XU Wei, MA Zhiyu, LI Jiamei, CHEN Hua, FAN Hongchen, ZHANG Guang. Optimization of Microwave-assisted Extraction of Quercetin from Loquat Flowers by Response Surface Methodology and the Effects on Activity of Alcoholic Intoxication Enzyme[J]. Science and Technology of Food Industry, 2021, 42(3): 127-132,139. DOI: 10.13386/j.issn1002-0306.2020040244
Citation: XU Wei, MA Zhiyu, LI Jiamei, CHEN Hua, FAN Hongchen, ZHANG Guang. Optimization of Microwave-assisted Extraction of Quercetin from Loquat Flowers by Response Surface Methodology and the Effects on Activity of Alcoholic Intoxication Enzyme[J]. Science and Technology of Food Industry, 2021, 42(3): 127-132,139. DOI: 10.13386/j.issn1002-0306.2020040244

Optimization of Microwave-assisted Extraction of Quercetin from Loquat Flowers by Response Surface Methodology and the Effects on Activity of Alcoholic Intoxication Enzyme

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  • Received Date: April 20, 2020
  • Available Online: February 02, 2021
  • Quercetin was extracted from loquat flower dried in shade by microwave-assisted extraction with 60% ethanol as solvent.The effects of material-liquid ratio,microwave time and microwave power on quercetin extraction from loquat flower were studied.The Box-Behnken response surface method was used to optimize the extraction process by three factors and three levels.The results indicated that the optimum extraction conditions were solid-liquid ratio of 1∶15 (g/mL),microwave time of 25 min and microwave extraction power of 600 W;the extraction amount of quercetin from loquat flower was 8.26 mg/g. Quercetin from loquat flower was purified by HP-20 column chromatography,the purity of quercetin obtained was 64.87%. Infrared spectrum scans indicated that quercetin characteristic peaks existed at 1663,1611 and 1382 cm-1.The effects of quercetin from loquat flower on the activities of ADH,ALDH and CAT in the process of alcoholic intoxication were investigated. The results for the test showed that the enzyme activities were all improved to a certain extent and the activation rates were 24.83%,29.18%,22.36%. Quercetin from loquat flower has certain activation effect on key enzymes in alcohol intoxication process,which provides certain theoretical basis for the development of natural product anti-alcoholism and food of auxiliary liver protection.
  • [1]
    应跃跃,张蕾蕾,王喜周.枇杷花利用价值和产品的综合开发[J].现代园艺,2013(8):171-172.
    [2]
    郭文场,刘佳贺,李悦.中国枇杷属种质资源、品种和栽培管理(1)[J].特种经济动植物,2017,20(9):49-52.
    [3]
    文卫华.枇杷疏花疏果技术及对产量品质影响研究[J].湖南林业科技,2007,34(6):17-19.
    [4]
    杨必坤.枇杷花药材质量标准研究[D].成都:四川师范大学,2010.
    [5]
    姜帆,周丹蓉,高慧颖,等.枇杷种质花中黄酮含量分析与评价[J].植物遗传资源学报,2016,17(6):1031-1035.
    [6]
    Bischoff S C.Quercetin:potentials in the prevention and therapy of disease[J].Current Opinion in Clinical Nutritionand Metabolic Care,2008,11(6):733-740.
    [7]
    马纳,李亚静,范吉平.槲皮素药理作用研究进展[J].辽宁中医药大学学报,2018,20(8):222-224.
    [8]
    Gholampour F,Saki N. Hepatic and renal protective effectsof quercetin in ferrous sulfate-induced toxicity[J].General Physiology and Biophysics,2019,38(1):27-38.
    [9]
    沈钦海,秦召敏,刘丽,等.槲皮素对肝细胞氧化应激损伤的保护作用[J].天津医药,2015,43(10):1102-1103.
    [10]
    汪静. 肝线粒体酒精性损伤中frataxin的介导效应与槲皮素保护机制[D].武汉:华中科技大学,2017.
    [11]
    刘姝. 急性酒精性肝损伤中槲皮素抗氧化应激及抗炎作用交互影响的机制研究[D].沈阳:中国医科大学,2018.
    [12]
    中华医学会肝病学分会脂肪肝和酒精性肝病学组,中国医师协会脂肪性肝病专家委员会.酒精性肝病防治指南(2018年更新版)[J].实用肝脏病杂志,2018,21(2):170-176.
    [13]
    Shuvendra S,Annayya A,Ricardo R,et al. In vivo acute on chronic ethanol effects in liver:A mouse model exhibiting exacerbated injury,altered metabolic and epigenetic responses[J].Biomolecules,2015,5(4):3280-3294.
    [14]
    Ki S H,Park O,Zheng M,et al. Interleukin-22 treatment ameliorates alcoholic liver injury in a murine model of chronic-binge ethanol feeding:role of signal transducer and activator of transcription 3[J]. Hepatology,2010,52(4):1291-1300.
    [15]
    Chinese Nutrition Society.The chinese dietary guidelines 2016[M].Beijing:People's Medical Publishing House,2016.
    [16]
    Zima T.Alcohol[J].Case Lek Cesk,1996,135:458-465.
    [17]
    Al-Asmari A K,Khan H A,Manthiri R A,et al. Protectiveeffects of a natural herbal compound quercetin against snakevenom-induced hepatic and renal toxicities in rats[J].Food and Chemical Toxicology,2018(118):105-110.
    [18]
    席彩彩,张文芳,侯明月,等.微波提取技术在中药有效成分提取中的应用[J].中国药业,2014,23(3):94-96.
    [19]
    Vallee B L,Hoch F L,Zinc A component of yeas talcoholdehydrogenase[J].Proceedings of the National Academy of Sciences of the United States of America,1955,41(6):327-338.
    [20]
    冯国基,崔巧生,冯爱荣.血清过氧化氢酶活活性比色测定法[J].现代诊断与治疗,1994(1):12-14.
    [21]
    吴霞明. 枸杞黄酮的研究进展[J]. 中国食物与营养,2018,24(8):9-13.
    [22]
    Volate S R,Davenport D M,Muga S J,et al. Modulation of aberrant crypt foci and apoptosis by dietary herbal supplements(quercetin,curcumin,silymarin,ginseng and rutin)[J]. Carcinogenesis,2005,26(8):1450-1456.
    [23]
    项昭保,伍晓玲,钟雪.响应面优化超声波辅助乙醇提取橄榄叶总黄酮的工艺研究[J].食品工业科技,2018,39(10):217-221.
    [24]
    Gu H Y,Chen F L,Zhang Q,et al. Application of ionic liquids in vacuum microwave-assisted extraction followed by macroporous resin isolation of three flavonoids rutin,hyperoside and hesperidin from Sorbus tianschanica leaves[J]. Journal of Chromatography B,2016,1014:45-55.
    [25]
    Zhou J,Zhang L,Li Q,et al. Simultaneous optimization forultrasound-assisted extraction and antioxidant activity offlavonoids from Sophora flavescens using response surfacemethodology[J].Molecules,2019,24(1):112.
    [26]
    翟广玉,朱玮,渠文涛,等.槲皮素的光谱分析[J].光谱实验室,2012,29(4):2443-2449.
    [27]
    戴雨霖. 葛花人参解酒护肝机制的研究[D].长春:长春中医药大学,2014.
    [28]
    吴晖,李丽丽,李晓凤,等.非水相中固定化酶水解制备玉米肽的体外醒酒活性研究[J].食品与发酵工业,2010,36(12):37-41.
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