QIAN Yanfang, SHI Chenying, CHEN Guitang. Optimization of Ultrasound-Assisted Extraction and Decolorization Process of Polysaccharides from Mori fructus and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(16): 201−210. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110007.
Citation: QIAN Yanfang, SHI Chenying, CHEN Guitang. Optimization of Ultrasound-Assisted Extraction and Decolorization Process of Polysaccharides from Mori fructus and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(16): 201−210. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110007.

Optimization of Ultrasound-Assisted Extraction and Decolorization Process of Polysaccharides from Mori fructus and Its Antioxidant Activity

More Information
  • Received Date: November 01, 2021
  • Available Online: August 02, 2022
  • The ultrasound-assisted extraction, resin decolorization process and antioxidant activity of polysaccharides from Mori fructus (MFPs) were investigated in this work. The effects of ultrasonic extraction temperature, solid-liquid ratio, ultrasonic time and ultrasonic power on extraction yield of MFPs were investigated by single factor and orthogonal test. Taking decolorization rate as index, the effects of decolorization time, concentration of polysaccharide solution and decolorization temperature were investigated by single factor and orthogonal test. ABTS assay, DPPH assay, phenanthroline method and pyrogallol autoxidation reaction were used to evaluate the antioxidant activity in vitro. The results showed that the optimal ultrasonic extraction conditions were ultrasonic temperature 50 ℃, solid-liquid ratio 1:30 g/mL, ultrasonic time 70 min and ultrasonic power 500 W. Under these conditions, the extraction yield of MFPs was 4.59%±0.25%. The optimal decolorization conditions were decolorization time 5 h, concentration of MFPs solution 4 mg/mL and decolorization temperature 25 ℃. Under these conditions, the decolorization rate was 62.34%±1.27%. MFPs had strong scavenging ability on ABTS+ radical, DPPH radical, hydroxyl radical and superoxide anion radical with half inhibitory concentration (IC50) of 0.14, 0.68, 0.19 and 3.14 mg/mL, respectively.
  • [1]
    RAMAPPA V K, SRIVASTAVA D, SINGH P, et al. Mulberries: A promising fruit for phytochemicals, nutraceuticals, and biological activities[J]. International Journal of Fruit Science,2020,20(3):s1254−s1279.
    [2]
    CHEN C, YOU L J, ABBASI A M, et al. Optimization for ultrasound extraction of polysaccharides from mulberry fruits with antioxidant and hyperglycemic activity in vitro[J]. Carbohydrate Polymers,2015,130:122−132. doi: 10.1016/j.carbpol.2015.05.003
    [3]
    DONG Y H, CHEN C, HUANG Q, et al. Study on a novel spherical polysaccharide from Fructus mori with good antioxidant activity[J]. Carbohydrate Polymers,2021,256:117516. doi: 10.1016/j.carbpol.2020.117516
    [4]
    AI J, BAO B, BATTINO M, et al. Recent advances on bioactive polysaccharides from mulberry[J]. Food & Function,2021,12(12):5219−5235.
