Optimization of Ultrasound-Assisted Extraction and Decolorization Process of Polysaccharides from <i>Mori fructus</i> and Its Antioxidant Activity

QIAN Yanfang; SHI Chenying; CHEN Guitang

doi:10.13386/j.issn1002-0306.2021110007

Volume 43 Issue 16

Aug. 2022

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Science and Technology of Food Industry > 2022 > 43(16): 201-210. > DOI: 10.13386/j.issn1002-0306.2021110007

QIAN Yanfang, SHI Chenying, CHEN Guitang. Optimization of Ultrasound-Assisted Extraction and Decolorization Process of Polysaccharides from Mori fructus and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(16): 201−210. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021110007.

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Optimization of Ultrasound-Assisted Extraction and Decolorization Process of Polysaccharides from Mori fructus and Its Antioxidant Activity

College of Engineering, China Pharmaceutical University, National Traditional Chinese Medicine Processing Technology Research and Development Center, Nanjing 211198, China

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Received Date: November 01, 2021
Available Online: August 02, 2022

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Abstract

Abstract

The ultrasound-assisted extraction, resin decolorization process and antioxidant activity of polysaccharides from Mori fructus (MFPs) were investigated in this work. The effects of ultrasonic extraction temperature, solid-liquid ratio, ultrasonic time and ultrasonic power on extraction yield of MFPs were investigated by single factor and orthogonal test. Taking decolorization rate as index, the effects of decolorization time, concentration of polysaccharide solution and decolorization temperature were investigated by single factor and orthogonal test. ABTS assay, DPPH assay, phenanthroline method and pyrogallol autoxidation reaction were used to evaluate the antioxidant activity in vitro. The results showed that the optimal ultrasonic extraction conditions were ultrasonic temperature 50 ℃, solid-liquid ratio 1:30 g/mL, ultrasonic time 70 min and ultrasonic power 500 W. Under these conditions, the extraction yield of MFPs was 4.59%±0.25%. The optimal decolorization conditions were decolorization time 5 h, concentration of MFPs solution 4 mg/mL and decolorization temperature 25 ℃. Under these conditions, the decolorization rate was 62.34%±1.27%. MFPs had strong scavenging ability on ABTS⁺ radical, DPPH radical, hydroxyl radical and superoxide anion radical with half inhibitory concentration (IC₅₀) of 0.14, 0.68, 0.19 and 3.14 mg/mL, respectively.
- Mori fructus,
- polysaccharide,
- ultrasound-assisted extraction,
- decolorization,
- antioxidant activity

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