Construction of Engineering Bacteria for Transforming Phytosterol to 22-hydroxy-23, 24-bisnorchola-1, 4-diene-3-ketone(HPD) and Optimization of Fermentation Medium

MA Zhiguo; LIU Xiangcen; YUAN Chenyang; DU Guilin; NIU Bing; ZHANG Baoguo; SHI Jiping

doi:10.13386/j.issn1002-0306.2020120281

Volume 42 Issue 15

Jul. 2021

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Science and Technology of Food Industry > 2021 > 42(15): 131-138. > DOI: 10.13386/j.issn1002-0306.2020120281

MA Zhiguo, LIU Xiangcen, YUAN Chenyang, et al. Construction of Engineering Bacteria for Transforming Phytosterol to 22-hydroxy-23, 24-bisnorchola-1, 4-diene-3-ketone(HPD) and Optimization of Fermentation Medium[J]. Science and Technology of Food Industry, 2021, 42(15): 131−138. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120281.

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Construction of Engineering Bacteria for Transforming Phytosterol to 22-hydroxy-23, 24-bisnorchola-1, 4-diene-3-ketone(HPD) and Optimization of Fermentation Medium

1.
College of Life Sciences, Shanghai University, Shanghai 200444, China
2.
Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China
3.
Shanghai University of Science and Technology, Shanghai 201210, China

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Received Date: January 03, 2021
Available Online: May 30, 2021

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Abstract

As an important steroid intermediate, 22-hydroxy-23, 24-bisnorchola-1, 4-diene-3-ketone (HPD) was the raw material for the synthesis of many Sebaceous drugs.We overexpressed 3-ketosteroid-Δ¹-dehydrogenase (KstD) gene in Mycobacterium neoaurum DSM 1381, the purity of HPD in fermentation product was increased from 71% to 84%. On the basis of single factor selection, the yield of product HPD was taken as the measurement index, response surface method was used to optimize the fermentation medium, the quadratic regression equation for the change of each influencing factor was established. The final results showed that the most suitable fermentation medium composition conditions were corn steep liquor 9 g/L, NaNO₃1.8 g/L, glucose 6 g/L, and K₂HPO₄ 2 g/L.Under this condition, the yield of HPD could reach 3.73 g/L when the concentration of phytosterol was 5 g/L, and the yield of HPD was nearly 2.7 times higher than Mycobacterium neoaurum DSM 1381, it had potential industrial application value.

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