Abstract: This paper aimed to investigate the antioxidant activity of buckwheat bee pollen polysaccharides (BWPP) during the in vitro gastrointestinal digestion. The crude polysaccharides were purified using DEAE-52 cellulose column and their antioxidant activity during in vitro simulated gastric and intestinal digestion was studied. The results showed that after purification by DEAE-52 cellulose column chromatography, the highest total sugar content component BWPP-1 was obtained, which was mainly composed of Glc, Xyl, Ara, etc. The infrared spectrum showed that BWPP-1 had obvious polysaccharide characteristic absorption peaks. During the simulated in vitro gastrointestinal digestion, the ABTS⁺· scavenging rates of BWPP and BWPP-1 reached their maximum values at 60 minutes of simulated gastric juice digestion, which were 86.39%±1.28% and 90.38%±2.78%, respectively. There was a significant difference compared to other digestion times (P<0.05). The DPPH· clearance rates reached their maximum values at 60 minutes, which were 86.86%±0.11% and 89.58%±1.67%, respectively, and the difference between the two was not significant (P>0.05). When simulating intestinal digestion, the maximum clearance rates of ABTS⁺· by BWPP and BWPP-1 reached 88.67%±0.40% and 91.82%±2.77% at 60 minutes, and the difference between the two was not significant (P>0.05). The DPPH· scavenging rates reached their peak values at 240 minutes of 57.11%±0.06% and 65.67%±3.67%, with significant difference (P<0.05). During the digestion process, the ability to reduce iron ions was relatively weak. Based on these findings, it can be concluded that BWPP and BWPP-1 have good ABTS⁺· and DPPH· scavenging abilities, and the DPPH· scavenging ability during gastric digestion is slightly higher than that during intestinal digestion, while the ABTS⁺· scavenging ability during intestinal digestion is slightly higher than that during gastric digestion. This study provides a theoretical basis for the development of functional products based on buckwheat bee pollen.