    [5]
    BHATTACHARJYA D, SADAT A, DAM P, et al. Current concepts and prospects of mulberry fruits for nutraceutical and medicinal benefits[J]. Current Opinion in Food Science,2021,40:121−135. doi: 10.1016/j.cofs.2021.03.009
    [6]
    WEN P, HU T G, LINHARDT R J, et al. Mulberry: A review of bioactive compounds and advanced processing technology[J]. Trends in Food Science & Technology,2019,83:138−158.
    [7]
    DENG Q, WANG X, CHEN H, et al. Structural characterization, modification and hepatoprotective effects of polysaccharide from Mori fructus[J]. International Journal of Biological Macromolecules,2020,153:357−363. doi: 10.1016/j.ijbiomac.2020.02.300
    [8]
    TAN X, CHEN H, ZHOU X. Study on the activity of Mori fructus polysaccharides and its derivatives against acute alcoholic liver injury in mice[J]. Journal of Carbohydrate Chemistry,2020,39(9):450−471. doi: 10.1080/07328303.2021.1895194
    [9]
    CHEN H, XIAO R, ZHOU X. Study on the extraction, purification, partial chemical characterization and anti-alcohol liver injury activity of Mori fructus polysaccharides[J]. New Journal of Chemistry,2020,44(46):20060−20070. doi: 10.1039/D0NJ00795A
    [10]
    BIAN L, CHEN H, ZHOU X. Untargeted lipidomics analysis of Mori fructus polysaccharide on acute alcoholic liver injury in mice using ultra performance liquid chromatography-quadrupole-orbitrap-high resolution mass spectrometry[J]. International Immunopharmacology,2021,97:107521. doi: 10.1016/j.intimp.2021.107521
    [11]
    LIU C J, LIN J Y. Anti-inflammatory and anti-apoptotic effects of strawberry and mulberry fruit polysaccharides on lipopolysaccharide-stimulated macrophages through modulating pro-/anti-inflammatory cytokines secretion and Bcl-2/Bak protein ratio[J]. Food and Chemical Toxicology,2012,50(9):3032−3039. doi: 10.1016/j.fct.2012.06.016
    [12]
    WANG D, LI H, LI B, et al. Systematic fractionation and immunoenhancement of water-soluble polysaccharides isolated from fruit of Morus alba L[J]. International Journal of Biological Macromolecules,2018,116:1056−1063. doi: 10.1016/j.ijbiomac.2018.05.106
    [13]
    JIAO Y, WANG X, JIANG X, et al. Antidiabetic effects of Morus alba fruit polysaccharides on high-fat diet and streptozotocin-induced type 2 diabetes in rats[J]. Journal of Ethnopharmacology,2017,199:119−127. doi: 10.1016/j.jep.2017.02.003
    [14]
    CHEN C, ZHANG B, FU X, et al. A novel polysaccharide isolated from mulberry fruits (Murus alba L.) and its selenide derivative: Structural characterization and biological activities[J]. Food & Function,2016,7(6):2886−2897.
    [15]
    李瑶, 李文林, 杨丽丽, 等. 桑椹在心血管疾病领域的药效实验研究现状分析与思考[J]. 中国中药杂志,2020,45(13):3055−3062. [LI Y, LI W L, YANG L L, et al. Status analysis and thinking on experimental study on efficacy of Mori fructus in treatment of cardiovascular diseases[J]. China Journal of Chinese Materia Medica,2020,45(13):3055−3062.

