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中国精品科技期刊2020
丁海臻,刘纪伟,常乐,等. 基于FT-NIR光谱技术快速定量分析米糠中结合态与游离态酚类含量[J]. 食品工业科技,2023,44(8):326−333. doi: 10.13386/j.issn1002-0306.2022070026.
引用本文: 丁海臻,刘纪伟,常乐,等. 基于FT-NIR光谱技术快速定量分析米糠中结合态与游离态酚类含量[J]. 食品工业科技,2023,44(8):326−333. doi: 10.13386/j.issn1002-0306.2022070026.
DING Haizhen, LIU Jiwei, CHANG Le, et al. Rapid Quantitative Detection of Bound and Free Phenolic Contents in Rice Bran by Using Fourier Transform Near Infrared Spectroscopy[J]. Science and Technology of Food Industry, 2023, 44(8): 326−333. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070026.
Citation: DING Haizhen, LIU Jiwei, CHANG Le, et al. Rapid Quantitative Detection of Bound and Free Phenolic Contents in Rice Bran by Using Fourier Transform Near Infrared Spectroscopy[J]. Science and Technology of Food Industry, 2023, 44(8): 326−333. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022070026.

基于FT-NIR光谱技术快速定量分析米糠中结合态与游离态酚类含量

Rapid Quantitative Detection of Bound and Free Phenolic Contents in Rice Bran by Using Fourier Transform Near Infrared Spectroscopy

  • 摘要: 针对新鲜米糠酚类含量检测的时效性不足的问题,本文建立了一种基于傅里叶转化近红外光谱技术(FT-NIR)的米糠酚类组分快速无损检测方法。以多批次的新鲜米糠作为实验原料,定量分析了其游离态酚类、结合态酚类以及总酚含量,构建了基于全波段和特征波段的偏最小二乘回归(PLSR)、支持向量机(SVM)、BP人工神经网络(BPNN)的预测模型。结果表明:在全波段数据建模中,基于PLSR模型的预测结果(结合态、游离态以及总酚)相对最佳,对应的R_\rmp^2 为0.944、0.943和0.937,RPD为3.031、2.779和2.863;采用竞争适应性重加权采样法(CARS)和连续投影算法(SPA)分别提取了4~8个特征波段,其中基于CARS-PLSR(结合态、游离态以及总酚)预测效果相对最佳,对应的R_\rmp^2 为0.953、0.932和0.944,RPD为3.301、2.759和3.031,建模的运行时间缩短2倍,仅需2 s,符合米糠中酚类物质检测的时效性需求。本研究结果证实了基于FT-NIR技术可以实现米糠中酚类含量组分的快速定量检测。

     

    Abstract: In order to solve the insufficient timeliness in the detection of phenolic contents in fresh rice bran, a rapid nondestructive detection method by using Fourier transform near infrared spectroscopy (FT-NIR) was constructed in this work. Multiple batches of fresh rice bran were utilized as experimental samples. Contents of free, bound and total phenolic compounds in fresh rice bran were quantitatively detected, and the prediction models of partial least squares regression (PLSR), support vector machine (SVM) and back propagation neural network (BPNN) were established based on full and characteristic wavelengths of FT-NIR. The results showed that the models based on PLSR algorithm obtained the best predicted performance based on full-wavelength datasets, the R_\rmp^2 reached 0.944, 0.943 and 0.937, and RPD reached 3.031, 2.779 and 2.863 for free, bound and total phenolic compounds, respectively. Two variable selection methods, named competitive adaptive reweighted sampling (CARS) and continuous projection (SPA) algorithms, were used in this work, and several key wavelengths from 4 to 8 numbers were selected. Best predicted models using key-wavelength datasets were constructed by CARS-PLSR, with R_\rmp^2 of 0.953, 0.932 and 0.944 for bound, free and total contents, respectively. The RPD could obtain with 3.301, 2.759 and 3.031, respectively. Meanwhile, the running time of modeling was shortened by 2 times and only needed 2.0 s, which could meet the timeliness requirement of detection of phenolic compounds in rice bran. The results of this study confirmed that FT-NIR technique could be used for rapid and quantitative determination of phenolic components in rice bran.

     

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