Optimization of Extraction Technology of Total Triterpenes from the Branches of <i>Actinidia arguta</i> by Response Surface Methodology and Its <i>in Vitro</i> Anti-inflammatory Activity

SUN Honglai; SHI Deyou; LI Lili; XIA Jun; Zaimenko N.V.; Skrypchenko N.V.; LIU Dejiang

doi:10.13386/j.issn1002-0306.2021010045

Volume 42 Issue 15

Jul. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(15): 189-197. > DOI: 10.13386/j.issn1002-0306.2021010045

SUN Honglai, SHI Deyou, LI Lili, et al. Optimization of Extraction Technology of Total Triterpenes from the Branches of Actinidia arguta by Response Surface Methodology and Its in Vitro Anti-inflammatory Activity[J]. Science and Technology of Food Industry, 2021, 42(15): 189−197. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010045.

Citation:

PDF (1728 KB)

Optimization of Extraction Technology of Total Triterpenes from the Branches of Actinidia arguta by Response Surface Methodology and Its in Vitro Anti-inflammatory Activity

SUN Honglai^{1, 2,},
SHI Deyou^{1, 2},
LI Lili^{1, 2},
XIA Jun^{1, 2},
Zaimenko N.V.^{2, 3},
Skrypchenko N.V.^{2, 3},
LIU Dejiang^{1, 2, ,}

1.
College of Life Sciences, Jiamusi University, Jiamusi 154007, China
2.
China-Ukraine Agriculture & Forestry Technology Development and Application International Cooperation Joint Lab, Jiamusi 154007, China
3.
M.M. Gryshko National Botanical Gardens of National Academy of Sciences of Ukraine, Kyiv 01014, Ukraine

More Information

Received Date: January 10, 2021
Available Online: May 31, 2021

Graphical Abstract

Abstract

Abstract

Objective: Using the branches of Actinidia argutaas the raw material to optimize the extraction technology of Total Triterpenes from the branches of Actinidia arguta by response surface methodology (RSM). The in vitro anti-inflammatory activity of AABTT was analyzed. Methods: 5% Vanillin-glacial acetic acid-perchloric acid method was used to quantitatively analyze AABTT. On the basis of single factor experiment, response surface methodology was applied to optimize the main extraction conditions. Using hyaluronidase activity and the inhibition of serum albumin denaturation as an anti-inflammatory evaluation index to evaluate the anti-inflammatory activity of AABTT. Results: The best conditions for the response surface optimization were as follows: ethanol concentration 77%, liquid-material ratio 40:1 mL/g, extracting time 40 minutes, and ultrasonic power 400 W. The extraction content of Total Triterpenes was (58.42±0.36) mg/g under the optimal conditions. The anti-inflammatory activity of AABTT (4 mg/mL) was 81.48%±0.48% for the hyaluronidase and 71.09%±0.39% for the denaturation of bovine serum albumin. Conclusion: The extraction conditions of the AABTT are optimized by response surface method, and it was preliminarily showed that the AABTT had significant anti-inflammatory activity. This result can provide a theoretical basis for the further exploitation and utilization of Actinidia arguta.
- Actinidia arguta branch,
- Total Triterpenes,
- response surface,
- extraction,
- anti-inflammatory activity

FullText(HTML)

References (26)

