陈化年份老香黄品质的综合评价[J]. 食品工业科技,2023,44(2):358−368. [LIN Wanling, LIU Yaqun, LIU Mouquan, et al. Comprehensive evaluation of Laoxinghuang quality in different aging years[J]. Science and Technology of Food Industry,2023,44(2):358−368. doi: 10.13386/j.issn1002-0306.2022040129 LIN Wanling, LIU Yaqun, LIU Mouquan, et al. Comprehensive evaluation of Laoxinghuang quality in different aging years[J]. Science and Technology of Food Industry,2023,44(2):358-368. doi: 10.13386/j.issn1002-0306.2022040129
[13]	陈小爱, 蔡惠钿, 刘静宜, 等. 基于电子鼻、GC-MS和GC-IMS技术分析老香黄发酵期间的挥发性成分变化[J]. 食品工业科技,2021,42(12):70−80. [CHEN Xiaoai, CAI Huitian, LIU Jingyi, et al. Analysis of volatile components in Laoxianghuang during fermentation by electronic nose, GC-MS and GC-IMS[J]. Science and Technology of Food Industry,2021,42(12):70−80. CHEN Xiaoai, CAI Huitian, LIU Jingyi, et al. Analysis of Volatile Components in Laoxianghuang During Fermentation by Electronic Nose, GC-MS and GC-IMS[J]. Science and Technology of Food Industry, 2021, 42 (12): 70-80.
[14]	赖宣, 杨启财, 张振霞. 潮汕老香黄亚硝酸盐含量的调查分析[J]. 轻工科技,2016,32(5):4−5. [LAI Xuan, YANG Shoucai, ZHANG Zhengxia. Investigation and analysis of nitrite content of Lao Xianghuang in Chaoshan[J]. Light Industry Science and Technology,2016,32(5):4−5. LAI Xuan, YANG Shoucai, ZHANG Zhengxia. Investigation and analysis of nitrite content of Lao Xianghuang in Chaoshan[J]. Light Industry Science and Technology, 2016, 32(5): 4-5.
[15]	戈子龙, 张泽金, 周爱梅, 等. 基于高通量测序与培养方法分析新鲜佛手与老香黄中的细菌多样性[J]. 食品与发酵工业,2020,46(3):250−256. [GE Zilong, ZHANG Zejin, ZHOU Aimei, et al. Analysis of bacteria diversity in fresh fingered citron and Laoxianghuang based on high throughput sequencing and culture methodology[J]. Food and Fermentation Industries,2020,46(3):250−256. GE Zilong, ZHANG Zejin, ZHOU Aimei, et al. Analysis of bacteria diversity in fresh fingered citron and Laoxianghuang based on high throughput sequencing and culture methodology[J]. Food and Fermentation Industries, 2020, 46(3): 250-256.
[16]	王淑惠, 杨玉洁, 周爱梅, 等. 两种方法提取佛手渣多糖及其对巨噬细胞RAW264.7免疫调节活性的研究[J]. 食品工业科技,2020,41(15):179−187. [WANG Shuhui, YANG Yujie, ZHOU Aimei, et al. Study on the polysaccharide extracted from Bergamot (Citrus medica L. var. sarcodactylis) residue by two methods and its immunomodulatory function in RAW264.7 cells[J]. Science and Technology of Food Industry,2020,41(15):179−187. WANG Shuhui, YANG Yujie, ZHOU Aimei, et al. Study on the polysaccharide extracted from Bergamot ( Citrus medica L. var. sarcodactylis) residue by two methods and its immunomodulatory function in RAW264.7 cells[J]. Science and Technology of Food Industry, 2020, 41(15): 179-187.
[17]	YUAN L, QIU Z, YANG Y, et al. Preparation, structural characterization and antioxidant activity of water-soluble polysaccharides and purified fractions from blackened jujube by an activity-oriented approach[J]. Food Chemistry,2022,385:132637. doi: 10.1016/j.foodchem.2022.132637
[18]	袁心田, 陈华国, 赵超, 等. 基于膜技术的桑葚多糖分级分离及生物活性组分筛选[J]. 食品工业科技,2022,43(24):672−80. [YUAN Xintian, CHEN Huaguo, ZHAO Chao, et al. Separation of Mori fructus polysaccharides and screening of bioactive fractions based on membrane technology[J]. Science and Technology of Food Industry,2022,43(24):672−80. YUAN Xintian, CHEN Huaguo, ZHAO Chao, et al. Separation of Mori fructus polysaccharides and screening of bioactive fractions based on membrane technology[J]. Science and Technology of Food Industry, 2022, 43(24): 672-80.
