Science and Technology of Food Industry

ISSN 1002-0306
CN   11-1759/TS
High-quality sci-tech periodicals
Menu
Volume 45 Issue 5
Feb.  2024
Turn off MathJax
Article Contents
Abstract
References
Supplements
ZHANG Qi, BAO Xiaobo, TIAN Chongchong. Effect of Scutellarin on Promoting the Sensitivity of Breast Cancer 4T1 Cells to Cisplatin in Vitro and in Vivo[J]. Science and Technology of Food Industry, 2024, 45(5): 331−340. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050219.
Citation: ZHANG Qi, BAO Xiaobo, TIAN Chongchong. Effect of Scutellarin on Promoting the Sensitivity of Breast Cancer 4T1 Cells to Cisplatin in Vitro and in Vivo[J]. Science and Technology of Food Industry, 2024, 45(5): 331−340. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023050219.
shu

Effect of Scutellarin on Promoting the Sensitivity of Breast Cancer 4T1 Cells to Cisplatin in Vitro and in Vivo

  • Department of Pharmacy, Jiangsu Vocational College of Medicine, Yancheng 224005, China

More Information
  • Received Date: May 17, 2023
  • Available Online: January 01, 2024
    Graphical Abstract
    • Abstract
  • To detect the effect of scutellarin (SCU) on promoting the sensitivity of breast cancer 4T1 cells to cisplatin (CDDP), and the molecular mechanism as well. CCK-8, scratch assay, Transwell assay and flow cytometry were employed to investigate the effects of SCU combined with CDDP on the proliferation, migration, invasion and apoptosis of 4T1 cells in vitro. Then, the 4T1 tumor-bearing mouse model was established to explore the effect of SCU combined with CDDP on tumor growth in vivo. The morphology, necrotic area and microvascular density of tumor tissues were observed by H&E staining. Real-time fluorescence quantitative PCR and Western Blot were used to detect the mRNA and proteins expression of apoptosis factors in tumor tissues. The results showed that SCU (200 μmol/L) combined with CDDP (80 μmol/L) significantly inhibited the in vitro proliferation of 4T1 cells (P<0.01). Moreover, the migration and invasion capacity of 4T1 cell were apparently reduced and the apoptosis of tumor cells were significantly promoted when treated with SCU (200 μmol/L) combined with CDDP (80 μmol/L) (P<0.01). Besides, the growth of 4T1 cells in vivo was remarkably slower after administration of 60 mg/kg SCU combined with 3.0 mg/kg CDDP (P<0.01). Further H&E semi-quantitative results revealed the microvascular density of 4T1 tumor tissues was significantly decreased (P<0.01). What’s more, the combination of SCU and CDDP significantly promoted the expression of pro-apoptotic factors Caspase-3, Bax, Caspase-9, Cleaved-Caspase-3 and Cleaved-Caspase-9 (P<0.01) and inhibited the expression of anti-apoptotic factor Bcl-2 in tumor tissues (P<0.05). In conclusion, SCU combined with CDDP could inhibit the function of tumor cells by enhancing the sensitivity of 4T1 cells to CDDP, and also regulate the expression of apoptosis factors, thus inhibiting the growth of tumor.
    • FullText(HTML)
    • References (31)
  • [1]
    SHIEN T, IWATA H. Adjuvant and neoadjuvant therapy for breast cancer[J]. Japanese Journal of Clinical Oncology,2020,50(3):225−229. doi: 10.1093/jjco/hyz213
    [2]
    LEE J S, YOST S E, YUAN Y. Neoadjuvant treatment for triple negative breast cancer:Recent progresses and challenges[J]. Cancers,2020,12(6):1404. doi: 10.3390/cancers12061404
    [3]
    于红, 张龙梅. 正元胶囊联合新辅助化疗治疗三阴性乳腺癌临床观察[J]. 中国中医药现代远程教育,2023,21(5):129−132. [YU H, ZHANG L M. Clinical observation on Zhengyuan capsule combined with neoadjuvant chemotherapy in the treatment of triple negative breast cancer[J]. Chinese Medicine Modern Distance Education of China,2023,21(5):129−132.] doi: 10.3969/j.issn.1672-2779.2023.05.048

