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中国精品科技期刊2020

冷藏鲣鱼优势腐败产胺菌分离鉴定及其致腐产胺能力分析

刘洋帆 王迪 李春生 相欢 陈胜军 杨贤庆 李来好 邓建朝

刘洋帆,王迪,李春生,等. 冷藏鲣鱼优势腐败产胺菌分离鉴定及其致腐产胺能力分析[J]. 食品工业科技,2022,43(23):168−175. doi:  10.13386/j.issn1002-0306.2022040070
引用本文: 刘洋帆,王迪,李春生,等. 冷藏鲣鱼优势腐败产胺菌分离鉴定及其致腐产胺能力分析[J]. 食品工业科技,2022,43(23):168−175. doi:  10.13386/j.issn1002-0306.2022040070
LIU Yangfan, WANG Di, LI Chunsheng, et al. Isolation and Identification of Dominant Spoilage Biogenic Amine Producing Bacteria from Refrigerated Skipjack Tuna and Analysis of Their Production Ability[J]. Science and Technology of Food Industry, 2022, 43(23): 168−175. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022040070
Citation: LIU Yangfan, WANG Di, LI Chunsheng, et al. Isolation and Identification of Dominant Spoilage Biogenic Amine Producing Bacteria from Refrigerated Skipjack Tuna and Analysis of Their Production Ability[J]. Science and Technology of Food Industry, 2022, 43(23): 168−175. (in Chinese with English abstract). doi:  10.13386/j.issn1002-0306.2022040070

冷藏鲣鱼优势腐败产胺菌分离鉴定及其致腐产胺能力分析

doi: 10.13386/j.issn1002-0306.2022040070
基金项目: 广东省重点领域研发计划(2021B0202060001, 2019B020225001);财政部和农业农村部财政专项(NFZX2021);国家现代农业产业技术体系(CARS-47);广东省现代农业产业技术体系创新团队建设专项(2022KJ151);中国水产科学研究院基本科研业务费资助(2020TD73)。
详细信息
    作者简介:

    刘洋帆(1998−),女,硕士研究生,研究方向:水产品加工与质量安全,E-mail:18742026042@163.com

    通讯作者:

    邓建朝(1977−),男,博士,副研究员,研究方向:水产品加工与质量安全,E-mail:djc9801@foxmail.com

  • 中图分类号: TS201.3

Isolation and Identification of Dominant Spoilage Biogenic Amine Producing Bacteria from Refrigerated Skipjack Tuna and Analysis of Their Production Ability

  • 摘要: 为探究鲣鱼中优势腐败产胺菌的种类,从冷藏的鲣鱼中筛选出3种优势腐败产胺菌,通过16S rDNA分子鉴定技术对菌株进行鉴定为荧光假单胞菌(Pseudomonas fluorescens)、弗氏柠檬酸杆菌(Citrobacter freundii)和嗜水气单胞菌(Aeromonas hydrophila),将鉴定出的优势腐败产胺菌接种至无菌鱼肉中4 ℃条件下贮藏,通过测定菌落总数和挥发性盐基氮(TVB-N)变化,以腐败代谢产物产量因子(YTVB-N/CFU)分析3种优势腐败产胺菌对鲣鱼的致腐能力,并通过样品中生物胺的含量比较3种优势腐败产胺菌的产胺能力。结果表明:在贮藏8 d后接种荧光假单胞菌、弗氏柠檬酸杆菌和嗜水气单胞菌组的菌落总数分别达到8.36、8.27和8.13 lg CFU/g,TVB-N值分别为28.21、30.30和31.29 mg/100 g,3种优势腐败产胺菌对鲣鱼的致腐能力大小为嗜水气单胞菌>弗氏柠檬酸杆菌>荧光假单胞菌。接种荧光假单胞菌组、弗式柠檬酸杆菌组和嗜水气单胞菌组的组胺含量分别为196.23、83.43和261.22 mg/kg,3组样品中总生物胺含量为嗜水气单胞菌>荧光假单胞菌>弗氏柠檬酸杆菌。综合比较得出,嗜水气单胞菌对4 ℃冷藏鲣鱼致腐产胺能力最强,本研究增加对冷藏鲣鱼中优势腐败产胺菌种类的了解并提供了部分理论基础。
  • 图  1  8种菌株的16S rDNA电泳图谱

    Figure  1.  16S rDNA electrophoresis map of 8 strains

    图  2  3种菌株16S rDNA序列的系统发育树

    Figure  2.  Phylogenetic tree of 16S rDNA sequences from 3 strains

    注:A为T4的系统发育树;B为T6的系统发育树;C为T7的系统发育树。

    图  3  鲣鱼接种3种优势腐败产胺菌的菌落总数变化

    Figure  3.  Changes in colony counts of 3 dominant spoilage biogenic amine-producing bacteria inoculated to skipjack tuna

    图  4  鲣鱼接种3种优势腐败产胺菌的TVB-N值变化

    Figure  4.  Changes in TVB-N values of 3 dominant spoilage biogenic amine-producing bacteria inoculated to skipjack tuna