    LI Y, LI W L, YANG L L, et al. Status analysis and thinking on experimental study on efficacy of Mori fructus in treatment of cardiovascular diseases[J]. China Journal of Chinese Materia Medica, 2020, 45(13): 3055-3062.
    [16]
    王强, 王睿, 王存, 等. 桑葚多糖调节血糖代谢及体外抗氧化效果研究[J]. 食品科学,2014,35(11):260−264. [WANG Q, WANG R, WANG C, et al. Effects of mulberry polysaccharides on glucose metabolism and their antioxidant activities in vitro[J]. Food Science,2014,35(11):260−264. doi: 10.7506/spkx1002-6630-201411052

    WANG Q, WANG R, WANG C, et al. Effects of mulberry polysaccharides on glucose metabolism and their antioxidant activities in vitro[J]. Food Science, 2014, 35(11): 260-264. doi: 10.7506/spkx1002-6630-201411052
    [17]
    包海蓉, 李柏林, 阎冬妮, 等. 桑葚的开发利用与市场营销[J]. 食品科学,2004(S1):208−211. [BAO H R, LI B L, YAN D N, et al. Development, utilization and marketing of mulberry[J]. Food Science,2004(S1):208−211.

    BAO H R, LI B L, YAN D N, et al. Development, utilization and marketing of mulberry[J]. Food Science, 2004(S1): 208-211.
    [18]
    SILLERO L, PRADO R, LABIDI J. Simultaneous microwave-ultrasound assisted extraction of bioactive compounds from bark[J]. Chemical Engineering & Processing,2020,156:108100.
    [19]
    HOMA B, FARZIN Z A, AMIR F, et al. Comparisons between conventional, microwave- and ultrasound-assisted methods for extraction of pectin from grapefruit[J]. Chemical Engineering and Processing: Process Intensification,2011,50(11−12):1237−1243. doi: 10.1016/j.cep.2011.08.002
    [20]
    景荣琴, 熊清平, 景怡. 响应面法优化桑葚多糖的超声波辅助提取工艺条件[J]. 天然产物研究与开发,2014,26(4):570−574. [JING R Q, XIONG Q P, JING Y. Optimization of ultrasonic-assisted extraction of polysaccharides from Fructus mori by response surface methodology[J]. Natural Product Research and Development,2014,26(4):570−574.

    JING R Q, XIONG Q P, JING Y. Optimization of ultrasonic-assisted extraction of polysaccharides from Fructus mori by response surface methodology[J]. Natural Product Research and Development, 2014, 26(4): 570-574.
    [21]
    祝新媛. 桑葚多糖的提取纯化及其对溃疡性结肠炎小鼠保护作用研究[D]. 沈阳: 沈阳农业大学, 2020

    ZHU X Y. Extraction and purification of mulberry polysaccharide and its protective effect on ulcerative colitis in mice[D]. Shenyang: Shenyang Agricultural University, 2020.
    [22]
    夏玮, 吕庆, 张文清, 等. 大孔吸附树脂脱色桑叶多糖的研究[J]. 食品与发酵工业,2007(2):141−144. [XIA W, LÜ Q, ZHANG W Q, et al. Study on the decoloration of polysaccharides from mulberry leaves by macro-resin absorption[J]. Food and Fermentation Industries,2007(2):141−144. doi: 10.3321/j.issn:0253-990X.2007.02.032

    XIA W, LÜ Q, ZHANG W Q, et al. Study on the decoloration of polysaccharides from mulberry leaves by macro-resin absorption[J]. Food and Fermentation Industries, 2007(2): 141-144. doi: 10.3321/j.issn:0253-990X.2007.02.032
    [23]
    刘伟, 刘倩楠, 张良, 等. 草莓多糖树脂法脱色工艺优化及其化学性质研究[J]. 食品工业科技,2020,41(10):38−46,51. [LIU W, LIU Q N, ZHANG L, et al. Optimization of decoloration process by microporous resins and its chemical properties of strawberry polysaccharides[J]. Science and Technology of Food Industry,2020,41(10):38−46,51.

    LIU W, LIU Q N, ZHANG L, et al. Optimization of decoloration process by microporous resins and its chemical properties of strawberry polysaccharides[J]. Science and Technology of Food Industry, 2020, 41(10): 38-46, 51.
    [24]
    ZHEN B, CHEN X S, HAN D, et al. An alternative method for the decoloration of ɛ-poly-l-lysine eluate by macroporous resin in the separation and purification of ɛ-poly-l-lysine from fermentation broth[J]. Food and Bioproducts Processing,2015,95:332−338. doi: 10.1016/j.fbp.2014.10.006
    [25]
    王松柏, 秦雪梅, 郭小青, 等. 树脂对防风粗多糖脱色效果[J]. 应用化学,2005(12):1308−1311. [WANG S B, QIN X M, GUO X Q, et al. Decolorization of crude Saposhnikovia divaricata polysaccharide by resins[J]. Chinese Journal of Applied Chemistry,2005(12):1308−1311. doi: 10.3969/j.issn.1000-0518.2005.12.008