    YU H, ZHANG L M. Clinical observation on Zhengyuan capsule combined with neoadjuvant chemotherapy in the treatment of triple negative breast cancer[J]. Chinese Medicine Modern Distance Education of China, 2023, 21（5）: 129−132. doi: 10.3969/j.issn.1672-2779.2023.05.048
    [4]
    BAI X, NI J, BERETOV J, et al. Triple-negative breast cancer therapeutic resistance:Where is the Achilles' heel?[J]. Cancer Letters,2021,497:100−111. doi: 10.1016/j.canlet.2020.10.016
    [5]
    ZHANG R, YANG Y, DONG W, et al. D-mannose facilitates immunotherapy and radiotherapy of triple-negative breast cancer via degradation of PD-L1[J]. Proceedings of the National Academy of Sciences,2022,119(8):e2114851119. doi: 10.1073/pnas.2114851119
    [6]
    WON K A, SPRUCK C. Triple-negative breast cancer therapy:Current and future perspectives[J]. International Journal of Oncology,2020,57(6):1245−1261. doi: 10.3892/ijo.2020.5135
    [7]
    YIN L, DUAN J J, BIAN X W, et al. Triple-negative breast cancer molecular subtyping and treatment progress[J]. Breast Cancer Research,2020,22(1):61. doi: 10.1186/s13058-020-01296-5
    [8]
    祁冶, 潘海婷, 王秀梅. SCR7对三阴性乳腺癌顺铂化疗的增敏作用研究[J]. 重庆医科大学学报,2023,48(2):220−225. [QI Y, PAN H T, WANG X M. Study on the sensitizing effect of SCR7 on cisplatin chemotherapy in triple-negative breast cancer[J]. Journal of Chongqing Medical University,2023,48(2):220−225.]

    QI Y, PAN H T, WANG X M. Study on the sensitizing effect of SCR7 on cisplatin chemotherapy in triple-negative breast cancer[J]. Journal of Chongqing Medical University, 2023, 48（2）: 220−225.
    [9]
    ECKSTEIN N. Platinum resistance in breast and ovarian cancer cell lines[J]. Journal of Experimental & Clinical Cancer Research,2011,30:1−11.
    [10]
    CARAMELO O, SILVA C, CARAMELO F, et al. The effect of neoadjuvant platinum-based chemotherapy in BRCA mutated triple negative breast cancers-systematic review and meta-analysis[J]. Hereditary Cancer in Clinical Practice,2019,17:1−10.
    [11]
    TORRISI R, ZURADELLI M, AGOSTINETTO E, et al. Platinum salts in the treatment of BRCA-associated breast cancer:A true targeted chemotherapy[J]. Critical Reviews in Oncology/Hematology,2019,135:66−75. doi: 10.1016/j.critrevonc.2019.01.016
    [12]
    REDDY S M, BARCENAS C H, SINHA A K, et al. Long-term survival outcomes of triple-receptor negative breast cancer survivors who are disease free at 5 years and relationship with low hormone receptor positivity[J]. British Journal of Cancer,2018,118(1):17−23. doi: 10.1038/bjc.2017.379
    [13]
    刘涛. 一种游戏茶饮及其制备方法:云南, CN107006648A[P]. 2017-08-04. [LIU T. A kind of tea drink and its preparation method:Yunnan, CN107006648A[P]. 2017-08-04.]

    LIU T. A kind of tea drink and its preparation method: Yunnan, CN107006648A[P]. 2017-08-04.
    [14]
    苟秀芹. 一种即食剁椒豆干:山东, CN201510999706.8[P]. 2016-04-13. [GOU X Q. A kind of instant dried tofu with chopped chili:Shandong, CN201510999706.8[P]. 2016-04-13.]

    GOU X Q. A kind of instant dried tofu with chopped chili: Shandong, CN201510999706.8[P]. 2016-04-13.
    [15]
    钱卫萍. 一种抑菌牙膏:江苏, CN201711204455.5[P]. 2018-02-09. [QIAN W P. A kind of bacteriostatic toothpaste:Jiangsu, CN201711204455.5[P]. 2018-02-09.]

