Effects of Anthocyanins on Spectral Properties and Conformation of Bovine Serum Album

Xuhong ZHAO; Caifen XIA; Ziwei ZHOU; Wenli ZHUANG; Meiying GUO

doi:10.13386/j.issn1002-0306.2020060126

Volume 42 Issue 7

Mar. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(7): 57-62. > DOI: 10.13386/j.issn1002-0306.2020060126

ZHAO Xuhong, XIA Caifen, ZHOU Ziwei, et al. Effects of Anthocyanins on Spectral Properties and Conformation of Bovine Serum Album[J]. Science and Technology of Food Industry, 2021, 42(7): 57−62. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060126.

Citation:

PDF (1243 KB)

Effects of Anthocyanins on Spectral Properties and Conformation of Bovine Serum Album

College of Chemistry and Materials Science, Hubei Engineering University, Xiaogan 432000, China

More Information

Received Date: June 10, 2020
Available Online: January 27, 2021

Graphical Abstract

Abstract

Abstract

The effect of spectral properties and conformation of bovine serum album (BSA) by anthocyanin (ACN) was studied under simulated physiological conditions. Fluorescence spectroscopy was used to determine the quenching mode, number of binding sites, binding force type and non - radiative energy transfer between ACN and BSA. And BSA conformational changes were characterized by means of UV-Vis absorption spectrometry, circular dichroism spectroscopy and Fourier infrared absorption spectroscopy.The results showed that BSA endogenous fluorescence was quenched regularly with ACN, and the quenching mechanism was static quenching of ACN-BSA complex and non - radiative energy transfer. The quenching process was spontaneous by hydrophobic force, and the binding-site number was about 1. Furthermore, the binding sites were located in site 1 in the subdomain of BSA, and the α-helix of BSA was reduced by 3.1% in the presence of ACN.The interaction of ACN and BSA is strong, which causes a conformational change of BSA.
- anthocyanins,
- bovine serum albumin,
- fluorescence spectrometry,
- circular dichroism spectroscopy

FullText(HTML)

References (28)

