Isolation, Identification, Genetic Analysis and Evaluation of Germinating Fungi of <i>Gastrodia elata</i> Bl. in Non-Forestland of Changbai Mountain

LI Yaqi; GAO Yugang; ZANG Pu; LI Zhaochun

doi:10.13386/j.issn1002-0306.2020100004

Volume 42 Issue 15

Jul. 2021

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Science and Technology of Food Industry > 2021 > 42(15): 104-109. > DOI: 10.13386/j.issn1002-0306.2020100004

LI Yaqi, GAO Yugang, ZANG Pu, et al. Isolation, Identification, Genetic Analysis and Evaluation of Germinating Fungi of Gastrodia elata Bl. in Non-Forestland of Changbai Mountain[J]. Science and Technology of Food Industry, 2021, 42(15): 104−109. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020100004.

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Isolation, Identification, Genetic Analysis and Evaluation of Germinating Fungi of Gastrodia elata Bl. in Non-Forestland of Changbai Mountain

1.
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China
2.
Baishan Jingzhen Gastrodia Co., Ltd., Jingyu 135200, China

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Received Date: October 11, 2020
Available Online: May 27, 2021

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Abstract

Abstract

Objective: To obtain a good germinating strain of Gastrodia elata Bl. in Changbai Mountain.Methods: The germinating fungi were isolated by single mycelium separation, leaf separation and protocorm separation.The traditional morphology and molecular biology were used to identify germinating fungi, and the genetic diversity of germinating fungi was analyzed.Through the evaluation of the growth, morphological characteristics and germinating power of the germinating fungi, good strains were screened to promote the germination of Gastrodia elata Bl. in non-forest land of changbai mountain seeds.Results: Six closely related strains of Gastrodia elata Bl. germinating fungi were isolated.The homology of strains JFGL-01, JFGL-02, JFGL-03, JFGL-04, JFGL-05, JFGL-06 with Mycena purpureofusca were 98.86%, 99.54%, 99.84%, 98.99%, 99.09%, respectively;and 98%, 98.53%, 98.40%, 98.50%, 98.26%, 97.83% with Mycena citrinomarginata, respectively.The phylogenetic analysis showed that all strains were Mycena purpureofusca JFGL-06 's mycelia was white, dense, degenerated slowly, and strong in seed germination (The number of germinating protocorms was 59/100 cm², and the weight was 60.61 mg each). onclusion: JFGL-06 is a good germinating strain of Gastrodia elata Bl. in non-forest land of changbai mountain.
- Gastrodia elata Bl.f.glauca,
- germinating fungi,
- isolation and identification,
- genetic analysis,
- evaluation

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