TANG Kaiwei, HUANG Xiaoying, YI Yuwen, et al. Effect of Fermentation with Single and Co-culture of Lactobacillus bulgaricus and Streptococcus thermophilus on the Quality of Yogurt[J]. Science and Technology of Food Industry, 2022, 43(23): 127−132. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020250.
Citation: TANG Kaiwei, HUANG Xiaoying, YI Yuwen, et al. Effect of Fermentation with Single and Co-culture of Lactobacillus bulgaricus and Streptococcus thermophilus on the Quality of Yogurt[J]. Science and Technology of Food Industry, 2022, 43(23): 127−132. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020250.

Effect of Fermentation with Single and Co-culture of Lactobacillus bulgaricus and Streptococcus thermophilus on the Quality of Yogurt

More Information
  • Received Date: February 27, 2022
  • Available Online: October 05, 2022
  • In order to investigate the effects of fermentation with single and co-culture of Lactobacillus bulgaricus and Streptococcus thermophilus on the quality of yogurt, the fermentation characteristics, sensory features, rheology and texture of yogurt produced by the above two fermentation approaches were tested. The results showed that the curdling time of co-culture fermented yogurt was 8 h, which was shorter than that of single-strain fermented yogurt, and reached the end point of fermentation at 70 °T earlier. Sensory evaluation evaluated that the scores of co-culture fermentation yogurt were higher than that of single culture yogurt. Moreover, the ratio of Lactobacillus bulgaricus and Streptococcus thermophilus was 3:7 with the highest score. The measurement results of rheological properties showed that the apparent viscosity of co-culture fermented yogurt was 0.4~0.5 Pa·s lower than that of single-bacteria fermented yogurt of 1.6~1.9 Pa·s. As for texture analysis, the hardness, viscosity and absolute value of cohesiveness of co-culture fermented yogurt were significantly lower than that of single culture yogurt (P<0.05). The data of electronic nose showed that the odor of co-culture fermented yogurt was stronger than that of single bacteria fermented yogurt. In addition, electronic tongue test showed that the co-culture fermented yogurt exhibited more balanced in terms of different tastes. Electronic nose and electronic tongue could effectively distinguish the difference between single and co-culture fermented yogurt through principal component analysis. Based on the above results, the taste and flavor of co-culture fermented yogurt are better than that of single bacteria fermented ones.
  • [1]
    LI Siqi, YE Aiqi, HARJINDER S. Effects of seasonal variations on the quality of set yogurt, stirred yogurt, and Greek-style yogurt[J]. J Dairy Sci,2021,104(2):1424−1432. doi: 10.3168/jds.2020-19071
    [2]
    CHAMINDA S R, NENAD N, SAID A. Non-bovine milk products as emerging probiotic carriers: Recent developments and innovations[J]. Current Opinion in Food Science,2018,22:109−114. doi: 10.1016/j.cofs.2018.02.010
    [3]
    李婷, 田佳乐, 刘洋, 等. 保加利亚乳杆菌的筛选及其发酵乳中的风味物质[J]. 中国食品学报,2021,21(3):315−323. [LI Ting, TIAN Jiale, LIU Yang, et al. Screening of Lactobacillus delbrueckii subsp. bulgaricus and its flavor substances in fermented milk[J]. Journal of Chinese Institute of Food Science and Technology,2021,21(3):315−323. doi: 10.16429/j.1009-7848.2021.03.036
    [4]
    丹彤, 乔少婷, 田佳乐. 具有良好风味嗜热链球菌的筛选及其产香特性分析[J]. 食品工业科技,2021,42(16):99−105. [DAN Tong, QIAO Shaoting, TIAN Jiale. Screening of Streptococcus thermophilus with good flavor and analysis of its aroma production characteristics[J]. Science and Technology of Food Industry,2021,42(16):99−105. doi: 10.13386/j.issn1002-0306.2020120219
    [5]
    ERI Y, REIKO W, EMI T, et al. Effect of fumaric acid on the growth of Lactobacillus delbrueckii ssp. bulgaricus during yogurt fermentation[J]. Journal of Dairy Science,2021,104(9):9617−9626. doi: 10.3168/jds.2021-20173
    [6]
    DAN T, WANG D, WU S M, et al. Profiles of volatile flavor compounds in milk fermented with different proportional combinations of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus[J]. Molecules,2017,22(10):1−14.
    [7]
    YILMAZ C, GOKMEN V. Formation of tyramine in yoghurt during fermentation-Interaction between yoghurt starter bacteria and Lactobacillus plantarum[J]. Food Research International, 2017, 97: 288−295.
    [8]