    WANG S B, QIN X M, GUO X Q, et al. Decolorization of crude Saposhnikovia divaricata polysaccharide by resins[J]. Chinese Journal of Applied Chemistry, 2005(12): 1308-1311. doi: 10.3969/j.issn.1000-0518.2005.12.008
    [26]
    LI X Y, WANG L. Effect of extraction method on structure and antioxidant activity of Hohenbuehelia serotina polysaccharides[J]. International Journal of Biological Macromolecules,2016,83:270−276. doi: 10.1016/j.ijbiomac.2015.11.060
    [27]
    CHEN X Y, SUN WATERHOUSE D X, YAO W Z, et al. Free radical-mediated degradation of polysaccharides: Mechanism of free radical formation and degradation, influence factors and product properties[J]. Food Chemistry,2021,365:130524. doi: 10.1016/j.foodchem.2021.130524
    [28]
    赵喜兰. 桑葚多糖提取、纯化分离及其降糖作用的研究[J]. 食品工业科技,2011,32(2):259−260. [ZHAO X L. Study on the purification and hypoglycemic effect of polysaccharide in mulberries[J]. Science and Technology of Food Industry,2011,32(2):259−260.

    ZHAO X L. Study on the purification and hypoglycemic effect of polysaccharide in mulberries[J]. Science and Technology of Food Industry, 2011, 32(2): 259-260.
    [29]
    LIAO D W, CHEN C, LIU J P, et al. Characterization and antitumor activities of polysaccharides obtained from ginger (Zingiber officinale) by different extraction methods[J]. International Journal of Biological Macromolecules,2020,152:894−903. doi: 10.1016/j.ijbiomac.2020.02.325
    [30]
    YANG J, TONG Y P, ZHU K M, et al. Optimization of mechanochemical-assisted extraction and decoloration by resins of polysaccharides from petals of Crocus sativus L[J]. Journal of Food Processing and Preservation,2018,42(1):e13369. doi: 10.1111/jfpp.13369
    [31]
    孙宁云, 姚欣, 张英慧, 等. 鸡蛋花多糖提取工艺优化及生物活性研究[J/OL]. 食品工业科技: 1−18[2021-12-02]. doi: 10.13386/j. issn1002-0306.2021050198.

    SUN N Y, YAO X, ZHANG Y H, et al. Optimization of extraction process of polysaccharides from Plumeria rubra L. cv. Acutifolia and evaluation of biological activities[J/OL]. Science and Technology of Food Industry: 1−18[2021-12-02]. doi: 10.13386/j.issn1002-0306.2021050198.
    [32]
    尹明松, 丁贺辉, 潘飞兵, 等. 响应面优化超声辅助双水相提取槟榔多糖及抗氧化活性研究[J]. 食品研究与开发,2021,42(19):163−170. [YIN M S, DING H H, PAN F B, et al. Optimization of ultrasonic-assisted aqueous two-phase extraction of Areca catechu L. polysaccharide using response surface design and assessment of its antioxidant activities[J]. Food Research and Development,2021,42(19):163−170. doi: 10.12161/j.issn.1005-6521.2021.19.023

    YIN M S, DING H H, PAN F B, et al. Optimization of ultrasonic-assisted aqueous two-phase extraction of Areca catechu L. polysaccharide using response surface design and assessment of its antioxidant activities[J]. Food Research and Development, 2021, 42(19): 163-170. doi: 10.12161/j.issn.1005-6521.2021.19.023
    [33]
    郑婷婷, 严亮, 张文杰, 等. 水碱连续提取黄皮疣柄牛肝菌粗多糖的理化性质及抗氧化活性研究[J]. 食品工业科技,2020,41(15):84−89. [ZHENG T T, YAN L, ZHANG W J, et al. Physicochemical properties and antioxidant activity of water-alkali continuous extraction of crude polysaccharides from Leccinellum crocipodium (Letellier.) watliag[J]. Science and Technology of Food Industry,2020,41(15):84−89.

