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中国精品科技期刊2020
庄燕苹,杨帆,肖曼,等. 忧遁草乙醇提取物对秀丽隐杆线虫的抗衰老作用及机制[J]. 食品工业科技,2023,44(11):411−417. doi: 10.13386/j.issn1002-0306.2022090024.
引用本文: 庄燕苹,杨帆,肖曼,等. 忧遁草乙醇提取物对秀丽隐杆线虫的抗衰老作用及机制[J]. 食品工业科技,2023,44(11):411−417. doi: 10.13386/j.issn1002-0306.2022090024.
ZHUANG Yanping, YANG Fan, XIAO Man, et al. Anti-aging Effect and Mechanism of Ethanol Extract of Clinacanthus nutans on Caenorhabditis elegans[J]. Science and Technology of Food Industry, 2023, 44(11): 411−417. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090024.
Citation: ZHUANG Yanping, YANG Fan, XIAO Man, et al. Anti-aging Effect and Mechanism of Ethanol Extract of Clinacanthus nutans on Caenorhabditis elegans[J]. Science and Technology of Food Industry, 2023, 44(11): 411−417. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022090024.

忧遁草乙醇提取物对秀丽隐杆线虫的抗衰老作用及机制

Anti-aging Effect and Mechanism of Ethanol Extract of Clinacanthus nutans on Caenorhabditis elegans

  • 摘要: 目的:以秀丽隐杆线虫为模式生物,探究忧遁草乙醇提取物(Clinacanthus nutans ethanol extract,CNEE)对线虫的抗衰老作用及作用机制。方法:通过寿命、产卵、运动等实验检测忧遁草乙醇提取物对线虫抗衰老的作用;通过急性氧化应激、抗氧化酶活力测定、活性氧(ROS)测定、实时荧光定量PCR等实验初步探究忧遁草乙醇提取物的抗氧化能力及延缓衰老的作用机制。结果:忧遁草乙醇提取物在100 μg/mL时能延长N2野生型线虫寿命(P<0.001),对其运动能力也有促进作用(P<0.001),同时对产卵能力没有毒性影响(P>0.05);该提取物还可以提高线虫在急性氧化应激条件下的寿命(P<0.05)及过氧化氢酶的活力(P<0.01),并有效降低体内ROS的堆积情况(P<0.05),上调daf-16P<0.01)及下游ctl-1P<0.001)基因的表达水平。但在daf-16突变株线虫中,没有观察到正常条件或急性氧化应激条件下延寿的作用,也没有提高过氧化氢酶的活力、降低ROS水平、提高daf-16ctl-1表达水平的情况(P>0.05)。结论:忧遁草乙醇提取物可以提高秀丽隐杆线虫抗衰老的能力,其作用机制可能与daf-16的氧化应激调控有关。

     

    Abstract: Objective: To assess the anti-aging effect and mechanism underlying the effects of ethanol extract from Clinacanthus nutans (CNEE) using Caenorhabditis elegans as the model organism. Methods: The anti-aging effects of ethanol extract from Clinacanthus nutans on nematodes were detected by experiments to assay longevity, oviposition, and exercise. The antioxidant capacity of the ethanolic extracts from Clinacanthus nutans and their mechanisms of action in delaying aging were investigated by assessing the acute oxidative stress, determining the antioxidant enzyme activity, and determining the reactive oxygen species (ROS) levels along with real-time fluorescence quantitative PCR experiments. Results: The ethanol extract from Clinacanthus nutans at 100 μg/mL could prolong the life span of N2 wild type nematode (P<0.001), promote the ability to exercise (P<0.001), and exerted no toxic effects on oviposition (P>0.05). The extract also increased nematode longevity (P<0.05) and catalase activity (P<0.01) under acute oxidative stress conditions, and effectively reduced the accumulation of ROS in vivo (P<0.05) along with upregulating the levels of daf-16 (P<0.01) and downstream ctl-1 (P<0.001) gene expressions. However, in daf-16 mutant nematodes, effects on life extension under normal conditions or acute oxidative stress were not observed, nor was there an increase in catalase activity, a decrease in ROS levels, or an increase in daf-16 and ctl-1 levels (P>0.05). Conclusion: The ethanol extract from Clinacanthus nutans enhanced the ability of nematodes to resist aging and the underlying mechanism of action may be related to the regulation of oxidative stress by daf-16.

     

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