WANG Jinxiu, ZHAO Qingqing, XU Xinyi, et al. Optimization of Response Surface for Ultrasonic Acid Extraction of Pectin from Passion Fruit Peel and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(13): 162−170. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090363.
Citation: WANG Jinxiu, ZHAO Qingqing, XU Xinyi, et al. Optimization of Response Surface for Ultrasonic Acid Extraction of Pectin from Passion Fruit Peel and Its Antioxidant Activity[J]. Science and Technology of Food Industry, 2022, 43(13): 162−170. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090363.

Optimization of Response Surface for Ultrasonic Acid Extraction of Pectin from Passion Fruit Peel and Its Antioxidant Activity

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  • Received Date: October 10, 2021
  • Available Online: April 30, 2022
  • In order to further realize the waste utilization of PFP and reduce the waste of resources and environmental pollution, PFP pectin was extracted by ultrasonic assisted acid method and its antioxidant activity was studied. By investigating the effects of pH, solid-liquid ratio, ultrasonic time, ultrasonic power and ultrasonic temperature on the extraction ratio of PFP pectin, response surface optimization was carried out on the basis of single factor test to get the best extraction process, and acid method was used as the control. The effects of ultrasonic-assisted acid method on scavenging DPPH free radical (DPPH·), hydroxyl free radical (·OH) and superoxide anion free radical (O2·) of PFP pectin were studied. The results showed that: The optimal extraction parameters of PFP pectin by ultrasonic assisted acid method were pH2.0, ultrasonic time 70 min, ultrasonic power 210 W, ultrasonic temperature 45 ℃, and the yield of pectin was 14.78%±0.21%. The scavenging rates of DPPH·, ·OH and O2· were 60.96%±1.03%, 80.16%±1.78% and 59.13%±2.10%, which were higher than those of acid method.
  • [1]
    刘纯友, 殷朝敏, 黄永春, 等. 百香果皮多糖的分离纯化、结构特性及生物活性研究进展[J]. 食品工业科技,2018,39(8):335−340, 351. [LIU C Y, YIN C M, HUANG Y C, et al. Progress in extraction, separation and purification, structural characteristics and bioactivities of polysaccharides of passiflora edulis peel[J]. Science and Technology of Food Industry,2018,39(8):335−340, 351.

    LIU Chunyou, YIN Chaomin, HUANG Yongchun, et al. Progress in extraction, separation and purification, structural characteristics and bioactivities of polysaccharides of passiflora edulis peel [J]. Science and Technology of Food Industry, 2018, 39(8): 335-340, 351.
    [2]
    DA SILNA J K, CAZARIN C B B, BATISTA Â G, et al. Effects of passion fruit (Passiflora edulis) byproduct intake in antioxidant status of Wistar rats tissues[J]. LWT-Food Science and Technology,2014,59(2):1213−1219. doi: 10.1016/j.lwt.2014.06.060
    [3]
    唐语琪, 杨其军, 冼淑颜, 等. 百香果产业支撑乡村振兴的现状与对策建议[J]. 中国农业文摘-农业工程,2021,33(3):31−32, 60. [TANG Y Q, YNG Q J, XIAN S Y, et al. Current situation and countermeasures of passion fruit industry supporting rural revitalization[J]. Chinese Agricultural Abstracts-Agricultural Engineering,2021,33(3):31−32, 60. doi: 10.3969/j.issn.1002-5103.2021.03.012

    TANG Y Q, XIAN S Y, et al. Current situation and countermeasures of passion fruit industry supporting rural revitalization [J]. Chinese Agricultural Abstracts - Agricultural Engineering, 2021, 33(3): 31-32, 60. doi: 10.3969/j.issn.1002-5103.2021.03.012
    [4]
    龙倩倩. 西番莲果汁加工过程中香气成分变化 [D]. 广州: 华南农业大学, 2018.

    LONG Q Q. Changes of aroma components in processing of passionflower juice[D]. Guangzhou: South China Agricultural University, 2018.
    [5]
    NING X, LUO Z H, CHEN Z L, et al. Fortification of set yogurt with passion fruit juice: Effects on fermentation kinetics, physicochemical properties, and functionality[J]. Journal of Dairy Science,2021,104(4):4084−4093. doi: 10.3168/jds.2020-19261
    [6]
    ZHU X H, DUAN Z H, YANG Y X, et al. Development of passion fruit juice beverage[J]. IOP Conference Series: Earth and Environmental Science,2017,100(1):22−25.
    [7]
    康超, 杨玉霞, 冯珍, 等. 响应面法优化百香果醋发酵工艺及质量分析[J]. 中国酿造,2018,37(8):186−191. [KANG C, YANG Y X, FENG Z, et al. Optimization of fermentation technology for Passiflora edulis vinegar by response surface methodology and its quality analysis[J]. China Brewing,2018,37(8):186−191. doi: 10.11882/j.issn.0254-5071.2018.08.037