    ZHENG T T, YAN L, ZHANG W J, et al. Physicochemical properties and antioxidant activity of water-alkali continuous extraction of crude polysaccharides from Leccinellum crocipodium (Letellier. ) watliag[J]. Science and Technology of Food Industry, 2020, 41(15): 84-89.
    [34]
    张莉, 柏红梅, 游敬刚, 等. 不同发酵剂菌种对蓝莓-桑葚复合酵素抗氧化活性的影响[J]. 食品科技,2021,46(6):29−34. [ZHANG L, BAI H M, YOU J G, et al. Effect of different fermentation strains on the antioxidant activity of blueberry-mulberry complex fermented liquor[J]. Food Science and Technology,2021,46(6):29−34.

    ZHANG L, BAI H M, YOU J G, et al. Effect of different fermentation strains on the antioxidant activity of blueberry-mulberry complex fermented liquor[J]. Food Science and Technology, 2021, 46(6): 29-34.
    [35]
    付金, 姚秋萍, 邓水秀, 等. 黔产皂角米多糖提取动力学及抗氧化活性研究[J]. 食品工业科技,2021,42(1):8−14. [FU J, YAO Q P, DENG S X, et al. Extraction kinetics and antioxidant activities of polysaccharides from seeds of Gleditsia sinensis in Guizhou[J]. Science and Technology of Food Industry,2021,42(1):8−14.

    FU J, YAO Q P, DENG S X, et al. Extraction kinetics and antioxidant activities of polysaccharides from seeds of Gleditsia sinensis in Guizhou[J]. Science and Technology of Food Industry, 2021, 42(1): 8-14.
    [36]
    陈红惠, 牛念拉姆. 底圩茶多糖的超声波辅助提取及其抗氧化活性[J]. 食品工业科技,2020,41(21):179−184. [CHEN H H, NIUNIAN L M. Ultrasonic extraction and antioxidant activity of polysaccharide from Dixu tea[J]. Science and Technology of Food Industry,2020,41(21):179−184.

    CHEN H H, NIUNIAN L M. Ultrasonic extraction and antioxidant activity of polysaccharide from Dixu tea[J]. Science and Technology of Food Industry, 2020, 41(21): 179-184.
    [37]
    何念武, 秦娇娇, 王新军. 超声辅助提取灰灰菜多糖工艺优化及其体外抗氧化活性[J]. 食品工业科技,2018,39(1):235−240,252. [HE N W, QIN J J, WANG X J. Optimization of ultrasonic assisted extraction technology of polysaccharide from Chenopodium album Linn and its antioxidant activity in vitro[J]. Science and Technology of Food Industry,2018,39(1):235−240,252.

    HE N W, QIN J J, WANG X J. Optimization of ultrasonic assisted extraction technology of polysaccharide from Chenopodium album Linn and its antioxidant activity in vitro[J]. Science and Technology of Food Industry, 2018, 39(1): 235-240, 252.
    [38]
    范秀萍, 吴红棉, 王娅楠, 等. 4种贝类糖胺聚糖体外清除自由基活性的比较[J]. 食品科技,2008(2):165−167. [FAN X P, WU H M, WANG Y N, et al. Free radical- scavenging activity of glycosaminoglycans from four seashells in vitro[J]. Food Science and Technology,2008(2):165−167. doi: 10.3969/j.issn.1005-9989.2008.02.048

    FAN X P, WU H M, WANG Y N, et al. Free radical- scavenging activity of glycosaminoglycans from four seashells in vitro[J]. Food Science and Technology, 2008(2): 165-167. doi: 10.3969/j.issn.1005-9989.2008.02.048
    [39]
    蔡延渠, 董碧莲, 陈利秋, 等. 桃胶多糖体内外抗氧化作用的研究[J]. 食品工业科技,2020,41(13):53−58. [CAI Y Q, DONG B L, CHEN L Q, et al. Antioxidant activity in vivo and in vitro of polysaccharide from peach gum[J]. Science and Technology of Food Industry,2020,41(13):53−58.

