Abstract: In this work, Pinus masoniana and Saxifraga stolonifera (L.) Meerb. essential oils were employed to develop preservative materials (i.e., preservative paper and preservative tablet), and effects of the preservative material treatments on Shine Muscat grapes under different temperatures (room temperature and low temperature) were also investigated. The results showed that the preservative paper was more effective than the preservative tablet to maintain the quality of the Shine Muscat in storage time. In addition, grapes treated with the preservative paper under low-temperature storage exhibited the optimum quality among all. Pinus masoniana-Saxifraga stolonifera (L.) Meerb. essential oils preservative paper effectively inhibited the weight loss rate, maintained the color and hardness, slowed down the decrease of total soluble solids, acidity and reducing sugar. After storage for 5 weeks under low temperature with the preservative paper, the weight loss rate of the grapes was 0.36%, significantly lower than that of the control group (0.81%, P<0.05), the total soluble solids and acidity were 1.65% and 0.13% significantly higher than those of the control group (P<0.05), respectively. In addition, the increase of the activities of polyphenol oxidase, peroxidase and malondialdehyde content in grapes were effectively inhibited by the preservative paper, the malondialdehyde content was 0.88 nmol/g, significantly lower than that in the control group (3.25 nmol/g, P<0.05). In conclusion, Pinus masoniana and Saxifraga stolonifera (L.) Meerb. essential oils preservative paper plays the role of maintaining the quality of the Shine Muscat during storage. Besides, preservative paper is convenient to use and has the prospect of industrialization.