Study on Total Plate Count Detection in Beverage by 3M Petrifilm<sup>TM</sup> Rapid Aerobic Count Plate

ZHANG Wanjun; ZHANG Lan

doi:10.13386/j.issn1002-0306.2021040301

Volume 43 Issue 1

Dec. 2021

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Science and Technology of Food Industry > 2022 > 43(1): 311-319. > DOI: 10.13386/j.issn1002-0306.2021040301

ZHANG Wanjun, ZHANG Lan. Study on Total Plate Count Detection in Beverage by 3M Petrifilm^TM Rapid Aerobic Count Plate[J]. Science and Technology of Food Industry, 2022, 43(1): 311−319. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040301.

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Study on Total Plate Count Detection in Beverage by 3M Petrifilm^TM Rapid Aerobic Count Plate

Danone(China)Food & Beverage Co., Ltd., Zhongshan 528415, China

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Received Date: April 28, 2021
Available Online: November 05, 2021

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Abstract

Abstract

The 3M PetriFilm^TM rapid aerobic count plate method (3M test plate method) and filter membrane method were used to quantitatively determine the total number of bacterial colonies in the beverage, and the consistency of the two methods was analyzed. Seven kinds of bacteria including Escherichia coli, Staphylococcus aureus, Paenibacillus ehimensis, Bacillus subtilis, Asia Siamensis, Candida albicans and Aspergillus niger were used to artificially inoculate and test the beverage samples. The experimental results showed that for different strains and different pollution levels, the linear fitting degree of 3M test plate method and filter membrane method was better (determination coefficient R²>0.95). And the recovery rate of 3M test plate method was more than 70%, and the RSD values (2.9%~34.3%) were within the prescribed range of Pharmacopoeia of the People's Republic of China 2020, indicating that 3M test plate method had better accuracy and precision. For the specificity test of bacterial and fungal contamination and the repeatability test of different batches of 3M test plates, the recovery rate of 3M test plate method was more than 70%, and the RSD values (3.6%~34.3%) were within the acceptable range, showing good specificity and repeatability. The results of 3M test plate method and filter membrane method were analyzed by using relatively trueness and accuracy profileanalysis method. The results showed that the two methods had good consistency and equivalence. Based on the above analysis results, 3M test plate method is equivalent to filter membrane method to determine the total number of colonies in beverages.
- beverages,
- 3M Petrifilm^TM rapid aerobic count plate method,
- filter membrane method,
- aerobic count plate,
- method validation

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References (31)

