Abstract: Isomaltooligosaccharides (IMOs) are recognized as functional food ingredients with prebiotic potential that deliver health benefits. IMOs have attained commercial interest as they are produced from low-cost agricultural products that are widely available and have prospective applications in the food industry. This paper focuses on the enzymatic production processes of IMOs, as well as its key technology research, including enzyme conversion, enzyme membrane bioreactors, enzyme immobilization and biocatalysis are analyzed. Moreover, the shortcomings of the existing technology are analyzed. Combined with modern biotechnology, the research direction of related enzyme technology in the field of industrial production of IMOs is prospected, which provide some reference for its industrialization development.