Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells

NI Jun; YUAN Cailian; SHE Rong; ZHAO Chengfa; LI Lijuan; YANG Xu; YANG Xiaoyan

doi:10.13386/j.issn1002-0306.2022050222

Volume 44 Issue 5

Feb. 2023

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Science and Technology of Food Industry > 2023 > 44(5): 60-66. > DOI: 10.13386/j.issn1002-0306.2022050222

NI Jun, YUAN Cailian, SHE Rong, et al. Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells[J]. Science and Technology of Food Industry, 2023, 44(5): 60−66. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050222.

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Effect of Cell Generations on t-BHP-induced Oxidative Stress Model of Caco-2 Cells

NI Jun^{1, 2,},
YUAN Cailian^{1, 2},
SHE Rong^{1, 2},
ZHAO Chengfa^{1, 2},
LI Lijuan^{1, 3},
YANG Xu¹,
YANG Xiaoyan^{1, 2, 4, 5, ,}

1.
Institute of Natural Antioxidants and Anti-inflammation, Dali University, Dali 671003, China
2.
Institute of Eastern-himalaya, Dali University, Dali 671003, China
3.
School of Public Health, Dali University, Dali 671003, China
4.
The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region from Yunnan Province, Dali 671003, China
5.
Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China, Dali 671003, China

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Received Date: May 18, 2022
Available Online: January 03, 2023

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Abstract

Objective: To investigate the effects of cell passage number on the establishment of an oxidative stress model in human colon adenocarcinoma cells (Caco-2) caused by tert-butyl hydroperoxide (t-BHP). Methods: First, by applying various t-BHP concentrations (1, 2 and 3 mmol/L) to the F15 generation of Caco-2 cells for 1, 2, 3, 4, 5 and 6 hours, the content of malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) in different treatment groups were measured to determine the basic conditions for constructing the oxidative stress model of Caco-2 cells. Then, based on the fundamental parameters, the effect of cell passage number (F14, F15, F18, F19 and F20) on the development of oxidative stress model was investigated using the content of 8-OHdG as an index. Results: When exposed to 1, 2 and 3 mmol/L of t-BHP for 3 to 6 hours, the oxidative stress model of Caco-2 cells from the F15 generation could be established steadily. The content of 8-OHdG in Caco-2 cells of the F14, F15 and F18 generations was significantly higher than that in control group (which had not been treated with t-BHP) after being exposed to 2.0 mmol/L t-BHP for 5 hours (P<0.05), whereas there was no significant difference between the F19 and F20 generations and the control group. Conclusion: The development of the oxidative stress model of Caco-2 cells will be strongly impacted by cell passage number. By subjecting Caco-2 cells prior to F19 to 2 mmol/L t-BHP for 5 hours, the oxidative stress model may be successfully constructed.
- Caco-2 cells,
- tert-butyl hydrogen peroxide,
- oxidative stress,
- cell model,
- cell passage number,
- 8-hydroxydeoxyguanosine

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