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中国精品科技期刊2020
徐伟,王植朔,王瑞琦,等. 榛蘑与菌丝体中蜜环菌素的超声波辅助提取条件优化及其提取物中化合物分析[J]. 食品工业科技,2022,43(19):298−306. doi: 10.13386/j.issn1002-0306.2022050143.
引用本文: 徐伟,王植朔,王瑞琦,等. 榛蘑与菌丝体中蜜环菌素的超声波辅助提取条件优化及其提取物中化合物分析[J]. 食品工业科技,2022,43(19):298−306. doi: 10.13386/j.issn1002-0306.2022050143.
XU Wei, WANG Zhishuo, WANG Ruiqi, et al. Optimization of Ultrasonic-assisted Extraction Conditions of Melleolides from Wild Armillaria mellea and Liquid Culture Mycelium, and Analysis of Their Compounds in Extracts[J]. Science and Technology of Food Industry, 2022, 43(19): 298−306. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050143.
Citation: XU Wei, WANG Zhishuo, WANG Ruiqi, et al. Optimization of Ultrasonic-assisted Extraction Conditions of Melleolides from Wild Armillaria mellea and Liquid Culture Mycelium, and Analysis of Their Compounds in Extracts[J]. Science and Technology of Food Industry, 2022, 43(19): 298−306. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022050143.

榛蘑与菌丝体中蜜环菌素的超声波辅助提取条件优化及其提取物中化合物分析

Optimization of Ultrasonic-assisted Extraction Conditions of Melleolides from Wild Armillaria mellea and Liquid Culture Mycelium, and Analysis of Their Compounds in Extracts

  • 摘要: 目的:为分析榛蘑与菌丝体中化合物差异,本研究以东北野生榛蘑子实体和液态菌丝体为研究对象,探究蜜环菌素的最佳提取工艺条件,并对提取物中化合物进行分析。方法:以提取物得率和蜜环菌素含量为指标,采用超声波细胞破碎辅助石油醚进行萃取,通过单因素实验和正交试验对提取工艺参数进行优化;采用超高效液相色谱-串联质谱(UHPLC-MS/MS)技术,对液态菌丝体和榛蘑子实体中化合物进行分析和鉴定。结果:确定最佳提取工艺条件为料液比1:20 g/mL,超声波功率300 W,超声时间20 min,溶剂回流时间50 min,在该条件下液态菌丝体提取物得率为26.8%,蜜环菌素含量为0.74 mg/g;液态菌丝体和榛蘑子实体中化合物分别为305和592个。解析出16种蜜环菌素类和5种疑似蜜环菌素碎片物质,其中菌丝体中含有15种蜜环菌素和3种碎片,总含量分别为2.551和0.588 µg/mL;子实体中含有7种蜜环菌素和4种碎片,总含量分别为2.413和2.124 µg/mL。两者同有组分有6种蜜环菌素和2种碎片;液态菌丝体独有9种蜜环菌素和1种碎片为:10-dehydroxymelleoloede、蜜环菌辛素、Arnamiol、蜜环菌壬素、A52a、4-dehydroxyarmillarin、蜜环菌丙素、(4R,5S,7R,9S,13R)-2’,5-epoxy-4-dehydroxyarmillarin、4’-demethoxyarmillaribin以及碎片C;榛蘑子实体独有1种蜜环菌素和2种碎片为:Melledonal以及碎片D和E。结论:液态菌丝体蜜环菌素类化合物多于子实体中,为开发辅助功能食品及药物提供参考。

     

    Abstract: Objective: To analyze the differences of compounds in Armillaria mellea and mycelium, this study was conducted to investigate the optimal extraction process conditions of Melleolides and analyze the compounds in the extracts using northeastern wild Armillaria mellea fruit body and liquid mycelium as the research objects. Methods: The indexes of this study were extracted yield and Melleolides content. Ultrasonic cell crushing-assisted petroleum ether was used for extraction, and the extraction process parameters were optimized by single-factor experiments and orthogonal tests. The compounds in the liquid mycelium and Armillaria mellea fruit body were analyzed and identified by ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Results: The optimal extraction process conditions were determined as material-liquid ratio 1:20 g/mL, ultrasonic power 300 W, ultrasonic time 20 min, solvent reflux time 50 min, under which the yield of liquid culture mycelium extract was 26.8% and the content of Melleolides was 0.74 mg/g. 305 and 592 compounds were analyzed and identified in the liquid mycelium and wild Armillaria mellea, respectively. 16 Melleolides and 5 fragments of suspected Melleolides were identified, including 15 Melleolides and 3 fragments in the mycelium, with total contents of 2.551 and 0.588 µg/mL, respectively. 7 Melleolides and 4 fragments in fruit body, with total contents of 2.413 and 2.124 µg/mL, respectively. 6 Melleolides and 2 fragments were found in both; The liquid mycelium had 9 unique Melleolides and 1 fragment: 10-dehydroxymelleoloede, Nectarine, Arnamiol, Bexagliflozin, A52a, 4-dehydroxyarmillarin, 9-(2-Chloro-4-hydroxy-5-methoxyphenyl)-3,3,6,6-tetramethyl-3,4,5,6,7,9-hexahydro-1H-xanthene-1,8(2H)-dione, (4R,5S,7R,9S,13R)-2',5-epoxy-4-dehydroxyarmillarin, 4'-demethoxyarmillaribin, and fragment C. Wild Armillaria mellea uniquely possessed one Melleolides and two fragments as: Melledonal as well as fragments D and E. Conclusion: The results showed that Melleolides were more abundant in the liquid mycelium than in the fruit body, providing reference for the development of complementary functional foods and drugs.

     

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