Preparation and Optimization of Modified Chitosan Composite Film

Jinxing LI; Chunhong SHEN; Xianfa LI

doi:10.13386/j.issn1002-0306.2020060067

Volume 42 Issue 8

Apr. 2021

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Science and Technology of Food Industry > 2021 > 42(8): 144-151. > DOI: 10.13386/j.issn1002-0306.2020060067

LI Jinxing, SHEN Chunhong, LI Xianfa. Preparation and Optimization of Modified Chitosan Composite Film[J]. Science and Technology of Food Industry, 2021, 42(8): 144−151. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060067.

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Preparation and Optimization of Modified Chitosan Composite Film

College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621000, China

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Received Date: June 07, 2020
Available Online: January 27, 2021

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Abstract

Abstract

In this work, the thiolated chitosan was prepared by modification of chitosan. The composite film was prepared with thiolated chitosan as the film-forming matrix, adding gellan gum, glycerin, calcium chloride and natamycin as modifier. Based on the tensile strength, elongation at break, water vapor transmittance and transmittance of the film, the optimum addition amount of these four substances were determined by single factor experiments. By the Plackett-Burman experiment and the steepest climb experiment, the factors that had significant influence on the tensile strength of the film and the optimal experiment range were determined. Finally, the response surface experiment was carried out, and the tensile strength was taken as the evaluation index. The secondary response prediction model was obtained, and the optimal ratio of composite film was optimized. The results showed that the tensile strength of the single chitosan film was 0.928 MPa, but the elongation at break was only 5.91%. The tensile strength of the single thiolated chitosan film was only 0.350 MPa, and the elongation at break was 14.47%. When the basic film solution contained 0.20g of thiolated chitosan, the modified conditions were 0.18 g gellan gum, 1.00 g glycerol, 0.17 g calcium chloride and 0.01 g natamycin, the tensile strength of the composite film reached the maximum of 4.986±0.087 MPa. Under these, the tensile strength at break of the modified chitosan composite film was obviously (P<0.05) improved. The results of this study would provide theoretical support for the preparation of modified chitosan composite film, the improvement of composite properties and its application in the field of food preservation.
- thiolated chitosan,
- Plackett Burman design,
- response surface method,
- composite film

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References (34)