    MASATO O, YOSHIAKI M, TOSHIHIDE N. Sensory properties and taste compounds of fermented milk produced by Lactococcus lactis and Streptococcus thermophilus[J]. Food Science and Technology Research,2008,14(2):183−189. doi: 10.3136/fstr.14.183
    [9]
    蒋鸿波. 耐酸保加利亚乳杆菌在酸奶中的应用[J]. 食品工业,2018,39(4):166−168. [JIANG Hongbo. Applicaton of acid tolerant Lactobacillus bulgaricus in yoghourt fermentation[J]. The Food Industry,2018,39(4):166−168.
    [10]
    苏敦, 任发政, 刘松玲, 等. 菌株发酵特性研究及复合酸奶发酵剂的筛选[J]. 中国奶牛,2017(9):50−57. [SU Dun, REN Fazheng, LIU Songling, et al. Study of bacterial strains fermentation characteristics and development of yoghurt starter[J]. China Dairy Cattle,2017(9):50−57. doi: 10.19305/j.cnki.11-3009/s.2017.09.014
    [11]
    郭奇慧, 包志华. 应用模糊数学对搅拌型酸奶进行感官综合评价[J]. 中国乳品工业,2015,43(2):62−64. [GUO Qihui, BAO Zhihua. Improved sensory comprehensive evaluation method for stirred yogurt based on fuzzy mathemati[J]. China Dairy Industry,2015,43(2):62−64. doi: 10.3969/j.issn.1001-2230.2015.02.016
    [12]
    吴进菊, 于博, 陈婧, 等. 基于质构仪和电子舌分析市售发酵剂对酸奶滋味品质的影响[J]. 中国食品添加剂,2018(3):187−192. [WU Jinju, YU Bo, CHEN Jing, et al. Analysis of starter culture's effect on taste quality of yogurt based on texture analyser and electronic tongue[J]. China Food Additives,2018(3):187−192. doi: 10.3969/j.issn.1006-2513.2018.03.022
    [13]
    VAN V T, VAN V D, ZOON P, et al. Relation between syneresis and rheological properties of particle gels[J]. Colloid and Polymer Science,1991,269:620−627. doi: 10.1007/BF00659917
    [14]
    WANG X Y, ELEANA K, GISELE L. Adding apple pomace as a functional ingredient in stirred-type yogurt and yogurt drinks[J]. Food Hydrocolloids,2020,100(C):105453−105453. doi: doi.org/10.1016/j.food-hyd.2019.105453
    [15]
    JUSSARA K, LEDA B Q, JULIANA S, et al. Functional yogurt with strawberries and chia seeds[J]. Food Bioscience,2020,37. doi: doi.org/10.1016/j.fbio.2020.100726
    [16]
    JUAN O A, ANGELA P M, OSCAR V C, et al. Rheological, texture, structural, and functional properties of Greek-style yogurt fortified with cheese whey-spent coffee ground powder[J]. LWT,2020(129):109523.
    [17]
    汪秀妹, 汪毅宁, 胡玲芳, 等. 响应面法优化红枣酸奶配方及质构特性研究[J]. 食品研究与开发,2021,42(7):62−69. [WANG Xiumei, WANG Yining, HU Lingfang, et al. Optimization of the formula of red jujube yogurt by response surface methodology and its textural properties analysis[J]. Food Research and Development,2021,42(7):62−69. doi: 10.12161/j.issn.1005-6521.2021.07.011
    [18]
    曽羲. 搅拌型酸乳的流变学分析[J]. 轻工科技,2018,34(8):8−9. [ZENG Xi. Fluidological analysis of stirred yoghurt[J]. Light Industry Science and Technology,2018,34(8):8−9.
    [19]
    陈玮, 常忠义, 高红亮, 等. 不同发酵剂生产的酸奶感官评定和TPA质构分析[J]. 中国酿造,2012,31(1):193−195. [CHEN Wei, CHANG Zhongyi, GAO Hongliang, et al. Sensory evaluation and texture profile analysis of yogurt produced by different starter[J]. China Brewing,2012,31(1):193−195. doi: 10.3969/j.issn.0254-5071.2012.01.057
    [20]
    赵红玲, 李全阳, 赵正涛, 等. 搅拌型酸奶流变学特性的研究[J]. 乳品科学与技术,2009,32(4):159−163. [ZHAO Hongling, LI Quanyang, ZHAO Zhengtao, et al. Research on rheological pro-perties of stirred yogurt[J]. Dairy Science and Technology,2009,32(4):159−163.
    [21]
    任敏, 多拉娜, 王帅, 等. 基于电子鼻和电子舌技术评价乳酸乳球菌对酸奶风味品质的影响[J]. 中国食品学报,2021,21(1):246−255. [REN Min, DUO Lana, WANG Shuai, et al. Effect of Lactococcus lactis on flavor quality of fermented milk based on electronic tongue and electronic nose[J]. Journal of Chinese Institute of Food Science and Technology,2021,21(1):246−255.
    [22]
    SARA B, NAGHMEH H, SEYED S S. et al. Flaxseed mucilage: A natural stabilizer in stirred yogurt[J]. Carbohydrate Polymers,2018,187:59−65. doi: 10.1016/j.carbpol.2018.01.049
    [23]
    侯团伟, 段剑平, 吴晓红. 发酵剂在乳品中应用的影响因素及协同发酵的研究进展[J]. 食品工业科技,2021,42(1):378−386. [HOU Tuanwei, DUAN Jianping, WU Xiaohong. Recent progress of influence factors and synergistic fermentation of starters in dairy products[J]. Science and Technology of Food Industry,2021,42(1):378−386. doi: 10.13386/j.issn1002-0306.2020030171
    [24]
    黄艳娜, 游春苹, 刘振民. 保加利亚乳杆菌和嗜热链球菌相互作用的研究进展[J]. 乳业科学与技术,2016,39(6):25−28. [HUANG Yanna, YOU Chunping, LIU Zhenmin. Progress in understanding of the interaction between Lactobacillus bulgaricus and Streptococcus thermophilus[J]. Journal of Dairy Science and Technology,2016,39(6):25−28. doi: 10.15922/j.cnki.jdst.2016.06.006
    [25]
    田佳乐, 不同复配比例酸奶发酵剂的筛选极其发酵特性研究[D]. 内蒙古: 内蒙古农业大学, 2021.