    KANG C, YANG Y X, FENG Z, et al. Optimization of fermentation technology for Passiflora edulis vinegar by response surface methodology and its quality analysis [J]. China Brewing, 2018, 37(8): 186-191. doi: 10.11882/j.issn.0254-5071.2018.08.037
    [8]
    程宏桢, 蔡志鹏, 王静, 等. 基于GC-MS、GC-O和电子鼻技术评价百香果酒香气特征[J]. 食品科学,2021,42(6):256−264. [CHEN H Z, CAI Z P, WANG J, et al. Combined use of GC-MS, GC-O and electronic nose technology to evaluate the aroma characteristics of passion fruit wine[J]. Food Science,2021,42(6):256−264. doi: 10.7506/spkx1002-6630-20200319-286

    CHEN H Z, CAI Z P, WANG J, et al. Combined Use of GC-MS, GC-O and electronic nose technology to evaluate the aroma characteristics of passion fruit wine [J]. Food Science, 2021, 42(6): 256-264. doi: 10.7506/spkx1002-6630-20200319-286
    [9]
    刘运花. 西番莲果皮中果胶的提取及性质研究 [D]. 广州: 华南农业大学, 2017.

    LIU Y H. Study on extraction and propertities of pectin from passiflora edulis rind[D]. Guangzhou: South China Agricultural University, 2017.
    [10]
    林增学. 百香果皮多糖的保健功能及生态旅游利用研究 [D]. 长沙: 中南林业科技大学, 2019.

    LIN Z X. Study on health care function and ecotourism utilization of passionfruit peel polysaccharide[D]. Changsha: Central South University of Forestry and Technology, 2019.
    [11]
    LÓPEZ-VARGAS J H, FERNÁNDEZ-LÓPEZ J, PÉREZ-ÁLVAREZl J A, et al. Chemical, physico-chemical, technological, antibacterial and antioxidant properties of dietary fiber powder obtained from yellow passion fruit (Passiflora edulis var. flavicarpa ) co-products[J]. Food Research International,2013,51(2):756−763. doi: 10.1016/j.foodres.2013.01.055
    [12]
    朱文娴, 夏必帮, 廖红梅. 西番莲的功能活性成分及加工与综合利用研究进展[J]. 食品与机械,2018,34(12):181−184. [ZHU W X, XIA B B, LIAO H M. Research progress on functional active components, processing and comprehensive utilization of passion flower[J]. Food and Machinery,2018,34(12):181−184.

    ZHU W X, XIA B B, LIAO H M. Research progress on functional active components, processing and comprehensive utilization of passion flower [J]. Food and Machinery, 2018, 34(12): 181-184.
    [13]
    应姗姗. 火龙果皮中果胶提取及改性研究 [D]. 杭州: 浙江大学, 2014.

    YING S S. Study on the extraction and modification of pectin from huolong peel[D]. Hangzhou: Zhejiang University, 2014.
    [14]
    王文骏. 柑橘皮果胶超声辅助提取的作用机制研究 [D]. 杭州: 浙江大学, 2018.

    WANG W J. Mechanism of ultrasound-assisted extraction of citrus peel pectin[D]. Hangzhou: Zhejiang University, 2018.
    [15]
    王雨晴, 刘畅, 杨春莉, 等. 食品中果胶提取及功能性研究进展[J]. 中国调味品,2020,45(8):175−177. [WANG Y Q, LIU C, YANG C L, et al. Research progress in the extraction and function of pectin in food[J]. China Condiment,2020,45(8):175−177. doi: 10.3969/j.issn.1000-9973.2020.08.039

    WANG Y Q, LIU C, YANG C L. et al. Research progress in the extraction and function of pectin in food [J]. China Condiment, 2020, 45(8): 175-177. doi: 10.3969/j.issn.1000-9973.2020.08.039
    [16]
    孙杨. 超声波辅助提取对绿茶提取液品质的影响 [D]. 合肥: 安徽农业大学, 2015.