[19]	方青青. 油茶桑寄生多糖的提取纯化、结构分析及其生物活性研究[D]. 湘潭: 湘潭大学, 2019 FANG Qingqing. The extraction, purification, structural analysis and bioactivity research of polysaccharide from Mistletoe of Camellia Oleifera[D]. Xiangtan: Xiangtan University, 2019.
[20]	XIE M, CHEN H, NIE S, et al. Gastroprotective effect of gamma-aminobutyric acid against ethanol-induced gastric mucosal injury[J]. Chemico-Biological Interactions,2017,272:125−134. doi: 10.1016/j.cbi.2017.04.022
[21]	LIU D, WANG S, BAO Y, et al. Extraction, purification and structural characterization of polysaccharides from Apocynum venetum L. roots with anti-inflammatory activity[J]. Process Biochemistry,2022,121:100−112. doi: 10.1016/j.procbio.2022.06.035
[22]	ZHANG R, ZHANG X, TANG Y, et al. Composition, isolation, purification and biological activities of Sargassum fusiforme polysaccharides: A review[J]. Carbohydrate Polymers,2020,228:115381. doi: 10.1016/j.carbpol.2019.115381
[23]	LIN B, HUANG G. Extraction, isolation, purification, derivatization, bioactivity, structure-activity relationship, and application of polysaccharides from White jellyfungus[J]. Biotechnology and Bioengineering,2022,119(6):1359−1379. doi: 10.1002/bit.28064
[24]	张如佳. 锦灯笼果实多糖的分离纯化及结构分析[D]. 长春: 东北师范大学, 2020 ZHANG Rujia. Isolation, purification and structural analysis of polysaccharides from the fruits of Physalis alkekengi L. [D]. Changchun: Northeast Normal University, 2020.
[25]	ZHANG C, GAO F, GAN S, et al. Chemical characterization and gastroprotective effect of an isolated polysaccharide fraction from Bletilla striata against ethanol-induced acute gastric ulcer[J]. Food and Chemical Toxicology,2019,131:110539. doi: 10.1016/j.fct.2019.05.047
[26]	CHARI S, TEYSSEN S, SINGER M V. Alcohol and gastric acid secretion in humans[J]. Gut,1993,34(6):843−847. doi: 10.1136/gut.34.6.843
[27]	何瑞坤, 黄焕迪, 戴庆玲, 等. 高良姜、红豆蔻及大高良姜的挥发油对乙醇致GES-1细胞损伤的保护作用及机制研究[J]. 中华中医药学刊,2022,40(6):81−85. [HE Ruikun, HUANG Huandi, DAI Qingling, et al. The protective effect and mechanism of volatile oil from Alpinia officinarum Hance, seeds of Alpinia galanga Willd and Alpinia galanga Willd on GES-1 cell damage induced by ethanol[J]. Chinese Archives of Traditional Chinese Medicine,2022,40(6):81−85. HE Ruikun, HUANG Huandi, DAI Qingling, et al. The protective effect and mechanism of volatile oil from Alpinia officinarum Hance, seeds of Alpinia galanga Willd and Alpinia galanga Willd on GES-1 cell damage induced by ethanol[J]. Chinese Archives of Traditional Chinese Medicine, 2022, 40(6): 81-85.
[28]	廖兵武. 猴头菇多糖及其磷酸化衍生物对GES-1细胞损伤的保护作用研究[D]. 广州: 华南理工大学, 2021 LIAO Binwu. A study on protective effects of polysaccharide from Hericium erinaceus and its phosphorylated derivatives against injured GES-1 cells[D]. Guangzhou: South China University of Technology, 2021.
