稀有人参皂苷compound K转化菌株的筛选及转化条件优化

doi:10.13386/j.issn1002-0306.2013.11.020

稀有人参皂苷compound K转化菌株的筛选及转化条件优化

基金项目:

吉林省自然科学基金项目(200905106)；

详细信息

中图分类号: TQ920.6
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出版历程
- 收稿日期: 2012-12-24

Screening of phytopathogenic fungi by minor ginsenoside compound K production and optimization of biotransformation conditions

摘要

摘要: 从22种植物病原真菌中筛选出6种能够转化原人参二醇型皂苷为稀有人参皂苷compound K（C-K）的真菌,其中转化效率最高的1.91号真菌经形态学鉴定为卵形孢霉属（Oospora Wallr.）真菌。1.91号真菌生物转化人参皂苷Rb1和Rb2的途径分别为:Rb1→Rd→F2→C-K,Rb2→C-O→C-Y。1.91号真菌生物转化人参皂苷Rb1为C-K的最佳条件为:最佳底物添加时间是孢子预培养12h后,转化最适pH5.0～6.0,转化最适温度是45℃。本研究为稀有人参皂苷C-K的工业制备奠定了一定的基础。
- 人参皂苷; /
- compound K; /
- 生物转化; /
- 筛选;
Abstract: Twenty-two phytopathogenic fungi were screened with regard to their ability to produce minor ginsenoside compound K (C-K) . Six fungi were detected to be able to convert major protopanaxadiol type saponins to ginsenoside C-K in the all tested strains, and the No. 1. 91 fungal strain which showed the most efficient biotransformation was morphologically identified as Oospora Wallr. The biotransformation pathways of ginsenoside Rb1 and Rb2 by No. 1. 91 fungal strain were:Rb1→Rd→F2→C-K, Rb2→C-O→C-Y. The following optimum conditions for transforming C-K by No. 1. 91 fungal strain were determined:the time of substrate addition, 12h, pH5. 0 ～ 6. 0 and temperature 45℃. This study laid a certain foundation of the industrial preparation of minor ginsenoside C-K.
- ginsenosides;compound K;biotransformation;screening; /
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参考文献(15)

[1]	张伟云, 陈全成, 郑毅男, 等.RP-HPLC测定人参各部位人参皂苷compound K的含量[J].药物分析杂志, 2007, 27:1-3.
[2]	Bae EA, Kim NY, Han MJ, et al.Transformation of ginsenosides to compound K (1H-901) by lactic acid bacteria of human intestine[J].J Microbiol Biotechn, 2003, 13 (1) :9-14.
[3]	Suda K, Murakami K.Induction of apoptosis in Lewis lung carcinoma cells by an intestinal bacterial metabolite produced from orally administered ginseng protopanaxadiol saponins[J].J Trad Med, 2000, 17:236-244.
[4]	Kim S, Kang BY, Cho SY, et al.Compound K induces expression of hyaluronan synthase2gene in transformed human keratin℃ytes and increases hyaluronan in hairless mouse skin[J].Bi℃hem Biophys Res Commun, 2004, 316:348-355.
[5]	Park EK, Shin YW, Lee HU, et al.Inhibitory effect of ginsenoside Rb land compound K on NO and prostaglandin E2biosyntheses of RAW264.7cells induced by lipopolysaccharide[J].Biol Pharm Bull, 2005, 28 (4) :652-656.
[6]	Shin YW, Bae EA, Kim SS, et al.Effect of ginsenoside Rb land compound K in chronic oxazolone-induced mouse dermatitis[J].Int Immunopharmacol, 2005, 5:1183-1191.
[7]	李伟.人参皂苷Compound K对2型糖尿病的降血糖作用及肝糖异生信号转导通路调控[D].长春:吉林大学, 2012.
[8]	Lee HU, Bae EA, Han MJ, et al.Hepatoprotective effect of ginsenoside Rb land compound K on tert-butyl hydroperoxide-induced liver injury[J].Liver Int, 2005, 25:1069-1073.
[9]	李学, 臧埔, 张连学, 等.微生物转化法制备人参皂苷Compound K的研究进展[J].食品科学, 2012, 33 (11) :323-327.
[10]	杨元超, 王英平, 闫梅霞, 等.人参皂苷compound K转化菌株的筛选[J].中国中药杂志, 2011, 36 (12) :1596-1598.
[11]	宋成程, 赵雪淞, 杜秀丽, 等.毁灭柱孢菌中一种人参皂苷Rb1水解酶的纯化及酶学性质研究[J].微生物学杂志, 2011, 31 (5) :20-26。
[12]	Yan Q, Zhou W, Shi XL, et al.Biotransformation pathways of ginsenoside Rb1to compound K byβ-glucosidases in fungus Paecilomyces Bainier sp.229[J].Process Biochem, 2010, 45:1550-1556.
[13]	Noh KH, Son JW, Kim HJ, et al.Ginsenoside Compound K production from ginseng root extract by a thermostableβ-glycosidase from Sulfolobus solfataricus[J].Biosci Biotechnol Biochem, 2009, 73:316-321.
[14]	Juan Gao, Wenjing Xu, Qiang Fang, et al.Efficient biotransformation for preparation of pharmaceutically activeginsenoside Compound K by Penicillium oxalicum sp.68[J].Ann Microbiol, 2012, 4, DOI10.1007/s13213-012-0454-3.
[15]	魏景超.真菌鉴定手册[M].上海:上海科学技术出版社, 1979:129-135.