References

[1]	范蕊, 王文文, 卢彬, 等. 食品中菌落总数、大肠菌群的检测技术探讨[J]. 现代食品,2021(4):212−214. [FAN Rui, WANG Wenwen, LU Bin, et al. Discussion on the detection technology of total number of colonies and coliform in food[J]. Modern Food,2021(4):212−214.
[2]	高翔, 马鹏飞, 崔亚宁. 食品微生物检验菌落总数测定方法的效果探讨[J]. 食品安全导刊,2021(3):94−95, 97. [GAO Xiang, MA Pengfei, CUI Yaning. The effect of the method for determination of the total number of bacterial colonies in food microbiological inspection[J]. Food Safety Guide,2021(3):94−95, 97.
[3]	刘凤会. 食品中菌落总数测定研究进展[J]. 食品安全导刊,2021(3):171. [LIU Fenghui. Research progress on determination of total number of bacterial colonies in food[J]. Food Safety Guide,2021(3):171.
[4]	张芬. 3种方法检测食品中菌落总数的比较研究[J]. 现代食品,2020(10):214−216. [ZHANG Fen. Comparative study on the total number of bacterial colonies in food by three methods[J]. Modern Food,2020(10):214−216.
[5]	中华人民共和国卫生部. GB 4789.2-2016 食品安全国家标准食品微生物学检验菌落总数测定[S]. 北京: 中国标准出版社, 2016. Ministry of Health, People's Republic of China. GB 4789.2-2016 National standard for food safety: Determination of total number of bacterial colonies in food microbiology[S]. Beijing: Standards Press of China, 2016.
[6]	中国饮料工业协会技术工作委员会. T/CBIA 005-2019饮料中微生物的检验(滤膜前处理法)[S]. 北京: 中国标准出版社, 2019. Techbical Working Committee of China Beverage Industry Association. T/CBIA 005-2019 Determination of microorganisms in beverages(filter membrane pretreatment method)[S]. Beijing: Standards Press of China, 2019.
[7]	徐红, 刘小波, 郭伟鹏, 等. 六株霉菌对维生素功能饮料理化性质的影响[J]. 现代食品科技,2015,31(10):263−268. [XU Hong, LIU Xiaobo, GUO Weipeng, et al. Effects of six strains of fungi on physical and chemical properties of vitamin energy drinks[J]. Modern Food Science and Technology,2015,31(10):263−268.
[8]	吴磊磊. 食品微生物快检技术的发展[J]. 粮食流通技术,2016(17):25−27. [WU Leilei. Development of rapid detection technology of food microorganism[J]. Grain circulation technology,2016(17):25−27.
[9]	PERIS-VICENTEJ, CA RDA-BROCH S, ESTEVE-ROMERO J. Validation of rapid microbiological methods[J]. Journal of Laboratory Automation,2015,20(3):259−64. doi: 10.1177/2211068214554612
[10]	马尚玄, 郭刚军, 黄克昌, 等. 不同分子量澳洲坚果多肽氨基酸组成与抑菌活性[J]. 食品工业科技,2021,42(7):83−88. [MA Shangxuan, GUO Gangjun, HUANG Kechang, et al. Amino acid composition and antimicrobial activity of macadamia polypeptides with different molecular weights[J]. Science and Technology of Food Industry,2021,42(7):83−88.
[11]	张兰, 徐红, 翁文川, 等. 流式细胞技术检测酸性饮料中菌落总数的研究[J]. 食品工业科技,2018,39(4):235−239. [ZHANG Lan, XU Hong, WENG Wenchuan, et al. Determination of total number of bacterial colonies in acidic beverages by flow cytometry[J]. Science and Technology of Food Industry,2018,39(4):235−239.
[12]	池福敏, 罗章, 辜雪冬, 等. 3M测试片法在西藏牦牛乳制品微生物检测中的应用[J]. 食品工业,2015,36(12):201−203. [CHI Fumin, LUO Zhang, GU Xuedong, et al. Application of 3M test plate method in microbial detection of Xizang yak dairy products[J]. Food Industry,2015,36(12):201−203.
[13]	张宏伟, 郑文杰, 赵宏, 等. AOAC食品微生物定量分析方法确认要求[J]. 食品工业科技,2007(4):241−243. [ZHANG Hongwei, ZHENG Wenjie, ZHAO Hong, et al. Requirements for validation of AOAC methods for the quantitative analysis of food microorganisms[J]. Food Science and Technology,2007(4):241−243. doi: 10.3969/j.issn.1002-0306.2007.04.073
[14]	国家药典委员会. 中华人民共和国药典, 三部[S]. 北京: 中国医药科技出版社, 2015: 216−218. Natinal Pharmacopoeia Commission. Pharmacopoeia of the People's Repunlic of China, Three[S]. Beijing: China Medical Science and Technology Press, 2015: 216−218.
[15]	PUM J. A practical guide to validation and verification of analytical methods in the clinical laboratory[J]. Advances in Clinical Chemistry,2019:215−281.
[16]	李辉, 杨晓莉, 刘立科, 等. 3种咪唑类抗真菌乳膏剂微生物限度检查方法学研究[J]. 药物评价研究,2021,44(4):721−727. [LI Hui, YANG Xiaoli, LIU Like, et al. Microbial limit test methodology of three imidazole antifungal creams[J]. Drug Evaluation Research,2021,44(4):721−727.