    图  5  贮藏第8天4组样品的总生物胺含量

    Figure  5.  Total biogenic amine content of the four groups of samples on the 8th day storage

    注:不同小写字母表示组别之间存在显著性差异(P<0.05)。

    表  1  鲣鱼接种3种优势腐败产胺菌致腐因子的比较

    Table  1.   Comparison of yield factors in skipjack tuna inoculated with 3 dominant spoilage biogenic amine-producing bacteria

    菌名菌落总数(CFU/g)腐败代谢产物含量(mg N/100 g)腐败代谢产物产量因子(mg/CFU)
    N0Ni(TVB-N)0(TVB-N)iYTVB-N/CFU
    荧光假单胞菌5.57×1052.60×1086.7128.218.39×10−8
    弗氏柠檬酸杆菌3.87×1052.00×1086.2230.301.20×10−7
    嗜水气单胞菌5.63×1051.7×1086.6731.291.48×10−7
    下载: 导出CSV

    表  2  鲣鱼接种3种优势腐败产胺菌的生物胺含量

    Table  2.   Biogenic amines content of 3 dominant spoilage biogenic amine-producing bacteria inoculated to skipjack tuna

    菌株时间(d)生物胺含量 (mg/kg)
    色胺酪胺苯乙胺组胺尸胺腐胺
    空白对照0NDND0.28±0.02d0.67±0.01a1.95±0.15aND
    1NDND0.23±0.00c0.88±0.00a2.44±0.16bND
    2NDND0.24±0.00c0.86±0.00a3.16±0.17cND
    3NDND0.19±0.00c1.57±0.01b5.11±0.27fND
    4NDND0.17±0.01a1.79±0.06bc5.49±0.04fND
    5NDND0.18±0.02ab1.99±0.23c4.65±0.02eND
    6NDND0.17±0.00a2.13±0.26d4.43±0.29eND
    7ND0.43±0.34a0.23±0.00c3.35±0.10e3.52±0.41cdND
    8ND0.74±0.02b0.49±0.01e5.40±0.13f3.74±0.20dND
    荧光假单胞菌0NDND0.28±0.04c7.53±0.42a2.65±0.27aND
    1NDND0.25±0.03c9.31±1.16a4.46±0.40a0.21±0.09a
    2NDND0.18±0.05ab5.68±1.06a4.77±0.65a5.73±0.95b
    3NDND0.23±0.03bc17.95±0.96ab5.27±0.24a10.29±1.12c
    4NDND0.16±0.02a27.50±1.59b23.50±2.42b16.95±1.38d
    5NDND0.16±0.04a45.58±3.82c33.72±2.60c26.46±3.28e
    6ND0.48±0.18a0.17±0.01a76.99±0.57d45.43±4.36d33.10±0.32f
    7ND0.75±0.06c0.26±0.02c138.83±4.88e54.34±0.59e45.89±1.36g
    8ND0.69±0.16b0.27±0.02c196.23±7.21f65.35±1.21f57.22±1.71h
    弗氏柠檬酸杆菌0NDND0.24±0.01bcd8.26±0.76a3.11±0.22aND
    1NDND0.24±0.03bcd9.22±0.59a5.60±0.33a0.30±0.22a
    2NDND0.15±0.02ab5.94±0.70a11.02±0.84b3.34±0.99ab
    3NDND0.27±0.12d14.08±0.62a15.74±3.12c8.67±0.86b
    4NDND0.16±0.02abc18.27±4.86ab20.60±2.68d18.45±1.27c
    5NDND0.14±0.02a28.71±1.63bc28.69±3.41e26.26±6.18d
    6ND0.49±0.03b0.16±0.02abc38.10±6.54c39.71±1.53f38.33±3.94e
    7ND0.28±0.47a0.24±0.01bcd50.67±2.66d43.93±3.52g45.45±0.93f
    8ND0.61±0.28c0.25±0.03cd83.43±7.92e55.49±4.24h55.39±2.65g
    嗜水气单胞菌0NDND0.24±0.03ab8.98±0.58a3.80±0.37aND
    1NDND0.22±0.02ab14.50±1.62a5.60±0.22a0.30±0.09a
    2NDND0.14±0.04a36.74±3.59b13.50±2.68b4.67±0.33b
    3NDND0.20±0.01ab53.12±1.67c19.58±3.09c8.34±1.07c
    4NDND0.26±0.05bc71.69±6.00d28.58±0.37d13.40±2.81d
    5NDND0.34±0.04c107.84±2.96e35.21±2.22e22.63±2.63e
    6NDND0.63±0.12d149.78±4.97f45.48±1.96f32.13±1.08f
    7NDND0.78±0.07e204.29±7.48g58.76±2.00g45.84±3.69g
    8ND0.25±0.040.86±0.04e261.22±11.42h69.99±2.83h54.81±3.01h
    注:ND表示未检出,同列不同小写字母表示同一组别不同贮藏时间之间存在显著性差异(P<0.05)。
    下载: 导出CSV
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  • 收稿日期:  2022-04-08
  • 网络出版日期:  2022-10-21
  • 刊出日期:  2022-11-23

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