    CAI Y Q, DONG B L, CHEN L Q, et al. Antioxidant activity in vivo and in vitro of polysaccharide from peach gum[J]. Science and Technology of Food Industry, 2020, 41(13): 53-58.
    [40]
    VÁZQUEZ-RODRÍGUEZ B, GUTIÉRREZ-URIBE J, ANTUNES-RICARDO M, et al. Ultrasound-assisted extraction of phlorotannins and polysaccharides from Silvetia compressa (Phaeophyceae)[J]. Journal of Applied Phycology,2020,32(2):1441−1453. doi: 10.1007/s10811-019-02013-2
    [41]
    陈成. 五味子多糖提取工艺优化及其对α-葡萄糖苷酶抑制活性分析[J/OL]. 食品工业科技: 1−10[2021-12-02]. doi: 10.13386/j. issn1002-0306.2021090146.

    CHEN C. Optimization of extraction process of Schisandra chinensis polysaccharide and analysis of its inhibitory activity against α-glucosidase[J]. Science and Technology of Food Industry: 1−10[2021-12-02]. doi: 10.13386/j.issn1002-0306.2021090146.
    [42]
    王杉杉, 马韵升, 姚刚, 等. 超声波辅助复合酶法提取枸杞多糖工艺研究[J]. 中国酿造,2015,34(7):134−137. [WANG B B, MA Y S, YAO G, et al. Ultrasound-assisted compound enzyme extraction technology of polysaccharides from Lycium barbarum[J]. China Brewing,2015,34(7):134−137. doi: 10.11882/j.issn.0254-5071.2015.07.032

    WANG B B, MA Y S, YAO G, et al. Ultrasound-assisted compound enzyme extraction technology of polysaccharides from Lycium barbarum[J]. China Brewing, 2015, 34(7): 134-137. doi: 10.11882/j.issn.0254-5071.2015.07.032
    [43]
    SOROURIAN R, KHAJEHRAHIMI A, TADAYONI M, et al. Ultrasound-assisted extraction of polysaccharides from Typha domingensis: Structural characterization and functional properties[J]. International Journal of Biological Macromolecules,2020,160:758−768. doi: 10.1016/j.ijbiomac.2020.05.226
    [44]
    帅良, 廖玲燕, 段振华, 等. 百香果果皮多糖提取工艺优化及其抗氧化活性研究[J]. 食品工业科技,2020,41(18):150−156. [SHUAI L, LIAO L Y, DUAN Z H, et al. Optimization of extraction technology of polysaccharides from passion fruit peel and its antioxidant activity[J]. Science and Technology of Food Industry,2020,41(18):150−156.

    SHUAI L, LIAO L Y, DUAN Z H, et al. Optimization of extraction technology of polysaccharides from passion fruit peel and its antioxidant activity[J]. Science and Technology of Food Industry, 2020, 41(18): 150-156.
    [45]
    冉俊枫, 任艳, 田余波, 等. 苦笋壳多糖提取工艺及抗氧化活性研究[J]. 食品科技,2021,46(6):207−214. [RAN J F, REN Y, TIAN Y B, et al. Extraction optimization for polysaccharides from bamboo shoot shell of Pleioblastus amarus and the in vitro antioxidant activity[J]. Food Science and Technology,2021,46(6):207−214.

    RAN J F, REN Y, TIAN Y B, et al. Extraction optimization for polysaccharides from bamboo shoot shell of Pleioblastus amarus and the in vitro antioxidant activity[J]. Food Science and Technology, 2021, 46(6): 207-214.
    [46]
    沈晓静, 黄璐璐, 聂凡秋, 等. 云南小粒咖啡花多糖提取工艺优化及其抗氧化活性分析[J]. 食品工业科技,2022,43(4):8. [SHEN X J, HUANG L L, NIE F Q, et al. Study on optimization of extraction technology and antioxidant activity of polysaccharide from Yunnan arabica coffee flowers[J]. Science and Technology of Food Industry,2022,43(4):8.