[17]	翟磊, 葛媛媛, 崔生辉, 等. ATP生物荧光增幅法在化妆品微生物检测中的验证研究[J]. 日用化学工业,2020,50(11):771−775,782. [ZHAI Lei, GE Yuanyuan, CUI Shenghui, et al. Validation of ATP bioluminescence enhancement method in microbial detection of cosmetics[J]. China Detergent Chemical Industry,2020,50(11):771−775,782. doi: 10.3969/j.issn.1001-1803.2020.11.006
[18]	吴琼, 姚东, 胡北, 等. 决明降脂灵咀嚼片的质量标准及微生物限度检查[J]. 西北药学杂志,2015,34(4):417−422. [WU Qiong, YAO Dong, HU Bei, et al. Study on the quality standard and microbial limit of CasinJiangzhiling chewable tablets[J]. Journal of Microbiology and Biotechnology,2015,34(4):417−422.
[19]	梁春梅, 喻东威, 吴玉秋, 等. 测试片法快速检测乳品中的菌落总数[J]. 中国乳品工业,2017,45(1):47−49. [LIANG Chunmei, YU Dongwei, WU Yuqiu, et al. Rapid detection of total number of bacterial colonies in milk by test plate method[J]. China Dairy Industry,2017,45(1):47−49. doi: 10.3969/j.issn.1001-2230.2017.01.012
[20]	苏晓燕, 杨菲, 刘万敏, 等. 测试片法在细菌菌落总数检测中的应用价值研究[J]. 人民军医,2019,62(7):628−631. [SU Xiaoyan, YANG Fei, LIU Wanmin, et al. Study on the application value of test plate method in the detection of total number of bacterial colonies[J]. People's Military Medical Journal,2019,62(7):628−631.
[21]	程苏云, 任锦玉, 童哲, 等. 实验室菌落计数精密度控制的研究——Ⅰ影响精密度的因素[J]. 中国食品卫生杂志,2001(5):11−13. [CHENG Suyun, REN Jinyu, TONG Zhe, et al. Study on precision control of laboratory colony count——Ⅰ factors affecting precision[J]. Chinese Journal of Food Hygiene,2001(5):11−13. doi: 10.3969/j.issn.1004-8456.2001.05.004
[22]	伏广伟, 张珍竹, 崔绮嫦, 等. 全自动菌落计数仪在纺织检测中的应用研究[J]. 中国纤检,2021(2):66−69. [FU Guangwei, ZHANG Zhenzhu, CUI Qichang, et al. Research on application of automatic colony counter in textile testing[J]. China Fiber Inspection,2021(2):66−69.
[23]	梁春梅, 常建军, 喻东威, 等. 自动菌落计数仪在乳品检测中的应用研究[J]. 食品安全质量检测学报,2020,11(1):146−152. [LIANG Chunmei, CHANG Jianjun, YU Dongwei, et al. Study on the application of automatic colony counting instrument in milk detection[J]. Journal of Food Safety and Quality,2020,11(1):146−152.
[24]	ISO. Microbiology of the food chain—Method validation—Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method[S]. 2016.
[25]	NÖ DOĞAN. Bland-Altman analysis: A paradigm to understand correlation and agreement[J]. Turkish Journal of Emergency Medicine,2018,18(4):139−141. doi: 10.1016/j.tjem.2018.09.001
[26]	MANSOURNIA M A, WATERS R, NAZEMIPOUR M, et al. Bland-Altman methods for comparing methods of measurement and response to criticisms[J]. Global Epidemiology,2020,3:100045.
[27]	CAO X, BAI Z, SUN C, et al. Bland–Altman analysis of different radiographic measurements of the hallux valgus angle and the intermetatarsal angle after distal osteotomy[J]. Orthopaedic Surgery,2020,12(6):1621−1626. doi: 10.1111/os.12759
[28]	OHNLUDBROOK J. Confidence in Altman–Bland plots: A critical review of the method of differences[J]. Clinical and Experimental Pharmacology and Physiology,2010,37(2):143−149. doi: 10.1111/j.1440-1681.2009.05288.x
[29]	MFEINBERG. Validation of analytical methods based on accuracy profiles[J]. Journal of Chromatography A, 2007, 1158 (1–2): 174−183.
[30]	HUBERT P, NGUYEN-HUU J J, BOULANGER B, et al. Validation of quantitative analytical procedure, Harmonization of approaches[J]. STP Pharma Pratiques,2003,13(3):101−138.
[31]	许金榜. VRBA和3M Petrifilm^TM快速测试片定量检测大肠菌群的性能比较研究[J]. 质量技术监督研究,2021(1):17−22. [XU Jinbang. Comparative study on the performance of VRBA and 3M PetriFilm^TM rapid aerobic count plate for quantitative detection of coliforms[J]. Research on Quality and Technical Supervision,2021(1):17−22. doi: 10.3969/j.issn.1674-5981.2021.01.004

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