Anti-aging Effect and Mechanism of Ethanol Extract of <i>Clinacanthus nutans</i> on <i>Caenorhabditis elegans</i>

ZHUANG Yanping; YANG Fan; XIAO Man; TIAN Xiaoyu; CHEN Yi; LONG Ziyu; CHEN Bocen; NI Yali; GONG Aimin; XIE Yiqiang

doi:10.13386/j.issn1002-0306.2022090024

Volume 44 Issue 11

May 2023

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2023 > 44(11): 411-417. > DOI: 10.13386/j.issn1002-0306.2022090024

ZHUANG Yanping, YANG Fan, XIAO Man, et al. Anti-aging Effect and Mechanism of Ethanol Extract of Clinacanthus nutans on Caenorhabditis elegans[J]. Science and Technology of Food Industry, 2023, 44(11): 411−417. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090024.

Citation:

PDF (1597 KB)

Anti-aging Effect and Mechanism of Ethanol Extract of Clinacanthus nutans on Caenorhabditis elegans

1.
College of Traditional Chinese Medicine, Hainan Medical College, Haikou 571199, China
2.
Department of Pharmacy, the Second People's Hospital of Hainan Province, Haikou 572299, China

More Information

Received Date: September 04, 2022
Available Online: March 29, 2023

Graphical Abstract

Abstract

Abstract

Objective: To assess the anti-aging effect and mechanism underlying the effects of ethanol extract from Clinacanthus nutans (CNEE) using Caenorhabditis elegans as the model organism. Methods: The anti-aging effects of ethanol extract from Clinacanthus nutans on nematodes were detected by experiments to assay longevity, oviposition, and exercise. The antioxidant capacity of the ethanolic extracts from Clinacanthus nutans and their mechanisms of action in delaying aging were investigated by assessing the acute oxidative stress, determining the antioxidant enzyme activity, and determining the reactive oxygen species (ROS) levels along with real-time fluorescence quantitative PCR experiments. Results: The ethanol extract from Clinacanthus nutans at 100 μg/mL could prolong the life span of N2 wild type nematode (P<0.001), promote the ability to exercise (P<0.001), and exerted no toxic effects on oviposition (P>0.05). The extract also increased nematode longevity (P<0.05) and catalase activity (P<0.01) under acute oxidative stress conditions, and effectively reduced the accumulation of ROS in vivo (P<0.05) along with upregulating the levels of daf-16 (P<0.01) and downstream ctl-1 (P<0.001) gene expressions. However, in daf-16 mutant nematodes, effects on life extension under normal conditions or acute oxidative stress were not observed, nor was there an increase in catalase activity, a decrease in ROS levels, or an increase in daf-16 and ctl-1 levels (P>0.05). Conclusion: The ethanol extract from Clinacanthus nutans enhanced the ability of nematodes to resist aging and the underlying mechanism of action may be related to the regulation of oxidative stress by daf-16.
- Clinacanthus nutans ethanol extract,
- Caenorhabditis elegans,
- anti-aging,
- antioxidant,
- daf-16

FullText(HTML)

References (33)