    SHEN X J, HUANG L L, NIE F Q, et al. Study on optimization of extraction technology and antioxidant activity of polysaccharide from Yunnan arabica coffee flowers[J]. Science and Technology of Food Industry, 2022, 43(4): 8.
    [47]
    丁梁斌, 马春梅, 赵苹苹, 等. 响应面法优化酶解-超声辅助提取鸡血藤多糖工艺研究[J]. 中国食品添加剂,2021,32(7):88−96. [DING L B, MA C M, ZHAO P P, et al. Optimization of enzymatic hydrolysis-ultrasonic assisted extraction of polysaccharides from spatholobi by response surface methodology[J]. China Food Additives,2021,32(7):88−96.

    DING L B, MA C M, ZHAO P P, et al. Optimization of enzymatic hydrolysis-ultrasonic assisted extraction of polysaccharides from spatholobi by response surface methodology[J]. China Food Additives, 2021, 32(7): 88-96.
    [48]
    ZHU C P, ZHAI X C, LI L Q, et al. Response surface optimization of ultrasound-assisted polysaccharides extraction from pomegranate peel[J]. Food Chemistry,2015,177:139−146. doi: 10.1016/j.foodchem.2015.01.022
    [49]
    卫娜, 罗至钧, 郑逸蓝, 等. 超声-微波辅助提取板栗壳多糖及其结构鉴定[J]. 食品安全质量检测学报,2021,12(16):6600−6608. [WEI N, LUO Z J, ZHENG Y L, et al. Optimization of ultrasonic-microwave synergistic extraction process of chestnut shell polysaccharides and its structural identification[J]. Journal of Food Safety & Quality,2021,12(16):6600−6608.

    WEI N, LUO Z J, ZHENG Y L, et al. Optimization of ultrasonic-microwave synergistic extraction process of chestnut shell polysaccharides and its structural identification[J]. Journal of Food Safety & Quality, 2021, 12(16): 6600-6608.
    [50]
    YANG R, MENG D, SONG Y, et al. Simultaneous decoloration and deproteinization of crude polysaccharide from pumpkin residues by cross-linked polystyrene macroporous resin[J]. Journal of Agricultural and Food Chemistry,2012,60(34):8450−8456. doi: 10.1021/jf3031315
    [51]
    韩伟, 陈静雯. 应用大孔树脂提纯蛹虫草多糖的工艺研究[J]. 徐州工程学院学报(自然科学版),2021,36(2):7−14. [HAN W, CHEN J W. Study on purification of Cordyceps militaris polysaccharides by macroporous resin[J]. Journal of Xuzhou Institute of Technology (Natural Sciences Edition),2021,36(2):7−14.

    [HAN W, CHEN J W. Study on purification of Cordyceps militaris polysaccharides by macroporous resin[J]. Journal of Xuzhou Institute of Technology (Natural Sciences Edition), 2021, 36(2): 7-14.
    [52]
    SHI Y Y, LIU T T, HAN Y, et al. An efficient method for decoloration of polysaccharides from the sprouts of Toona sinensis (A. Juss.) roem by anion exchange macroporous resins[J]. Food Chemistry,2017,217:461−468. doi: 10.1016/j.foodchem.2016.08.079
    [53]
    段旭, 冉军舰, 孙俊良, 等. 甘薯渣多糖的提取工艺优化、结构鉴定及其功能活性研究[J]. 食品工业科技,2022,43(8):10. [DUAN X, RAN J J, SUN J L, et al. Study on extraction process optimization, structure identification and functional activity of polysaccharide from sweet potato residue[J]. Science and Technology of Food Industry,2022,43(8):10.