[29]	LIU H, ZHUANG S, LIANG C, et al. Effects of a polysaccharide extract from Amomum villosum Lour. on gastric mucosal injury and its potential underlying mechanism[J]. Carbohydrate Polymers,2022,294:119822. doi: 10.1016/j.carbpol.2022.119822
[30]	KE Y, ZHAN L, LU T, et al. Polysaccharides of Dendrobium officinale Kimura & Migo leaves protect against ethanol-induced gastric mucosal injury via the AMPK/mTOR signaling pathway in vitro and vivo[J]. Frontiers in Pharmacology,2020,11:526349.
[31]	WANG X Y, YIN J Y, HU J L, et al. Gastroprotective polysaccharide from natural sources: Review on structure, mechanism, and structure-activity relationship[J]. Food Frontiers,2022,3(4):560−591. doi: 10.1002/fft2.172
[32]	WANG B, ZHANG H, CHEN L, et al. Extraction, purification, and determination of the gastroprotective activity of glucomannan from Bletilla striata[J]. Carbohydrate Polymers,2020,246:116620. doi: 10.1016/j.carbpol.2020.116620
[33]	剌晓琴, 刘一志, 张立超, 等. 谷糠多酚对酒精性胃黏膜损伤的保护作用[J]. 食品科学,2022,43(13):64−71. [LA Xiaoqin, LIU Yizhi, ZHANG Lichao, et al. Protective effect of Foxtail Millet bran-derived polyphenols on Alcohol-Induced Gastric Mucosal Injury[J]. Food Science,2022,43(13):64−71. LA Xiaoqin, LIU Yizhi, ZHANG Lichao, et al. Protective effect of Foxtail Millet bran-derived polyphenols on Alcohol-Induced Gastric Mucosal Injury[J]. Food Science, 2022, 43 (13): 64-71.
[34]	张慧. 白及多糖对乙醇型胃黏膜损伤保护作用的研究[D]. 西安: 陕西师范大学, 2018 ZHANG Hui. Study on the protective effect of Bletilla striata polysaccharide on ethanol-based gastric mucosal damage[D]. Xi'an: Shaanxi Normal University, 2018.
[35]	DAS K, ROYCHOUDHURY A. Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants[J]. Frontiers in Environmental Science, 2014, 2.
[36]	HOU C, LIU L, REN J, et al. Structural characterization of two Hericium erinaceus polysaccharides and their protective effects on the alcohol-induced gastric mucosal injury[J]. Food Chemistry,2022,375:131896. doi: 10.1016/j.foodchem.2021.131896
[37]	PAN J S, HE S Z, XU H Z, et al. Oxidative stress disturbs energy metabolism of mitochondria in ethanol-induced gastric mucosa injury[J]. World Journal of Gastroenterology,2008(38):5857−5867.
[38]	LAINE L, TAKEUCHI K, TARNAWSKI A. Gastric mucosal defense and cytoprotection: Bench to bedside[J]. Gastroenterology,2008,135(1):41−60. doi: 10.1053/j.gastro.2008.05.030
[39]	陈敏敏. 胃黏膜防御—修复机制老化的初步研究[D]. 上海: 复旦大学, 2013 CHEN Mingming. Gastric mucosal defense-repair mechanisms with aging: A preliminary study[D].Shanghai: Fudan University, 2013.
[40]	林娇芬, 黄家福, 林志超, 等. 杏鲍菇β-葡聚糖促人胃粘膜上皮细胞增殖和迁移效果研究[J]. 热带作物学报,2013,34(11):2301−2306. [LIN Jiaofen, HUANG Jiafu, LIN Zhichao, et al. The Effect of β-glucan from Pleurotus Eryngii on promoting the proliteration and migration of gastric mucosa epithelial cell[J]. Chinese Journal of Tropical Crops,2013,34(11):2301−2306. doi: 10.3969/j.issn.1000-2561.2013.11.039 LIN Jiaofen, HUANG Jiafu, LIN Zhichao, et al. The Effect of β-glucan from Pleurotus Eryngii on promoting the proliteration and migration of gastric mucosa epithelial cell[J]. Chinese Journal of Tropical Crops, 2013, 34(11): 2301-2306. doi: 10.3969/j.issn.1000-2561.2013.11.039

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