References

[1]	王兵亚, 连秀仪, 杨琛擘, 等. 夏黑葡萄花青素抗氧化活性及对D-半乳糖衰老模型小鼠的保护作用[J]. 食品工业科技,2020,32(3):22−26.
[2]	姚惠芳, 景浩. 笃斯越橘花青素与牛血清白蛋白的相互作用[J]. 食品科学,2013,34(23):6−10.
[3]	周瑞, 董学艳, 景浩. 不同溶液中牛血清白蛋白与花青素相互作用特征及抗氧化性[J]. 食品科学,2013,34(15):11−16. doi: 10.7506/spkx1002-6630-201315003
[4]	扶晓菲, 鄢明辉, 游春苹. 原花青素生理功能及其与肠道微生物相互作用的研究进展[J]. 食品工业科技,2020,41(10):350−357.
[5]	崔灵敏, 谢笔钧, 孙智达. 果胶与莲原花青素复合物理化性质及体外抑菌活性研究[J]. 食品工业科技,2020,41(16):60−66, 80.
[6]	王晨, 谢岩黎, 范亭亭. 花青素与小麦蛋白相互作用及对蛋白质结构的影响[J]. 食品科学,2019,40(20):60−66. doi: 10.7506/spkx1002-6630-20180725-308
[7]	曾茜茜, 雷琳, 赵国华, 等. 花青素加工贮藏稳定性的改善及应用研究进展[J]. 食品科学,2018,39(11):269−275. doi: 10.7506/spkx1002-6630-201811042
[8]	郭亚琳, 刘瑞祥, 曹有福, 等. 花青素的提取及其在食品中的应用研究[J]. 安徽农业科学,2018,46(25):19−21. doi: 10.3969/j.issn.0517-6611.2018.25.006
[9]	彭斌, 李红艳, 邓泽元. 食品中花青素在热加工中的降解及其机制研究[J]. 食品安全质量检测学报,2016,7(10):3851−3858.
[10]	江连洲, 陈思, 李杨, 等. 大豆分离蛋白-花青素复合物的制备及其蛋白结构与功能性质分析[J]. 食品科学,2018,39(10):20−27. doi: 10.7506/spkx1002-6630-201810004
[11]	高义霞, 杨艳, 周向军. 3种不同溶剂中根皮苷与牛血红蛋白的相互作用及抗氧化性[J]. 食品工业科技,2020,41(19):78−84.
[12]	郭清莲, 潘凌立, 杨立云, 等. 姜黄素和去甲氧基姜黄素与牛血清白蛋白相互作用热力学行为[J]. 科学通报,2016,61(28-29):3137−3145.
[13]	金艳红, 陈会明, 何小英. 荧光光谱法研究酸性大红3R与牛血清白蛋白的结合作用[J]. 南昌大学学报,2009,33(4):205−209.
[14]	赵潇俊, 夏利华, 王孟可, 等. 荧光淬灭法考察小檗碱及衍生物与牛血清蛋白作用[J]. 药物生物技术,2018,25(3):206−209.
[15]	Yue Y Y, Sun Y Y, Dong Q, et al. Interaction of human serum albumin with novelimidazole derivatives studied by spectroscopy and molecular docking[J]. The Journal of Biological and Chemical Luminescence,2016,31(2):671−681.
[16]	Zhang G W, Ma Y D, Wang L, et al. Multispectroscopic studies on the interaction of maltol a food additive with bovine serum albumin[J]. Food Chemistry,2012,133:264−270. doi: 10.1016/j.foodchem.2012.01.014
[17]	王秀文, 王颖莉, 于茜, 等. 采用荧光光谱法研究DDAF与BSA的相互作用[J]. 中国测试,2019,45(7):80−86. doi: 10.11857/j.issn.1674-5124.2018110075
[18]	武嘉, 杨晓蕊, 王娜, 等. 荧光光谱法研究芥子碱与人血清蛋白的相互作用[J]. 大连大学学报,2013,34(3):58−61. doi: 10.3969/j.issn.1008-2395.2013.03.013
[19]	宋盼, 杨水兰, 周通, 等. 甘氨酸-N,N-双(亚甲基磷酸)及其Eu(Ⅲ)配合物与BSA的作用机制[J]. 中国稀土学报,2016,34(5):566−578.
[20]	Ross P D. Subramanian S, Themodynamics of protein association reactions forces contributing to stability[J]. Biochemistry,1981,20(11):3096−3102. doi: 10.1021/bi00514a017
[21]	朱国飞, 滕腾, 邓斌. 分子对接模拟与光谱法研究没食子酸丙酯和牛血清白蛋白的相互作用[J]. 食品工业科技,2016,37(18):158−164, 191.
[22]	薛燕斌, 乔华, 李波, 等. 光谱法研究高良姜素与人血清白蛋白的相互作用[J]. 食品工业科技,2017,38(18):65−68, 73.
[23]	杨美玲, 高琦宽, 宋玉民. 苦参碱Fe(III)化合物的合成及其与HSA相互作用的光谱研究[J]. 化学研究与应用,2014,26(10):1551−1556. doi: 10.3969/j.issn.1004-1656.2014.10.006
[24]	尚永辉, 李华, 孙家娟. 荧光光谱法研究原花青素与牛血清白蛋白的相互作用[J]. 分析科学学报,2011,27(2):179−182.
[25]	Zhang M, Li F, Wang J, et al. Spectorscopic and electrochemical studies on the interaction of an inclusion complex of β-cyclodextrin/fullerene with bovine serum albumin in aqueous solution[J]. Journal of Photochemical and Photobiology A: Chemistry,2012,1(15):28−37.
[26]	李道金. 荧光, 同步荧光, 三维荧光和圆二色谱法研究头孢吡肟盐酸盐与牛血清白蛋白的相互作用[J]. 贵州师范大学学报(自然科学版),2018,36(4):58−68.
[27]	武玉洁, 王丽欣, 封卓帆, 等. 大米蛋白质酰胺Ⅲ带三级红外光谱研究[J]. 湖南文理学院学报(自然科学版),2020,32(1):12−16.
[28]	武小明, 李丽慧, 霍嘉颖, 等. 降龙涎二醇与牛血清白蛋白的相互作用研究[J]. 现代食品科技,2019,35(5):124−130.