    DUAN X, RAN J J, SUN J L, et al. Study on extraction process optimization, structure identification and functional activity of polysaccharide from sweet potato residue[J]. Science and Technology of Food Industry, 2022, 43(8): 10.
  • Cited by

    Periodical cited type(17)

    1. 吕欣然,王淑娟,张丹,朱婷婷,孙翔宇,马婷婷. 不同剂量电子束辐照杀菌处理对黑果腺肋花楸果汁品质的影响. 食品科学. 2025(05): 272-280 .
    2. 兰天,赵沁雨,王家琪,孙翔宇,马婷婷. 益生菌发酵猕猴桃果汁的贮藏特性及货架期预测. 食品工业科技. 2024(05): 301-308 . 本站查看
    3. 张海军,李媛媛,钟祥静. 超高压灭菌技术在食品加工中的应用探讨. 粮油与饲料科技. 2024(02): 10-12 .
    4. 李媛媛,张海军,钟祥静. 基于超高压灭菌技术的农产品加工过程质量控制研究. 南方农机. 2024(17): 170-173 .
    5. 赵佳宇,易宗伟,蔡文超,马佳佳,王玉荣,单春会,郭壮. 动态超高压微射流技术对红枣酒品质的影响. 中国酿造. 2024(09): 147-151 .
    6. 程婧祺,秦雪,邱月,关宁,廖江,余志宝,裴晓燕,杨鑫焱,姜毓君,满朝新. 预测微生物学模型在乳及乳制品中的应用. 中国乳品工业. 2024(09): 50-55 .
    7. 张丽娟,邹波,肖更生,徐玉娟,余元善,吴继军,李璐. 不同打浆及杀菌处理对荔枝浆品质的影响. 食品工业科技. 2023(07): 329-336 . 本站查看
    8. 朱卫芳,黄兰淇,张颂函,马琳,陈建波,方朝阳. 25%吡唑醚菌酯悬浮剂在蓝莓中的残留行为及膳食风险评估. 农药科学与管理. 2023(01): 47-53 .
    9. 马琳,赵颖,陈建波,赵莉. 基于胶体金免疫层析法快速检测蓝莓中的百菌清残留. 农药学学报. 2023(02): 435-443 .
    10. 高惠颖,宋娟,景缘,于泳渤,张瑞,刘静,胡雨晴,吕长鑫,马志恒. NFC冻梨苹果汁配方优化及其贮藏品质. 食品研究与开发. 2023(11): 93-99 .
    11. 武正芳,马意龙,金诺,胡飞,章建国,魏兆军. 臭氧对食品加工中多酚影响的研究进展. 农产品加工. 2023(18): 79-82+92 .
    12. 赵倩,谢彦纯,赵冲. 百香果红茶饮料的研制. 中国果菜. 2023(12): 7-13 .
    13. 马琳,朱卫芳,占绣萍,陈建波,赵莉. 嘧霉胺在蓝莓中的残留行为及膳食风险评估. 农药学学报. 2022(04): 884-889 .
    14. 任博文,董璇,何珊. 超高压技术在食品应用中的研究进展. 农产品加工. 2022(16): 61-63+67 .
    15. 黄丽萍,靳学远,谭演清,陈涛,王华民. 超高压微射流处理对火龙果汁微生物指标及理化特性的影响. 食品安全质量检测学报. 2022(20): 6563-6568 .
    16. 宣晓婷,陈思媛,乐耀元,尚海涛,曾昊溟,凌建刚,张文媛. 高水分南美白对虾虾干货架期预测模型的构建. 农产品加工. 2022(19): 78-82+90 .
    17. 张丽娟,邹波,肖更生,徐玉娟,余元善,吴继军,温靖,李璐. 枸杞原浆低氧打浆联合不同杀菌技术的比较分析. 现代食品科技. 2022(11): 158-165 .

    Other cited types(18)

Catalog

    Article Metrics

    Article views (309) PDF downloads (41) Cited by(35)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return