References

[1]	刘赫男. 富氢水的制备及丁酸梭菌延长秀丽线虫寿命的机制研究[D]. 合肥: 安徽医科大学, 2020 LIU H N. Preparation of hydrogen-rich water and the mechanism of Clostridium butyricum to prolong the life span of Caenorhabditis elegans[D]. Hefei: Anhui Medical University, 2020.
[2]	黄小兵. 抗衰老化合物筛选及阿司匹林抗衰老活性评价[D]. 昆明: 中科院昆明植物研究所, 2016 HUANG X B. Screening of anti-aging compounds and evaluation of anti-aging activity of aspirin[D]. Kunming: Kunming Institute of Botany, Chinese Academy of Sciences, 2016.
[3]	GUO X Y, LI Q Q, SHI J, et al. Perfluorooctane sulfonate exposure causes gonadal developmental toxicity in Caenorhabditis elegans through ROS-induced DNA damage[J]. Chemosphere,2016,155:115−126. doi: 10.1016/j.chemosphere.2016.04.046
[4]	SHAY J W. Role of telomeres and telomerase in aging and cancer[J]. Cancer Discovery,2016,6(6):584−593. doi: 10.1158/2159-8290.CD-16-0062
[5]	段佳辉. 忧遁草叶提取物解酒、保肝及抗氧化功能的研究[D]. 无锡: 江南大学, 2019 DUAN J H. Study on the anti-drinking, hepatoprotective and antioxidant functions of the leaf extracts of Clinacanthus nutans[D]. Wuxi: Jiangnan University, 2019.
[6]	王瑶, 钟希文, 张文霞. 鳄嘴花的化学成分及药理作用研究进展[J]. 中国药房,2016,27(1):108−111. [WANG Y, ZHONG X W, ZHANG W X. Advances in the chemical composition and pharmacological effects of Clinacanthus nutans[J]. Chinese Pharmacy,2016,27(1):108−111.
[7]	魏瑞. 忧遁草中三萜类物质的提取纯化及功能评价[D]. 天津: 天津科技大学, 2020 WEI R. Extraction, purification and functional evaluation of triterpenoids from Clinacanthus nutans[D]. Tianjin: Tianjin University of Science and Technology, 2020.
[8]	段佳辉, 徐德平, 钱志娟, 等. 忧遁草抗氧化功效与成分的研究[J]. 食品科技,2019,44(3):205−210. [DUAN J H, XU D P, QIAN Z J, et al. Study on the antioxidant efficacy and composition of Clinacanthus nutans[J]. Food Science and Technology,2019,44(3):205−210.
[9]	黄壮, 李静, 杜鸿志, 等. 三七醇提取物对秀丽隐杆线虫的降脂作用[J]. 世界科学技术-中医药现代化,2020,22(5):1648−1653. [HUANG Z, LI J, DU H Z, et al. Lipid lowering effect of Panax notoginseng alcohol extract on Caenorhabditis elegans[J]. World Science and Technology-Modernization of Traditional Chinese Medicine,2020,22(5):1648−1653.
[10]	刘静. 金钗石斛总生物碱抗秀丽隐杆线虫衰老作用及其机制研究[D]. 遵义: 遵义医科大学, 2019 LIU J. Study on the anti aging effect and mechanism of total alkaloids of Dendrobium nobile on Caenorhabditis elegans[D]. Zunyi: Zunyi Medical University, 2019.
[11]	HERNDON L A, WOLKOW C A, DRISCOLL M, et al. Effects of ageing on the basic biology and anatomy of C. elegans[M]. Switzerland in Ageing Lessons from C. elegans, 2017: 9−39.
[12]	TISSENBAUM H A. Using C. elegans for aging research[J]. Invertebrate RE Production & Development,2015,59(1):59−63.
[13]	普元柱. 基于蛋白质组学研究秀丽线虫生殖系统调控衰老的机制[D]. 北京: 中国科学院大学, 2017 PU Y Z. Proteomics based study on the mechanism of the reproductive system of Caenorhabditis elegans regulating aging [D]. Beijing: University of Chinese Academy of Sciences, 2017.
[14]	张宗敏. 金钗石斛多糖对秀丽隐杆线虫衰老的影响[D]. 遵义: 遵义医学院, 2018 ZHANG Z M. Effects of Dendrobium nobile polysaccharides on the senescence of C. elegans[D]. Zunyi: Zunyi Medical College, 2018.
[15]	刘春红, 汤燚聪, 高瑜培, 等. 鹿茸乙醇提取物对秀丽隐杆线虫抗衰老的作用[J]. 食品工业科技,2021,42(7):354−359. [LIU C H, TANG Y C, GAO Y P, et al. Anti aging effect of ethanol extract of cartialgenous on C. elegans[J]. Science and Technology of Food Industry,2021,42(7):354−359.
[16]	LOPEZ-GARCIA G, CILLA A, BARBERA R, et al. Effect of plant sterol and galactooligosaccharides enriched beverages on oxidative stress and longevity in Caenorhabditis elegans[J]. Journal of Functional Foods,2020,65:1−8.
[17]	MARIA D N H, JOSE M R M, ADELAIDA E M, et al. Strawberry (Fragaria×ananassa cv. Romina) methanolic extract attenuates Alzheimer's beta amyloid production and oxidative stress by SKN-1/NRF and DAF-16/FOXO mediated mechanisms in C. elegans[J]. Food Chemistry,2022,372:1−12.
[18]	于群. 忧遁草活性成分的提取及其功能评价[D]. 海口: 海南大学, 2017 YU Q. Extraction and functional evaluation of the active ingredients of Clinacanthus nutans[D]. Haikou: Hainan University, 2017.
