Polyphenolic Compounds Analysis in Fruits of ‘Niurouhong’ Tangerine by UPLC-MS/MS

In this study, UPLC-MS/MS(Ultra Performance Liquid Chromatography Tandem Mass Spectrometry) was used to detect the polyphenolic compounds in mature fruits of three Guizhou local germplasms, named ‘Niurouhong’ tangerine(NRH), ‘Zhuhongju’(ZHJ) and ‘Hongxiangyou’(HXY). Results showed that a total of 493 compounds including flavonoids, phenolic acids, lignins and coumarins were detected, of which flavonoids(163) were the most, followed by phenolic acids(71). Generally, the results of heat map, volcano plot, differential metabolites selected, venn diagram and KEGG analysis showed that the types and relative contents of polyphenolic compounds were germplasm specific. The metabolites in NRH and ZHJ were more similar, and the number of different metabolites were the least. There were great differences in metabolite types and relative contents between the two and HXY, indicating that there was a close relationship between metabolite types and germplasm types. This study would be helpful to understand the accumulation characteristics of polyphenolic compounds in local citrus germplasm and provide reference for later development and utilization.