    SUN Y. Effects of ultrasonic assisted extraction on the quality of green tea extract[D]. Hefei: Anhui Agricultural University, 2015.
    [17]
    MENG Q R, CHEN Z H, CHEN F, et al. Optimization of ultrasonic-assisted extraction of polysaccharides from Hemerocallis citrina and the antioxidant activity study[J]. Journal of Food Science,2021,86(7):3082−3096. doi: 10.1111/1750-3841.15806
    [18]
    TOMA M, VINATORU M, PANIWNYK L, et al. Investigation of the effects of ultrasound on vegetal tissues during solvent extraction[J]. Ultrasonics-Sonochemistry,2001,8(2):137−142. doi: 10.1016/S1350-4177(00)00033-X
    [19]
    TORKAMANI A E, JULIANO P, AJLOUNI S, et al. Impact of ultrasound treatment on lipid oxidation of cheddar cheese whey[J]. Ultrasonics-Sonochemistry,2014,21(3):951−957. doi: 10.1016/j.ultsonch.2013.11.021
    [20]
    OLIVEIRA C F D, GIORDANI D, LUTCKEMIER R, et al. Extraction of pectin from passion fruit peel assisted by ultrasound[J]. Food Science and Technology,2016,71:110−115.
    [21]
    黎英, 刘夏蕾, 林娅新, 等. 超声-微波协同提取百香果皮果胶的工艺研究[J]. 热带作物学报,2020,41(2):386−393. [LI Y, LIU X L, LIN Y X, et al. Study on ultrasonic-microwave assisted extraction of pectin from passion fruit peel[J]. Chinese Journal of Tropical Crops,2020,41(2):386−393. doi: 10.3969/j.issn.1000-2561.2020.02.025

    LI Y, LIU X L, LIN Y X, et al. Study on ultrasonic - microwave assisted extraction of pectin from passion fruit peel [J]. Chinese Journal of Tropical Crops, 2020, 41(2): 386-393. doi: 10.3969/j.issn.1000-2561.2020.02.025
    [22]
    严汉彬, 韩珍, 徐艳, 等. 超声波辅助酶法提取百香果皮中果胶的工艺研究[J]. 中国食品添加剂,2021,32(6):7−10. [YIN H B, HAN Z, XU Y, et al. Study on ultrasonic assisted enzymatic extraction of pectin from passion fruit peel[J]. China Food Additives,2021,32(6):7−10.

    YIN H B, HAN Z, XU Y, et al. Study on ultrasonic assisted enzymatic extraction of pectin from passion fruit peel [J]. China Food Additives, 2021, 32(6): 7-10.
    [23]
    辛明, 李昌宝, 李杰民, 等. 超声辅助柠檬酸提取百香果皮高酯果胶及其理化性质分析[J]. 食品工业科技,2021,42(4):115−120. [XIN M, LI C B, LI J M, et al. Optimization of extraction technology of high methoxyl pectin from passion fruit peel by ultrasound assisted with citric acid extraction and its physicochemical properties[J]. Science and Technology of Food Industry,2021,42(4):115−120.

    XIN M, LI C B, LI J M, et al. Optimization of extraction technology of high methoxyl pectin from passion fruit peel by ultrasound assisted with citric acid extraction and its physicochemical properties [J]. Science and Technology of Food Industry, 2021, 42(4): 115-120.
    [24]
    LIU J, BI J, MCCLEMENTS D J, et al. Impacts of thermal and non-thermal processing on structure and functionality of pectin in fruit- and vegetable- based products: A review[J]. Carbohydrate Polymers,2020,250:116890. doi: 10.1016/j.carbpol.2020.116890
    [25]
    SI Q X, YONG J Z, KAN J. Antioxidant activity in vitro and in vivo of the polysaccharides from different varieties of Auricularia auricula[J]. Food & Function,2016,7(9):3868−3879.
    [26]
    BARBOSA P D M, RUVIARO A R, MACEDO G A. Comparison of different Brazilian citrus by-products as source of natural antioxidants[J]. Food Science and Biotechnology,2018,27(5):1301−1309. doi: 10.1007/s10068-018-0383-4
    [27]
    RAHMANI Z, KHODAIYAN F, KAZEMI M, et al. Optimization of microwave-assisted extraction and structural characterization of pectin from sweet lemon peel[J]. International Journal of Biological Macromolecules,2020,147:1107−1115. doi: 10.1016/j.ijbiomac.2019.10.079
    [28]
    ENCALADA A M I, Pérez C D, FLORESl S K, et al. Antioxidant pectin enriched fractions obtained from discarded carrots (Daucus carota L.) by ultrasound-enzyme assisted extraction[J]. Food Chemistry,2019,289:453−460. doi: 10.1016/j.foodchem.2019.03.078
    [29]
    马丽苹, 焦昆鹏, 罗磊, 等. 改性苹果果胶性质及抗氧化活性[J]. 食品科学,2017,38(23):121−128. [MA L P, JIAO K P, LUO L, et al. Characterization and antioxidant activity of modified apple pectin[J]. Food Science,2017,38(23):121−128. doi: 10.7506/spkx1002-6630-201723020