[19]	杨帆, 肖曼, 陈柏岑, 等. 益智仁提取物延缓秀丽隐杆线虫衰老的研究[J]. 中华中医药学刊,2022,40(2):43−46, 265. [YANG F, XIAO M, CHEN B C, et al. Study on the effect of Alpinia oxyphylla extract on delaying the senescence of C. elegans[J]. Chinese Journal of Traditional Chinese Medicine,2022,40(2):43−46, 265.
[20]	金司仪. 三七粉延缓秀丽隐杆线虫衰老的作用机制及其稳定性研究[D]. 武汉: 湖北中医药大学, 2019 JIN S Y. Study on the mechanism and stability of Panax notoginseng powder in delaying the senescence of C. elegans[D]. Wuhan: Hubei University of Traditional Chinese Medicine, 2019.
[21]	黄少杰, 陈宏著, 钟淳菲, 等. 铁皮石斛叶多糖对秀丽隐杆线虫体内抗衰老作用[J]. 食品科学,2022,43(21):203−208. [HUANG S J, CHEN H, ZHONG C F, et al. Anti aging effect of polysaccharides from Dendrobium nobile on C. elegans in vivo[J]. Food Science,2022,43(21):203−208. doi: 10.7506/spkx1002-6630-20211202-031
[22]	付敏, 张旭光, 杨柳, 等. 虾青素调控ROS介导的自噬延长秀丽隐杆线虫寿命作用[J]. 食品工业科技,2022,43(4):373−378. [FU M, ZHANG X G, YANG L, et al. Astaxanthin regulates ROS mediated autophagy to prolong the life span of C. elegans[J]. Science and Technology of Food Industry,2022,43(4):373−378.
[23]	李煜, 敬海明, 李国君. 秀丽隐杆线虫神经毒理学研究进展[J]. 中国公共卫生,2013,29(4):622−624. [LI Y, JING H M, LI G J. Research progress in neurotoxicology of C. elegans[J]. China Public Health,2013,29(4):622−624. doi: 10.11847/zgggws2013-29-04-58
[24]	周栋. 内分泌干扰物双酚A对秀丽隐杆线虫的生态毒性效应及其作用机制研究[D]. 上海: 华东理工大学, 2016 ZHOU D. Study on the ecotoxicity and mechanism of endocrine disruptor bisphenol A on C. elegans[D]. Shanghai: East China University of Technology, 2016.
[25]	王力, 肖嵋方, 陈弘培, 等. 牡蛎多肽组分OE-I抗氧化活性及其对秀丽隐杆线虫抗衰老作用[J]. 食品科学,2022,43(3):152−160. [WANG L, XIAO M F, CHEN H P, et al. Antioxidant activity of oyster polypeptide OE-I and its anti-aging effect on C. elegans[J]. Food Science,2022,43(3):152−160.
[26]	王锐凡, 雷欣安, 成高丽, 等. 鳄嘴花总黄酮对高糖诱导血管内皮细胞损伤的保护作用及机制研究[J]. 中国循证心血管医学杂志,2019,11(9):1077−1080. [WANG R F, LEI X A, CHENG G L, et al. Study on the protective effect and mechanism of total flavones of Clinacanthus nutans on vascular endothelial cell injury induced by high glucose[J]. Chinese Journal of Evidence Based Cardiovascular Medicine,2019,11(9):1077−1080.
[27]	TYAGI N, GAMBHIR K, KUMAR S, et al. Interplay of reactive oxygen species (ROS) and tissue engineering: A review on clinical aspects of ROS-responsive biomaterials[J]. Journal of Materials Science,2021,56:16790−16823. doi: 10.1007/s10853-021-06338-7
[28]	LIU Y L, ZHOU Z Y, YIN L F, et al. Tangeretin promotes lifespan associated with insulin/ insulin-like growth factor-1 signaling pathway and heat resistance in Caenorhabditis elegans[J]. BioFactors (Oxford, England),2021,48(2):442−453.
[29]	LIN C, XIAO J, XI Y, et al. Rosmarinic acid improved antioxidant properties and healthspan via the IIS and MAPK pathways in Caenorhabditis elegans[J]. BioFactors (Oxford, England),2019,45(5):774−787. doi: 10.1002/biof.1536
[30]	宋兵兵. 蓝莓和苹果皮提取物联合抗衰老活性及作用机制研究[D]. 广州: 华南理工大学, 2020 SONG B B. Study on the combined anti-aging activity and mechanism of blueberry and apple peel extracts[D]. Guangzhou: South China University of Technology, 2020.
[31]	蔡洁. 米糠活性肽通过daf-16和skn-1调节线虫寿命与应激能力的研究[D]. 长沙: 中南林业科技大学, 2021 CAI J. Studies on the regulation of rice bran active peptides on C. elegans longevity and stress capacity through daf-16 and skn-1[D]. Changsha: Central South Forestry University, 2021.
[32]	WAN Q L, FU X D, DAI W Y, et al. Uric acid induces stress resistance and extends the life span through activating the stress response factor DAF-16/FOXO and SKN-1/NRF2[J]. Aging,2020,12(3):2840−2856. doi: 10.18632/aging.102781
[33]	米象男. 3,4-二羟基苯甲酸甲酯延长秀丽线虫寿命的机制研究[D]. 广州: 暨南大学, 2018 MI X N. Study on the mechanism of methyl 3,4-dihydroxybenzoate prolonging the life span of C. elegans[D]. Guangzhou: Jinan University, 2018.