References

[1]	梁丽媚, 李思东, 李程鹏, 等. 壳聚糖及其衍生物抗菌活性的研究进展[J]. 广州化工,2017,45(20):6−9. doi: 10.3969/j.issn.1001-9677.2017.20.004
[2]	Weller C, Sussman G. Wound dressings update[J]. Journal of Pharmacy Practice and Research,2006,36(4):318−324. doi: 10.1002/j.2055-2335.2006.tb00640.x
[3]	汪冬梅, 邓体瑛. 巯基化聚合物的研究进展[J]. 药物生物技术,2016,23(1):91−94.
[4]	Wang G, Lu G, Ao Q, et al. Preparation of cross-linked carboxymethyl chitosan for repairing sciatic nerve injury in rats[J]. Biotechnology Letters,2010,32(1):59−66. doi: 10.1007/s10529-009-0123-1
[5]	Leitner V M, Walker G F, Bernkop-Schnürch A. Thiolated polymers: Evidence for the formation of disulphide bonds with mucus glycoproteins[J]. European Journal of Pharmaceutics & Biopharmaceutics,2003,56(2):207−214.
[6]	Roldo M, Hornof M, Caliceti P, et al. Mucoadhesive thiolated chitosans as platforms for oral controlled drug delivery: Synthesis and in vitro evaluation[J]. European Journal of Pharmaceutics & Biopharmaceutics,2004,57(1):115−121.
[7]	Kast C E, Bernkop-Schnürch A. Thiolated polymers--thiomers: Development and in vitro evaluation of chitosan-thioglycolic acid conjugates[J]. Biomaterials,2001,22(17):2345−2352. doi: 10.1016/S0142-9612(00)00421-X
[8]	Kafedjiiski K, Krauland A H, Hoffer M H, et al. Synthesis and in vitro evaluation of a novel thiolated chitosan[J]. Biomaterials,2005,26(7):819−826. doi: 10.1016/j.biomaterials.2004.03.011
[9]	王凯丽. 巯基壳聚糖衍生物的制备及其性质研究[D]. 济南: 济南大学, 2013.
[10]	王坤. 巯基化壳聚糖脂质体水凝胶的制备及其生物相容性研究[D]. 广州: 暨南大学, 2017.
[11]	张艳飞. 改性壳聚糖在樱桃保鲜剂中的应用研究[J]. 中小企业管理与科技(下旬刊),2015(1):324−325.
[12]	周正光. 海藻酸钠—琼胶—结冷胶复合膜的制备、储藏及应用[D]. 青岛: 中国海洋大学, 2014.
[13]	康丁, 张洪斌, 西成胜好. 生物医用结冷胶及其改性水凝胶材料[J]. 化学进展,2014,26(7):1172−1189.
[14]	朱桂兰, 叶银杉, 葛洁, 等. 低酰基结冷胶-果胶复配体系的性能[J]. 食品科学,2017(13):72−76.
[15]	Zia K M, Tabasum S, Khan M F, et al. Recent trends on gellan gum blends with natural and synthetic polymers: A review[J]. International Journal of Biological Macromolecules,2017:S0141813017326752.
[16]	Shukla R, Kashaw S K, Jain A P, et al. Fabrication of Apigenin loaded gellan gum–chitosan hydrogels (GGCH-HGs) for effective diabetic wound healing[J]. International Journal of Biological Macromolecules,2016,91:1110−1119. doi: 10.1016/j.ijbiomac.2016.06.075
[17]	Liu L, Wang B, Gao Y, et al. Chitosan fibers enhanced gellan gum hydrogels with superior mechanical properties and water-holding capacity[J]. Carbohydrate Polymers,2013,97(1):152−158. doi: 10.1016/j.carbpol.2013.04.043
[18]	颜田田, 戚勃, 杨贤庆, 等. 增塑剂对卡拉胶可食用膜性能的影响[J]. 食品与发酵工业,2019,45(23):97−102.
[19]	徐云升. 可食用膜的改性研究[J]. 食品工业科技,2002(10):72−75. doi: 10.3969/j.issn.1002-0306.2002.10.027
[20]	李金龙. 采后不同浓度钙处理对贮藏期间李果实硬度及果胶含量的影响[J]. 中国林副特产,2015(2):20−22.
[21]	余俊, 赵玉华, 白描, 等. 钙处理对葡萄果实花青素含量及品质的影响[J]. 果树学报,2018,35(6):741−746.
[22]	王玲利, 林春来, 韦洁敏, 等. 钙处理对黄冠梨贮藏品质的影响[J]. 中国园艺文摘,2018,34(6):27−29. doi: 10.3969/j.issn.1672-0873.2018.06.008
[23]	郭红彦, 白晋华, 段风琴, 等. 钙处理对‘壶瓶枣’裂果细胞壁降解酶活性及组织结构的影响[J]. 园艺学报,2019,46(8):1486−1494.
[24]	宋雪健, 张东杰, 王洪江, 等. 天然生物抗菌剂纳他霉素在食品中的应用及研究进展[J]. 保鲜与加工,2017,17(5):129−135.
[25]	刘丽梅. 壳聚糖纤维/结冷胶复合水凝胶的力学性质和保水性[D]. 苏州: 苏州大学, 2013.
[26]	杨飞. 壳聚糖—结冷胶钙凝胶珠的制备与释放特性研究[D]. 无锡: 江南大学, 2013.
[27]	徐幸, 童群义. 基于明胶硬胶囊性能评价的明胶—普鲁兰复合膜研究[J]. 食品与机械,2019,35(5):137−143.
[28]	Aguayo E, Escalona V H, Artés F. Effect of hot water treatment and various calcium salts on quality of fresh-cut ‘Amarillo’ melon[J]. Postharvest Biology and Technology,2008,47(3):397−406. doi: 10.1016/j.postharvbio.2007.08.001
[29]	甘祥武, 胡海艳, 黄魁英, 等. 响应面法优化甲硫氨酸γ-裂解酶发酵培养基[J]. 食品工业科技,2018,39(5):113−118.
[30]	李梦琦, 汪东风, 高翔, 等. 结冷胶琼胶可食性复合膜的研究[J]. 食品工业科技,2012,33(14):319−322.
[31]	Thakhiew W, Devahastin S, Soponronnarit S. Effects of drying methods and plasticizer concentration on some physical and mechanical properties of edible chitosan films[J]. Journal of Food Engineering,2010,99(2):216−224. doi: 10.1016/j.jfoodeng.2010.02.025
[32]	Nazan Turhan K, Şahbaz F. Water vapor permeability, tensile properties and solubility of methylcellulose-based edible films[J]. Journal of Food Engineering,2004,61(3):459−466. doi: 10.1016/S0260-8774(03)00155-9
[33]	王君, 乔翼娇, 胡文. 可食性壳聚糖膜的制备及功能特性研究[J]. 包装与食品机械,2019,37(4):15−18. doi: 10.3969/j.issn.1005-1295.2019.04.004
[34]	赵郁聪, 靳刘萍, 陈满儒, 等. 甘油对壳聚糖-淀粉复合可食性膜力学性能的影响[J]. 包装工程,2019,40(21):68−73.

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