    QIAN W P. A kind of bacteriostatic toothpaste: Jiangsu, CN201711204455.5[P]. 2018-02-09.
    [16]
    DAI J, LI C, ZHAO L, et al. Scutellarin protects the kidney from ischemia/reperfusion injury by targeting Nrf2[J]. Nephrology,2022,27(8):690−700. doi: 10.1111/nep.14069
    [17]
    PENG L, WEN L, SHI Q F, et al. Scutellarin ameliorates pulmonary fibrosis through inhibiting NF-κB/NLRP3-mediated epithelial–mesenchymal transition and inflammation[J]. Cell Death & Disease,2020,11(11):978.
    [18]
    ZENG S, CHEN L, SUN Q, et al. Scutellarin ameliorates colitis-associated colorectal cancer by suppressing Wnt/β-catenin signaling cascade[J]. European Journal of Pharmacology,2021,906:174253. doi: 10.1016/j.ejphar.2021.174253
    [19]
    ZHANG G Y, CHEN W Y, LI X B, et al. Scutellarin-induced A549 cell apoptosis depends on activation of the transforming growth factor-β1/smad2/ROS/caspase-3 pathway[J]. Open Life Sciences,2021,16(1):961−968. doi: 10.1515/biol-2021-0085
    [20]
    CHEN S, LI R, CHEN Y, et al. Scutellarin enhances anti-tumor immune responses by reducing TNFR2-expressing CD4+ Foxp3+ regulatory T cells[J]. Biomedicine & Pharmacotherapy,2022,151:113187.
    [21]
    JU S H, TAN L R, LIU P W, et al. Scutellarin regulates osteoarthritis in vitro by inhibiting the PI3K/AKT/mTOR signaling pathway[J]. Molecular Medicine Reports,2021,23(1):1.
    [22]
    田冲冲, 张琦, 包小波, 等. 灯盏花乙素对黑色素瘤B16体内外的作用及机制研究[J]. 食品工业科技,2023,44(17):406−412. [TIAN C C, ZHANG Q, BAO X B, et al. Effect of scutellarin on melanoma B16 in vitro and in vivo and its mechanism[J]. Science and Technology of Food Industry,2023,44(17):406−412.]

    TIAN C C, ZHANG Q, BAO X B, et al. Effect of scutellarin on melanoma B16 in vitro and in vivo and its mechanism[J]. Science and Technology of Food Industry, 2023, 44（17）: 406−412.
    [23]
    姚侠, 方杭, 金鑫. 灯盏乙素通过下调TRIM32的表达增强卡铂的抗卵巢癌活性研究[J]. 中国现代应用药学,2020,37(3):282−287. [YAO X, FANG H, JIN X. Scutellarin enhances carboplatin-induced cytotoxicity against ovarian cancer by decrease the expression of TRIM32[J]. Chinese Journal of Modern Applied Pharmacy,2020,37(3):282−287.]

    YAO X, FANG H, JIN X. Scutellarin enhances carboplatin-induced cytotoxicity against ovarian cancer by decrease the expression of TRIM32[J]. Chinese Journal of Modern Applied Pharmacy, 2020, 37（3）: 282−287.
    [24]
    GAO C, ZHOU Y, JIANG Z, et al. Cytotoxic and chemosensitization effects of scutellarin from traditional Chinese herb Scutellaria altissima L. in human prostate cancer cells[J]. Oncology Reports,2017,8(3):1491−1499.
    [25]
    CAO Y, FENG Y H, GAO L W, et al. Artemisinin enhances the anti-tumor immune response in 4T1 breast cancer cells in vitro and in vivo[J]. International Immunopharmacology,2019,70:110−116. doi: 10.1016/j.intimp.2019.01.041
    [26]
    张媛, 禹卓玥, 孙立新, 等. 卵巢癌组织MEOX1表达对细胞增殖和侵袭及迁移影响[J]. 中华肿瘤防治杂志,2020,27(4):262−268. [ZHANG Y, YU Z Y, SUN L X, et al. Expression of MEOX1 in ovarian cancer and its effect on the proliferation, invasion and migration[J]. Chinese Journal of Cancer Prevention and Treatment,2020,27(4):262−268.]