[1]	YIN Hongmei, CHEN Haoran, YIN Ziran, TANG Zhenyi, ZENG Jinxiu, SHI Xiaodan, ZHANG Lu, TU Zongcai. Comparison of Volatile Flavor Compounds and Qualities between Traditional Fermented and Inoculated Fermented of Mei yu[J]. Science and Technology of Food Industry, 2024, 45(15): 56-65. DOI: 10.13386/j.issn1002-0306.2023070267
[2]	ZHANG Shiyao, XIAO Yue, WANG Xinyu, CHU Chuanqi, WANG Tao, HU Xiaosong, YI Junjie. Quality Characteristics of Fermented Chili Peppers Using Different Inoculated and Natural Fermentation Approaches[J]. Science and Technology of Food Industry, 2024, 45(12): 129-139. DOI: 10.13386/j.issn1002-0306.2023070254
[3]	ZHU Jiamin, MO Ziyao, LI Mengtong, XIN Yueqi, ZHU Ying'ao, WANG Hui, CHEN Qian. Study on Different Inoculation Levels of Lactobacillus sakei on the Improvement of Quality Characteristics of Low-sodium Dry Sausages[J]. Science and Technology of Food Industry, 2023, 44(7): 133-142. DOI: 10.13386/j.issn1002-0306.2022060041
[4]	TANG Kaiwei, HUANG Xiaoying, YI Yuwen, ZHU Chenglin, DENG Jing, YE Haixiao, TANG Junni. Effect of Fermentation with Single and Co-culture of Lactobacillus bulgaricus and Streptococcus thermophilus on the Quality of Yogurt[J]. Science and Technology of Food Industry, 2022, 43(23): 127-132. DOI: 10.13386/j.issn1002-0306.2022020250
[5]	LIU Chang, ZUO Changzhou, PENG Jing, CHEN Jikun, TU Kang, PAN Leiqing. Response Surface Optimization of the Fermentation Process of Tomato Juice by Lactobacillus plantarum and Its Quality Evaluation[J]. Science and Technology of Food Industry, 2022, 43(10): 246-253. DOI: 10.13386/j.issn1002-0306.2021080285
[6]	ZHOU Yingjun, XIE Chunliang, CHEN Baizhong, GONG Wenbing, ZHU Zuohua, XU Chao, YANG Qi, PENG Yuande. Effect of Different Yeast and Lactobacillus plantarum Combined Fermentation on the Quality of Xinhui Citrus Ferment[J]. Science and Technology of Food Industry, 2022, 43(6): 118-125. DOI: 10.13386/j.issn1002-0306.2021060189
[7]	ZHANG Lihua, WANG Xia, ZHA Mengmeng, TANG Peixin, SHI Xun, ZONG Wei, ZHAO Guangyuan. Effects of Lactobacillus plantarum Microcapsules Fermentation on the Quality of Carrot Chips[J]. Science and Technology of Food Industry, 2022, 43(2): 135-141. DOI: 10.13386/j.issn1002-0306.2021040234
[8]	ZHANG Li-hua, WANG Xia, ZHAO Guang-yuan, ZONG Wei. Effects of Adding Jujube Juice on Quality of Carrot Fermented by Lactobacillus plantarum[J]. Science and Technology of Food Industry, 2020, 41(15): 99-105. DOI: 10.13386/j.issn1002-0306.2020.15.016
[9]	LIU Ying-ping, CUI Li, NIU Li-ying, LI Da-jing, LIU Chun-quan, XIAO Li-xia. Study on the microwave sterilization of Lactobacillus plantarum and application of microwave on pickled lettuce[J]. Science and Technology of Food Industry, 2016, (24): 245-248. DOI: 10.13386/j.issn1002-0306.2016.24.038
[10]	JIN Le-tian, WU Shi-rong, LIU Tong-jie, HE Guo-qing. Effect of Lactobacillus plantarum inoculated fermentation on the quality of kimchi[J]. Science and Technology of Food Industry, 2014, (23): 195-198. DOI: 10.13386/j.issn1002-0306.2014.23.032

