Abstract:
The effects of different inoculation levels (10
5, 10
6 and 10
7 CFU/g) of
Lactobacillus sakei on the quality characteristics of low-sodium dry sausages were investigated in this study. The uninoculated dry sausage was the control, and the moisture content, water activity, pH, lactic acid bacteria count, color and shear force were measured on days 0, 3, 6 and 9, respectively. Meanwhile, the flavor characteristics of the dry sausages on day 9 were analyzed by combination of gas chromatography-mass spectrometry and electronic nose. The results showed that pH of dry sausages inoculated with 10
6 and 10
7 CFU/g
L. sakei significantly decreased at the end of fermentation (
P<0.05), and the inoculation of 10
6 CFU/g could promote the improvement of moisture content and water activity of final product compared with the control. In addition, the color and shear force of all dry sausages did not change significantly with the inoculation levels (
P>0.05). The inoculation of
L. sakei reduced the contents of aldehydes, and only the inoculation of 10
6 CFU/g increased the contents of alcohols, acids and esters (
P<0.05), which caused a stronger characteristic odor than the control. Sensory evaluation results showed that inoculation of 10
6 CFU/g had the best effect on improving the bitterness and metallic taste, which resulted in the best overall acceptability of low-sodium dry sausages. Partial least squares regression analysis of physicochemical and sensory characteristics of dry sausages were consistent with the above results. The inoculation of 10
6 CFU/g had a positive effect on improving quality and developing good flavor of dry sausages.