    TIAN Jiale. Study on the screening and fermentation characteristics of yogurt starters with different compound proportions[D]. Inner Mongolia: Inner Mongolia Agricultural University, 2021.
    [26]
    胡盼盼, 宋微, 杜明, 等. 影响乳酸菌胞外多糖产量的因素[J]. 食品科技,2014,39(9):31−37. [HU Panpan, SONG Hui, DU Ming, et al. Factors contributing to the yield of exopolysaccharide by lactic acid bacteria[J]. Food Science and Technology,2014,39(9):31−37. doi: 10.13684/j.cnki.spkj.2014.09.008
    [27]
    林芳栋, 蒋珍菊, 廖珊, 等. 质构仪及其在食品品质评价中的应用综述[J]. 生命科学仪器,2009,7(5):61−63. [LIN Fangdong, JIANG Zhenju, LIAO Shan, et al. The summary of texture analyzer and its application in the evaluation for food quality[J]. Life Science Instruments,2009,7(5):61−63. doi: 10.3969/j.issn.1671-7929.2009.05.017
    [28]
    SETTACHAIMONGKON S, NOUT M J, AUTUNES F E C, et al. Influence of different proteolytic strains of Streptococcus thermophilus in co-culture with Lactobacillus delbrueckii subsp. bulgaricus on the metabolite profile of set-yoghurt[J]. International Journal of Food Microbiology,2014,177:29−36. doi: 10.1016/j.ijfoodmicro.2014.02.008
    [29]
    丛艳君, 易红, 郑福平. 基于电子舌技术区分酸奶的滋味[J]. 食品工业科技,2015,36(4):49−52. [CONG Yanjun, YI Hong, ZHENG Fuping. Taste distinguishing of yoghourts using electronic tongue[J]. Science and Technology of Food Industry,2015,36(4):49−52. doi: 10.13386/j.issn1002-0306.2015.04.001
    [30]
    马长路, 焦扬, 吴芸等. 富含低聚糖褐色酸奶的研制[J]. 中国乳品工业,2019,47(10):59−64. [MA Changlu, JIAO Yang, WU Yun, et al. Development of oligosaccharide-rich brown yoghurt[J]. China Dairy Industry,2019,47(10):59−64. doi: 10.3969/j.issn.1001-2230.2019.10.014
  • Cited by

    Periodical cited type(2)

    1. 谭婉静,张笑薇,白泓,李晓凤,余以刚,肖性龙. 丁香油与月桂酸对金黄色葡萄球菌的协同抑制作用. 现代食品科技. 2022(02): 278-285+265 .
    2. 于航,王海默,钟世勋,吕月琴,刘兆胜,孙汝江. 抗菌肽APSH-07与抗生素对常见致病菌的体外协同抗菌效果研究. 饲料工业. 2021(16): 22-26 .

    Other cited types(3)

Catalog

    Article Metrics

    Article views (618) PDF downloads (49) Cited by(5)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return