    ZHANG Y, YU Z Y, SUN L X, et al. Expression of MEOX1 in ovarian cancer and its effect on the proliferation, invasion and migration[J]. Chinese Journal of Cancer Prevention and Treatment, 2020, 27（4）: 262−268.
    [27]
    PULASKI B A, OSTRAND-ROSENBERG S. Mouse 4T1 breast tumor model[J]. Current Protocols in Immunology, 2000, 39(1):20.2. 1-20.2. 16.
    [28]
    CHANGIZI Z, MOSLEHI A, ROHANI A H, et al. Chlorogenic acid induces 4T1 breast cancer tumor's apoptosis via p53, Bax, Bcl-2, and caspase-3 signaling pathways in BALB/c mice[J]. Journal of Biochemical and Molecular Toxicology,2021,35(2):e22642. doi: 10.1002/jbt.22642
    [29]
    DA SILVA LAWISCH G K, BIOLCHI V, et al. The role of FASL, BCL-2 and BAX polymorphisms in Brazilian patients with prostate cancer and benign prostatic hyperplasia[J]. Molecular Biology Reports,2022,49(10):9445−9451. doi: 10.1007/s11033-022-07805-3
    [30]
    WANG H W, ZHAO W P, LIU J, et al. Fluoride-induced oxidative stress and apoptosis are involved in the reducing of oocytes development potential in mice[J]. Chemosphere,2017,186:911−918. doi: 10.1016/j.chemosphere.2017.08.068
    [31]
    WANG S, ZHANG Y, GAO J, et al. The enantioselective study of the toxicity effects of chiral acetochlor in HepG2 cells[J]. Ecotoxicology and Environmental Safety,2021,218:112261. doi: 10.1016/j.ecoenv.2021.112261
    • Related Articles
  • Supplements (1)

  • Other Related Supplements

  • Cited by

    Periodical cited type(15)

    1. 郭松，肖善芳，江凯欣，梁湘兰. 暖骨风多酚的提取工艺优化及其抗氧化和抑菌性的初步研究. 饲料工业. 2024(04): 113-120 .
    2. 安小雯，李帅，王玲玲，孙沛，吴澎. 我国药食同源物质保健食品的发展现状及对策研究. 中国果菜. 2024(05): 15-19+72 .
    3. 罗维巍，吕琳琳，孙丽阳，李铁纯，郭俊生，刁全平. 黑涩石楠总多酚超声辅助提取工艺优化、抗氧化活性评价及单体酚分析. 中国酿造. 2024(05): 199-205 .
    4. 侯文翼，段振华，易弋. 微波干燥对月柿果片不同区域酚类含量及色泽的影响. 现代食品科技. 2024(10): 259-269 .
    5. 刘庆双，杨晓宽. 一种新型板栗醋多酚的提取纯化及其减肥降脂作用. 食品与发酵工业. 2024(24): 212-220 .
    6. 李琼. 沙棘复合果汁饮料研制及HACCP质量控制研究. 食品工业. 2023(02): 41-45 .
    7. 刘勇，张彩虹，谢普军，邓叶俊，黄立新. 皂角刺化学组成及药理活性应用研究进展. 生物质化学工程. 2023(02): 89-98 .
    8. 刘桢，吕玉秀，张璟雯，谢东，王琪，雷悦，赵平，朱绍志，杨晓琴. 雀嘴茶中三大酚类成分的抗氧化活性和酪氨酸酶抑制活性分析. 食品工业科技. 2023(08): 405-411 . 本站查看
    9. 华玮，熊丽娜，张永鑫，孙鹏，岳龙，付鸿博. 草莓多酚提取工艺优化及抗氧化性分析. 果树资源学报. 2023(06): 17-22 .
    10. 李晓静，魏晓博，程雪，张志刚，刘洪涛，刘慧燕，方海田. 人肠道乳酸片球菌的分离鉴定及其发酵枸杞汁性能分析. 食品工业科技. 2023(22): 143-151 . 本站查看
    11. 李爽，韩海霞，沙比拉·祖农买买提，马瑞，高娟娟. 姜草枣复合膏的制备及其贮藏稳定性. 食品研究与开发. 2023(22): 108-115 .
    12. 代琪，白苑丁，叶俏波，杨小艳，赵小勤，王欣. 不同产地大枣化学成分及其药理作用研究进展. 中国药物评价. 2023(06): 506-511 .
    13. 李静，葛世浩，多佳，严欢，马梦亚，刘军. 短时黑蒜体外抗氧化活性及其多酚提取优化. 食品工业. 2022(11): 117-123 .
    14. 罗宇鑫，金灵，马慧敏，向延菊. 阿拉尔地区不同品种红枣品质分析. 食品工业. 2022(11): 331-335 .
    15. 张婷飞，孟秀梅，张兰，曹阳，田其英，李琴，张丽芳. 槐米红枣面包的研制. 现代食品. 2022(20): 69-73 .

    Other cited types(14)

Catalog

    Get Citation
    PDF
    XML

    Article Metrics

    Article views (90) PDF downloads (18) Cited by(29)
    Address：No. 70, Shazikou Road, Yongding gate, BeijingPostcode：100101
    Tel：010-87244116 87244117Fax :010-87287944
    RSS Email Alert

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return