References

[1]	Li Y, Cui W, Wang R, et al. MicroRNA858-mediated regulation of anthocyanin biosynthesis in kiwifruit (Actinidia arguta) based on small RNA sequencing[J]. PloS One,2019,14(5):e0217480. doi: 10.1371/journal.pone.0217480
[2]	牛强, 申健, 刘悦, 等. 软枣猕猴桃主要活性成分及药理活性研究进展[J]. 食品工业科技,2019,40(3):333−338, 344.
[3]	Wojdyło A, Nowicka P, Oszmiański J, et al. Phytochemical compounds and biological effects of Actinidia fruits[J]. Journal of Functional Foods,2017,30:194−202. doi: 10.1016/j.jff.2017.01.018
[4]	He X, Fang J, Chen X, et al. Actinidia chinensis Planch. : A review of chemistry and pharmacology[J]. Frontiers in Pharmacology,2019,10:1236. doi: 10.3389/fphar.2019.01236
[5]	Wei L B, Ma S Y, Liu H X, et al. Cytotoxic Triterpenoids from roots of Actinidia chinensis[J]. Chemistry & Biodiversity,2018,15(2):e1700454.
[6]	王梦旭, 王天义, 白乃生. 软枣猕猴桃根化学成分及抗癌药理作用研究进展[J]. 中国中医药信息杂志,2019,26(9):137−140. doi: 10.3969/j.issn.1005-5304.2019.09.033
[7]	Fang T, Fang Y, Xu X, et al. Actinidia chinensis Planch root extract attenuates proliferation and metastasis of hepatocellular carcinoma by inhibiting epithelial-mesenchymal transition[J]. Journal of Ethnopharmacology,2019,231:474−485. doi: 10.1016/j.jep.2018.11.014
[8]	Zhang D, Gao C, Li R, et al. TEOA, a triterpenoid from Actinidia eriantha, induces autophagy in SW620 cells via endoplasmic reticulum stress and ROS-dependent mitophagy[J]. Archives of Pharmacal Research,2017,40(5):579−591. doi: 10.1007/s12272-017-0899-9
[9]	Cheng Q L, Li H L, Li Y C, et al. CRA (Crosolic acid) isolated from Actinidia valvata Dunn. Radix induces apoptosis of human gastric cancer cell line BGC823 in vitro via down-regulation of the NF-κB pathway[J]. Food and Chemical Toxicology,2017,105:475−485. doi: 10.1016/j.fct.2017.05.021
[10]	Cyboran-Mikołajczyk S, Csonka Á, Molnar J, et al. In vitro studies of anti-hemolytic and cytotoxic activity of procyanidin-rich extract from the leaves of Actinidiaarguta[J]. Polish Journal of Food and Nutrition Sciences,2018,68(2):171−177. doi: 10.1515/pjfns-2017-0021
[11]	Syed A S, Jeon J S, Kim C Y. A new diacetylated flavonol triglycoside from the aerial parts of Actinidia polygama[J]. Natural product research,2017,31(13):1501−1508. doi: 10.1080/14786419.2017.1278592
[12]	Teng K, Ruan H S, Zhang H F. Antinociceptive and anti-inflammatory effects of roots extracts from Actinidiaarguta (Sieb. et Zucc.) Planch[J]. Pharmacognosy Journal,2014,6(2).
[13]	Kim H Y, Hwang K W, Park S Y. Extracts of Actinidiaarguta stems inhibited LPS-induced inflammatory responses through nuclear factor–κB pathway in Raw 264.7 cells[J]. Nutrition research,2014,34(11):1008−1016. doi: 10.1016/j.nutres.2014.08.019
[14]	Meila O, Noraini N. Uji aktivitas antidiabetes dari ekstrak metanol buah kiwi (Actinidia deliciosa) melalui penghambatan aktivitas α-glukosidase[J]. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy),2017,3(2):132−137. doi: 10.22487/j24428744.0.v0.i0.8814
[15]	Jo Hee-Geun, Lee Geon-Yeong, Baek Chae Yun, et al. Aucklandia lappa analgesic and anti-inflammatory effects of root extracts on acetic acid-induced writhing in mice and monosodium iodoacetate-induced osteoarthritis in rats[J]. Plants (Basel),2020,10(1).
[16]	樊梓鸾, 赵梓煣, 赵翔, 等. 红豆越橘总三萜的纯化及体外抗炎活性[J]. 南京林业大学学报(自然科学版),2019,43(4):132−138.
[17]	Gan C Y, Latiff A A. Extraction of antioxidant pectic-polysaccharide from mangosteen (Garcinia mangostana) rind: Optimization using response surface methodology[J]. Carbohydrate Polymers,2011,83(2):600−607. doi: 10.1016/j.carbpol.2010.08.025
[18]	檀琪, 阮文辉, 杨官娥, 等. 响应面法优化桦褐孔菌总三萜回流提取工艺[J]. 山西医科大学学报, 2020, 51(1): 99-104.
[19]	MR de Melo M, MA Domingues R, JD Silvestre A, et al. Extraction and purification of triterpenoids using supercritical fluids: From lab to exploitation[J]. Mini-Reviews in Organic Chemistry,2014,11(3):362−381. doi: 10.2174/1570193X113106660002
[20]	Ruan W, Lim A H H, Huang L G, et al. Extraction optimisation and isolation of triterpenoids from Ganoderma lucidum and their effect on human carcinoma cell growth[J]. Natural product research,2014,28(24):2264−2272. doi: 10.1080/14786419.2014.938337
[21]	魏磊, 李晓, 王学方, 等. 紫苏叶总三萜超声提取工艺优化及对10种常见致病菌的抑菌作用研究[J]. 中国药房,2018,29(16):2193−2197. doi: 10.6039/j.issn.1001-0408.2018.16.08
[22]	Oludemi T, Barros L, Prieto M A, et al. Extraction of triterpenoids and phenolic compounds from Ganoderma lucidum: Optimization study using the response surface methodology[J]. Food & function,2018,9(1):209−226.
[23]	常霞, 景炳年, 范毅, 等. 金银花总三萜提取工艺及其抗菌抗氧化活性[J]. 江苏农业科学,2019,47(16):198−202.
[24]	Wei L, Zhang W, Yin L, et al. Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities[J]. Electronic Journal of Biotechnology,2015,18(2):88−95. doi: 10.1016/j.ejbt.2014.12.005
[25]	Gebretsadkan Hintsa Tekulu, Teref Hiluf, Hailu Brhanu, Ephrem Mebrahtu Araya, et al. Anti-inflammatory and anti-nociceptive property of Capparis tomentosa Lam. root extracts[J]. Journal of Ethnopharmacology,2020,253:112654. doi: 10.1016/j.jep.2020.112654
[26]	Isaac Tabiri Henneh, Robertson Akrofi, Elvis Ofori Ameyaw, et al. Stem bark extract of sterculia setigera delile exhibits anti-inflammatory properties through membrane stabilization, inhibition of protein denaturation and prostaglandin e2 activity[J]. Journal of Pharmaceutical Research International,2018:1−11.