    MA L P, JIAO K P, LUO L, et al. Characterization and antioxidant activity of modified apple pectin [J]. Food Science, 2017, 38(23): 121-128. doi: 10.7506/spkx1002-6630-201723020
    [30]
    宋佳敏, 王鸿飞, 孙朦, 等. 响应面法优化金蝉花多糖提取工艺及抗氧化活性分析[J]. 食品科学,2018,39(4):275−281. [SONG J M, WANG H F, SUN M, et al. Optimization of extraction and antioxidant activity of polysaccharides from Cordyceps cicadae by response surface methodology[J]. Food Science,2018,39(4):275−281. doi: 10.7506/spkx1002-6630-201804041

    SONG J M, WANG H F, SUN M, et al. Optimization of extraction and antioxidant activity of polysaccharides from Cordyceps cicadae by response surface methodology [J]. Food Science, 2018, 39(4): 275-281. doi: 10.7506/spkx1002-6630-201804041
    [31]
    陈丽叶, 常希光, 冯晓光, 等. 山药多糖的体外抗氧化活性研究[J]. 食品科学,2021,42(19):122−128. [CHEN L Y, CHANG X G, FENG X G, et al. In vitro antioxidant activity of Chinese yam polysaccharides[J]. Food Science,2021,42(19):122−128. doi: 10.7506/spkx1002-6630-20201107-073

    CHEN L Y, CHANG X G, FENG X G, et al. In vitro antioxidant activity of Chinese yam polysaccharides [J]. Food Science, 2021, 42(19): 122-128. doi: 10.7506/spkx1002-6630-20201107-073
    [32]
    刘晓霞. 黄秋葵花果胶类多糖的提取工艺及其性质的研究 [D]. 杭州: 浙江大学, 2014.

    LIU X X. Study on extraction technology and properties of pectin polysaccharide from okra flower[D]. Hangzhou: Zhejiang University, 2014.
    [33]
    LIEW S Q, CHIN N L, YUSOF Y A, et al. Comparison of acidic and enzymatic pectin extraction from passion fruit peels and its gel properties[J]. Journal of Food Process Engineering,2016,39(5):501−511. doi: 10.1111/jfpe.12243
    [34]
    宋思圆. 黄秋葵花多糖的超声提取及其结构和抗氧化活性研究 [D]. 杭州: 浙江大学, 2017.

    SONG S Y. Study on the structure and antioxidant activity of polysaccharides extracted by ultrasonography from sunflower flower[D]. Hangzhou: Zhejiang University, 2017.
    [35]
    BAYAR N, FRIJI M, KAMMOUN R. Optimization of enzymatic extraction of pectin from Opuntia ficus indica cladodes after mucilage removal[J]. Food Chemistry,2018,235:275−282.
    [36]
    VINATORU M. Ultrasonically assisted extraction (UAE) of natural products some guidelines for good practice and reporting[J]. Ultrasonics-Sonochemistry,2015,25:94−95. doi: 10.1016/j.ultsonch.2014.10.003
    [37]
    YING Z, HAN X X, LI J R. Ultrasound-assisted extraction of polysaccharides from mulberry leaves[J]. Food Chemistry,2011,127(3):1273−1279. doi: 10.1016/j.foodchem.2011.01.083
    [38]
    WAN P, YANG X M, CAI B N, et al. Ultrasonic extraction of polysaccharides from Laminaria japonica and their antioxidative and glycosidase inhibitory activities[J]. Oceanic and Coastal Sea Research,2015,14(4):651−662.
    [39]
    NIE J G, CHEN D T, LU Y. Deep eutectic solvents based ultrasonic extraction of polysaccharides from edible brown seaweed Sargassum horneri[J]. Journal of Marine Science and Engineering,2020,8(6):126−170.
    [40]
    MA L S, CHEN H X, ZHU W C, et al. Effect of different drying methods on physicochemical properties and antioxidant activities of polysaccharides extracted from mushroom Inonotus obliquus[J]. Food Research International,2013,50(2):633−640. doi: 10.1016/j.foodres.2011.05.005
    [41]
    FAN L P, LI J W, DENG K Q, et al. Effects of drying methods on the antioxidant activities of polysaccharides extracted from Ganoderma lucidum[J]. Carbohydrate Polyme,2012,87(2):1849−1854. doi: 10.1016/j.carbpol.2011.10.018
    [42]
    FLOEGEL A, KIM D O, CHUNG S J, et al. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods[J]. Journal of Food Composition and Analysis,2011,24(7):1043−1048. doi: 10.1016/j.jfca.2011.01.008
    [43]
    GONG G P, ZHAO J X, WANG C J, et al. Structural characterization and antioxidant activities of the degradation products from Porphyra haitanensis polysaccharides[J]. Process Biochemistry,2018,74:185−193. doi: 10.1016/j.procbio.2018.05.022
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