Supplements (0)

Cited By

Cited by

Periodical cited type(11)

1.	吕帅，刘红微，刘帅，董毓卿. 黄芪饮料的配方优化. 食品工业. 2025(03): 4-7 .
2.	樊月月，罗浩，李富豪，徐梦珠，郝娟，谢心. 参芪补胰方通过调节TLR4/MyD88/NF-κB信号通路治疗2型糖尿病大鼠的作用机制. 中华中医药学刊. 2023(06): 237-242+279-281 .
3.	张国栋，张兰. 从脾肾探讨糖尿病足的病机. 实用中医内科杂志. 2023(11): 32-34 .
4.	周敏，吴黎，谭崇赋，徐旭英. 回阳生肌膏抗小鼠骨髓内皮祖细胞功能损伤的研究. 北京中医药大学学报. 2022(01): 41-52 .
5.	冯一帆，吴励萍，张祎盈，钱大玮，段金廒，宋孝雄，吕高虹，朱悦，许惠琴. 杞丹方对糖尿病小鼠降血糖作用及机制研究. 南京中医药大学学报. 2022(06): 504-510 .
6.	陈紫军，霍晓乾，任越，舒展，张燕玲. 复方杜仲健骨颗粒治疗骨关节炎的抗炎潜在药效物质及作用机制研究. 中国中药杂志. 2022(15): 4156-4163 .
7.	姜莉，王成，邢颖，徐怀德. 不同提取方法对黄芪提取液活性成分及抗氧化性的影响. 食品研究与开发. 2022(16): 119-126 .
8.	陈东方，高慧艳，王燕，徐冰，王海玉，张聪恪. 黄芪葛根胶囊降血糖作用的人体试食效果观察. 中国卫生检验杂志. 2022(20): 2442-2445 .
9.	王圣翔，强煜云，布培培. 内毒素致病机理及防治研究进展. 畜牧产业. 2021(11): 73-75 .
10.	孙亚娟. 止嗽散加减治疗慢性咳嗽的疗效及对血清指标的影响. 中国民间疗法. 2021(21): 61-63 .
11.	冉建新，许蕊蕊，王晓杰. 基于小鼠模型筛选黄芪当归治疗2型糖尿病的最佳配比及最优半仿生提取工艺. 中国医药导刊. 2021(11): 828-834 .