[1]	CHEN Jiangkui, LI Jing, YIN Chunyan, LIAN Qimeng, YE Jia. Effects of Four Kinds of Antioxidants on the Oxidative Stability of Xanthoceras sorbifolia Bunge Oil and Analysis of Its Fatty Acid Compositions[J]. Science and Technology of Food Industry, 2022, 43(12): 70-76. DOI: 10.13386/j.issn1002-0306.2021090033
[2]	LI Yuan-yuan, PAN Ling, ZHANG Yan. Effects of natural antioxidants on the oxidative stability and flavor characteristics of peanut oil[J]. Science and Technology of Food Industry, 2018, 39(5): 241-249.
[3]	SONG Xiao-xiao, SUI Xiao-nan, MA Wen-jun, ZHANG Qiao-zhi, QI Bao-kun, JIANG Lian-zhou, LI Yang, LI Dan. Effect of rosemary extracts on the antioxidant capability of aqueous enzymatic vegetable oils[J]. Science and Technology of Food Industry, 2016, (02): 152-155. DOI: 10.13386/j.issn1002-0306.2016.02.022
[4]	ZHANG Qiao-zhi, LI Yang, QI Bao-kun, SUI Xiao-nan, MA Wen-jun, JIANG Lian-zhou. Study on the oxidation kinetics of vegetable oils by thermogravimetry[J]. Science and Technology of Food Industry, 2016, (02): 103-107. DOI: 10.13386/j.issn1002-0306.2016.02.012
[5]	WU Chen, CHEN Yi, MEI Jiang, ZHANG Zhi-hong, WANG Yu-ting. Studies of oxidative stability and the mechanisms of antioxidants in emulsions[J]. Science and Technology of Food Industry, 2015, (24): 380-384. DOI: 10.13386/j.issn1002-0306.2015.24.075
[6]	LI Jing, YAO Mao-jun, WANG Xu-dong, WANG Bang-yong, YANG Qing-fan. Study on auto-oxidation and anti-oxidation ability of peony seed oil[J]. Science and Technology of Food Industry, 2013, (22): 84-87. DOI: 10.13386/j.issn1002-0306.2013.22.019
[7]	Effect of several antioxidants on the oxidative stability of cotton seed oil[J]. Science and Technology of Food Industry, 2013, (11): 283-286. DOI: 10.13386/j.issn1002-0306.2013.11.034
[8]	Research progress in oxidative stability of vegetable oil[J]. Science and Technology of Food Industry, 2013, (07): 378-382. DOI: 10.13386/j.issn1002-0306.2013.07.048
[9]	Study on screening of efficient multiple antioxidants and stability of algal oil[J]. Science and Technology of Food Industry, 2012, (18): 318-321. DOI: 10.13386/j.issn1002-0306.2012.18.046
[10]	茶多酚对色拉油的抗氧化作用[J]. Science and Technology of Food Industry, 1999, (06): 27-28. DOI: 10.13386/j.issn1002-0306.1999.06.069

Supplements (0)

Cited By

Cited by

Periodical cited type(7)

1.	夏小雨，温财兴，曹文红，秦小明，李钰金，林海生，陈忠琴，郑惠娜，朱国萍，高加龙. 基于表面等离子体共振技术筛选马氏珠母贝肉酶解产物中血管紧张素转换酶抑制肽. 食品科学. 2025(07): 143-150 .
2.	吴靖娜，洪乔茜，廖榕榕，蔡水淋，陈晓婷，苏海燕，苏筱，许莉，潘南，卓诗晴. 红毛藻血管紧张素转化酶抑制肽的筛选及其稳定性评价. 食品科学. 2024(02): 188-194 .
3.	郭星晨，李玉豪，马金璞，张钰璇，李华鑫，杨具田，樊佩如，高丹丹. 基于氨基酸描述符对血管紧张素转化酶抑制五肽定量构效关系分析. 食品科学. 2024(13): 38-48 .
4.	钟玉旺，于梦怡，张丹，常莹，幸福兵，黄艾祥，王雪峰. 辣木籽凝乳酶在水牛乳干酪制备中应用及产血管紧张素转化酶抑制肽分析. 食品科学. 2024(18): 87-98 .
5.	王海东，张涵，周泓妍，史佳琳，石雨欣，衣春光，张红印，严铭铭. 响应面法优化五味子蛋白肽的制备工艺及其体外抗氧化活性. 食品工业科技. 2024(19): 166-176 . 本站查看
6.	杨志雨，王广慧，孟涓涓，郑晓宇，徐丽晶，韩昊霖. 响应面优化超声波辅助酶解法制备金针菇ACE抑制肽. 湖北农业科学. 2024(11): 153-159+167 .
7.	杨明哲，赵子莹，汤华成，李良玉，彭思念，李志江. 植物源咸味肽制备与应用研究进展. 食品工业科技. 2023(20): 467-474 . 本站查看