[1]	NIE Xuemei, XIE Yun, XU Bozhou, XU Xiuli. Differentiated Study of Constituents between Organic Milk and Ordinary Milk by Stable Isotope Ratio and UPLC-Q-Orbitrap[J]. Science and Technology of Food Industry, 2023, 44(23): 246-252. DOI: 10.13386/j.issn1002-0306.2022120095
[2]	ZHANG Shenping, HOU Chao, ZHANG Ni, DU Ruyun, ZHOU Jing, LIU Yang. Uncertainty Evaluation for Determination of 6 Sildenafil Derivatives in Oral Liquid by UPLC-Q/Orbitrap HRMS[J]. Science and Technology of Food Industry, 2023, 44(11): 280-286. DOI: 10.13386/j.issn1002-0306.2022060278
[3]	HE Zhuolin, MU Lei, WANG Tao, WANG Liang, XIE Yunfeng. Rapid Determination of 21 Mycotoxins in Milk and Dairy Products by Ultra Performance Liquid Chromatography-quadrupole/orbitrap High Resolution Mass Spectrometry[J]. Science and Technology of Food Industry, 2022, 43(6): 302-310. DOI: 10.13386/j.issn1002-0306.2021070077
[4]	REN Yuanyuan, WANG Chao, XIE Yong, GUO Guangpeng, HUANG Qinwan, WANG Jin. UPLC-Q-Orbitrap HRMS Combined with Network Pharmacology to Analyze the Material Basis and Mechanism of Schisandrae chinensis in the Treatment of Non-alcoholic Fatty Liver[J]. Science and Technology of Food Industry, 2022, 43(5): 21-33. DOI: 10.13386/j.issn1002-0306.2021090347
[5]	YANG Junlin, CHENG Pingyan, JIANG Lili, FENG Xiaobing, XIONG Xiaotong, YIN Yanyan, HU Feng, WANG Diqiang, ZHONG Fangda. Evaluation of Uncertainty in Determination of Ethyl Carbamate Residues in Chinese Baijiu by UPLC-Q/Orbitrap HRMS[J]. Science and Technology of Food Industry, 2021, 42(24): 239-246. DOI: 10.13386/j.issn1002-0306.2021030281
[6]	WEI Huan, LIU Jialing, LIAO Qiang. Rapid Screening and Determination of 20 Plant-Derived Toxins in Honey by UPLC-Q-Exactive Quadrupole-Electrostatic Field Track Trap High Resolution Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(17): 285-293. DOI: 10.13386/j.issn1002-0306.2020110234
[7]	YUAN Guangwei, WU Yi, WANG Haibo, MO Zimei, LIN Guangliao, JIANG Qiuxia. Determination of Eighteen Kinds of Free Amino Acids in Fruits by Ultra Performance Liquid Chromatography-Quadrupole-Exactive Orbitrap Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(5): 243-249. DOI: 10.13386/j.issn1002-0306.2020050015
[8]	LIU Shi-yao, WU Wen-lin, XIAO Quan-wei, LIANG Heng-xing, GOU Xing-hua. Rapid Screeninga nd Quantification of Capsaicin, Dihydrocapsaicin, Nonivamide in Edible Oil by Ultra-high Performance Liquid Chromatography-quadrupole/orbitrap Mass Spectroscopy[J]. Science and Technology of Food Industry, 2020, 41(13): 259-263. DOI: 10.13386/j.issn1002-0306.2020.13.041
[9]	XIE Bo, FU Hong, YANG Fang. Identification of Adulterated Honey by UPLC-Q-Exactive Quadrupole-Electrostatic Field Track Trap High Resolution Mass Spectrometry[J]. Science and Technology of Food Industry, 2020, 41(2): 244-251. DOI: 10.13386/j.issn1002-0306.2020.02.039
[10]	MO Ying, LV Min, ZHANG Rong-lin, XUE Ya-xin, HUANG Yan-hong, LU Xiao-kang, LIU Hua-wen. Determination of 15 Plant Growth Regulators Residues in Fruits and Vegetables by Modified QuEChERS Coupled with UPLC-Q-Orbitrap HRMS[J]. Science and Technology of Food Industry, 2020, 41(2): 195-200,206. DOI: 10.13386/j.issn1002-0306.2020.02.031

Supplements (0)

Cited By

Cited by

Periodical cited type(9)

1.	张明赞，贺彪，田辉，王媛. 酱香型白酒生产过程中微生物对白酒风味物质影响的研究进展. 农产品加工. 2024(06): 108-111 .
2.	高兴. 白酒品质的分析方法及质量安全控制策略. 食品安全导刊. 2024(28): 38-41 .
3.	马裕清，段万里，彭蕾，耿丙杰，唐凌瑄，王浩能，高方圆. 食品包装材料中双酚A检测方法的研究进展. 上海预防医学. 2023(06): 604-612 .
4.	孙震，郭铮蕾，刘莉，万晓楠，韩深，张朝晖，杨宇. 双酚A国内外管控法规和检测方法研究进展. 中国口岸科学技术. 2022(02): 38-44 .
5.	任媛媛，王超，谢勇，郭光鹏，黄勤挽，王瑾. 基于UPLC-Q-Orbitrap HRMS和网络药理学分析五味子治疗非酒精性脂肪肝的物质基础及作用机制. 食品工业科技. 2022(05): 21-33 . 本站查看
6.	周勇，高卓瑶，蒋玲波，贺文韬，汤海凤，陈毅洛，许景景，周小涵. 超高效液相色谱–串联质谱法同时测定化妆品中7种双酚类化合物及其衍生物. 化学分析计量. 2022(03): 73-77 .
7.	黄煜，李杰. 高效液相色谱法测定工业双酚A中的微量杂质. 化学试剂. 2022(08): 1213-1217 .
8.	张慧，曹慧，姜侃，肖功年，王瑾，周学军，厉小燕. 高效液相色谱-三重四极杆串联质谱法研究塑料接触材料中酚类化合物向奶油中的迁移. 食品科学. 2022(18): 339-345 .
9.	王庆亮. 白酒品质分析及质量安全控制研究进展. 酿酒科技. 2021(08): 97-101 .